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The map label for this gene is thiO [H]

Identifier: 33864404

GI number: 33864404

Start: 2252306

End: 2253367

Strand: Direct

Name: thiO [H]

Synonym: PMT2140

Alternate gene names: 33864404

Gene position: 2252306-2253367 (Clockwise)

Preceding gene: 33864402

Following gene: 33864405

Centisome position: 93.42

GC content: 56.12

Gene sequence:

>1062_bases
ATGGGCCTAGCTTTAGCCCACAAATTGGCACGGCGCGGCAGCGCAGTAGAAGTCCTCAGCAGACGCCGCAATGAGGCCGC
TGGCTTCGTTGCGGCCGGCATGCTTGCACCCCATGCTGAAGGCCTCAGCGGAGAGCAACTACGGCTAGGCCAATTAAGCC
TGGATCTCATCCCAGCCTGGGTGAACCAAATCGAAACCGACAGCGGCCTCTGTTGCGGTCTGCGCCAATGCGGGATTGTG
GTGCCCTTCGCCACAGCTACGCAGCGAGATCTCTATCCCACCGCCTACTTAGGTGAGACCCTCGATCGTTCAGGCCTGGA
GCGAGAAGTACCAGGCATTGGACCTAACTGGCAGGCCGGCTTGCTCTTCAGCCAAGATGGCCAAATTGACAACCGAAGGC
AATTAATGCGGGCCCTGGAGACAGCCTGCGTGAGCCTTGGAGTTCGCTTCCAGGAAGGGGTGAACGTGCAAGAACTCCTT
CAAGATGGCAATACCTTTAAGGGAGTGCGCGTACGCAACGCTGAAGGAGAGCTACACACACTTACTAGTAAAGAAGCTGT
GCTCTGCTGCGGTGCCTGGAGCGCCCAACTGCTCCCAGAGCTATCTATTTACCCGGTAAAAGGCCAAATGCTTTCGCTCC
AAGGGCCACGAAATGCCTTAACACGAATTGTGTTTGGCCCAGGCACTTACCTCGTACCTCGTGAAGATGGGCTACTCGTG
GTGGGAGCCACCAGCGAACCAGATGCCGGCTTTACAGAAGGCCTCACCCCTTTTGGCCAACGACAACTGCAAGCAGGCAT
TGCTTCACTGCTACCGCAGGCAAACCAATGGCCACCGATGGAGCGCTGGTGGGGGTTCCGACCTTGCACACCTGACGGAG
GACCACTACTTGGCCGCTCATCCGTTGATGGCCTCTGGCTGGCAACTGGACATCACCGTAATGGAGTGCTGCTCGCCGCC
ATCACAGCTGAATTAATCGAAACATGCATCAGCAGCAAGGGAATCAACGCAGAACAAATTCAGATACTAGAAGCATTTCA
GTGGAATCGATTTACGGCATAA

Upstream 100 bases:

>100_bases
GGGTCAACAGGCTGAAACACTGAACTGATCTGGACTGTTCTTTGCATTGACGATGGCAGCAATGCGTACTGAATCAGTAC
TGATCCTGGGAGGCGGCTTG

Downstream 100 bases:

>100_bases
ATATGCCAATTAAACCATGCAGCGATGGATCCATGACAATAATTTCTCTTCGTCAATTGACTTCAGAAATTTGAGTTGAA
TCGCAATTCAATCCACACCA

Product: thiamine biosynthesis oxidoreductase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MGLALAHKLARRGSAVEVLSRRRNEAAGFVAAGMLAPHAEGLSGEQLRLGQLSLDLIPAWVNQIETDSGLCCGLRQCGIV
VPFATATQRDLYPTAYLGETLDRSGLEREVPGIGPNWQAGLLFSQDGQIDNRRQLMRALETACVSLGVRFQEGVNVQELL
QDGNTFKGVRVRNAEGELHTLTSKEAVLCCGAWSAQLLPELSIYPVKGQMLSLQGPRNALTRIVFGPGTYLVPREDGLLV
VGATSEPDAGFTEGLTPFGQRQLQAGIASLLPQANQWPPMERWWGFRPCTPDGGPLLGRSSVDGLWLATGHHRNGVLLAA
ITAELIETCISSKGINAEQIQILEAFQWNRFTA

Sequences:

>Translated_353_residues
MGLALAHKLARRGSAVEVLSRRRNEAAGFVAAGMLAPHAEGLSGEQLRLGQLSLDLIPAWVNQIETDSGLCCGLRQCGIV
VPFATATQRDLYPTAYLGETLDRSGLEREVPGIGPNWQAGLLFSQDGQIDNRRQLMRALETACVSLGVRFQEGVNVQELL
QDGNTFKGVRVRNAEGELHTLTSKEAVLCCGAWSAQLLPELSIYPVKGQMLSLQGPRNALTRIVFGPGTYLVPREDGLLV
VGATSEPDAGFTEGLTPFGQRQLQAGIASLLPQANQWPPMERWWGFRPCTPDGGPLLGRSSVDGLWLATGHHRNGVLLAA
ITAELIETCISSKGINAEQIQILEAFQWNRFTA
>Mature_352_residues
GLALAHKLARRGSAVEVLSRRRNEAAGFVAAGMLAPHAEGLSGEQLRLGQLSLDLIPAWVNQIETDSGLCCGLRQCGIVV
PFATATQRDLYPTAYLGETLDRSGLEREVPGIGPNWQAGLLFSQDGQIDNRRQLMRALETACVSLGVRFQEGVNVQELLQ
DGNTFKGVRVRNAEGELHTLTSKEAVLCCGAWSAQLLPELSIYPVKGQMLSLQGPRNALTRIVFGPGTYLVPREDGLLVV
GATSEPDAGFTEGLTPFGQRQLQAGIASLLPQANQWPPMERWWGFRPCTPDGGPLLGRSSVDGLWLATGHHRNGVLLAAI
TAELIETCISSKGINAEQIQILEAFQWNRFTA

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=193, Percent_Identity=27.979274611399, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 38174; Mature: 38043

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLALAHKLARRGSAVEVLSRRRNEAAGFVAAGMLAPHAEGLSGEQLRLGQLSLDLIPAW
CCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHH
VNQIETDSGLCCGLRQCGIVVPFATATQRDLYPTAYLGETLDRSGLEREVPGIGPNWQAG
HHHHCCCCCCEECHHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEE
LLFSQDGQIDNRRQLMRALETACVSLGVRFQEGVNVQELLQDGNTFKGVRVRNAEGELHT
EEECCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHCCCCCCCEEEECCCCCEEE
LTSKEAVLCCGAWSAQLLPELSIYPVKGQMLSLQGPRNALTRIVFGPGTYLVPREDGLLV
ECCCCEEEEECCCHHHHCCCCEEEECCCEEEEECCCHHHEEEEEECCCCEEEECCCCEEE
VGATSEPDAGFTEGLTPFGQRQLQAGIASLLPQANQWPPMERWWGFRPCTPDGGPLLGRS
EECCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCC
SVDGLWLATGHHRNGVLLAAITAELIETCISSKGINAEQIQILEAFQWNRFTA
CCCCEEEEECCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GLALAHKLARRGSAVEVLSRRRNEAAGFVAAGMLAPHAEGLSGEQLRLGQLSLDLIPAW
CHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHH
VNQIETDSGLCCGLRQCGIVVPFATATQRDLYPTAYLGETLDRSGLEREVPGIGPNWQAG
HHHHCCCCCCEECHHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEE
LLFSQDGQIDNRRQLMRALETACVSLGVRFQEGVNVQELLQDGNTFKGVRVRNAEGELHT
EEECCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHCCCCCCCEEEECCCCCEEE
LTSKEAVLCCGAWSAQLLPELSIYPVKGQMLSLQGPRNALTRIVFGPGTYLVPREDGLLV
ECCCCEEEEECCCHHHHCCCCEEEECCCEEEEECCCHHHEEEEEECCCCEEEECCCCEEE
VGATSEPDAGFTEGLTPFGQRQLQAGIASLLPQANQWPPMERWWGFRPCTPDGGPLLGRS
EECCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCC
SVDGLWLATGHHRNGVLLAAITAELIETCISSKGINAEQIQILEAFQWNRFTA
CCCCEEEEECCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA