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The map label for this gene is acnB [H]

Identifier: 33864513

GI number: 33864513

Start: 2377327

End: 2379951

Strand: Direct

Name: acnB [H]

Synonym: PMT2249

Alternate gene names: 33864513

Gene position: 2377327-2379951 (Clockwise)

Preceding gene: 33864511

Following gene: 33864514

Centisome position: 98.61

GC content: 52.19

Gene sequence:

>2625_bases
ATGCTGAGTGCTTATCGCCAGCTCGCCGCCGAACGTGACGCCCAGGGCATCCCCCCCCTGCCCCTCACTGCCGAGCAAAC
CCAAGCACTCACCTTACTTTTACAGAATCCACCAGCAGGAGACAGCTCTGAACTGCTTTACCTGCTGCGTGAGCGCATCC
CCCCAGGAGTAGATGAAGCTGCCTACGTGAAAGCAACCTGGCTGAGTGCTATCGCCCAGGGAAACAGCAAGAGCCCACTC
ATTACTCCCTTAGAAGCCACAAAACTGCTAGGCACGATGATTGGGGGGTACAACGTTGCAGCTCTGATTGAGCTGCTTCA
ACACAATGACCACGAGCTAGCTGAATGCGCCGCTACAGGTCTGAGTCGCACCCTGCTCGTCTACGACGCTGTCCATGACG
TTATCGAGCTAGCTGAAACCAATCGCTTCGCCAAAAAAGTTGTAGACAGCTGGGCCGCCGCTGAATGGTTCACCTCAAAA
CCAATCTTAGCCAAAGAGATCACAGTCACAGTCTTCAAAGTTGATGGTGAAACCAATACCGACGATCTATCCCCCGCCAC
CCATGCCACCACCCGACCAGACATCCCCCTGCATGCTCTGGCCATGCTGGAAACACGGGATCCCAATGCCCTTGCCACGA
TCATCAGCCTGAAGGCAAAAGGCCATCCCATTGCCTACGTGGGCGATGTAGTGGGTACGGGCAGTTCTCGTAAATCAGCA
ATCAATTCGGTGCTTTGGCACATAGGAGCCGATATCCCTTACGTACCCAACAAGCGATCCGGCGGCGTCATCCTTGGCGG
CAAAATCGCTCCCATTTTCTTCAACACAGCGGAAGACTCCGGTGCCCTACCAATCGAATGTGATGTCAGTCAGCTGCAAA
GTGGCGATGTGATCACCATCCGTCCACATGCCGGCACCATCGAACGGGCTTTCGGAGAAGACAAGGCAGGCGAAATCGTG
GCTCACTTCAAGCTCAAACCAATCACCATCAGCGACGAAGTGCGCGCGGGTGGGCGTATCTCCCTACTGATCGGCAGAGC
ACTAACCGACAAAGTGCGTTCTCAATTTGGACTGTCAACATCCGATCTATTCGTCCGCCCAGGAGACACTCCTGAAACGG
GCAAGGGGTATACCCAGGCCCAAAAAATCGTTGGTCTTGCTTGTGGTCTCGAAGGAATACGACCTGGGACCAGTTGCGAA
CCGCTGATGACAACTGTAGGCAGCCAAGACACAACTGGACCAATGACTCGGGACGAAATGAAGGAGCTCGCCTGCCTGGG
ATTTGCAGCAGATCTGGTCATGCAAAGCTTCTGCCACACCGCTGCCTACCCGAAGCCTGTAGATCTACAAACCCAGAAAG
AACTTCCCGATTTCTTCGCCCAACGTGGTGGAGTAGCACTTCATCCAGGCGATGGGATCATCCACAGCTGGCTGAACCGC
ATGCTTCTGCCAGACACAGTGGGAACAGGCGGCGATAGCCACACTCGTTTCCCCTTAGGCATTTCTTTCCCCGGAGGTTC
TGGCGTGGTGGCTTTTGCCGCAGCCATCGGTGCCATGCCCCTAGACATGCCGGAATCTGTACTGGTTCGTTTCAGCGGCT
CACTACAGAGCGGCATCACACTGCGTGACGTCGTCAATGCGATCCCCTTGATCGCGATTCAAGAAGGTCTACTCAGCGTG
GAAAAAGCCAAAAAGCTCAATGTCTTTAGCGGCAAAATCATGGAAATCGAAGGATTACCTGATCTGAAGCTGGAGCAAGC
CTTTGAACTAACCGATGCCACTGCTGAACGTTCCTGCGCTGGCTGCACGATCAAACTGTCTGAATCAACGGTTGCTGAAT
ACCTTCGCAGCAATATCGCATTGCTAAAAAACATGATTGCCCGCGGTTATCAGGATGCTCGCACCCTGGCAAGACGCATC
AAAGCCATGGAAGCTTGGCTGGCAAAGCCAGAATTGATTAGCGCTGATCAAAACGCAGAGTATGCCGAAATCATCAATAT
CAATCTCGACGAACTCAAAGAACCCGTACTGGCCTGTCCCAACGATCCCGACAATGTAAAACTACTCAGTGAGGTTGCAA
ACAATCCAGTGCAGGAAGTCTTTATCGGCTCCTGTATGACCAACATCGGCCACTACAGAGCTGCGGCAAAAATTCTTGAA
GGGTCTGGAACCAGCAAAGCTCGCCTATGGGTTTGCCCACCAACAAGAATGGATGAAGCCATGCTTCAAGAAGAGGGTTA
CTACAAAATCTTCGAAGCGGCAGGGAGCCGAATGGAAATGCCTGGTTGTTCATTATGTATGGGCAATCAAGCACGCGTGG
AAGACAACACAACCGTTTTCTCCACAAGCACTCGAAACTTCAACAATCGACTAGGCCAAGGAGCCCAGGTCTATTTAGGC
AGTGCTGAGTTGGCAGCAGTATGTGCATTATTAGGCAGAATCCCTACACCAAAAGAATATCTTTCGATCGCAACAGAAAA
AATCGATCCCCTCTCATCTGAGCTTTATCGCTATCTAAATTTCAACCAGATTGATGGCTTTGCAGATCAAGGGCGCGTGG
TAAATAAAGACATACAAGCAGAAGTTCTTGCAGAGTTTTCACCCACCAATGGCCATGCCGAATGA

Upstream 100 bases:

>100_bases
CAGCATCCGGTAATTGCATTTCAATATCGATCTGCTTCCAGGATGGATCGACGGCAAATAACAATAGAAGGGCCATAACC
GCTGCCGAGGAATCACCCCA

Downstream 100 bases:

>100_bases
TCTAAACCGGCAACAAAATCTGATCCAGTCCAGTCGCAGCATCCGCAAACTCTTAGAGCAGCGCTGGTTTGTGGTTGTGC
TCGCTCTGATGCTTACGGGC

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 874; Mature: 874

Protein sequence:

>874_residues
MLSAYRQLAAERDAQGIPPLPLTAEQTQALTLLLQNPPAGDSSELLYLLRERIPPGVDEAAYVKATWLSAIAQGNSKSPL
ITPLEATKLLGTMIGGYNVAALIELLQHNDHELAECAATGLSRTLLVYDAVHDVIELAETNRFAKKVVDSWAAAEWFTSK
PILAKEITVTVFKVDGETNTDDLSPATHATTRPDIPLHALAMLETRDPNALATIISLKAKGHPIAYVGDVVGTGSSRKSA
INSVLWHIGADIPYVPNKRSGGVILGGKIAPIFFNTAEDSGALPIECDVSQLQSGDVITIRPHAGTIERAFGEDKAGEIV
AHFKLKPITISDEVRAGGRISLLIGRALTDKVRSQFGLSTSDLFVRPGDTPETGKGYTQAQKIVGLACGLEGIRPGTSCE
PLMTTVGSQDTTGPMTRDEMKELACLGFAADLVMQSFCHTAAYPKPVDLQTQKELPDFFAQRGGVALHPGDGIIHSWLNR
MLLPDTVGTGGDSHTRFPLGISFPGGSGVVAFAAAIGAMPLDMPESVLVRFSGSLQSGITLRDVVNAIPLIAIQEGLLSV
EKAKKLNVFSGKIMEIEGLPDLKLEQAFELTDATAERSCAGCTIKLSESTVAEYLRSNIALLKNMIARGYQDARTLARRI
KAMEAWLAKPELISADQNAEYAEIININLDELKEPVLACPNDPDNVKLLSEVANNPVQEVFIGSCMTNIGHYRAAAKILE
GSGTSKARLWVCPPTRMDEAMLQEEGYYKIFEAAGSRMEMPGCSLCMGNQARVEDNTTVFSTSTRNFNNRLGQGAQVYLG
SAELAAVCALLGRIPTPKEYLSIATEKIDPLSSELYRYLNFNQIDGFADQGRVVNKDIQAEVLAEFSPTNGHAE

Sequences:

>Translated_874_residues
MLSAYRQLAAERDAQGIPPLPLTAEQTQALTLLLQNPPAGDSSELLYLLRERIPPGVDEAAYVKATWLSAIAQGNSKSPL
ITPLEATKLLGTMIGGYNVAALIELLQHNDHELAECAATGLSRTLLVYDAVHDVIELAETNRFAKKVVDSWAAAEWFTSK
PILAKEITVTVFKVDGETNTDDLSPATHATTRPDIPLHALAMLETRDPNALATIISLKAKGHPIAYVGDVVGTGSSRKSA
INSVLWHIGADIPYVPNKRSGGVILGGKIAPIFFNTAEDSGALPIECDVSQLQSGDVITIRPHAGTIERAFGEDKAGEIV
AHFKLKPITISDEVRAGGRISLLIGRALTDKVRSQFGLSTSDLFVRPGDTPETGKGYTQAQKIVGLACGLEGIRPGTSCE
PLMTTVGSQDTTGPMTRDEMKELACLGFAADLVMQSFCHTAAYPKPVDLQTQKELPDFFAQRGGVALHPGDGIIHSWLNR
MLLPDTVGTGGDSHTRFPLGISFPGGSGVVAFAAAIGAMPLDMPESVLVRFSGSLQSGITLRDVVNAIPLIAIQEGLLSV
EKAKKLNVFSGKIMEIEGLPDLKLEQAFELTDATAERSCAGCTIKLSESTVAEYLRSNIALLKNMIARGYQDARTLARRI
KAMEAWLAKPELISADQNAEYAEIININLDELKEPVLACPNDPDNVKLLSEVANNPVQEVFIGSCMTNIGHYRAAAKILE
GSGTSKARLWVCPPTRMDEAMLQEEGYYKIFEAAGSRMEMPGCSLCMGNQARVEDNTTVFSTSTRNFNNRLGQGAQVYLG
SAELAAVCALLGRIPTPKEYLSIATEKIDPLSSELYRYLNFNQIDGFADQGRVVNKDIQAEVLAEFSPTNGHAE
>Mature_874_residues
MLSAYRQLAAERDAQGIPPLPLTAEQTQALTLLLQNPPAGDSSELLYLLRERIPPGVDEAAYVKATWLSAIAQGNSKSPL
ITPLEATKLLGTMIGGYNVAALIELLQHNDHELAECAATGLSRTLLVYDAVHDVIELAETNRFAKKVVDSWAAAEWFTSK
PILAKEITVTVFKVDGETNTDDLSPATHATTRPDIPLHALAMLETRDPNALATIISLKAKGHPIAYVGDVVGTGSSRKSA
INSVLWHIGADIPYVPNKRSGGVILGGKIAPIFFNTAEDSGALPIECDVSQLQSGDVITIRPHAGTIERAFGEDKAGEIV
AHFKLKPITISDEVRAGGRISLLIGRALTDKVRSQFGLSTSDLFVRPGDTPETGKGYTQAQKIVGLACGLEGIRPGTSCE
PLMTTVGSQDTTGPMTRDEMKELACLGFAADLVMQSFCHTAAYPKPVDLQTQKELPDFFAQRGGVALHPGDGIIHSWLNR
MLLPDTVGTGGDSHTRFPLGISFPGGSGVVAFAAAIGAMPLDMPESVLVRFSGSLQSGITLRDVVNAIPLIAIQEGLLSV
EKAKKLNVFSGKIMEIEGLPDLKLEQAFELTDATAERSCAGCTIKLSESTVAEYLRSNIALLKNMIARGYQDARTLARRI
KAMEAWLAKPELISADQNAEYAEIININLDELKEPVLACPNDPDNVKLLSEVANNPVQEVFIGSCMTNIGHYRAAAKILE
GSGTSKARLWVCPPTRMDEAMLQEEGYYKIFEAAGSRMEMPGCSLCMGNQARVEDNTTVFSTSTRNFNNRLGQGAQVYLG
SAELAAVCALLGRIPTPKEYLSIATEKIDPLSSELYRYLNFNQIDGFADQGRVVNKDIQAEVLAEFSPTNGHAE

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=862, Percent_Identity=63.9211136890951, Blast_Score=1077, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=369, Percent_Identity=26.8292682926829, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI32564738, Length=393, Percent_Identity=24.9363867684478, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=393, Percent_Identity=24.9363867684478, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322261, Length=408, Percent_Identity=24.5098039215686, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI281365315, Length=394, Percent_Identity=25.1269035532995, Blast_Score=75, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17864292, Length=394, Percent_Identity=25.1269035532995, Blast_Score=75, Evalue=3e-13,
Organism=Drosophila melanogaster, GI161076999, Length=394, Percent_Identity=25.1269035532995, Blast_Score=75, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 93996; Mature: 93996

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00450 ACONITASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAYRQLAAERDAQGIPPLPLTAEQTQALTLLLQNPPAGDSSELLYLLRERIPPGVDEA
CCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCH
AYVKATWLSAIAQGNSKSPLITPLEATKLLGTMIGGYNVAALIELLQHNDHELAECAATG
HHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHC
LSRTLLVYDAVHDVIELAETNRFAKKVVDSWAAAEWFTSKPILAKEITVTVFKVDGETNT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCC
DDLSPATHATTRPDIPLHALAMLETRDPNALATIISLKAKGHPIAYVGDVVGTGSSRKSA
CCCCCCCCCCCCCCCCHHHEEEEECCCCCCEEEEEEEECCCCCEEEECCCCCCCCHHHHH
INSVLWHIGADIPYVPNKRSGGVILGGKIAPIFFNTAEDSGALPIECDVSQLQSGDVITI
HHHHHHHHCCCCCCCCCCCCCCEEECCEEEEEEEECCCCCCCCEEEECHHHCCCCCEEEE
RPHAGTIERAFGEDKAGEIVAHFKLKPITISDEVRAGGRISLLIGRALTDKVRSQFGLST
CCCCCCHHHHCCCCCCCCEEEEEEEEEEEECHHHCCCCEEEEEECHHHHHHHHHHHCCCC
SDLFVRPGDTPETGKGYTQAQKIVGLACGLEGIRPGTSCEPLMTTVGSQDTTGPMTRDEM
CCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHH
KELACLGFAADLVMQSFCHTAAYPKPVDLQTQKELPDFFAQRGGVALHPGDGIIHSWLNR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHH
MLLPDTVGTGGDSHTRFPLGISFPGGSGVVAFAAAIGAMPLDMPESVLVRFSGSLQSGIT
HCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHEECCCHHCCCC
LRDVVNAIPLIAIQEGLLSVEKAKKLNVFSGKIMEIEGLPDLKLEQAFELTDATAERSCA
HHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCC
GCTIKLSESTVAEYLRSNIALLKNMIARGYQDARTLARRIKAMEAWLAKPELISADQNAE
CCEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
YAEIININLDELKEPVLACPNDPDNVKLLSEVANNPVQEVFIGSCMTNIGHYRAAAKILE
EEEEEECCHHHHCCCCEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
GSGTSKARLWVCPPTRMDEAMLQEEGYYKIFEAAGSRMEMPGCSLCMGNQARVEDNTTVF
CCCCCCEEEEECCCCCHHHHHHHCCCCCHHHHHCCCCCCCCCCCEECCCCCEECCCCEEE
STSTRNFNNRLGQGAQVYLGSAELAAVCALLGRIPTPKEYLSIATEKIDPLSSELYRYLN
EECCCCHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHC
FNQIDGFADQGRVVNKDIQAEVLAEFSPTNGHAE
CCCCCCCCCCCEEECCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLSAYRQLAAERDAQGIPPLPLTAEQTQALTLLLQNPPAGDSSELLYLLRERIPPGVDEA
CCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCH
AYVKATWLSAIAQGNSKSPLITPLEATKLLGTMIGGYNVAALIELLQHNDHELAECAATG
HHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHC
LSRTLLVYDAVHDVIELAETNRFAKKVVDSWAAAEWFTSKPILAKEITVTVFKVDGETNT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCC
DDLSPATHATTRPDIPLHALAMLETRDPNALATIISLKAKGHPIAYVGDVVGTGSSRKSA
CCCCCCCCCCCCCCCCHHHEEEEECCCCCCEEEEEEEECCCCCEEEECCCCCCCCHHHHH
INSVLWHIGADIPYVPNKRSGGVILGGKIAPIFFNTAEDSGALPIECDVSQLQSGDVITI
HHHHHHHHCCCCCCCCCCCCCCEEECCEEEEEEEECCCCCCCCEEEECHHHCCCCCEEEE
RPHAGTIERAFGEDKAGEIVAHFKLKPITISDEVRAGGRISLLIGRALTDKVRSQFGLST
CCCCCCHHHHCCCCCCCCEEEEEEEEEEEECHHHCCCCEEEEEECHHHHHHHHHHHCCCC
SDLFVRPGDTPETGKGYTQAQKIVGLACGLEGIRPGTSCEPLMTTVGSQDTTGPMTRDEM
CCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHH
KELACLGFAADLVMQSFCHTAAYPKPVDLQTQKELPDFFAQRGGVALHPGDGIIHSWLNR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHH
MLLPDTVGTGGDSHTRFPLGISFPGGSGVVAFAAAIGAMPLDMPESVLVRFSGSLQSGIT
HCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHEECCCHHCCCC
LRDVVNAIPLIAIQEGLLSVEKAKKLNVFSGKIMEIEGLPDLKLEQAFELTDATAERSCA
HHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCC
GCTIKLSESTVAEYLRSNIALLKNMIARGYQDARTLARRIKAMEAWLAKPELISADQNAE
CCEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
YAEIININLDELKEPVLACPNDPDNVKLLSEVANNPVQEVFIGSCMTNIGHYRAAAKILE
EEEEEECCHHHHCCCCEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
GSGTSKARLWVCPPTRMDEAMLQEEGYYKIFEAAGSRMEMPGCSLCMGNQARVEDNTTVF
CCCCCCEEEEECCCCCHHHHHHHCCCCCHHHHHCCCCCCCCCCCEECCCCCEECCCCEEE
STSTRNFNNRLGQGAQVYLGSAELAAVCALLGRIPTPKEYLSIATEKIDPLSSELYRYLN
EECCCCHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHC
FNQIDGFADQGRVVNKDIQAEVLAEFSPTNGHAE
CCCCCCCCCCCEEECCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]