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The map label for this gene is ispD

Identifier: 34496713

GI number: 34496713

Start: 1322903

End: 1323580

Strand: Direct

Name: ispD

Synonym: CV_1258

Alternate gene names: 34496713

Gene position: 1322903-1323580 (Clockwise)

Preceding gene: 34496712

Following gene: 34496714

Centisome position: 27.84

GC content: 71.68

Gene sequence:

>678_bases
ATGATCGCGCTGGTGCCGGCGGCCGGCTCCGGCAGCCGTTTCGGCGCGCCCAGCCCCAAGCAGTATCTGCAATTGAATGG
CAAGCCGCTGATGTGGCACACGCTGGCCACGGTGGCGGCGGTGCCCGATGTGGACGAAGTGGCGGTGGTGATTTCGCCGC
AGGACGAGTGGTTCGACGATTTCGCATGGGACTTGCCCAAGCTGTCGGTCCATCGCGTCGGCGGCGCCAGCCGCGCCCAG
AGCGTGGCGAGCGGCTTGGCCGCGCTGGCCTGCGCCGACGACGACTGGGTGCTGGTCCACGACGCCGCTCGCTGCTGCCT
GTCGGTGGCCGCCGTCGAGCGCCTGATCGCCGCGCTGTCCGGCCACGCGGTCGGCGGCTTGCTGGCGCTGCCGGTGCCGG
ACACCGTCAAGCGCGCCGACATCGACGGCCACGTCGCGGCCACCGTGCCGCGCAACGGCCTGTGGCTGGCGCAGACGCCG
CAGATGTTCCGCGCCGGCCTGCTGGCCAGGGCGTTGTCCAGCGCGGCGGCCGAGGACATCACCGACGAGGCGTCCGCCGT
CGAGCGGCTGGGCGTGAAACCGCTGCTGGTGGAGGGCGACGCGCAGAACTTCAAGATCACCTATCCGCGCGATCTGGCGC
TGGCCCGGGCCATCCTGGCCGCGCGCGACGAATACTGA

Upstream 100 bases:

>100_bases
CAAGCCTATCTTGACGTGCTGGACAAGGCGGTCAAGGGCATCTGCGTCTGGCGCGGGCTGGCGTCGCCGGCGCAGGCGGA
GGGCTGAGGCGATGGCCAGA

Downstream 100 bases:

>100_bases
ATCAGGCAAGGAAGAACATGTTTCGCATCGGACAAGGCTACGACGTGCACCAGCTGGTGGAAGGCCGGCCGCTGATCCTG
GGCGGGGTGGACATTCCGCA

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MIALVPAAGSGSRFGAPSPKQYLQLNGKPLMWHTLATVAAVPDVDEVAVVISPQDEWFDDFAWDLPKLSVHRVGGASRAQ
SVASGLAALACADDDWVLVHDAARCCLSVAAVERLIAALSGHAVGGLLALPVPDTVKRADIDGHVAATVPRNGLWLAQTP
QMFRAGLLARALSSAAAEDITDEASAVERLGVKPLLVEGDAQNFKITYPRDLALARAILAARDEY

Sequences:

>Translated_225_residues
MIALVPAAGSGSRFGAPSPKQYLQLNGKPLMWHTLATVAAVPDVDEVAVVISPQDEWFDDFAWDLPKLSVHRVGGASRAQ
SVASGLAALACADDDWVLVHDAARCCLSVAAVERLIAALSGHAVGGLLALPVPDTVKRADIDGHVAATVPRNGLWLAQTP
QMFRAGLLARALSSAAAEDITDEASAVERLGVKPLLVEGDAQNFKITYPRDLALARAILAARDEY
>Mature_225_residues
MIALVPAAGSGSRFGAPSPKQYLQLNGKPLMWHTLATVAAVPDVDEVAVVISPQDEWFDDFAWDLPKLSVHRVGGASRAQ
SVASGLAALACADDDWVLVHDAARCCLSVAAVERLIAALSGHAVGGLLALPVPDTVKRADIDGHVAATVPRNGLWLAQTP
QMFRAGLLARALSSAAAEDITDEASAVERLGVKPLLVEGDAQNFKITYPRDLALARAILAARDEY

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=235, Percent_Identity=26.3829787234043, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1789104, Length=223, Percent_Identity=46.1883408071749, Blast_Score=159, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_CHRVO (Q7NYL6)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_900928.1
- ProteinModelPortal:   Q7NYL6
- SMR:   Q7NYL6
- GeneID:   2550733
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1258
- NMPDR:   fig|243365.1.peg.1258
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- PhylomeDB:   Q7NYL6
- BioCyc:   CVIO243365:CV_1258-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 23678; Mature: 23678

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALVPAAGSGSRFGAPSPKQYLQLNGKPLMWHTLATVAAVPDVDEVAVVISPQDEWFDD
CEEEEECCCCCCCCCCCCCHHHEEECCCEEHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
FAWDLPKLSVHRVGGASRAQSVASGLAALACADDDWVLVHDAARCCLSVAAVERLIAALS
HHHCCCCCEEEECCCHHHHHHHHHHHHEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHC
GHAVGGLLALPVPDTVKRADIDGHVAATVPRNGLWLAQTPQMFRAGLLARALSSAAAEDI
CCCCCCEEEECCCCHHHHCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
TDEASAVERLGVKPLLVEGDAQNFKITYPRDLALARAILAARDEY
HHHHHHHHHHCCCEEEEECCCCCEEEECCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIALVPAAGSGSRFGAPSPKQYLQLNGKPLMWHTLATVAAVPDVDEVAVVISPQDEWFDD
CEEEEECCCCCCCCCCCCCHHHEEECCCEEHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
FAWDLPKLSVHRVGGASRAQSVASGLAALACADDDWVLVHDAARCCLSVAAVERLIAALS
HHHCCCCCEEEECCCHHHHHHHHHHHHEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHC
GHAVGGLLALPVPDTVKRADIDGHVAATVPRNGLWLAQTPQMFRAGLLARALSSAAAEDI
CCCCCCEEEECCCCHHHHCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
TDEASAVERLGVKPLLVEGDAQNFKITYPRDLALARAILAARDEY
HHHHHHHHHHCCCEEEEECCCCCEEEECCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782