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The map label for this gene is trmE

Identifier: 33864338

GI number: 33864338

Start: 2181646

End: 2183058

Strand: Reverse

Name: trmE

Synonym: PMT2074

Alternate gene names: 33864338

Gene position: 2183058-2181646 (Counterclockwise)

Preceding gene: 33864340

Following gene: 33864337

Centisome position: 90.55

GC content: 57.25

Gene sequence:

>1413_bases
ATGATGTCTTCTCCAATCCCCCATCAACAAACGATTGCCGCAGTGGCAACGGCGGTTGCACCAGGCCAAGGGGGTATTGC
TGTGGTGCGGCTTTCCGGGCCAGCGGCTGAGGTCGTTGGGCGGAGTGTGGTGTCGATCCCGGGGCAACAACTTTGGGTTA
GTCATCGTGTGTTGTATGGCCATGTGATGGATGAGAGCGGCAAGGAACGGATCGATGAAGTTCTTGTGTTGCTGATGAAG
GGGCCACGCAGTTTCACTGGCGAGGATGTTGTGGAGATCCACTGTCATGGTGGATTGATGGCTGTTCAGCGGGTGCTTGA
GCGTGTGTTGGCTCAGCCGCATGTGCGCCGGGCATTGCCTGGAGAGTTCAGCCAGCGGGCGGTGCTCAACGGCCGGCTTG
ACCTCACCCAGGCGGAGGCGATCAGTGAACTTGTGGCGGCGCGTAGTCGTCGAGCAGCCCAGTTGGCGATGACGGGAGTC
GATGGAGGCATTCAGCGCCGCATCACCTCCCTGCGGGAAAGGTTGTTGGATCAGCTCAGTGAGCTGGAGGCCAGGGTTGA
TTTTGAGGAGGATCTTCCCCCTCTCGATGGTGCTGAGTTGTTACTTGAGTTGCAGTGCGTGCGCAGAGAGCTCGAACAGC
TTGTTGAAGATGCCAAGCGTGGTGATGTGCTGCGCCAGGGGCTACAGGTGGCTCTGGTTGGCCGCCCCAATGTTGGTAAG
AGCTCCCTGTTGAATCGCCTCAGCCGCCGGGAGCGGGCGATCGTGACGGATCTTCCTGGTACGACCAGGGATGTCTTGGA
GAGCGAGATTGTGCTGGAGGGTGTGCCGATCACCTTGGTAGACACCGCAGGCATTCGCGCCACGCAGGATGCTCTGGAGC
AACTAGGGATAGATCGTAGTCATCAGGCTCTCGCTGCAGCTGATGTGGCGGTGTTGGTGTTTGATCTCAGTCTGGGATGG
ACAGCCGACGATGCGGCGTTGCTCGCTCAGATCCCAGATGATCTGCCAAGGCTTCTTGTGGGTAATAAAGCTGATCTTCA
GCCTGCCTCGATGGCTGCTTCTCTAATGGTTGCTTCGCTGGGCAATGAGGTTGATGGCAAGACTGTGGATGTGATGTTGA
GTGCACTCACTGGGCAAGGAGAAGAGGCCTTGATTAAGGCTGTTTTGAAGACTTGTGGTGCCAGCGAAGCGCAGGGGTTG
GTGGTTGCGCTCAATCAACGGCAGCAAGATCTTGCCGCAGCAGCAGCAATTGCTTTGGCTCGCACGCAGGAAGCAGCAGA
GCACCAGTTGCCTTGGGATTTCTGGACCATTGATCTGCGGCAAGCGATCAGCAGCTTGGGTGAGATCACTGGCGAGGAGA
TCACAGAGGCTGTGCTTGATCGCATCTTTTCGCGGTTTTGTATTGGCAAATAA

Upstream 100 bases:

>100_bases
CGCCTGCTGATGCCCTGGAGGGTTTGCTGGATCATGGCCAGACCGAAGCAATGGTGCTGCAGGGTGGCTTTGATCGTAGG
AAGGGCTGAAGGTGCCAGTG

Downstream 100 bases:

>100_bases
GTGAAAATAGAAACGCACTAGTGTGAATCAATAGTGGCAAAACGCTTTTTTCTGCCTAGCCAATCACGCTTGGAAAGATT
GTATTTTTGTTTGCAAGATG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MMSSPIPHQQTIAAVATAVAPGQGGIAVVRLSGPAAEVVGRSVVSIPGQQLWVSHRVLYGHVMDESGKERIDEVLVLLMK
GPRSFTGEDVVEIHCHGGLMAVQRVLERVLAQPHVRRALPGEFSQRAVLNGRLDLTQAEAISELVAARSRRAAQLAMTGV
DGGIQRRITSLRERLLDQLSELEARVDFEEDLPPLDGAELLLELQCVRRELEQLVEDAKRGDVLRQGLQVALVGRPNVGK
SSLLNRLSRRERAIVTDLPGTTRDVLESEIVLEGVPITLVDTAGIRATQDALEQLGIDRSHQALAAADVAVLVFDLSLGW
TADDAALLAQIPDDLPRLLVGNKADLQPASMAASLMVASLGNEVDGKTVDVMLSALTGQGEEALIKAVLKTCGASEAQGL
VVALNQRQQDLAAAAAIALARTQEAAEHQLPWDFWTIDLRQAISSLGEITGEEITEAVLDRIFSRFCIGK

Sequences:

>Translated_470_residues
MMSSPIPHQQTIAAVATAVAPGQGGIAVVRLSGPAAEVVGRSVVSIPGQQLWVSHRVLYGHVMDESGKERIDEVLVLLMK
GPRSFTGEDVVEIHCHGGLMAVQRVLERVLAQPHVRRALPGEFSQRAVLNGRLDLTQAEAISELVAARSRRAAQLAMTGV
DGGIQRRITSLRERLLDQLSELEARVDFEEDLPPLDGAELLLELQCVRRELEQLVEDAKRGDVLRQGLQVALVGRPNVGK
SSLLNRLSRRERAIVTDLPGTTRDVLESEIVLEGVPITLVDTAGIRATQDALEQLGIDRSHQALAAADVAVLVFDLSLGW
TADDAALLAQIPDDLPRLLVGNKADLQPASMAASLMVASLGNEVDGKTVDVMLSALTGQGEEALIKAVLKTCGASEAQGL
VVALNQRQQDLAAAAAIALARTQEAAEHQLPWDFWTIDLRQAISSLGEITGEEITEAVLDRIFSRFCIGK
>Mature_470_residues
MMSSPIPHQQTIAAVATAVAPGQGGIAVVRLSGPAAEVVGRSVVSIPGQQLWVSHRVLYGHVMDESGKERIDEVLVLLMK
GPRSFTGEDVVEIHCHGGLMAVQRVLERVLAQPHVRRALPGEFSQRAVLNGRLDLTQAEAISELVAARSRRAAQLAMTGV
DGGIQRRITSLRERLLDQLSELEARVDFEEDLPPLDGAELLLELQCVRRELEQLVEDAKRGDVLRQGLQVALVGRPNVGK
SSLLNRLSRRERAIVTDLPGTTRDVLESEIVLEGVPITLVDTAGIRATQDALEQLGIDRSHQALAAADVAVLVFDLSLGW
TADDAALLAQIPDDLPRLLVGNKADLQPASMAASLMVASLGNEVDGKTVDVMLSALTGQGEEALIKAVLKTCGASEAQGL
VVALNQRQQDLAAAAAIALARTQEAAEHQLPWDFWTIDLRQAISSLGEITGEEITEAVLDRIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=476, Percent_Identity=34.6638655462185, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI193082993, Length=468, Percent_Identity=34.4017094017094, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI193082988, Length=508, Percent_Identity=32.6771653543307, Blast_Score=198, Evalue=8e-51,
Organism=Escherichia coli, GI2367268, Length=464, Percent_Identity=37.7155172413793, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI87082120, Length=134, Percent_Identity=39.5522388059701, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=465, Percent_Identity=30.3225806451613, Blast_Score=181, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=529, Percent_Identity=29.4896030245747, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI45550813, Length=473, Percent_Identity=32.1353065539112, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_PROMM (Q7V491)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895898.1
- ProteinModelPortal:   Q7V491
- SMR:   Q7V491
- STRING:   Q7V491
- GeneID:   1727683
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT2074
- NMPDR:   fig|74547.1.peg.2065
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   PMAR74547:PMT2074-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 50500; Mature: 50500

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 92-92 BINDING 132-132 BINDING 470-470

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSSPIPHQQTIAAVATAVAPGQGGIAVVRLSGPAAEVVGRSVVSIPGQQLWVSHRVLYG
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHCCHHHHCCCHHHHHHHHHHHH
HVMDESGKERIDEVLVLLMKGPRSFTGEDVVEIHCHGGLMAVQRVLERVLAQPHVRRALP
HHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHCCC
GEFSQRAVLNGRLDLTQAEAISELVAARSRRAAQLAMTGVDGGIQRRITSLRERLLDQLS
CCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHH
ELEARVDFEEDLPPLDGAELLLELQCVRRELEQLVEDAKRGDVLRQGLQVALVGRPNVGK
HHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
SSLLNRLSRRERAIVTDLPGTTRDVLESEIVLEGVPITLVDTAGIRATQDALEQLGIDRS
HHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCC
HQALAAADVAVLVFDLSLGWTADDAALLAQIPDDLPRLLVGNKADLQPASMAASLMVASL
HHHHHHHHHHHHEEECCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
GNEVDGKTVDVMLSALTGQGEEALIKAVLKTCGASEAQGLVVALNQRQQDLAAAAAIALA
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHH
RTQEAAEHQLPWDFWTIDLRQAISSLGEITGEEITEAVLDRIFSRFCIGK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMSSPIPHQQTIAAVATAVAPGQGGIAVVRLSGPAAEVVGRSVVSIPGQQLWVSHRVLYG
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHCCHHHHCCCHHHHHHHHHHHH
HVMDESGKERIDEVLVLLMKGPRSFTGEDVVEIHCHGGLMAVQRVLERVLAQPHVRRALP
HHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHCCC
GEFSQRAVLNGRLDLTQAEAISELVAARSRRAAQLAMTGVDGGIQRRITSLRERLLDQLS
CCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHH
ELEARVDFEEDLPPLDGAELLLELQCVRRELEQLVEDAKRGDVLRQGLQVALVGRPNVGK
HHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
SSLLNRLSRRERAIVTDLPGTTRDVLESEIVLEGVPITLVDTAGIRATQDALEQLGIDRS
HHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCC
HQALAAADVAVLVFDLSLGWTADDAALLAQIPDDLPRLLVGNKADLQPASMAASLMVASL
HHHHHHHHHHHHEEECCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
GNEVDGKTVDVMLSALTGQGEEALIKAVLKTCGASEAQGLVVALNQRQQDLAAAAAIALA
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHH
RTQEAAEHQLPWDFWTIDLRQAISSLGEITGEEITEAVLDRIFSRFCIGK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642