Click here to switch to the map view.

The map label for this gene is kpsF [C]

Identifier: 33864177

GI number: 33864177

Start: 2017279

End: 2018322

Strand: Direct

Name: kpsF [C]

Synonym: PMT1911

Alternate gene names: 33864177

Gene position: 2017279-2018322 (Clockwise)

Preceding gene: 33864175

Following gene: 33864178

Centisome position: 83.67

GC content: 56.7

Gene sequence:

>1044_bases
ATGGCGGTGGTATGCAGGAGAATTACCGCATCATCACGAACTTACAGTTTGTCTGCCCTCACCCGCTGCCTGCAGGAAGA
GGCTGCCGCCATCGCCGTCGCTGCCGAACGACTGAGCAGCAGCCAAGTAGAAAAGGCATTGGTCTTGCTTGAACGCTGTA
GTGATCAACGCGCCAAATTGGTGATCACCGGCGTAGGCAAAAGCGGCATTGTTGCACGCAAAATCGCTGCCACCTTTTCC
TCTATCGGCCTAATGGCCCTTTACCTAAACCCCCTCGATGCAATGCATGGCGATCTGGGAGTGGTCGCCCAAGAGGACGT
CTGCCTACTGCTCTCCAACAGCGGTGAGACTGCTGAACTGTTAGAAGTGCTTCCCCACCTCAAACGACGTGGCACCGCTC
GCATCGCTCTGGTTGGGAAGCCCGACTCATCGCTTGCACGCGGTAGTGACGTGGTGCTTGAAGCAAGTGTTGATCGAGAA
GTGTGTCCGTTGAATCTTGCCCCCACCGCCAGCACAGCGGTGGCCATGGCGATCGGTGATGCCCTTGCTGCGATATGGAT
GGAACGTCGCAACATCTCACCTGCAGACTTCGCGTTCAACCACCCAGCCGGTTCGCTCGGCAAACAGCTCACCCTCACCG
CTTCGGACCTAATGGTGCCAGTTGAAAAGGTCCAGCCACTACAACCCAACACCAGTCTGCAAGAAGTGATCTGCAAGCTG
ACCCAAGATGGTATTGGTAGTGGCTGGGTGGAAGACCCCTCCACCGCCGGACTGCTGCTGGGCCTCATTACCGATGGTGA
TCTACGCCGCGCCCTGCGTGATCACAGTGCCGAGAATTGGGCCAGCCTCAGTGCTGCAGAACTGATGACAGCCGATCCAA
TCACCGTGGACGCTGATCTGCTCGCAGTCGAAGCGATCAAACAAATGGAATGCAACCGTCGCAAACCCATCTCAGTACTG
CCCGTCGTAGGCCCTGACAGCTCAGGCAATCTGTTGCTCGGACTACTACGGCTTCATGATCTGATTCAAGCAGGGCTGAC
ATGA

Upstream 100 bases:

>100_bases
TTTAATCCCAACCTCTGACGCAACTTGCTGCAGTACCGCCAACAAACCCATTGGCAAACCTGGTCCATCGAATCCAGGCT
CTGGCTTCACCAGACTCTCC

Downstream 100 bases:

>100_bases
GCGGCACTACCCTGATCTCTCGAACCCACCCCCGTTACTGGAACCGAGAGCTGCAATGGCGACTGCAGTGGAAGGCATTA
GCTTCCATCGATCTACTGGT

Product: polysialic acid capsule expression protein KpsF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MAVVCRRITASSRTYSLSALTRCLQEEAAAIAVAAERLSSSQVEKALVLLERCSDQRAKLVITGVGKSGIVARKIAATFS
SIGLMALYLNPLDAMHGDLGVVAQEDVCLLLSNSGETAELLEVLPHLKRRGTARIALVGKPDSSLARGSDVVLEASVDRE
VCPLNLAPTASTAVAMAIGDALAAIWMERRNISPADFAFNHPAGSLGKQLTLTASDLMVPVEKVQPLQPNTSLQEVICKL
TQDGIGSGWVEDPSTAGLLLGLITDGDLRRALRDHSAENWASLSAAELMTADPITVDADLLAVEAIKQMECNRRKPISVL
PVVGPDSSGNLLLGLLRLHDLIQAGLT

Sequences:

>Translated_347_residues
MAVVCRRITASSRTYSLSALTRCLQEEAAAIAVAAERLSSSQVEKALVLLERCSDQRAKLVITGVGKSGIVARKIAATFS
SIGLMALYLNPLDAMHGDLGVVAQEDVCLLLSNSGETAELLEVLPHLKRRGTARIALVGKPDSSLARGSDVVLEASVDRE
VCPLNLAPTASTAVAMAIGDALAAIWMERRNISPADFAFNHPAGSLGKQLTLTASDLMVPVEKVQPLQPNTSLQEVICKL
TQDGIGSGWVEDPSTAGLLLGLITDGDLRRALRDHSAENWASLSAAELMTADPITVDADLLAVEAIKQMECNRRKPISVL
PVVGPDSSGNLLLGLLRLHDLIQAGLT
>Mature_346_residues
AVVCRRITASSRTYSLSALTRCLQEEAAAIAVAAERLSSSQVEKALVLLERCSDQRAKLVITGVGKSGIVARKIAATFSS
IGLMALYLNPLDAMHGDLGVVAQEDVCLLLSNSGETAELLEVLPHLKRRGTARIALVGKPDSSLARGSDVVLEASVDREV
CPLNLAPTASTAVAMAIGDALAAIWMERRNISPADFAFNHPAGSLGKQLTLTASDLMVPVEKVQPLQPNTSLQEVICKLT
QDGIGSGWVEDPSTAGLLLGLITDGDLRRALRDHSAENWASLSAAELMTADPITVDADLLAVEAIKQMECNRRKPISVLP
VVGPDSSGNLLLGLLRLHDLIQAGLT

Specific function: Unknown

COG id: COG0794

COG function: function code M; Predicted sugar phosphate isomerase involved in capsule formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain [H]

Homologues:

Organism=Escherichia coli, GI1789588, Length=290, Percent_Identity=38.2758620689655, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI87082151, Length=311, Percent_Identity=36.9774919614148, Blast_Score=175, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000644
- InterPro:   IPR004800
- InterPro:   IPR001347 [H]

Pfam domain/function: PF00571 CBS; PF01380 SIS [H]

EC number: NA

Molecular weight: Translated: 36667; Mature: 36536

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVCRRITASSRTYSLSALTRCLQEEAAAIAVAAERLSSSQVEKALVLLERCSDQRAKL
CCEEEEEHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VITGVGKSGIVARKIAATFSSIGLMALYLNPLDAMHGDLGVVAQEDVCLLLSNSGETAEL
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCEEECCCEEEEEECCCCHHHH
LEVLPHLKRRGTARIALVGKPDSSLARGSDVVLEASVDREVCPLNLAPTASTAVAMAIGD
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
ALAAIWMERRNISPADFAFNHPAGSLGKQLTLTASDLMVPVEKVQPLQPNTSLQEVICKL
HHHHHHHHHCCCCCCCCEECCCCHHCCCEEEEEHHHHCCCHHHCCCCCCCCHHHHHHHHH
TQDGIGSGWVEDPSTAGLLLGLITDGDLRRALRDHSAENWASLSAAELMTADPITVDADL
HHCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEECHHH
LAVEAIKQMECNRRKPISVLPVVGPDSSGNLLLGLLRLHDLIQAGLT
HHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVVCRRITASSRTYSLSALTRCLQEEAAAIAVAAERLSSSQVEKALVLLERCSDQRAKL
CEEEEEHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VITGVGKSGIVARKIAATFSSIGLMALYLNPLDAMHGDLGVVAQEDVCLLLSNSGETAEL
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCEEECCCEEEEEECCCCHHHH
LEVLPHLKRRGTARIALVGKPDSSLARGSDVVLEASVDREVCPLNLAPTASTAVAMAIGD
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
ALAAIWMERRNISPADFAFNHPAGSLGKQLTLTASDLMVPVEKVQPLQPNTSLQEVICKL
HHHHHHHHHCCCCCCCCEECCCCHHCCCEEEEEHHHHCCCHHHCCCCCCCCHHHHHHHHH
TQDGIGSGWVEDPSTAGLLLGLITDGDLRRALRDHSAENWASLSAAELMTADPITVDADL
HHCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEECHHH
LAVEAIKQMECNRRKPISVLPVVGPDSSGNLLLGLLRLHDLIQAGLT
HHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]