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The map label for this gene is gloB [H]

Identifier: 34496709

GI number: 34496709

Start: 1320118

End: 1320900

Strand: Reverse

Name: gloB [H]

Synonym: CV_1254

Alternate gene names: 34496709

Gene position: 1320900-1320118 (Counterclockwise)

Preceding gene: 34496719

Following gene: 34496708

Centisome position: 27.8

GC content: 66.67

Gene sequence:

>783_bases
ATGGGGGAAAGCCTTATGGCAGCCATGTTTAGCATCGCCCCGGTTGGGGCATTCACTGACAATTATATCTGGGTTTTGCG
CGAGGGCGCGCGCGCCGTCGCCGTCGACCCGGGCGAAGCCGCGCCGCTGCTGCGCCATCTGGAGCAGGAACAGCTGCGGC
TGGAAGCCGTTCTGATCACCCACCATCACGCCGACCACTGCGGCGGACTGGCGGATGTGCTGCGCGCCTGGCCGGGCATC
CCGGTCTACGGCCCGGCCGGCCTTCCCGGCGTCAGCCATCCGGTGATGGAAGGCGATGCCGTCTCTCTGAGCATGGGACG
GGCCGAAGTGCTGGAAGTGCCCGGCCATACGCTGGACCATCTTGCATACCTGCTGGAAGACGCGCTGTTTTGCGGGGACA
CCCTGTTCGCGGCCGGCTGCGGCAGGCTATTCGAGGGGACGCCGGGACAGATGCTGGCCTCGCTGAAAAAGCTGGCCGGA
TTGCCGCCCAACACCAAGGTTTACCCGGCCCACGAATACACGCTGTCGAATCTGCGCTTCGCGCTGGCTGCCGATCAGCA
TAATCCGATACTCGCGATGCGCCACAGCCGCGACAGCGCGCTGCGGGATCGCGGACTGCCGACGCTGCCCAGCACCATCG
CGCTGGAAACCGCCAGCAACCCCTTCTTGCGCAGCGGCGAGCCGGCGCTGCGCGAAGCGCTGCGGCGGCGCGGCGGGCAG
CCCGGCGACAGCGAGGAAACCTGCTTCGCGCTGCTGAGGGAATGGAAGAACGATTTCCGTTGA

Upstream 100 bases:

>100_bases
GAAGGCATCGGCCACCGCGCGCTCGAAATACGCCTGCTCGCGTTCAAGCAGGTAGCGTCCGAGCTCGGTGCCGGTCAGCC
AGGCGCCAAACGAATTTTTC

Downstream 100 bases:

>100_bases
AAGGCATGCCGGATACGTAAGTTTTCGCAGTCCGCCCGCCAGGCCCTTCACTTGCTCGTGAAATGAGCTATAAGCAATGA
AAGCGCATGAAATGATTTGG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGESLMAAMFSIAPVGAFTDNYIWVLREGARAVAVDPGEAAPLLRHLEQEQLRLEAVLITHHHADHCGGLADVLRAWPGI
PVYGPAGLPGVSHPVMEGDAVSLSMGRAEVLEVPGHTLDHLAYLLEDALFCGDTLFAAGCGRLFEGTPGQMLASLKKLAG
LPPNTKVYPAHEYTLSNLRFALAADQHNPILAMRHSRDSALRDRGLPTLPSTIALETASNPFLRSGEPALREALRRRGGQ
PGDSEETCFALLREWKNDFR

Sequences:

>Translated_260_residues
MGESLMAAMFSIAPVGAFTDNYIWVLREGARAVAVDPGEAAPLLRHLEQEQLRLEAVLITHHHADHCGGLADVLRAWPGI
PVYGPAGLPGVSHPVMEGDAVSLSMGRAEVLEVPGHTLDHLAYLLEDALFCGDTLFAAGCGRLFEGTPGQMLASLKKLAG
LPPNTKVYPAHEYTLSNLRFALAADQHNPILAMRHSRDSALRDRGLPTLPSTIALETASNPFLRSGEPALREALRRRGGQ
PGDSEETCFALLREWKNDFR
>Mature_259_residues
GESLMAAMFSIAPVGAFTDNYIWVLREGARAVAVDPGEAAPLLRHLEQEQLRLEAVLITHHHADHCGGLADVLRAWPGIP
VYGPAGLPGVSHPVMEGDAVSLSMGRAEVLEVPGHTLDHLAYLLEDALFCGDTLFAAGCGRLFEGTPGQMLASLKKLAGL
PPNTKVYPAHEYTLSNLRFALAADQHNPILAMRHSRDSALRDRGLPTLPSTIALETASNPFLRSGEPALREALRRRGGQP
GDSEETCFALLREWKNDFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI116642887, Length=260, Percent_Identity=37.3076923076923, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI21703352, Length=257, Percent_Identity=37.7431906614786, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI94538320, Length=255, Percent_Identity=33.7254901960784, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI94538322, Length=255, Percent_Identity=33.7254901960784, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI14150041, Length=233, Percent_Identity=37.7682403433476, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI46361987, Length=189, Percent_Identity=39.1534391534392, Blast_Score=111, Evalue=8e-25,
Organism=Escherichia coli, GI1786406, Length=254, Percent_Identity=41.3385826771654, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1787158, Length=211, Percent_Identity=32.7014218009479, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536925, Length=259, Percent_Identity=34.3629343629344, Blast_Score=144, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6320478, Length=240, Percent_Identity=30.8333333333333, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6324614, Length=238, Percent_Identity=29.8319327731092, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356335, Length=242, Percent_Identity=35.9504132231405, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24667711, Length=242, Percent_Identity=35.9504132231405, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24667703, Length=242, Percent_Identity=35.9504132231405, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI221330176, Length=185, Percent_Identity=30.8108108108108, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28141; Mature: 28010

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGESLMAAMFSIAPVGAFTDNYIWVLREGARAVAVDPGEAAPLLRHLEQEQLRLEAVLIT
CCHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHEEE
HHHADHCGGLADVLRAWPGIPVYGPAGLPGVSHPVMEGDAVSLSMGRAEVLEVPGHTLDH
CCCCHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHEEECCCCHHHH
LAYLLEDALFCGDTLFAAGCGRLFEGTPGQMLASLKKLAGLPPNTKVYPAHEYTLSNLRF
HHHHHHHHHHHCHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCEEECCHHHHHHHEEE
ALAADQHNPILAMRHSRDSALRDRGLPTLPSTIALETASNPFLRSGEPALREALRRRGGQ
EEEECCCCCEEEEECCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCC
PGDSEETCFALLREWKNDFR
CCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GESLMAAMFSIAPVGAFTDNYIWVLREGARAVAVDPGEAAPLLRHLEQEQLRLEAVLIT
CHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHEEE
HHHADHCGGLADVLRAWPGIPVYGPAGLPGVSHPVMEGDAVSLSMGRAEVLEVPGHTLDH
CCCCHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHEEECCCCHHHH
LAYLLEDALFCGDTLFAAGCGRLFEGTPGQMLASLKKLAGLPPNTKVYPAHEYTLSNLRF
HHHHHHHHHHHCHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCEEECCHHHHHHHEEE
ALAADQHNPILAMRHSRDSALRDRGLPTLPSTIALETASNPFLRSGEPALREALRRRGGQ
EEEECCCCCEEEEECCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCC
PGDSEETCFALLREWKNDFR
CCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA