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The map label for this gene is sigC [H]

Identifier: 33864481

GI number: 33864481

Start: 2344770

End: 2345900

Strand: Direct

Name: sigC [H]

Synonym: PMT2217

Alternate gene names: 33864481

Gene position: 2344770-2345900 (Clockwise)

Preceding gene: 33864480

Following gene: 33864482

Centisome position: 97.26

GC content: 53.85

Gene sequence:

>1131_bases
TTGAAGAGCAGCCCCCAGCAAACCAAGACTTCCCGAGTCCGCAGAAGCGGCAGCACAGATCCTGTGCGTCTCTACTTGCA
GGACATTGGACGCGTAGAGCTACTAACCAATGAAGAGGAAGTGACCCTGGCGAGACTTGTGCAGCGCAGGGAAGCCCTAC
TCAAACAGGAAAGGAAGCTGGCCTCCAACCAAGAAGCGATCAAAGAATTACAAAGACTGGAGGAGCTGCAGCAACGAGAA
GCGAACCATTCATGCCACTGGCCCACCAAACAGGAATGGGCGATGGCTGCTGGGCTCACCCTGGCCGAGCTGCAAGAGAA
AATCGAGACTGGTTACAAAACCTGGGGAGCCCTGACTGGTCTTGATCCTTTGGAACTCAAGCGAAGTTTGCGAGCTGGTC
GGCGTGCCAAGGATCAGATGATCCAGGCCAATCTTCGACTTGTGGTGGCTGTAGCCAAGAAATATCAACAACGGGGCATA
GAGCTGCTGGATCTGGTGCAAGAAGGCACCCTGGGCTTGGAACGCGCAGTAGAGAAGTTCGACCCCACCAGAGGTTTCCG
CTTCAGCACCTACGCCTACTGGTGGATCCGTCAGGGCATCACCAGGGCCATTGCGACGCAAAGTCGGACGATCCGACTGC
CGATGCACATCACCGAAAAACTAAACCGCATCAAACGGGTTCAACAGGAGATTGCTAGCAGCCAAGGACGATTAGCTTCG
ATTGCCGATCTCGCCAAGGCACTTGGCCTTAGTGAAGAAACAGTGCGCCTAACCCTAATGAGGGTTCCACGTTCGATCTC
CCTGGAAACTCGAATAGGCCAAGAACAAGACACCCAACTCGGCGATCTGCTGGAAGACAGCAACGCGACCCCAGAGGAGA
AACTCACCCGCAATCAATTGCACAACGACCTTGAAATCTTGCTGGATGAACTAAGCAACCGCGAAGCGACAGTCATCAGA
CGACGTTTTGGACTTGAAGACGACACTCCGCAAACCCTGACGCAAATCGGCGAGGCAATGCATCTATCGCGAGAACGAGT
GCGTCAGATTGAAACCCATGCCCTCTTGAAATTGCGTCAACCGCAACGTCGCTGCAAGGTACGGGACTACATTCAAAATC
TCGATTCCTGA

Upstream 100 bases:

>100_bases
ATGTAGAAAGCACCTGGGTCGATCCCGAATAGATTCAGCGCATTCCAGACAACAAGGGGCGTCTTCATTAACCCTGAAGG
CATATCTTCGCGGGCAACCC

Downstream 100 bases:

>100_bases
TTAACACTTCACAAACGCACTGAATTTCAATACCTCAGACCTCCTTCATTGAAATCGGCATCCCAGCAGACAAACGAAAG
GCAATCTCTGATGGCCTTGG

Product: type II alternative RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKSSPQQTKTSRVRRSGSTDPVRLYLQDIGRVELLTNEEEVTLARLVQRREALLKQERKLASNQEAIKELQRLEELQQRE
ANHSCHWPTKQEWAMAAGLTLAELQEKIETGYKTWGALTGLDPLELKRSLRAGRRAKDQMIQANLRLVVAVAKKYQQRGI
ELLDLVQEGTLGLERAVEKFDPTRGFRFSTYAYWWIRQGITRAIATQSRTIRLPMHITEKLNRIKRVQQEIASSQGRLAS
IADLAKALGLSEETVRLTLMRVPRSISLETRIGQEQDTQLGDLLEDSNATPEEKLTRNQLHNDLEILLDELSNREATVIR
RRFGLEDDTPQTLTQIGEAMHLSRERVRQIETHALLKLRQPQRRCKVRDYIQNLDS

Sequences:

>Translated_376_residues
MKSSPQQTKTSRVRRSGSTDPVRLYLQDIGRVELLTNEEEVTLARLVQRREALLKQERKLASNQEAIKELQRLEELQQRE
ANHSCHWPTKQEWAMAAGLTLAELQEKIETGYKTWGALTGLDPLELKRSLRAGRRAKDQMIQANLRLVVAVAKKYQQRGI
ELLDLVQEGTLGLERAVEKFDPTRGFRFSTYAYWWIRQGITRAIATQSRTIRLPMHITEKLNRIKRVQQEIASSQGRLAS
IADLAKALGLSEETVRLTLMRVPRSISLETRIGQEQDTQLGDLLEDSNATPEEKLTRNQLHNDLEILLDELSNREATVIR
RRFGLEDDTPQTLTQIGEAMHLSRERVRQIETHALLKLRQPQRRCKVRDYIQNLDS
>Mature_376_residues
MKSSPQQTKTSRVRRSGSTDPVRLYLQDIGRVELLTNEEEVTLARLVQRREALLKQERKLASNQEAIKELQRLEELQQRE
ANHSCHWPTKQEWAMAAGLTLAELQEKIETGYKTWGALTGLDPLELKRSLRAGRRAKDQMIQANLRLVVAVAKKYQQRGI
ELLDLVQEGTLGLERAVEKFDPTRGFRFSTYAYWWIRQGITRAIATQSRTIRLPMHITEKLNRIKRVQQEIASSQGRLAS
IADLAKALGLSEETVRLTLMRVPRSISLETRIGQEQDTQLGDLLEDSNATPEEKLTRNQLHNDLEILLDELSNREATVIR
RRFGLEDDTPQTLTQIGEAMHLSRERVRQIETHALLKLRQPQRRCKVRDYIQNLDS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=242, Percent_Identity=44.2148760330579, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI1789098, Length=235, Percent_Identity=42.9787234042553, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=26.0330578512397, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 43382; Mature: 43382

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSPQQTKTSRVRRSGSTDPVRLYLQDIGRVELLTNEEEVTLARLVQRREALLKQERKL
CCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
ASNQEAIKELQRLEELQQREANHSCHWPTKQEWAMAAGLTLAELQEKIETGYKTWGALTG
HCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
LDPLELKRSLRAGRRAKDQMIQANLRLVVAVAKKYQQRGIELLDLVQEGTLGLERAVEKF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHC
DPTRGFRFSTYAYWWIRQGITRAIATQSRTIRLPMHITEKLNRIKRVQQEIASSQGRLAS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCHHH
IADLAKALGLSEETVRLTLMRVPRSISLETRIGQEQDTQLGDLLEDSNATPEEKLTRNQL
HHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
HNDLEILLDELSNREATVIRRRFGLEDDTPQTLTQIGEAMHLSRERVRQIETHALLKLRQ
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PQRRCKVRDYIQNLDS
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSSPQQTKTSRVRRSGSTDPVRLYLQDIGRVELLTNEEEVTLARLVQRREALLKQERKL
CCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
ASNQEAIKELQRLEELQQREANHSCHWPTKQEWAMAAGLTLAELQEKIETGYKTWGALTG
HCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
LDPLELKRSLRAGRRAKDQMIQANLRLVVAVAKKYQQRGIELLDLVQEGTLGLERAVEKF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHC
DPTRGFRFSTYAYWWIRQGITRAIATQSRTIRLPMHITEKLNRIKRVQQEIASSQGRLAS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCHHH
IADLAKALGLSEETVRLTLMRVPRSISLETRIGQEQDTQLGDLLEDSNATPEEKLTRNQL
HHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
HNDLEILLDELSNREATVIRRRFGLEDDTPQTLTQIGEAMHLSRERVRQIETHALLKLRQ
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PQRRCKVRDYIQNLDS
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1385387; 11759840 [H]