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The map label for this gene is clcA [H]

Identifier: 33864514

GI number: 33864514

Start: 2379935

End: 2381293

Strand: Direct

Name: clcA [H]

Synonym: PMT2250

Alternate gene names: 33864514

Gene position: 2379935-2381293 (Clockwise)

Preceding gene: 33864513

Following gene: 33864515

Centisome position: 98.72

GC content: 52.1

Gene sequence:

>1359_bases
ATGGCCATGCCGAATGATCTAAACCGGCAACAAAATCTGATCCAGTCCAGTCGCAGCATCCGCAAACTCTTAGAGCAGCG
CTGGTTTGTGGTTGTGCTCGCTCTGATGCTTACGGGCCTTGGAGCTGCCCTCACAGGAGTGCTTTTCAAAGCTGGTATCT
ATAGCCTCGCCAAGTGGCGATTCCAGCTTCTTGAACTTCTTCCGCCATGGATCGTTCTACCAGCCCTTGGTGCCACAGGA
GGCTTGGTTTCTGGGTTGCTTGTTTCCCGTCTAGCCCCCTCTGCGAGCGGTTCAGGGGTGAGCCACATCATGGCGTTTTT
ACGCCATCGATCCGTACCAATGGGCTTGAGGGTTGGGATGGTCAAGCTAGTCGCAGGAGTTATCGCCATCGGCAGTGGTT
TTCCACTTGGTCCAGAAGGACCAGCAGTGCAAATGGGAGGCTCTGTCGCTTGGAAATTTGCTCAGTGGTTAAAGGCACCA
ATCGCCTTTCGCCGAGTCATTGTCGCTGCTGGAGGTGGGGCAGGTCTAGCCGCTATTTTCAGTGCGCCAATTGGAGGCTT
TGTTTATGTAATTGAAGAGCTACTTCATTCTGCAAAACCTGCAGTCTTGCTGCTGGTGGTTGTCACCACCTTCTGGGCTG
ATACTTGGGCAGATGTGTTCGGATTTCTAGGACTAAGTCCATCAGCTGGAAGTCTTAGCAAAGGCGTGGGCTTTCAACTG
GAACGTGAATACACACCCCTAGTGCACTTTCTACCAATCGACCTGGGCTACCTAATCGCCCTTGGTTTGGTCACTGGAGT
CTTGGCGGAGTTTTACTGCCGCTACGTCCTAGCGATGCAACGCAAAGGTAAAACCTGGTTCGGCAATCGACTCGTTTTAA
GGATGGTGCTCAGTGGTGGTGTACTCGGAGGGGTTTATGCAGCTCTTCCTGAGAGCTTTAGGAATTTGGACAACCTCCAA
GATCTGGTCGGTGGAGGAGAAGCTGGTCCTCTGATGGCTCTCAGCACCTTCGTCGTGCTGTTTTTCACTACCGGGCTAGC
AGCTGCCTCAGGAGCACCAGGAGGCTTATTTGTTCCGATGCTGACCCTAGGAGGAGCAATCGGCCTGGCCTGTGGAGACT
GGGCACAAATGCTGACTGGCCATGCACCCAGTACCTTCATTTTTGCAGGGATGGGAGCGTTCGTAGCCAGCTGCTCACGC
ACACCGATCACGGCCATGTTTTTGGCCTTCGCACTAACAAAAGATTTGCTAATCCTCAAACCAATACTAGTGACATGCCT
AGCGAGCTTTCTCGTCGCTCGCCTCTTTAATGAACACTCAATTTATGAAAGACAAATCACAATGGAATGCAAAGAGTAA

Upstream 100 bases:

>100_bases
CGCTATCTAAATTTCAACCAGATTGATGGCTTTGCAGATCAAGGGCGCGTGGTAAATAAAGACATACAAGCAGAAGTTCT
TGCAGAGTTTTCACCCACCA

Downstream 100 bases:

>100_bases
CCAATCGAAAAAAGTAATCTACTACTTATAATATCTAAATAAACTGGACAATTTGCAGACAGCCCATATTATTTGAATTC
TCATAATCTCCATCATAAGA

Product: chloride channel

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MAMPNDLNRQQNLIQSSRSIRKLLEQRWFVVVLALMLTGLGAALTGVLFKAGIYSLAKWRFQLLELLPPWIVLPALGATG
GLVSGLLVSRLAPSASGSGVSHIMAFLRHRSVPMGLRVGMVKLVAGVIAIGSGFPLGPEGPAVQMGGSVAWKFAQWLKAP
IAFRRVIVAAGGGAGLAAIFSAPIGGFVYVIEELLHSAKPAVLLLVVVTTFWADTWADVFGFLGLSPSAGSLSKGVGFQL
EREYTPLVHFLPIDLGYLIALGLVTGVLAEFYCRYVLAMQRKGKTWFGNRLVLRMVLSGGVLGGVYAALPESFRNLDNLQ
DLVGGGEAGPLMALSTFVVLFFTTGLAAASGAPGGLFVPMLTLGGAIGLACGDWAQMLTGHAPSTFIFAGMGAFVASCSR
TPITAMFLAFALTKDLLILKPILVTCLASFLVARLFNEHSIYERQITMECKE

Sequences:

>Translated_452_residues
MAMPNDLNRQQNLIQSSRSIRKLLEQRWFVVVLALMLTGLGAALTGVLFKAGIYSLAKWRFQLLELLPPWIVLPALGATG
GLVSGLLVSRLAPSASGSGVSHIMAFLRHRSVPMGLRVGMVKLVAGVIAIGSGFPLGPEGPAVQMGGSVAWKFAQWLKAP
IAFRRVIVAAGGGAGLAAIFSAPIGGFVYVIEELLHSAKPAVLLLVVVTTFWADTWADVFGFLGLSPSAGSLSKGVGFQL
EREYTPLVHFLPIDLGYLIALGLVTGVLAEFYCRYVLAMQRKGKTWFGNRLVLRMVLSGGVLGGVYAALPESFRNLDNLQ
DLVGGGEAGPLMALSTFVVLFFTTGLAAASGAPGGLFVPMLTLGGAIGLACGDWAQMLTGHAPSTFIFAGMGAFVASCSR
TPITAMFLAFALTKDLLILKPILVTCLASFLVARLFNEHSIYERQITMECKE
>Mature_451_residues
AMPNDLNRQQNLIQSSRSIRKLLEQRWFVVVLALMLTGLGAALTGVLFKAGIYSLAKWRFQLLELLPPWIVLPALGATGG
LVSGLLVSRLAPSASGSGVSHIMAFLRHRSVPMGLRVGMVKLVAGVIAIGSGFPLGPEGPAVQMGGSVAWKFAQWLKAPI
AFRRVIVAAGGGAGLAAIFSAPIGGFVYVIEELLHSAKPAVLLLVVVTTFWADTWADVFGFLGLSPSAGSLSKGVGFQLE
REYTPLVHFLPIDLGYLIALGLVTGVLAEFYCRYVLAMQRKGKTWFGNRLVLRMVLSGGVLGGVYAALPESFRNLDNLQD
LVGGGEAGPLMALSTFVVLFFTTGLAAASGAPGGLFVPMLTLGGAIGLACGDWAQMLTGHAPSTFIFAGMGAFVASCSRT
PITAMFLAFALTKDLLILKPILVTCLASFLVARLFNEHSIYERQITMECKE

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Homo sapiens, GI55770840, Length=430, Percent_Identity=24.4186046511628, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI55770838, Length=430, Percent_Identity=24.4186046511628, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1786350, Length=374, Percent_Identity=31.283422459893, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71985148, Length=441, Percent_Identity=24.7165532879819, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71985141, Length=441, Percent_Identity=24.7165532879819, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322500, Length=404, Percent_Identity=23.5148514851485, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 47919; Mature: 47787

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AMPNDLNRQQNLIQSSRSIRKLLEQRWFVVVLALMLTGLGAALTGVLFKAGIYSLAKWR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA