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The map label for this gene is gshB [H]

Identifier: 33864326

GI number: 33864326

Start: 2170600

End: 2171556

Strand: Reverse

Name: gshB [H]

Synonym: PMT2061

Alternate gene names: 33864326

Gene position: 2171556-2170600 (Counterclockwise)

Preceding gene: 33864327

Following gene: 33864324

Centisome position: 90.07

GC content: 57.68

Gene sequence:

>957_bases
ATGGTTTGCTCCAAGGCAAGGTCTGAAGCCATGCGACAGCTGTTCGTCTTGGATCCCCTGCAGTGCATTCAACCTGCCAA
GGATTCCTCAGCTGCGTTGATGCAGGCGGCCCAACGGGCATCAATTGAGGTTTGGGCTTGTACTCCCGCTGATCTTCAGG
CTCGTGGAGATCAGCTCTCTGCGATTGCCGTCCCTGTGGTAGCTGAGCCTTGGATTAGCACCGGTGAGCCCCGCAGCCTT
CCTCTAACGGACTTCGCCTGCATCTGGATGCGTAAGGATCCCCCGGTCGATGAGGGCTATCTCTATGCCACCCATTTGCT
CGAGCTGGCAGAGCGGGCTGGTGTGTGCGTGCTCAATAGGCCAGCGGCGCTGCGGGCCTGGAATGAAAAGCTCGGTGCGC
TGCGCTTCAACAACCTGATGGCTCCCACGCTTGTGGCCAGTCGGGTCAGTGAGCTTGCTGCCTTTGCCCGTGAGCAAGAA
GAGGTGGTGCTCAAACCTTTAGGGGGTCGAGCTGGTCAGGGGCTGGTGCGAGTCGCTGGGGCCGCTCCCGGACTTGAGGC
CTTGCTGGAGTTGGTCACGGATCAGGAGCAGCTACCGGTGATGGTTCAGCGGTTTTTGCCGGCTGTCATTGAAGGAGACA
AGCGCATCCTGTTGGTGGATGGAGAGCCTCTTGGTGCCGTGAACCGTCGTCCTAAGGCTGGAGATTTTCGTAGCAACCTT
GCCATGGGTGGTCGCCCGGAACCCACAGAGCTCGACAGTCGTGAATTGCAGATCTGTGCAGAATTGGCACCCGTTTTGAG
AGAGCAGGGTTTGTTTTTTGTGGGAATTGATGTGATTGATGGCCTGCTGAGTGAGATCAATGTCACCAGTCCTACTGGCA
TTAGAGAGGTTGAGCGCCTCAAGGGGGTTCCCCTGGCTGATCAAGTGATTGCGCGTTTGCTGGTCAGTCTTGGGTGA

Upstream 100 bases:

>100_bases
CGGGCTGGGGGAAAAAACACGTTGCCCCAGATCTTTATCGATGATCTGAGCATTGGTGGCTGCGATGAGTTGCATGCCCT
TGAGGGGGCTCAAAAACTTG

Downstream 100 bases:

>100_bases
TCCTTGGCATCCAGATCGAGTTGGTCGGCCAGTACAGACAGCTTCATGCCAACTTCTTGTAATTCGCGTTCGACCACAGA
CCCTCGGACCAAACCAGCTC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MVCSKARSEAMRQLFVLDPLQCIQPAKDSSAALMQAAQRASIEVWACTPADLQARGDQLSAIAVPVVAEPWISTGEPRSL
PLTDFACIWMRKDPPVDEGYLYATHLLELAERAGVCVLNRPAALRAWNEKLGALRFNNLMAPTLVASRVSELAAFAREQE
EVVLKPLGGRAGQGLVRVAGAAPGLEALLELVTDQEQLPVMVQRFLPAVIEGDKRILLVDGEPLGAVNRRPKAGDFRSNL
AMGGRPEPTELDSRELQICAELAPVLREQGLFFVGIDVIDGLLSEINVTSPTGIREVERLKGVPLADQVIARLLVSLG

Sequences:

>Translated_318_residues
MVCSKARSEAMRQLFVLDPLQCIQPAKDSSAALMQAAQRASIEVWACTPADLQARGDQLSAIAVPVVAEPWISTGEPRSL
PLTDFACIWMRKDPPVDEGYLYATHLLELAERAGVCVLNRPAALRAWNEKLGALRFNNLMAPTLVASRVSELAAFAREQE
EVVLKPLGGRAGQGLVRVAGAAPGLEALLELVTDQEQLPVMVQRFLPAVIEGDKRILLVDGEPLGAVNRRPKAGDFRSNL
AMGGRPEPTELDSRELQICAELAPVLREQGLFFVGIDVIDGLLSEINVTSPTGIREVERLKGVPLADQVIARLLVSLG
>Mature_318_residues
MVCSKARSEAMRQLFVLDPLQCIQPAKDSSAALMQAAQRASIEVWACTPADLQARGDQLSAIAVPVVAEPWISTGEPRSL
PLTDFACIWMRKDPPVDEGYLYATHLLELAERAGVCVLNRPAALRAWNEKLGALRFNNLMAPTLVASRVSELAAFAREQE
EVVLKPLGGRAGQGLVRVAGAAPGLEALLELVTDQEQLPVMVQRFLPAVIEGDKRILLVDGEPLGAVNRRPKAGDFRSNL
AMGGRPEPTELDSRELQICAELAPVLREQGLFFVGIDVIDGLLSEINVTSPTGIREVERLKGVPLADQVIARLLVSLG

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=289, Percent_Identity=41.8685121107266, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34379; Mature: 34379

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVCSKARSEAMRQLFVLDPLQCIQPAKDSSAALMQAAQRASIEVWACTPADLQARGDQLS
CCCCHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHCCEEEEEECCHHHHHCCCCEE
AIAVPVVAEPWISTGEPRSLPLTDFACIWMRKDPPVDEGYLYATHLLELAERAGVCVLNR
EEEECEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEECC
PAALRAWNEKLGALRFNNLMAPTLVASRVSELAAFAREQEEVVLKPLGGRAGQGLVRVAG
CHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCCCEEECC
AAPGLEALLELVTDQEQLPVMVQRFLPAVIEGDKRILLVDGEPLGAVNRRPKAGDFRSNL
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHCC
AMGGRPEPTELDSRELQICAELAPVLREQGLFFVGIDVIDGLLSEINVTSPTGIREVERL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHH
KGVPLADQVIARLLVSLG
CCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MVCSKARSEAMRQLFVLDPLQCIQPAKDSSAALMQAAQRASIEVWACTPADLQARGDQLS
CCCCHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHCCEEEEEECCHHHHHCCCCEE
AIAVPVVAEPWISTGEPRSLPLTDFACIWMRKDPPVDEGYLYATHLLELAERAGVCVLNR
EEEECEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEECC
PAALRAWNEKLGALRFNNLMAPTLVASRVSELAAFAREQEEVVLKPLGGRAGQGLVRVAG
CHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCCCEEECC
AAPGLEALLELVTDQEQLPVMVQRFLPAVIEGDKRILLVDGEPLGAVNRRPKAGDFRSNL
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHCC
AMGGRPEPTELDSRELQICAELAPVLREQGLFFVGIDVIDGLLSEINVTSPTGIREVERL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHH
KGVPLADQVIARLLVSLG
CCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]