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The map label for this gene is pncA [H]

Identifier: 34496719

GI number: 34496719

Start: 1328492

End: 1329124

Strand: Reverse

Name: pncA [H]

Synonym: CV_1264

Alternate gene names: 34496719

Gene position: 1329124-1328492 (Counterclockwise)

Preceding gene: 34496722

Following gene: 34496709

Centisome position: 27.98

GC content: 66.19

Gene sequence:

>633_bases
ATGCCCCTGAAACGTTATGGTGCCGACTGCGCATTGCTGGTGGTGGATGTGCAGAACAGTTTCTGTCCCGGCGGCGAACT
GGCCGTGCCGGGCGGAGACGAGGTGGCGCCCTTGATCAACCACCTGTCGCTGCTGTTCGAAAACGTGGTGCTGACCCAGG
ACTGGCACCCGGCGGGCCATATATCGTTCGCCTCTAGCCATCCCGGCATGCAGCCCTTCCAGAGCGTGGATCTGCCCTAC
GGCCCGCAGACGCTGTGGCCGGACCATTGCGTCGCCGGCAGCCATGGCGCGGACTTCCACCCGGAACTGGAAACCCAGCA
TGCCCGGCTGATCGTGCGCAAAGGCATCCACGCCAAGGTGGACAGCTATTCCGCCTTCGTCGAGGCAGACCGCGCTGCCA
GCACCGGCCTGGCCGGCTACCTGCGCGAGCTGGGCGTGAAAAAGGTGTGGCTGGCCGGCCTCGCCACCGACTTCTGCGTC
GCCTGGAGCGCCATCGACGCCTGCGCCGCCGGCTTCGAAACCTTCGTGGTGGAGGATGCCTGCCGCGCGATAGACATCGA
CGGCTCGCTGGCAGACGCCTGGATGAAGATGCGCCAGGCCGGCGTCAAGCGGCTGCGATCCGACGAGGTGTGA

Upstream 100 bases:

>100_bases
CACGGGGTATTAAACGCAAGCATGGAAAGCCCCGCGCCCCCTTTACCGGCAGTCCCGCCCGTGTTAACAAGATGGGAGAC
GACAACAACTAGGTAGTCCC

Downstream 100 bases:

>100_bases
AGCCGAAATAAAAACGGGGAGAATCGCTCTCCCCGTTTTCCCGTCCCGGCCGCGGGATCAGATCCAGCGCCGCTTCCAGA
ACCACCAACCCATGCCGGCG

Product: bifunctional pyrazinamidase/nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MPLKRYGADCALLVVDVQNSFCPGGELAVPGGDEVAPLINHLSLLFENVVLTQDWHPAGHISFASSHPGMQPFQSVDLPY
GPQTLWPDHCVAGSHGADFHPELETQHARLIVRKGIHAKVDSYSAFVEADRAASTGLAGYLRELGVKKVWLAGLATDFCV
AWSAIDACAAGFETFVVEDACRAIDIDGSLADAWMKMRQAGVKRLRSDEV

Sequences:

>Translated_210_residues
MPLKRYGADCALLVVDVQNSFCPGGELAVPGGDEVAPLINHLSLLFENVVLTQDWHPAGHISFASSHPGMQPFQSVDLPY
GPQTLWPDHCVAGSHGADFHPELETQHARLIVRKGIHAKVDSYSAFVEADRAASTGLAGYLRELGVKKVWLAGLATDFCV
AWSAIDACAAGFETFVVEDACRAIDIDGSLADAWMKMRQAGVKRLRSDEV
>Mature_209_residues
PLKRYGADCALLVVDVQNSFCPGGELAVPGGDEVAPLINHLSLLFENVVLTQDWHPAGHISFASSHPGMQPFQSVDLPYG
PQTLWPDHCVAGSHGADFHPELETQHARLIVRKGIHAKVDSYSAFVEADRAASTGLAGYLRELGVKKVWLAGLATDFCVA
WSAIDACAAGFETFVVEDACRAIDIDGSLADAWMKMRQAGVKRLRSDEV

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=196, Percent_Identity=43.8775510204082, Blast_Score=157, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI193206735, Length=224, Percent_Identity=33.4821428571429, Blast_Score=106, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17543130, Length=224, Percent_Identity=33.4821428571429, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71999339, Length=199, Percent_Identity=33.6683417085427, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321401, Length=198, Percent_Identity=34.3434343434343, Blast_Score=105, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648247, Length=200, Percent_Identity=35, Blast_Score=113, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22671; Mature: 22540

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLKRYGADCALLVVDVQNSFCPGGELAVPGGDEVAPLINHLSLLFENVVLTQDWHPAGH
CCCCHHCCCEEEEEEECCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
ISFASSHPGMQPFQSVDLPYGPQTLWPDHCVAGSHGADFHPELETQHARLIVRKGIHAKV
EEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCH
DSYSAFVEADRAASTGLAGYLRELGVKKVWLAGLATDFCVAWSAIDACAAGFETFVVEDA
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CRAIDIDGSLADAWMKMRQAGVKRLRSDEV
HHEECCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PLKRYGADCALLVVDVQNSFCPGGELAVPGGDEVAPLINHLSLLFENVVLTQDWHPAGH
CCCHHCCCEEEEEEECCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
ISFASSHPGMQPFQSVDLPYGPQTLWPDHCVAGSHGADFHPELETQHARLIVRKGIHAKV
EEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCH
DSYSAFVEADRAASTGLAGYLRELGVKKVWLAGLATDFCVAWSAIDACAAGFETFVVEDA
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CRAIDIDGSLADAWMKMRQAGVKRLRSDEV
HHEECCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]