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The map label for this gene is rph

Identifier: 33864097

GI number: 33864097

Start: 1938736

End: 1939470

Strand: Direct

Name: rph

Synonym: PMT1830

Alternate gene names: 33864097

Gene position: 1938736-1939470 (Clockwise)

Preceding gene: 33864093

Following gene: 33864098

Centisome position: 80.42

GC content: 57.01

Gene sequence:

>735_bases
ATGCTCACATCCCCCCAGCCACGTGCTGACGGCCGCCTTGCTGACGTGATGCGGCCCCTTCAGATCACATGGGATCCAAT
GGGCTTTGCCCTCAGTTCGTTAATCATCCGCACTGGTCGCACCTCAGTGCTCTGCAGTGTTTGCGTTGAAGAAGGTGTAC
CCCGCTGGCGCAAAGGCCAAGGCAAAGGCTGGCTCAGCGCCGAGTACCGCCTTCTGCCTGGGTCTACCCCGCAAAGACAG
AACCGTGAACTGCTCAAGCTTTCCGGACGCACTCAAGAAATTCAACGCCTCATTGGCCGCAGCCTGCGCGCCGTGATCGA
CATGGCCGCTCTTGGGGAAACCACCCTGCGAATTGATTGTGACGTCATTCAGGCCGATGCCGGCACACGTACAGCCTCCA
TCACCGGCGCTTGGATTGCCTTAAAACGAGGCTGCGATCGCTTGCTGGAACAAGGGCTGCTCACGCATCAACCCGTCATC
GAGCAAGTTGCTGCAGTATCCGTTGGTTTAGTGGAGAGTTATCCCCTTCTCGACCTCGACTACAGCGAAGACTCCAGGGC
TGATGTGGATCTAAACGTGGTAATGGGCTCCAATGGGCACCTATTGGAGCTGCAGGGCACCGCTGAAGGGGCTCCATTCA
GCCGGAATCAACTCAACGACTTGCTGAATCTGGCAGAACCCGGCCTGCAACATCTACAAGCCTTCCAACGCTCGGCATTA
ATGCAAGAAGACTAA

Upstream 100 bases:

>100_bases
GTAGCGGTGGAAAGCTGCTTAACAAGGCTCTTGACACTATCCATGGTGTAAAAGCACCTGTTTCTGCCAGGCTCAGTTCA
TCTCAACCACCAACGGCACG

Downstream 100 bases:

>100_bases
AAACCGTCACCATTGCTACATGGTAGTGGAGGTCCACTCCCTAACTTCATATTTCTGCCGTTTGCTGCGCAGCCATGGCC
GGCGTCATCCGCGGCTTCAG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MLTSPQPRADGRLADVMRPLQITWDPMGFALSSLIIRTGRTSVLCSVCVEEGVPRWRKGQGKGWLSAEYRLLPGSTPQRQ
NRELLKLSGRTQEIQRLIGRSLRAVIDMAALGETTLRIDCDVIQADAGTRTASITGAWIALKRGCDRLLEQGLLTHQPVI
EQVAAVSVGLVESYPLLDLDYSEDSRADVDLNVVMGSNGHLLELQGTAEGAPFSRNQLNDLLNLAEPGLQHLQAFQRSAL
MQED

Sequences:

>Translated_244_residues
MLTSPQPRADGRLADVMRPLQITWDPMGFALSSLIIRTGRTSVLCSVCVEEGVPRWRKGQGKGWLSAEYRLLPGSTPQRQ
NRELLKLSGRTQEIQRLIGRSLRAVIDMAALGETTLRIDCDVIQADAGTRTASITGAWIALKRGCDRLLEQGLLTHQPVI
EQVAAVSVGLVESYPLLDLDYSEDSRADVDLNVVMGSNGHLLELQGTAEGAPFSRNQLNDLLNLAEPGLQHLQAFQRSAL
MQED
>Mature_244_residues
MLTSPQPRADGRLADVMRPLQITWDPMGFALSSLIIRTGRTSVLCSVCVEEGVPRWRKGQGKGWLSAEYRLLPGSTPQRQ
NRELLKLSGRTQEIQRLIGRSLRAVIDMAALGETTLRIDCDVIQADAGTRTASITGAWIALKRGCDRLLEQGLLTHQPVI
EQVAAVSVGLVESYPLLDLDYSEDSRADVDLNVVMGSNGHLLELQGTAEGAPFSRNQLNDLLNLAEPGLQHLQAFQRSAL
MQED

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=51.9047619047619, Blast_Score=215, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_PROMM (Q7V4V8)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895657.1
- ProteinModelPortal:   Q7V4V8
- SMR:   Q7V4V8
- STRING:   Q7V4V8
- GeneID:   1728121
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1830
- NMPDR:   fig|74547.1.peg.1824
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   PMAR74547:PMT1830-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26783; Mature: 26783

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTSPQPRADGRLADVMRPLQITWDPMGFALSSLIIRTGRTSVLCSVCVEEGVPRWRKGQ
CCCCCCCCCCCCHHHHCCCHHEEECHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHCCC
GKGWLSAEYRLLPGSTPQRQNRELLKLSGRTQEIQRLIGRSLRAVIDMAALGETTLRIDC
CCCEECEEEEECCCCCCCHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
DVIQADAGTRTASITGAWIALKRGCDRLLEQGLLTHQPVIEQVAAVSVGLVESYPLLDLD
EEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEECC
YSEDSRADVDLNVVMGSNGHLLELQGTAEGAPFSRNQLNDLLNLAEPGLQHLQAFQRSAL
CCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MQED
CCCC
>Mature Secondary Structure
MLTSPQPRADGRLADVMRPLQITWDPMGFALSSLIIRTGRTSVLCSVCVEEGVPRWRKGQ
CCCCCCCCCCCCHHHHCCCHHEEECHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHCCC
GKGWLSAEYRLLPGSTPQRQNRELLKLSGRTQEIQRLIGRSLRAVIDMAALGETTLRIDC
CCCEECEEEEECCCCCCCHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
DVIQADAGTRTASITGAWIALKRGCDRLLEQGLLTHQPVIEQVAAVSVGLVESYPLLDLD
EEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEECC
YSEDSRADVDLNVVMGSNGHLLELQGTAEGAPFSRNQLNDLLNLAEPGLQHLQAFQRSAL
CCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MQED
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642