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The map label for this gene is livK [H]

Identifier: 34495860

GI number: 34495860

Start: 426702

End: 427856

Strand: Reverse

Name: livK [H]

Synonym: CV_0405

Alternate gene names: 34495860

Gene position: 427856-426702 (Counterclockwise)

Preceding gene: 34495861

Following gene: 34495859

Centisome position: 9.01

GC content: 66.15

Gene sequence:

>1155_bases
ATGAAAACCCGACACAGTCTGCTGCTGTGCACCTTGGTGGCAGGCGTCGCCGCGTGCAACAAACCCGCCAGCCAGGCGCC
GCAACCGGAACAGGCCGCCGCCGGCTCCGCGGTGGTCAAGATCGGCACCGCCAACCCGCTGACCGGCCCCTTCGCCCACT
GGGGGCGCGACGCCGACAACGGCGTCAAGCTGGCAGTGCAGGAGGCCAACGCCGAGAAGCTGACGCTGGACGGCAAGCCG
GTCACCTTCGAAGTGGTGTCGGAAGACGATCAGGCCGATCCCAAGGTCGCCACCCAGGTGGCCCAGCGCATGGTGGACGC
CAAGGTCGCCGGCATCGTCGGCCACCTGACTTCCGGCGCCGCCATCCCCGCCTCCCGCATCTACGCCGACGCCGGCATCC
CTATGATCTCCGGCTCAGTCACCAGCCCGTCGTTCACCCAGCAGGGCTACCGCAACACCTTCCGCCTGATCGCCAACGAC
CTGCAGCAGGGCCAGGCGCTGGCCAAGTACGCGGTGGACAAGCTGGGGGCGAAGCGCGTCGCCGTCATCGACGACCGCAC
CACCTACGGCCAAGGCCTGGCCGACGACTTCGCCAAGTCCGCCGAACAGGCCGGCGCCAAGGTGGTGAAACGCGAATTCA
CCACCAACACCGCCACCGACTTCATGGCGGTGCTCACCTCGATCAAGGGCGAGAAGCCCGATCTGCTGTTCTACGGCGGC
ATGGACGCCCAAGCGGGTCCGATGGTCAAGCAGATGGCCAAGCTCGGCATCAAGGCCGCCTTCATGGGCGCCGACGGCGT
CAACACGCCGGAATTCGCCAAGTTGGGCGGCGACAACGCCGAAGGCAGCTACGCCTCCAGCGCCGGCGCGCCCAAGGAAA
AGCTGCCTGGCTACGGCGAATTCAGCCAGAAGTACAAGCAGCAGTTCCAGTCCGACATCCAGGCCTACGCGCCGTACACC
TACGACGCCGCCAAGGTGCTGATCGCCGCGATGAAGCGCGCCGGTTCCGCCGAGCCGGCCAAGTATCTGCCAGAAATCGC
CAAGACCGATTACCAGGGCGTCACCGGACCGGTGAAGTTCGACGCCAAGGGCGATATCCAGCAGGCGACGGTGTCGCTGT
ATCAACTGAAGCAAGGCAAGTGGGTGGGACTGTAA

Upstream 100 bases:

>100_bases
CGTTCGATTGACAAAAGCGGGATCGGCCGACACAGTAAGCTCACGCCATAGCACCGGCGCAACTATCAAAATAAACAAAG
AGCATCAGGAGAGATTCATC

Downstream 100 bases:

>100_bases
GCATCCCTGTCGAACGGCAATAAAAAAACCCGCCAGGCAACTGGCGGGTTTTTCATGGAAACGGGCAGGAAGTCGCGCGA
TTCGCGCGCCGGAATGCGCA

Product: amino acid ABC transporter

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: L-BP; LS-BP [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKTRHSLLLCTLVAGVAACNKPASQAPQPEQAAAGSAVVKIGTANPLTGPFAHWGRDADNGVKLAVQEANAEKLTLDGKP
VTFEVVSEDDQADPKVATQVAQRMVDAKVAGIVGHLTSGAAIPASRIYADAGIPMISGSVTSPSFTQQGYRNTFRLIAND
LQQGQALAKYAVDKLGAKRVAVIDDRTTYGQGLADDFAKSAEQAGAKVVKREFTTNTATDFMAVLTSIKGEKPDLLFYGG
MDAQAGPMVKQMAKLGIKAAFMGADGVNTPEFAKLGGDNAEGSYASSAGAPKEKLPGYGEFSQKYKQQFQSDIQAYAPYT
YDAAKVLIAAMKRAGSAEPAKYLPEIAKTDYQGVTGPVKFDAKGDIQQATVSLYQLKQGKWVGL

Sequences:

>Translated_384_residues
MKTRHSLLLCTLVAGVAACNKPASQAPQPEQAAAGSAVVKIGTANPLTGPFAHWGRDADNGVKLAVQEANAEKLTLDGKP
VTFEVVSEDDQADPKVATQVAQRMVDAKVAGIVGHLTSGAAIPASRIYADAGIPMISGSVTSPSFTQQGYRNTFRLIAND
LQQGQALAKYAVDKLGAKRVAVIDDRTTYGQGLADDFAKSAEQAGAKVVKREFTTNTATDFMAVLTSIKGEKPDLLFYGG
MDAQAGPMVKQMAKLGIKAAFMGADGVNTPEFAKLGGDNAEGSYASSAGAPKEKLPGYGEFSQKYKQQFQSDIQAYAPYT
YDAAKVLIAAMKRAGSAEPAKYLPEIAKTDYQGVTGPVKFDAKGDIQQATVSLYQLKQGKWVGL
>Mature_384_residues
MKTRHSLLLCTLVAGVAACNKPASQAPQPEQAAAGSAVVKIGTANPLTGPFAHWGRDADNGVKLAVQEANAEKLTLDGKP
VTFEVVSEDDQADPKVATQVAQRMVDAKVAGIVGHLTSGAAIPASRIYADAGIPMISGSVTSPSFTQQGYRNTFRLIAND
LQQGQALAKYAVDKLGAKRVAVIDDRTTYGQGLADDFAKSAEQAGAKVVKREFTTNTATDFMAVLTSIKGEKPDLLFYGG
MDAQAGPMVKQMAKLGIKAAFMGADGVNTPEFAKLGGDNAEGSYASSAGAPKEKLPGYGEFSQKYKQQFQSDIQAYAPYT
YDAAKVLIAAMKRAGSAEPAKYLPEIAKTDYQGVTGPVKFDAKGDIQQATVSLYQLKQGKWVGL

Specific function: This protein is a component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids in E.coli [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=339, Percent_Identity=33.0383480825959, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI48994941, Length=332, Percent_Identity=31.6265060240964, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40439; Mature: 40439

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRHSLLLCTLVAGVAACNKPASQAPQPEQAAAGSAVVKIGTANPLTGPFAHWGRDADN
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEEECCCCCCCCCHHHCCCCCCC
GVKLAVQEANAEKLTLDGKPVTFEVVSEDDQADPKVATQVAQRMVDAKVAGIVGHLTSGA
CEEEEEEECCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
AIPASRIYADAGIPMISGSVTSPSFTQQGYRNTFRLIANDLQQGQALAKYAVDKLGAKRV
CCCHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
AVIDDRTTYGQGLADDFAKSAEQAGAKVVKREFTTNTATDFMAVLTSIKGEKPDLLFYGG
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECC
MDAQAGPMVKQMAKLGIKAAFMGADGVNTPEFAKLGGDNAEGSYASSAGAPKEKLPGYGE
CCCCCCHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHCCCCCH
FSQKYKQQFQSDIQAYAPYTYDAAKVLIAAMKRAGSAEPAKYLPEIAKTDYQGVTGPVKF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEE
DAKGDIQQATVSLYQLKQGKWVGL
CCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKTRHSLLLCTLVAGVAACNKPASQAPQPEQAAAGSAVVKIGTANPLTGPFAHWGRDADN
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEEECCCCCCCCCHHHCCCCCCC
GVKLAVQEANAEKLTLDGKPVTFEVVSEDDQADPKVATQVAQRMVDAKVAGIVGHLTSGA
CEEEEEEECCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
AIPASRIYADAGIPMISGSVTSPSFTQQGYRNTFRLIANDLQQGQALAKYAVDKLGAKRV
CCCHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
AVIDDRTTYGQGLADDFAKSAEQAGAKVVKREFTTNTATDFMAVLTSIKGEKPDLLFYGG
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECC
MDAQAGPMVKQMAKLGIKAAFMGADGVNTPEFAKLGGDNAEGSYASSAGAPKEKLPGYGE
CCCCCCHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHCCCCCH
FSQKYKQQFQSDIQAYAPYTYDAAKVLIAAMKRAGSAEPAKYLPEIAKTDYQGVTGPVKF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEE
DAKGDIQQATVSLYQLKQGKWVGL
CCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3891753; 4077929; 2195019; 8041620; 9278503; 6990419; 9298646; 2649683 [H]