Click here to switch to the map view.

The map label for this gene is lysS

Identifier: 34496515

GI number: 34496515

Start: 1106914

End: 1108422

Strand: Reverse

Name: lysS

Synonym: CV_1060

Alternate gene names: 34496515

Gene position: 1108422-1106914 (Counterclockwise)

Preceding gene: 34496516

Following gene: 34496514

Centisome position: 23.33

GC content: 62.89

Gene sequence:

>1509_bases
ATGTCTGATCACGAACAAGCACAGGCGCAAAGCCAAGACGAAAACCAGATCATGGCGGAACGCCGCCAGAAACTGCAGGC
GATCCGCGAAAAAGGCATCGCCTACCCCAACGACTTCAAACGCAGCCACTTCGCCAAACCGCTGCAGGACGACCACGCCG
CCAAAGAGGCAGAGGCGCTGGAAGCCGAGAAAATCGAAGTCGCCGTCGCCGGCCGCATGATGCTTAAACGTGTGATGGGC
AAAGCCAGCTTCGCCACCCTGCAGGACGTCAGCGGCCGCATCCAGGCCTTCATCTCCCGCGACAACGTGGGCGAAGACGT
CTACGCCGACTTCAAGCGCTGGGACCTGGGCGACATCGTCGCCGTCAAGGGCATGCTGTTCAAGACCAAGACCGGCGAAC
TGACGGTGCAGGCGACCGAAGTGCGCATGCTGTCCAAGAACATCCGTCCGCTGCCGGAGAAATTCCACGGCATCGCCGAC
CAGGAAACAAAGTACCGCCAGCGCTACGCCGACCTGATCATGAGCGAGGAAAGCCGCGAAACCTTCATCAAGCGCTCCAA
GATCGTGCAGAAGGTGCGCGACGTGATGGTGGGCGAAGGCTACCTGGAAGTGGAAACCCCGATGATGCACCCGATCCCGG
GCGGCGCGTCGGCCAAACCCTTCGTCACCCACCACAACGCGCTGGACATGCCGCTCTACCTGCGCATCGCCCCGGAGCTG
TACCTGAAGCGCCTGGTGGTCGGCGGCCTGGAACGCGTGTTCGAGATCAACCGCAACTTCCGCAACGAGGGGATGAGCAC
GCGCCACAACCCCGAGTTCACCATGATCGAGTTCTACGAAGCATATAGCGACTACCAGCGCATGATGGAGATGACCGAAA
CCATCATCCGCGAGTGCGCGCTGGTTGCCTGCGGCTCCACCACCGTGACCTACCAAGGCAAGGAAGTGGACCTGGGCAAG
CCGTTCGACCGCTTCACCATCGTGCAGGCGATCAAGCACTACAACCCGCAATACACCGACGCGCAACTGTCCGACGCCGC
CTGGGTGGCCAGCGAGATCAAGCGCCTGGGCGGCAAGCTGCCCCCGGCCCCGGGCCTGGGCAGCCTGCAGCTGGCGCTGT
TCGAGGAATGCGCCGAATCGCTGCTGTGGAACCCGACCTTCATCATCGACTACCCGGTGGAAGTGTCCCCGCTGGCGCGC
GGCTCCGACACCCAGCCGGGCCTGACCGAGCGTTTCGAACTGTTCATCGTCGGCCGCGAGCACGCCAACGGCTACTCGGA
GTTGAACGATCCGGAAGACCAGGCCGCGCGCTTCCAGGCGCAAGTCGCCCAGAAGGACGCCGGCGACGACGAGGCGATGC
ACTACGACGCCGACTACATCCGCGCGATGGAATACGGCCTGCCGCCGACCGGCGGTTGCGGCATCGGCATCGACCGCTTG
GTGATGTTGTTGACGGATGCGCCTTCTATCCGGGATGTGATCTTGTTCCCGCATATGCGTCCGGAGTAA

Upstream 100 bases:

>100_bases
ACGGCGTCTAAGCCATATCGGGCCGGCTCCCATCGGGGCCGGCCTTCGCGTTGTACTTTTAGTTAATTGCCGAAGGAACC
CTACCCGGCACGGAGTCATC

Downstream 100 bases:

>100_bases
GCTGTAGCAAGCTTACATTGGGCTAAAGAGAAAGCCGCTCACTGCAAGGTGAGCGGCTTTTTTGCGACCTTTAAACGTAG
CCTGTCCTATTTATCCATCA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSDHEQAQAQSQDENQIMAERRQKLQAIREKGIAYPNDFKRSHFAKPLQDDHAAKEAEALEAEKIEVAVAGRMMLKRVMG
KASFATLQDVSGRIQAFISRDNVGEDVYADFKRWDLGDIVAVKGMLFKTKTGELTVQATEVRMLSKNIRPLPEKFHGIAD
QETKYRQRYADLIMSEESRETFIKRSKIVQKVRDVMVGEGYLEVETPMMHPIPGGASAKPFVTHHNALDMPLYLRIAPEL
YLKRLVVGGLERVFEINRNFRNEGMSTRHNPEFTMIEFYEAYSDYQRMMEMTETIIRECALVACGSTTVTYQGKEVDLGK
PFDRFTIVQAIKHYNPQYTDAQLSDAAWVASEIKRLGGKLPPAPGLGSLQLALFEECAESLLWNPTFIIDYPVEVSPLAR
GSDTQPGLTERFELFIVGREHANGYSELNDPEDQAARFQAQVAQKDAGDDEAMHYDADYIRAMEYGLPPTGGCGIGIDRL
VMLLTDAPSIRDVILFPHMRPE

Sequences:

>Translated_502_residues
MSDHEQAQAQSQDENQIMAERRQKLQAIREKGIAYPNDFKRSHFAKPLQDDHAAKEAEALEAEKIEVAVAGRMMLKRVMG
KASFATLQDVSGRIQAFISRDNVGEDVYADFKRWDLGDIVAVKGMLFKTKTGELTVQATEVRMLSKNIRPLPEKFHGIAD
QETKYRQRYADLIMSEESRETFIKRSKIVQKVRDVMVGEGYLEVETPMMHPIPGGASAKPFVTHHNALDMPLYLRIAPEL
YLKRLVVGGLERVFEINRNFRNEGMSTRHNPEFTMIEFYEAYSDYQRMMEMTETIIRECALVACGSTTVTYQGKEVDLGK
PFDRFTIVQAIKHYNPQYTDAQLSDAAWVASEIKRLGGKLPPAPGLGSLQLALFEECAESLLWNPTFIIDYPVEVSPLAR
GSDTQPGLTERFELFIVGREHANGYSELNDPEDQAARFQAQVAQKDAGDDEAMHYDADYIRAMEYGLPPTGGCGIGIDRL
VMLLTDAPSIRDVILFPHMRPE
>Mature_501_residues
SDHEQAQAQSQDENQIMAERRQKLQAIREKGIAYPNDFKRSHFAKPLQDDHAAKEAEALEAEKIEVAVAGRMMLKRVMGK
ASFATLQDVSGRIQAFISRDNVGEDVYADFKRWDLGDIVAVKGMLFKTKTGELTVQATEVRMLSKNIRPLPEKFHGIADQ
ETKYRQRYADLIMSEESRETFIKRSKIVQKVRDVMVGEGYLEVETPMMHPIPGGASAKPFVTHHNALDMPLYLRIAPELY
LKRLVVGGLERVFEINRNFRNEGMSTRHNPEFTMIEFYEAYSDYQRMMEMTETIIRECALVACGSTTVTYQGKEVDLGKP
FDRFTIVQAIKHYNPQYTDAQLSDAAWVASEIKRLGGKLPPAPGLGSLQLALFEECAESLLWNPTFIIDYPVEVSPLARG
SDTQPGLTERFELFIVGREHANGYSELNDPEDQAARFQAQVAQKDAGDDEAMHYDADYIRAMEYGLPPTGGCGIGIDRLV
MLLTDAPSIRDVILFPHMRPE

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=519, Percent_Identity=42.0038535645472, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI194272210, Length=519, Percent_Identity=42.0038535645472, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI40789249, Length=244, Percent_Identity=25, Blast_Score=76, Evalue=9e-14,
Organism=Escherichia coli, GI1790571, Length=504, Percent_Identity=57.7380952380952, Blast_Score=616, Evalue=1e-177,
Organism=Escherichia coli, GI1789256, Length=499, Percent_Identity=59.1182364729459, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI87082379, Length=331, Percent_Identity=29.0030211480363, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1788173, Length=295, Percent_Identity=25.4237288135593, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=462, Percent_Identity=43.0735930735931, Blast_Score=349, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI17535927, Length=462, Percent_Identity=43.0735930735931, Blast_Score=348, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI71994340, Length=462, Percent_Identity=43.0735930735931, Blast_Score=347, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI32566633, Length=243, Percent_Identity=27.9835390946502, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=515, Percent_Identity=42.1359223300971, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324256, Length=391, Percent_Identity=32.7365728900256, Blast_Score=166, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24640849, Length=488, Percent_Identity=43.2377049180328, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24640851, Length=488, Percent_Identity=43.2377049180328, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI17136276, Length=471, Percent_Identity=23.5668789808917, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24584738, Length=367, Percent_Identity=24.7956403269755, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_CHRVO (Q7NZ62)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_900730.1
- ProteinModelPortal:   Q7NZ62
- SMR:   Q7NZ62
- GeneID:   2548098
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1060
- NMPDR:   fig|243365.1.peg.1060
- HOGENOM:   HBG631383
- OMA:   RNEGMDH
- PhylomeDB:   Q7NZ62
- ProtClustDB:   PRK00484
- BioCyc:   CVIO243365:CV_1060-MONOMER
- BRENDA:   6.1.1.6
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 56770; Mature: 56639

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHEQAQAQSQDENQIMAERRQKLQAIREKGIAYPNDFKRSHFAKPLQDDHAAKEAEAL
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHH
EAEKIEVAVAGRMMLKRVMGKASFATLQDVSGRIQAFISRDNVGEDVYADFKRWDLGDIV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHH
AVKGMLFKTKTGELTVQATEVRMLSKNIRPLPEKFHGIADQETKYRQRYADLIMSEESRE
HHHCEEEEECCCCEEEEHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCHHHH
TFIKRSKIVQKVRDVMVGEGYLEVETPMMHPIPGGASAKPFVTHHNALDMPLYLRIAPEL
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECHHH
YLKRLVVGGLERVFEINRNFRNEGMSTRHNPEFTMIEFYEAYSDYQRMMEMTETIIRECA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LVACGSTTVTYQGKEVDLGKPFDRFTIVQAIKHYNPQYTDAQLSDAAWVASEIKRLGGKL
HHEECCEEEEECCCEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
PPAPGLGSLQLALFEECAESLLWNPTFIIDYPVEVSPLARGSDTQPGLTERFELFIVGRE
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEECCCCCCCCCCCCCCCEEEEEEEECC
HANGYSELNDPEDQAARFQAQVAQKDAGDDEAMHYDADYIRAMEYGLPPTGGCGIGIDRL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHCCCCCCCCCCCHHHH
VMLLTDAPSIRDVILFPHMRPE
HHHHHCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
SDHEQAQAQSQDENQIMAERRQKLQAIREKGIAYPNDFKRSHFAKPLQDDHAAKEAEAL
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHH
EAEKIEVAVAGRMMLKRVMGKASFATLQDVSGRIQAFISRDNVGEDVYADFKRWDLGDIV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHH
AVKGMLFKTKTGELTVQATEVRMLSKNIRPLPEKFHGIADQETKYRQRYADLIMSEESRE
HHHCEEEEECCCCEEEEHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCHHHH
TFIKRSKIVQKVRDVMVGEGYLEVETPMMHPIPGGASAKPFVTHHNALDMPLYLRIAPEL
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECHHH
YLKRLVVGGLERVFEINRNFRNEGMSTRHNPEFTMIEFYEAYSDYQRMMEMTETIIRECA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LVACGSTTVTYQGKEVDLGKPFDRFTIVQAIKHYNPQYTDAQLSDAAWVASEIKRLGGKL
HHEECCEEEEECCCEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
PPAPGLGSLQLALFEECAESLLWNPTFIIDYPVEVSPLARGSDTQPGLTERFELFIVGRE
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEECCCCCCCCCCCCCCCEEEEEEEECC
HANGYSELNDPEDQAARFQAQVAQKDAGDDEAMHYDADYIRAMEYGLPPTGGCGIGIDRL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHCCCCCCCCCCCHHHH
VMLLTDAPSIRDVILFPHMRPE
HHHHHCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782