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The map label for this gene is prfB [H]

Identifier: 34496516

GI number: 34496516

Start: 1108512

End: 1109411

Strand: Reverse

Name: prfB [H]

Synonym: CV_1061

Alternate gene names: 34496516

Gene position: 1109411-1108512 (Counterclockwise)

Preceding gene: 34496517

Following gene: 34496515

Centisome position: 23.35

GC content: 64.67

Gene sequence:

>900_bases
GTGCTGGTGCTGGACGGCATCAACGCCACCATCGGCGACAGCCGCGAACTGTTCGACATGGGCAAGAGCGAGGAAGACGA
CGACACCATCCTCGCGGTGCAGGCCGATCTGGAAGAAGTGGAAGCCAAAGTGGCGCAGCTGGAATTCCGCCGCATGTTCC
ACGATCCGATGGACCCCACCAACTGCTTCATCGACATCCAGGCCGGCGCCGGCGGCACCGAAGCCCAGGACTGGGCCGGC
ATGCTGCTGCGCATGTATACCCGCTACGCCGAGCGCAAGGGCTTCAAGGTGGACGTGCTGGAACTGTCCGAGGGCGAAGT
GGCCGGCGTCACCAGCGCCACGCTGAAGATCGAGGGCGAGTACGCCTACGGCCTGCTGCGCACCGAAGTGGGCGTGCACC
GCCTGGTGCGCGTGTCGCCGTTCGACTCCAACGCCCGCCGCCACACCTCGTTCTCGTCGGTGTTCGTGTATCCGGAAGTG
GACGACAGCTTCGAGATCGACATCAACCCGGCGGACGTGCGCACCGACACCTATCGCGCCTCCGGCGCCGGCGGCCAGCA
CATCAACAAGACCGACTCCGCCGTGCGTCTGACCCACATCCCCACCGGCATCGTCGTGCAGTGCCAGAACGACCGTTCCC
AGCACCGCAACCGCGACGAGGCCTGGCAGATGCTGCGCGCCAAGCTGTACGAGCTGGAGCTGAAGAAGCGCAACGAAGCC
AAGCAGGCGCTGGAAGACACCAAGACCGACGTGGGCTGGGGCCACCAGATCCGCTCCTACGTGTTCGACCAATCCCGCAT
CAAGGATTTGCGCACCAGCTACGAAGTGGGCAACATCAAGGGCGTCATGGACGGCGACCTGGACGGCTTCATCGAAGCCA
GCCTCAAGCACGGCGTCTAA

Upstream 100 bases:

>100_bases
TCTGGAAGAAGTTAGCCGTCTGACCGAAGACCCGGATATCTGGAACGAGCCGAAAAAGGCGCAGGAGCTGGGCCGCGAGC
GCAAGCAGCTGGAAGACGTG

Downstream 100 bases:

>100_bases
GCCATATCGGGCCGGCTCCCATCGGGGCCGGCCTTCGCGTTGTACTTTTAGTTAATTGCCGAAGGAACCCTACCCGGCAC
GGAGTCATCATGTCTGATCA

Product: peptide chain release factor RF-2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLVLDGINATIGDSRELFDMGKSEEDDDTILAVQADLEEVEAKVAQLEFRRMFHDPMDPTNCFIDIQAGAGGTEAQDWAG
MLLRMYTRYAERKGFKVDVLELSEGEVAGVTSATLKIEGEYAYGLLRTEVGVHRLVRVSPFDSNARRHTSFSSVFVYPEV
DDSFEIDINPADVRTDTYRASGAGGQHINKTDSAVRLTHIPTGIVVQCQNDRSQHRNRDEAWQMLRAKLYELELKKRNEA
KQALEDTKTDVGWGHQIRSYVFDQSRIKDLRTSYEVGNIKGVMDGDLDGFIEASLKHGV

Sequences:

>Translated_299_residues
MLVLDGINATIGDSRELFDMGKSEEDDDTILAVQADLEEVEAKVAQLEFRRMFHDPMDPTNCFIDIQAGAGGTEAQDWAG
MLLRMYTRYAERKGFKVDVLELSEGEVAGVTSATLKIEGEYAYGLLRTEVGVHRLVRVSPFDSNARRHTSFSSVFVYPEV
DDSFEIDINPADVRTDTYRASGAGGQHINKTDSAVRLTHIPTGIVVQCQNDRSQHRNRDEAWQMLRAKLYELELKKRNEA
KQALEDTKTDVGWGHQIRSYVFDQSRIKDLRTSYEVGNIKGVMDGDLDGFIEASLKHGV
>Mature_299_residues
MLVLDGINATIGDSRELFDMGKSEEDDDTILAVQADLEEVEAKVAQLEFRRMFHDPMDPTNCFIDIQAGAGGTEAQDWAG
MLLRMYTRYAERKGFKVDVLELSEGEVAGVTSATLKIEGEYAYGLLRTEVGVHRLVRVSPFDSNARRHTSFSSVFVYPEV
DDSFEIDINPADVRTDTYRASGAGGQHINKTDSAVRLTHIPTGIVVQCQNDRSQHRNRDEAWQMLRAKLYELELKKRNEA
KQALEDTKTDVGWGHQIRSYVFDQSRIKDLRTSYEVGNIKGVMDGDLDGFIEASLKHGV

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=272, Percent_Identity=36.7647058823529, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI34577120, Length=299, Percent_Identity=35.7859531772575, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI166795305, Length=175, Percent_Identity=37.7142857142857, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI2367172, Length=297, Percent_Identity=62.962962962963, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1787462, Length=245, Percent_Identity=44.8979591836735, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=280, Percent_Identity=33.5714285714286, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321295, Length=271, Percent_Identity=36.5313653136531, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI19921226, Length=238, Percent_Identity=39.0756302521008, Blast_Score=155, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 33599; Mature: 33599

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLDGINATIGDSRELFDMGKSEEDDDTILAVQADLEEVEAKVAQLEFRRMFHDPMDPT
CEEECCCCCCCCCCHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCC
NCFIDIQAGAGGTEAQDWAGMLLRMYTRYAERKGFKVDVLELSEGEVAGVTSATLKIEGE
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEECEEEEEEEEECC
YAYGLLRTEVGVHRLVRVSPFDSNARRHTSFSSVFVYPEVDDSFEIDINPADVRTDTYRA
EEEEHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECCHHHCCCCCEEC
SGAGGQHINKTDSAVRLTHIPTGIVVQCQNDRSQHRNRDEAWQMLRAKLYELELKKRNEA
CCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
KQALEDTKTDVGWGHQIRSYVFDQSRIKDLRTSYEVGNIKGVMDGDLDGFIEASLKHGV
HHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHCCC
>Mature Secondary Structure
MLVLDGINATIGDSRELFDMGKSEEDDDTILAVQADLEEVEAKVAQLEFRRMFHDPMDPT
CEEECCCCCCCCCCHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCC
NCFIDIQAGAGGTEAQDWAGMLLRMYTRYAERKGFKVDVLELSEGEVAGVTSATLKIEGE
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEECEEEEEEEEECC
YAYGLLRTEVGVHRLVRVSPFDSNARRHTSFSSVFVYPEVDDSFEIDINPADVRTDTYRA
EEEEHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECCHHHCCCCCEEC
SGAGGQHINKTDSAVRLTHIPTGIVVQCQNDRSQHRNRDEAWQMLRAKLYELELKKRNEA
CCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
KQALEDTKTDVGWGHQIRSYVFDQSRIKDLRTSYEVGNIKGVMDGDLDGFIEASLKHGV
HHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA