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The map label for this gene is dnaQ [H]

Identifier: 34496712

GI number: 34496712

Start: 1322170

End: 1322889

Strand: Direct

Name: dnaQ [H]

Synonym: CV_1257

Alternate gene names: 34496712

Gene position: 1322170-1322889 (Clockwise)

Preceding gene: 34496711

Following gene: 34496713

Centisome position: 27.83

GC content: 65.14

Gene sequence:

>720_bases
ATGAGACAGATTATTCTCGATACCGAAACCACCGGTCTCGATCCGCAGCAAGGCCACCGCATCATTGAATTCGCCGGCCT
GGAGATGGTCGGCCGCAAGCTGACCGGCAAACACCTGCATCTTTATATACATCCGGAACGCGAGATCGACCCGGAGGCCC
AGCGCGTGCACGGCATTTCGCTGGAGTTTCTGGCGGGCAAGCCTGTGTTCGCCAAGGTGGCGCACGAGATCGCGGACTTC
CTGCGCGACGCCGAGCTGATCATTCACAACGCGCCCTTCGACGTCGGCTTCCTCAACGCCGAGTTCGCCAAGGCCGGCAT
CGAGCCGGTGGGCAAGCTGTGCGCCAGCGTGATCGACACGCTGGCCGAGGCGCGCGACATGTTCCCCGGCAAGCGCAACA
GCCTGGACGCCTTGTGCGACCGCTTCGAGATCGACCGTTCCAACCGGACGCTGCACGGCGCGCTGGTGGACTGCGAGCTG
CTGTCCGAAGTGTATCTATGGATGACGCGCGGGCAGGAAAGCCTGGCGATGGACATCGAGGTGGAGCTGCCGGGCGGCGA
CGCCGGGGCCATCCAGTTCGAGCGCAAGCCGCTGAAGGTGCTGGCCGCCAGCGAGGCCGAAGAGGCCGAGCACCAAGCCT
ATCTTGACGTGCTGGACAAGGCGGTCAAGGGCATCTGCGTCTGGCGCGGGCTGGCGTCGCCGGCGCAGGCGGAGGGCTGA

Upstream 100 bases:

>100_bases
AACGAGAGGGCCGACCAGCTCGCCAACAAGGGCGTGGAGTCGGTCTGAACAGTAGACACGCCGCGGCGCCGTCGATACGG
CGCCGCGGGCAGGACGCAGC

Downstream 100 bases:

>100_bases
GGCGATGGCCAGAATGATCGCGCTGGTGCCGGCGGCCGGCTCCGGCAGCCGTTTCGGCGCGCCCAGCCCCAAGCAGTATC
TGCAATTGAATGGCAAGCCG

Product: DNA-directed DNA polymerase subunit epsilon

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRQIILDTETTGLDPQQGHRIIEFAGLEMVGRKLTGKHLHLYIHPEREIDPEAQRVHGISLEFLAGKPVFAKVAHEIADF
LRDAELIIHNAPFDVGFLNAEFAKAGIEPVGKLCASVIDTLAEARDMFPGKRNSLDALCDRFEIDRSNRTLHGALVDCEL
LSEVYLWMTRGQESLAMDIEVELPGGDAGAIQFERKPLKVLAASEAEEAEHQAYLDVLDKAVKGICVWRGLASPAQAEG

Sequences:

>Translated_239_residues
MRQIILDTETTGLDPQQGHRIIEFAGLEMVGRKLTGKHLHLYIHPEREIDPEAQRVHGISLEFLAGKPVFAKVAHEIADF
LRDAELIIHNAPFDVGFLNAEFAKAGIEPVGKLCASVIDTLAEARDMFPGKRNSLDALCDRFEIDRSNRTLHGALVDCEL
LSEVYLWMTRGQESLAMDIEVELPGGDAGAIQFERKPLKVLAASEAEEAEHQAYLDVLDKAVKGICVWRGLASPAQAEG
>Mature_239_residues
MRQIILDTETTGLDPQQGHRIIEFAGLEMVGRKLTGKHLHLYIHPEREIDPEAQRVHGISLEFLAGKPVFAKVAHEIADF
LRDAELIIHNAPFDVGFLNAEFAKAGIEPVGKLCASVIDTLAEARDMFPGKRNSLDALCDRFEIDRSNRTLHGALVDCEL
LSEVYLWMTRGQESLAMDIEVELPGGDAGAIQFERKPLKVLAASEAEEAEHQAYLDVLDKAVKGICVWRGLASPAQAEG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786409, Length=239, Percent_Identity=51.4644351464435, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006054
- InterPro:   IPR006309
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 26376; Mature: 26376

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQIILDTETTGLDPQQGHRIIEFAGLEMVGRKLTGKHLHLYIHPEREIDPEAQRVHGIS
CCEEEEECCCCCCCHHHCCEEEEEHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHCCCE
LEFLAGKPVFAKVAHEIADFLRDAELIIHNAPFDVGFLNAEFAKAGIEPVGKLCASVIDT
EEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEECCHHHHHCCCCHHHHHHHHHHHH
LAEARDMFPGKRNSLDALCDRFEIDRSNRTLHGALVDCELLSEVYLWMTRGQESLAMDIE
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEE
VELPGGDAGAIQFERKPLKVLAASEAEEAEHQAYLDVLDKAVKGICVWRGLASPAQAEG
EEECCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRQIILDTETTGLDPQQGHRIIEFAGLEMVGRKLTGKHLHLYIHPEREIDPEAQRVHGIS
CCEEEEECCCCCCCHHHCCEEEEEHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHCCCE
LEFLAGKPVFAKVAHEIADFLRDAELIIHNAPFDVGFLNAEFAKAGIEPVGKLCASVIDT
EEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEECCHHHHHCCCCHHHHHHHHHHHH
LAEARDMFPGKRNSLDALCDRFEIDRSNRTLHGALVDCELLSEVYLWMTRGQESLAMDIE
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEE
VELPGGDAGAIQFERKPLKVLAASEAEEAEHQAYLDVLDKAVKGICVWRGLASPAQAEG
EEECCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3023634; 6316347; 3540531; 9278503; 1575709 [H]