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The map label for this gene is aspS [H]

Identifier: 33864480

GI number: 33864480

Start: 2342881

End: 2344701

Strand: Direct

Name: aspS [H]

Synonym: PMT2216

Alternate gene names: 33864480

Gene position: 2342881-2344701 (Clockwise)

Preceding gene: 33864479

Following gene: 33864481

Centisome position: 97.18

GC content: 55.85

Gene sequence:

>1821_bases
ATGCGCAGCAACGGTTGCGGCGAACTGCGCAGTAAGCACATCGCCTCCAACGTGAAACTGTGTGGCTGGGTTGATCGCTG
TCGAGACCATGGCGGAGTGATCTTCATCGATCTACGAGACCGCAGTGGAACCATACAAATCACCGTGGATCCCGATCAAG
GTACTGAGCTCTTCGCAAGCGCAGAGAGCCTGCGTAATGAAACCGTCCTGCAAATCACGGGTCTGGTCAGACCCAGGCCG
GCCGATGCAATCAACAGCAAACTCAGCACCGGCGAGATTGAGGTGCTAGCCAATGGTCTCGAGGTACTCAACCCCGTCAC
AGGCAACCTGCCCTTTACGGTCTCGATCCACGACGAAGAACCCGTCAAAGAAGAGCTACGTCTGCGTCACCGCCACCTGG
ATCTGCGCCGGGAACGAATGAGCCGCAATCTCCAGCTACGCCACACAACCATTAAAACGGCTCGTCATTTTTTGGAAGAC
GAAGGATTCATCGAAGTGGAAACTCCTGTTCTCACACGCTCCACTCCCGAAGGAGCCCGCGACTACCTAGTGCCTTCAAG
GGTATGCAGCGGTGAATGGTTTGCTCTGCCTCAATCCCCCCAGCTGTTCAAACAATTACTCATGGTGGGCGGATTGGAGC
GCTACTACCAAGTCGCTCGCTGCTTCCGCGATGAAGACCTACGCGCCGACAGGCAGCCGGAATTCACCCAGCTGGACATC
GAAATGAGTTTCATGGATCAAGAACAGATCCTCAAGCTCAATGAACGACTGATCGCCGCCATCTGGAAAGCAGTGAAGGG
CATCGACCTACCACTTCCCTTCCCAAGATTGACCTGGCATGAGGCCATGGACCGATACGGCACCGACCGACCCGATACCC
GCTATGGAATGGAGCTCAATGATGTCAGCGACATCCTCAAAGACATGGGCTTCAAAGTCTTCTCCGGGGCCATAGCCGCT
GGAGGCTCTGTGAAGTGCATCACTGTGCCAAACGGGAATGACCTGATCAGCAACGTGCGCATCAAACCCGGTGGAGACAT
TTTCAACGAAGCCCAAAAAGCTGGGGCCGGTGGCCTGGCTTTCATCCGTGTACGCGATTCCGATGAGATCGACAGCATCG
GTGCGATCAAAGACAACCTCAACAGCAAACAAAAGGCAGCCCTGCTGAAACAGACTGGAGCCAAAGCGGGTGACTTGATT
TTGTTCGGAGCAGGCAAGACCGCCACCGTCAACAGCGCCCTTGACCGGGTGCGGCAATTCCTGGGCCGCGAGTTGGGCCT
GATCCCCACAGACCAAGACAACAAGCTATGGCAGTTCCTCTGGGTCGTGGACTTCCCGATGTTCGAGTTGAACGCAGAAG
AAAACCGCCTTGAGGCTCTCCATCACCCCTTCTGTGCACCAAATAAAGACGATCTAGGCGACGATCCCGACGCCTGGATG
GAGCGCCTACCTCAAGCCCGTGCTCAGGCCTATGACCTTGTCTTGAATGGGCTGGAGCTTGGCGGCGGATCACTACGCAT
CCACAATGCCGAACTGCAACGACAAGTGCTGCAAACCATCGGTCTGCCCCTAGAAGAAGCAAAGCAACAATTCGGCTTCC
TCATCGATGCCCTTGAGATGGGAGCCCCACCCCATGGCGGCTTGGCATTCGGGATGGATCGGATCGTCATGCTGCTCACA
GGAGAAGACTCGATCCGCGACACGATTGCCTTCCCAAAAACCCAACAAGCTCGCTGCCTGATGACCCAAGCCCCCGCAGG
TGTCTCCAACCATCAATTAGAGGAACTGCATGTAGAAAGCACCTGGGTCGATCCCGAATAG

Upstream 100 bases:

>100_bases
CGTCCTTCCCTTAGCTGAAGGCCATCGCCAAAACCGGTCGTTCTAATAAGCGGCAACAAGCCCCTGTGGGAAACTCGCTT
TTCATCCTGAAGCCTTCCCC

Downstream 100 bases:

>100_bases
ATTCAGCGCATTCCAGACAACAAGGGGCGTCTTCATTAACCCTGAAGGCATATCTTCGCGGGCAACCCTTGAAGAGCAGC
CCCCAGCAAACCAAGACTTC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MRSNGCGELRSKHIASNVKLCGWVDRCRDHGGVIFIDLRDRSGTIQITVDPDQGTELFASAESLRNETVLQITGLVRPRP
ADAINSKLSTGEIEVLANGLEVLNPVTGNLPFTVSIHDEEPVKEELRLRHRHLDLRRERMSRNLQLRHTTIKTARHFLED
EGFIEVETPVLTRSTPEGARDYLVPSRVCSGEWFALPQSPQLFKQLLMVGGLERYYQVARCFRDEDLRADRQPEFTQLDI
EMSFMDQEQILKLNERLIAAIWKAVKGIDLPLPFPRLTWHEAMDRYGTDRPDTRYGMELNDVSDILKDMGFKVFSGAIAA
GGSVKCITVPNGNDLISNVRIKPGGDIFNEAQKAGAGGLAFIRVRDSDEIDSIGAIKDNLNSKQKAALLKQTGAKAGDLI
LFGAGKTATVNSALDRVRQFLGRELGLIPTDQDNKLWQFLWVVDFPMFELNAEENRLEALHHPFCAPNKDDLGDDPDAWM
ERLPQARAQAYDLVLNGLELGGGSLRIHNAELQRQVLQTIGLPLEEAKQQFGFLIDALEMGAPPHGGLAFGMDRIVMLLT
GEDSIRDTIAFPKTQQARCLMTQAPAGVSNHQLEELHVESTWVDPE

Sequences:

>Translated_606_residues
MRSNGCGELRSKHIASNVKLCGWVDRCRDHGGVIFIDLRDRSGTIQITVDPDQGTELFASAESLRNETVLQITGLVRPRP
ADAINSKLSTGEIEVLANGLEVLNPVTGNLPFTVSIHDEEPVKEELRLRHRHLDLRRERMSRNLQLRHTTIKTARHFLED
EGFIEVETPVLTRSTPEGARDYLVPSRVCSGEWFALPQSPQLFKQLLMVGGLERYYQVARCFRDEDLRADRQPEFTQLDI
EMSFMDQEQILKLNERLIAAIWKAVKGIDLPLPFPRLTWHEAMDRYGTDRPDTRYGMELNDVSDILKDMGFKVFSGAIAA
GGSVKCITVPNGNDLISNVRIKPGGDIFNEAQKAGAGGLAFIRVRDSDEIDSIGAIKDNLNSKQKAALLKQTGAKAGDLI
LFGAGKTATVNSALDRVRQFLGRELGLIPTDQDNKLWQFLWVVDFPMFELNAEENRLEALHHPFCAPNKDDLGDDPDAWM
ERLPQARAQAYDLVLNGLELGGGSLRIHNAELQRQVLQTIGLPLEEAKQQFGFLIDALEMGAPPHGGLAFGMDRIVMLLT
GEDSIRDTIAFPKTQQARCLMTQAPAGVSNHQLEELHVESTWVDPE
>Mature_606_residues
MRSNGCGELRSKHIASNVKLCGWVDRCRDHGGVIFIDLRDRSGTIQITVDPDQGTELFASAESLRNETVLQITGLVRPRP
ADAINSKLSTGEIEVLANGLEVLNPVTGNLPFTVSIHDEEPVKEELRLRHRHLDLRRERMSRNLQLRHTTIKTARHFLED
EGFIEVETPVLTRSTPEGARDYLVPSRVCSGEWFALPQSPQLFKQLLMVGGLERYYQVARCFRDEDLRADRQPEFTQLDI
EMSFMDQEQILKLNERLIAAIWKAVKGIDLPLPFPRLTWHEAMDRYGTDRPDTRYGMELNDVSDILKDMGFKVFSGAIAA
GGSVKCITVPNGNDLISNVRIKPGGDIFNEAQKAGAGGLAFIRVRDSDEIDSIGAIKDNLNSKQKAALLKQTGAKAGDLI
LFGAGKTATVNSALDRVRQFLGRELGLIPTDQDNKLWQFLWVVDFPMFELNAEENRLEALHHPFCAPNKDDLGDDPDAWM
ERLPQARAQAYDLVLNGLELGGGSLRIHNAELQRQVLQTIGLPLEEAKQQFGFLIDALEMGAPPHGGLAFGMDRIVMLLT
GEDSIRDTIAFPKTQQARCLMTQAPAGVSNHQLEELHVESTWVDPE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=619, Percent_Identity=41.0339256865913, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI5031815, Length=319, Percent_Identity=24.7648902821317, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI194272210, Length=319, Percent_Identity=24.7648902821317, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI45439306, Length=243, Percent_Identity=25.5144032921811, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI226491502, Length=253, Percent_Identity=24.5059288537549, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788173, Length=603, Percent_Identity=47.7611940298507, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1789256, Length=290, Percent_Identity=28.9655172413793, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790571, Length=299, Percent_Identity=29.0969899665552, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=608, Percent_Identity=35.3618421052632, Blast_Score=338, Evalue=4e-93,
Organism=Caenorhabditis elegans, GI71994340, Length=317, Percent_Identity=25.8675078864353, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17535925, Length=317, Percent_Identity=25.8675078864353, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17535927, Length=317, Percent_Identity=25.8675078864353, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17551876, Length=258, Percent_Identity=24.031007751938, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=664, Percent_Identity=30.7228915662651, Blast_Score=255, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6321807, Length=247, Percent_Identity=28.3400809716599, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=242, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324256, Length=255, Percent_Identity=24.7058823529412, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24584738, Length=608, Percent_Identity=39.9671052631579, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24640849, Length=270, Percent_Identity=29.2592592592593, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24640851, Length=270, Percent_Identity=29.2592592592593, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17136276, Length=263, Percent_Identity=25.8555133079848, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921528, Length=234, Percent_Identity=28.6324786324786, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67682; Mature: 67682

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSNGCGELRSKHIASNVKLCGWVDRCRDHGGVIFIDLRDRSGTIQITVDPDQGTELFAS
CCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCCHHHHH
AESLRNETVLQITGLVRPRPADAINSKLSTGEIEVLANGLEVLNPVTGNLPFTVSIHDEE
HHHHCCCEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCC
PVKEELRLRHRHLDLRRERMSRNLQLRHTTIKTARHFLEDEGFIEVETPVLTRSTPEGAR
CHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCH
DYLVPSRVCSGEWFALPQSPQLFKQLLMVGGLERYYQVARCFRDEDLRADRQPEFTQLDI
HCCCCCHHCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
EMSFMDQEQILKLNERLIAAIWKAVKGIDLPLPFPRLTWHEAMDRYGTDRPDTRYGMELN
EEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHH
DVSDILKDMGFKVFSGAIAAGGSVKCITVPNGNDLISNVRIKPGGDIFNEAQKAGAGGLA
HHHHHHHHCCHHHHHHHEECCCCEEEEECCCCCCHHHCCEECCCCHHHHHHHHCCCCCEE
FIRVRDSDEIDSIGAIKDNLNSKQKAALLKQTGAKAGDLILFGAGKTATVNSALDRVRQF
EEEEECCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHH
LGRELGLIPTDQDNKLWQFLWVVDFPMFELNAEENRLEALHHPFCAPNKDDLGDDPDAWM
HCHHCCCCCCCCCCCCEEEEEEECCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHH
ERLPQARAQAYDLVLNGLELGGGSLRIHNAELQRQVLQTIGLPLEEAKQQFGFLIDALEM
HHHHHHHHHHHHHHHHCEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GAPPHGGLAFGMDRIVMLLTGEDSIRDTIAFPKTQQARCLMTQAPAGVSNHQLEELHVES
CCCCCCCHHCCCCEEEEEEECCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHEEEEC
TWVDPE
CCCCCC
>Mature Secondary Structure
MRSNGCGELRSKHIASNVKLCGWVDRCRDHGGVIFIDLRDRSGTIQITVDPDQGTELFAS
CCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCCHHHHH
AESLRNETVLQITGLVRPRPADAINSKLSTGEIEVLANGLEVLNPVTGNLPFTVSIHDEE
HHHHCCCEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCC
PVKEELRLRHRHLDLRRERMSRNLQLRHTTIKTARHFLEDEGFIEVETPVLTRSTPEGAR
CHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCH
DYLVPSRVCSGEWFALPQSPQLFKQLLMVGGLERYYQVARCFRDEDLRADRQPEFTQLDI
HCCCCCHHCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
EMSFMDQEQILKLNERLIAAIWKAVKGIDLPLPFPRLTWHEAMDRYGTDRPDTRYGMELN
EEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHH
DVSDILKDMGFKVFSGAIAAGGSVKCITVPNGNDLISNVRIKPGGDIFNEAQKAGAGGLA
HHHHHHHHCCHHHHHHHEECCCCEEEEECCCCCCHHHCCEECCCCHHHHHHHHCCCCCEE
FIRVRDSDEIDSIGAIKDNLNSKQKAALLKQTGAKAGDLILFGAGKTATVNSALDRVRQF
EEEEECCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHH
LGRELGLIPTDQDNKLWQFLWVVDFPMFELNAEENRLEALHHPFCAPNKDDLGDDPDAWM
HCHHCCCCCCCCCCCCEEEEEEECCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHH
ERLPQARAQAYDLVLNGLELGGGSLRIHNAELQRQVLQTIGLPLEEAKQQFGFLIDALEM
HHHHHHHHHHHHHHHHCEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GAPPHGGLAFGMDRIVMLLTGEDSIRDTIAFPKTQQARCLMTQAPAGVSNHQLEELHVES
CCCCCCCHHCCCCEEEEEEECCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHEEEEC
TWVDPE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA