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The map label for this gene is mltD [H]

Identifier: 34496708

GI number: 34496708

Start: 1318086

End: 1319948

Strand: Reverse

Name: mltD [H]

Synonym: CV_1253

Alternate gene names: 34496708

Gene position: 1319948-1318086 (Counterclockwise)

Preceding gene: 34496709

Following gene: 34496705

Centisome position: 27.78

GC content: 66.56

Gene sequence:

>1863_bases
ATGAAACGCTTCACTCCATTGGCACTTGCCGTGTCGCTCGCATTGCCGTTCGCATCGTCGGTCCTGGCCGGCGCCGCGCC
GTCTTCCGGCGTGGACGAGGCGATGGCCGCCGGCCTCGACATGATGCTGCTGAACTCCAGCCTGCTGCGCAACGGCGACG
ACGTCTGGAAGCGCGCCCGCGAAGGCTTTCAGCTGGACGAGGTCAACGCCGACACGGTCCGTCGCCAGGAGCGCTTTTAC
GCCAGCCGGCCCGAATATTTCAAACGCACGCTGGACCGCAGCCGCAAATACCTGTTCCACATCATGAACGAAGTGGAGCG
GCGCGGCATGCCGACCGAGATCGCGCTGTTGCCGATGGTGGAAAGCGCCTTCGTCCCCACCGCCAACTCCCGCGTCGGCG
CCGCCGGCCTGTGGCAGTTCATGCCGGCCACCGGCCGCCATTACGGGCTGGAGCAAACCTGGTGGTACGACGGCCGCCGC
GACGTCACGGACGCCACCCGCGCCGCGCTGGATTATCTGCAGAACCTGTACGCCCAGTTCGGCGACTGGAACCTGGCGCT
GGCCGCGTACAACTGGGGCGAGGGCAACCTGGCCCGCGCGATCGCCAAGGCCCAGGCCAGCGGCATCGAACCCACTTATG
AAAACATCCGCCTGCCGAACGAGACCCGCAACTACGTGCCCAAGCTGCTGGCGGTGCGCAACATCCTGCTGAATCCGGAA
AAGTTCGGCGTCCACCTCGACAAATTCCCCAACAAGCCCTACTTCGTCGCCGTCTCCACCGGCAAGCACATGAACATCGA
CATCGCCGCCAAGCTGGCTGGCATCTCGGTGGCGGAATTCAAGGAATTGAATCCGGCGTTCAATCTGCCGGTATTCGCCT
ACAAGGCCGGCCGCCAGATGCTGCTGCCGGCGTCCAAGGTCGACAAGTTTCAGGCCAATCTGGACAAGTGGAGCAAACCG
CTGCTGACTTGGGAAGTTTACATGCCCAAGAGCGACGAGAGCGTCGGAGCCGTCGCCGGCGACCACGGCATGAGCGCGGG
CCAGCTGCTCGCGGCCAACCGCATCAGCGGCGGCACGCTGAAGGCCGGCCAGCCGGTGCTGGTGGCGATGAACAAGCCGC
TGAACGACGGACAATCTTTCGAATCGGTCGACACCCCGATAGGCGACGCCCCGGCCAACGCCGGCGCGACGATGATCGCG
CAAGCCGAACCCGCGCCGATATCCGCGCCCGCGGCCCAGCCGGCCATGACGCAGCTGGCGCTGGCCGCAACCGCGCAGAC
GACCGCTCCGGCGCAAAACAATGCCGCGACCGCCTCTCCGGCCGCCATAGTCGCCTCCGCGCAGCCCCAGGTCGCGCCGC
AGCCCGCCGCAGACGCAGGCCTCGCCGCCAGGCAAGCCGTGCTGCTGGCCAGTTCGGCAAGCAGCGCCGAAGACAATGCG
GCCGCCAGATCCCTTGCCACCCGCGATCTCGGCAGCTACACCGTCGCCAGCGGCGACACGCTGTACAGCATCGCCCGCCG
CAGCAACCTCAGCATCGACGACCTGAGAACGCTGAACCAGCTGGACGGCAATCTGGTCCAGGTGGGCCAGAAGCTGAAGC
TGAAGGCCGACGGCGCGGCCGACAACCTCGTCGCCAGCAACGGCGGCGATGGCGCCGATCCCGCGCTGGTCAAGGTATCC
GAGACCCGCCCGGCAGCCAGCCACGCCCGCCGCAGCAAAGAATACGTGGTCCAGCGCGGCGACACCCTGTTCAGCATCGC
CCGCCGTTTCGGCGTCACCCACAACGATATCCAGCGCATCAACGGCAGCCGGCACCCCAGCCACCTGCAACCAGGCCAAA
AGGTCAAGATCGTCGGCCTGTAA

Upstream 100 bases:

>100_bases
ATAAGCAATGAAAGCGCATGAAATGATTTGGCTTTTTCGATTGACACCCCCCTGGCGCATGTTTAGCATCAGCCGGAATC
AGAATTACGGGCCCGTCTGA

Downstream 100 bases:

>100_bases
GCGCAGACTCCCGCATGAAAACGGCCCGGAACTCCGGGCCGTTTCTGATTGGATGATCAGGTTTTCCTCGGCGCGCCGCG
CATCCGCCGCACCAGGCTGC

Product: peptidoglycan N-acetylmuramoylhydrolase

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MKRFTPLALAVSLALPFASSVLAGAAPSSGVDEAMAAGLDMMLLNSSLLRNGDDVWKRAREGFQLDEVNADTVRRQERFY
ASRPEYFKRTLDRSRKYLFHIMNEVERRGMPTEIALLPMVESAFVPTANSRVGAAGLWQFMPATGRHYGLEQTWWYDGRR
DVTDATRAALDYLQNLYAQFGDWNLALAAYNWGEGNLARAIAKAQASGIEPTYENIRLPNETRNYVPKLLAVRNILLNPE
KFGVHLDKFPNKPYFVAVSTGKHMNIDIAAKLAGISVAEFKELNPAFNLPVFAYKAGRQMLLPASKVDKFQANLDKWSKP
LLTWEVYMPKSDESVGAVAGDHGMSAGQLLAANRISGGTLKAGQPVLVAMNKPLNDGQSFESVDTPIGDAPANAGATMIA
QAEPAPISAPAAQPAMTQLALAATAQTTAPAQNNAATASPAAIVASAQPQVAPQPAADAGLAARQAVLLASSASSAEDNA
AARSLATRDLGSYTVASGDTLYSIARRSNLSIDDLRTLNQLDGNLVQVGQKLKLKADGAADNLVASNGGDGADPALVKVS
ETRPAASHARRSKEYVVQRGDTLFSIARRFGVTHNDIQRINGSRHPSHLQPGQKVKIVGL

Sequences:

>Translated_620_residues
MKRFTPLALAVSLALPFASSVLAGAAPSSGVDEAMAAGLDMMLLNSSLLRNGDDVWKRAREGFQLDEVNADTVRRQERFY
ASRPEYFKRTLDRSRKYLFHIMNEVERRGMPTEIALLPMVESAFVPTANSRVGAAGLWQFMPATGRHYGLEQTWWYDGRR
DVTDATRAALDYLQNLYAQFGDWNLALAAYNWGEGNLARAIAKAQASGIEPTYENIRLPNETRNYVPKLLAVRNILLNPE
KFGVHLDKFPNKPYFVAVSTGKHMNIDIAAKLAGISVAEFKELNPAFNLPVFAYKAGRQMLLPASKVDKFQANLDKWSKP
LLTWEVYMPKSDESVGAVAGDHGMSAGQLLAANRISGGTLKAGQPVLVAMNKPLNDGQSFESVDTPIGDAPANAGATMIA
QAEPAPISAPAAQPAMTQLALAATAQTTAPAQNNAATASPAAIVASAQPQVAPQPAADAGLAARQAVLLASSASSAEDNA
AARSLATRDLGSYTVASGDTLYSIARRSNLSIDDLRTLNQLDGNLVQVGQKLKLKADGAADNLVASNGGDGADPALVKVS
ETRPAASHARRSKEYVVQRGDTLFSIARRFGVTHNDIQRINGSRHPSHLQPGQKVKIVGL
>Mature_620_residues
MKRFTPLALAVSLALPFASSVLAGAAPSSGVDEAMAAGLDMMLLNSSLLRNGDDVWKRAREGFQLDEVNADTVRRQERFY
ASRPEYFKRTLDRSRKYLFHIMNEVERRGMPTEIALLPMVESAFVPTANSRVGAAGLWQFMPATGRHYGLEQTWWYDGRR
DVTDATRAALDYLQNLYAQFGDWNLALAAYNWGEGNLARAIAKAQASGIEPTYENIRLPNETRNYVPKLLAVRNILLNPE
KFGVHLDKFPNKPYFVAVSTGKHMNIDIAAKLAGISVAEFKELNPAFNLPVFAYKAGRQMLLPASKVDKFQANLDKWSKP
LLTWEVYMPKSDESVGAVAGDHGMSAGQLLAANRISGGTLKAGQPVLVAMNKPLNDGQSFESVDTPIGDAPANAGATMIA
QAEPAPISAPAAQPAMTQLALAATAQTTAPAQNNAATASPAAIVASAQPQVAPQPAADAGLAARQAVLLASSASSAEDNA
AARSLATRDLGSYTVASGDTLYSIARRSNLSIDDLRTLNQLDGNLVQVGQKLKLKADGAADNLVASNGGDGADPALVKVS
ETRPAASHARRSKEYVVQRGDTLFSIARRFGVTHNDIQRINGSRHPSHLQPGQKVKIVGL

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=328, Percent_Identity=34.1463414634146, Blast_Score=194, Evalue=2e-50,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 66498; Mature: 66498

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFTPLALAVSLALPFASSVLAGAAPSSGVDEAMAAGLDMMLLNSSLLRNGDDVWKRAR
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHCCHHHHHHHH
EGFQLDEVNADTVRRQERFYASRPEYFKRTLDRSRKYLFHIMNEVERRGMPTEIALLPMV
CCCEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHH
ESAFVPTANSRVGAAGLWQFMPATGRHYGLEQTWWYDGRRDVTDATRAALDYLQNLYAQF
HHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
GDWNLALAAYNWGEGNLARAIAKAQASGIEPTYENIRLPNETRNYVPKLLAVRNILLNPE
CCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCH
KFGVHLDKFPNKPYFVAVSTGKHMNIDIAAKLAGISVAEFKELNPAFNLPVFAYKAGRQM
HHCEEHHCCCCCCEEEEEECCCEEEEEEEHHHHCCCHHHHHHCCCCCCCCEEEEECCCEE
LLPASKVDKFQANLDKWSKPLLTWEVYMPKSDESVGAVAGDHGMSAGQLLAANRISGGTL
ECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEECCCCCCCCCEEEECCCCCCCC
KAGQPVLVAMNKPLNDGQSFESVDTPIGDAPANAGATMIAQAEPAPISAPAAQPAMTQLA
CCCCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHH
LAATAQTTAPAQNNAATASPAAIVASAQPQVAPQPAADAGLAARQAVLLASSASSAEDNA
HHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCHHH
AARSLATRDLGSYTVASGDTLYSIARRSNLSIDDLRTLNQLDGNLVQVGQKLKLKADGAA
HHHHHHHHCCCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHCCHHHHHCCCEEEEECCCC
DNLVASNGGDGADPALVKVSETRPAASHARRSKEYVVQRGDTLFSIARRFGVTHNDIQRI
CCCEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHC
NGSRHPSHLQPGQKVKIVGL
CCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MKRFTPLALAVSLALPFASSVLAGAAPSSGVDEAMAAGLDMMLLNSSLLRNGDDVWKRAR
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHCCHHHHHHHH
EGFQLDEVNADTVRRQERFYASRPEYFKRTLDRSRKYLFHIMNEVERRGMPTEIALLPMV
CCCEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHH
ESAFVPTANSRVGAAGLWQFMPATGRHYGLEQTWWYDGRRDVTDATRAALDYLQNLYAQF
HHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
GDWNLALAAYNWGEGNLARAIAKAQASGIEPTYENIRLPNETRNYVPKLLAVRNILLNPE
CCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCH
KFGVHLDKFPNKPYFVAVSTGKHMNIDIAAKLAGISVAEFKELNPAFNLPVFAYKAGRQM
HHCEEHHCCCCCCEEEEEECCCEEEEEEEHHHHCCCHHHHHHCCCCCCCCEEEEECCCEE
LLPASKVDKFQANLDKWSKPLLTWEVYMPKSDESVGAVAGDHGMSAGQLLAANRISGGTL
ECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEECCCCCCCCCEEEECCCCCCCC
KAGQPVLVAMNKPLNDGQSFESVDTPIGDAPANAGATMIAQAEPAPISAPAAQPAMTQLA
CCCCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHH
LAATAQTTAPAQNNAATASPAAIVASAQPQVAPQPAADAGLAARQAVLLASSASSAEDNA
HHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCHHH
AARSLATRDLGSYTVASGDTLYSIARRSNLSIDDLRTLNQLDGNLVQVGQKLKLKADGAA
HHHHHHHHCCCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHCCHHHHHCCCEEEEECCCC
DNLVASNGGDGADPALVKVSETRPAASHARRSKEYVVQRGDTLFSIARRFGVTHNDIQRI
CCCEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHC
NGSRHPSHLQPGQKVKIVGL
CCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]