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The map label for this gene is mdh

Identifier: 34496517

GI number: 34496517

Start: 1109731

End: 1110711

Strand: Reverse

Name: mdh

Synonym: CV_1062

Alternate gene names: 34496517

Gene position: 1110711-1109731 (Counterclockwise)

Preceding gene: 34496532

Following gene: 34496516

Centisome position: 23.38

GC content: 64.53

Gene sequence:

>981_bases
ATGAAAGCCCCCGTTCGCGTTGCCGTTACCGGCGCTGCCGGCCAGATCGGCTACAGCCTGCTGTTCCGCATTGCCAGTGG
TGAAATGCTGGGCAAAGATCAACCCGTCATTCTTCATCTGCTGGACCTGCCGCAGGCTCAAACCGCGCTGAAGGGCGTGA
TGATGGAGCTGGAAGACTGTGCCTTCCCGCTGCTGGCCGGCATGGTCGCCACCGACGACCCGAACGTGGCATTCAAGGAT
GTGAAGGTCGCCCTGCTGGTCGGCGCCCGCCCGCGCAGCAAGGGCATGGAGCGCAAGGACCTGCTGGAAGCCAACGGCGC
CATCTTCACCGTTCAGGGCAAGGCTCTGAACGACCACGCCGCCCGCGACGTCAAGGTGCTGGTGGTGGGCAACCCGGCCA
ACACCAACGCCTGGATCGCGATGAAGTCCGCTCCGGACCTGGATCCGAAAAACTTCACCGCCATGTTGCGTCTTGACCAC
AACCGCGCGCTGTCGCAAATCGCCGCCAAGACCGGCAAGCCGGTGGCCTCCATCGAGAAGCTGGCCGTGTGGGGCAACCA
CTCGCCGACCATGTACGCCGACTACCGCTTCGCCACCATCGACGGCCAGTCGGTGAAGGCCATGATCAACGACGACGTGT
GGAACCGCGACGTGTTCCTGCCCACCGTGGGCAAGCGCGGCGCCGCCATCATCGAAGCGCGCGGCCTGTCCTCCGCCGCC
TCCGCCGCCAACGCCGCCATCGACCACATCCGCGACTGGGTGCTGGGCACCAATGGCAAGTGGGTGACCATGGGCGTGCC
CTCCGACGGTTCCTACGGCATCCCGGAAGGCGTGATGTACGGCGTACCGGTTGTATGCGAAAATGGCGAATACAAACGTG
TAGAAGGCCTGGAGATCGACGCGTTCAGCCGCGAGCGGATGGACCTGACCCTGGCCGAGCTGGAAGAAGAGCGCGCAGCC
ATCGCCCACCTGTTTGGCTGA

Upstream 100 bases:

>100_bases
AATCCAAATTCACGGGCAACCGTGTTACACTCGCAACACACCTATACTGCAGAGCAACATCTGCAGCTCATTATCGGATA
CATCAAAGGAGAGTCCGCCA

Downstream 100 bases:

>100_bases
TTGCCGGCCTGACCGAAGAGAAGCGCACCAAACGGTGCGCTTTTTGTCTTTGGTCAAACCGCGAATGAACATTTGAATTT
ACTAATACAGACTGCCATTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKAPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILHLLDLPQAQTALKGVMMELEDCAFPLLAGMVATDDPNVAFKD
VKVALLVGARPRSKGMERKDLLEANGAIFTVQGKALNDHAARDVKVLVVGNPANTNAWIAMKSAPDLDPKNFTAMLRLDH
NRALSQIAAKTGKPVASIEKLAVWGNHSPTMYADYRFATIDGQSVKAMINDDVWNRDVFLPTVGKRGAAIIEARGLSSAA
SAANAAIDHIRDWVLGTNGKWVTMGVPSDGSYGIPEGVMYGVPVVCENGEYKRVEGLEIDAFSRERMDLTLAELEEERAA
IAHLFG

Sequences:

>Translated_326_residues
MKAPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILHLLDLPQAQTALKGVMMELEDCAFPLLAGMVATDDPNVAFKD
VKVALLVGARPRSKGMERKDLLEANGAIFTVQGKALNDHAARDVKVLVVGNPANTNAWIAMKSAPDLDPKNFTAMLRLDH
NRALSQIAAKTGKPVASIEKLAVWGNHSPTMYADYRFATIDGQSVKAMINDDVWNRDVFLPTVGKRGAAIIEARGLSSAA
SAANAAIDHIRDWVLGTNGKWVTMGVPSDGSYGIPEGVMYGVPVVCENGEYKRVEGLEIDAFSRERMDLTLAELEEERAA
IAHLFG
>Mature_326_residues
MKAPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILHLLDLPQAQTALKGVMMELEDCAFPLLAGMVATDDPNVAFKD
VKVALLVGARPRSKGMERKDLLEANGAIFTVQGKALNDHAARDVKVLVVGNPANTNAWIAMKSAPDLDPKNFTAMLRLDH
NRALSQIAAKTGKPVASIEKLAVWGNHSPTMYADYRFATIDGQSVKAMINDDVWNRDVFLPTVGKRGAAIIEARGLSSAA
SAANAAIDHIRDWVLGTNGKWVTMGVPSDGSYGIPEGVMYGVPVVCENGEYKRVEGLEIDAFSRERMDLTLAELEEERAA
IAHLFG

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=326, Percent_Identity=53.9877300613497, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI89886456, Length=335, Percent_Identity=24.1791044776119, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17561064, Length=324, Percent_Identity=54.0123456790123, Blast_Score=326, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI32566798, Length=262, Percent_Identity=54.5801526717557, Blast_Score=260, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI32566800, Length=169, Percent_Identity=49.112426035503, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24583394, Length=323, Percent_Identity=54.1795665634675, Blast_Score=327, Evalue=8e-90,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_CHRVO (Q7NZ60)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_900732.1
- ProteinModelPortal:   Q7NZ60
- SMR:   Q7NZ60
- GeneID:   2547587
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_1062
- NMPDR:   fig|243365.1.peg.1062
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- BioCyc:   CVIO243365:CV_1062-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 35038; Mature: 35038

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 187-187 BINDING 92-92 BINDING 98-98 BINDING 105-105 BINDING 112-112 BINDING 131-131 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILHLLDLPQAQTALKGVMMELEDC
CCCCEEEEEECCCCHHHHHHHEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
AFPLLAGMVATDDPNVAFKDVKVALLVGARPRSKGMERKDLLEANGAIFTVQGKALNDHA
HHHHHHCEEECCCCCEEEEEEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCC
ARDVKVLVVGNPANTNAWIAMKSAPDLDPKNFTAMLRLDHNRALSQIAAKTGKPVASIEK
CCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCHHHHEE
LAVWGNHSPTMYADYRFATIDGQSVKAMINDDVWNRDVFLPTVGKRGAAIIEARGLSSAA
EEEECCCCCCEEEEEEEEEECCCEEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCHHH
SAANAAIDHIRDWVLGTNGKWVTMGVPSDGSYGIPEGVMYGVPVVCENGEYKRVEGLEID
HHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCCCCCEECCCEEECCCCEEEECCEEEE
AFSRERMDLTLAELEEERAAIAHLFG
CCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKAPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILHLLDLPQAQTALKGVMMELEDC
CCCCEEEEEECCCCHHHHHHHEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
AFPLLAGMVATDDPNVAFKDVKVALLVGARPRSKGMERKDLLEANGAIFTVQGKALNDHA
HHHHHHCEEECCCCCEEEEEEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCC
ARDVKVLVVGNPANTNAWIAMKSAPDLDPKNFTAMLRLDHNRALSQIAAKTGKPVASIEK
CCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCHHHHEE
LAVWGNHSPTMYADYRFATIDGQSVKAMINDDVWNRDVFLPTVGKRGAAIIEARGLSSAA
EEEECCCCCCEEEEEEEEEECCCEEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCHHH
SAANAAIDHIRDWVLGTNGKWVTMGVPSDGSYGIPEGVMYGVPVVCENGEYKRVEGLEID
HHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCCCCCEECCCEEECCCCEEEECCEEEE
AFSRERMDLTLAELEEERAAIAHLFG
CCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782