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The map label for this gene is ispF

Identifier: 34496714

GI number: 34496714

Start: 1323598

End: 1324074

Strand: Direct

Name: ispF

Synonym: CV_1259

Alternate gene names: 34496714

Gene position: 1323598-1324074 (Clockwise)

Preceding gene: 34496713

Following gene: 34496715

Centisome position: 27.86

GC content: 71.28

Gene sequence:

>477_bases
ATGTTTCGCATCGGACAAGGCTACGACGTGCACCAGCTGGTGGAAGGCCGGCCGCTGATCCTGGGCGGGGTGGACATTCC
GCACGACAAGGGCCTGCTCGGCCATTCCGACGCCGACGCGCTGCTGCACGCGATCACCGACGCGCTGTTGGGCGCGGCGG
CGCTGGGCGACATCGGCCGCCATTTCCCGGACACCGCCGCCGAGTTCAAGGGGGCGGACAGCCGCGCGCTGCTGCGCGAG
GCGGCCGCGCGGGTGCGCGCCGCCGGCTGGCGTCCGGTCAACGTCGACAGCACGCTGATCGCGCAGCGGCCCAAGCTGGC
GCCTCACATCGACGCGATGCGCGCCAACATCGCCGCCGACCTGGGGCTGGATGTCGGGGCGGTCAACGTCAAGGGCAAGA
CCAACGAGAAGCTGGGGTACCTGGGACGCTGCGAGGCGATAGAGGCCCAGGCCGTCTGCCTGCTGGCCCAGGCCTAG

Upstream 100 bases:

>100_bases
GCGACGCGCAGAACTTCAAGATCACCTATCCGCGCGATCTGGCGCTGGCCCGGGCCATCCTGGCCGCGCGCGACGAATAC
TGAATCAGGCAAGGAAGAAC

Downstream 100 bases:

>100_bases
AGCATTGCCTGCCGCCGATGCCGCCGCCCGCGCCCGCGGTTCGAGCGGCGGCGTTGAAAGACCTTAGAACAAGCGGCCAG
GCATCATGCCGGCCAAGTCC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MFRIGQGYDVHQLVEGRPLILGGVDIPHDKGLLGHSDADALLHAITDALLGAAALGDIGRHFPDTAAEFKGADSRALLRE
AAARVRAAGWRPVNVDSTLIAQRPKLAPHIDAMRANIAADLGLDVGAVNVKGKTNEKLGYLGRCEAIEAQAVCLLAQA

Sequences:

>Translated_158_residues
MFRIGQGYDVHQLVEGRPLILGGVDIPHDKGLLGHSDADALLHAITDALLGAAALGDIGRHFPDTAAEFKGADSRALLRE
AAARVRAAGWRPVNVDSTLIAQRPKLAPHIDAMRANIAADLGLDVGAVNVKGKTNEKLGYLGRCEAIEAQAVCLLAQA
>Mature_158_residues
MFRIGQGYDVHQLVEGRPLILGGVDIPHDKGLLGHSDADALLHAITDALLGAAALGDIGRHFPDTAAEFKGADSRALLRE
AAARVRAAGWRPVNVDSTLIAQRPKLAPHIDAMRANIAADLGLDVGAVNVKGKTNEKLGYLGRCEAIEAQAVCLLAQA

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=60.5095541401274, Blast_Score=170, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_CHRVO (Q7NYL5)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_900929.1
- ProteinModelPortal:   Q7NYL5
- SMR:   Q7NYL5
- GeneID:   2550716
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1259
- NMPDR:   fig|243365.1.peg.1259
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   Q7NYL5
- ProtClustDB:   PRK00084
- BioCyc:   CVIO243365:CV_1259-MONOMER
- BRENDA:   4.6.1.12
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16600; Mature: 16600

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRIGQGYDVHQLVEGRPLILGGVDIPHDKGLLGHSDADALLHAITDALLGAAALGDIGR
CCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HFPDTAAEFKGADSRALLREAAARVRAAGWRPVNVDSTLIAQRPKLAPHIDAMRANIAAD
CCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHH
LGLDVGAVNVKGKTNEKLGYLGRCEAIEAQAVCLLAQA
CCCCEEEEEECCCCCCCCCCCCCCCHHCHHEEEEEECC
>Mature Secondary Structure
MFRIGQGYDVHQLVEGRPLILGGVDIPHDKGLLGHSDADALLHAITDALLGAAALGDIGR
CCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HFPDTAAEFKGADSRALLREAAARVRAAGWRPVNVDSTLIAQRPKLAPHIDAMRANIAAD
CCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHH
LGLDVGAVNVKGKTNEKLGYLGRCEAIEAQAVCLLAQA
CCCCEEEEEECCCCCCCCCCCCCCCHHCHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782