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The map label for this gene is ntcA [H]

Identifier: 33864098

GI number: 33864098

Start: 1939611

End: 1940279

Strand: Direct

Name: ntcA [H]

Synonym: PMT1831

Alternate gene names: 33864098

Gene position: 1939611-1940279 (Clockwise)

Preceding gene: 33864097

Following gene: 33864099

Centisome position: 80.45

GC content: 53.51

Gene sequence:

>669_bases
ATGGCCAACTCGCCTGCTCCACCCCAGAAAACCCTGCTGGAAGTCATCCGTGAACTGGATGGCGCCAGCAACGAAATGGT
GGAGCGTTCCAAAACCATCTTCTTTCCTGGCGATCCTGCCGAAAAGGTTTATCTCATTCGACGCGGCGCGGTTCGGCTAT
CTCGGGTCTATGAATCTGGTGAAGAGATCACCGTGGCATTGCTGCGTGAAAACAGCCTATTTGGAGTGCTCTCTCTACTC
ACAGGCCATCGCTCGGACCGTTTTTATCACTCCGTAGCCTTCACCCGAGTCGAAATGATCACAGCGCCAGCCACCTCAGT
TCGCCAAGCGATCGAAAACGACACCAGCGTGGGGTTACTTCTGCTGCAGGGTCTCTCAAGTCGGATCTTGCAAACCGAGA
CCATGATCGAGACCCTCACTCACCGCGACATGTCATCAAGGCTTGTGAGTTTCCTGCTAGTCCTCTGCCGGGATTTCGGG
GTTCCAGGGGAACAAGGAATCACCATTGACCTGCGCCTCTCACATCAGTCCATCGCTGAAGCAATCGGCTCCACTCGCGT
CACCATTACTCGCCTGTTGGGAGACCTGCGCAATTCAGGTCTAGTGCAAATCGATCGCAAGAAAATCACCGTTCTTGATC
CGATTGCCCTTGCTAAAAAATTCAACTGA

Upstream 100 bases:

>100_bases
CTAACTTCATATTTCTGCCGTTTGCTGCGCAGCCATGGCCGGCGTCATCCGCGGCTTCAGCCGTTACGCCCAGCAACCCT
ATGTTCAAGGGTCAGGAAGT

Downstream 100 bases:

>100_bases
TCCCCGTCCCAGTCCAGGATGGTGAAACGGGTTGACACTATTCCTGATCCCACCCGCCCATTGTCGTGACCGGCTGGCTG
CTGATCCTCTCCCTACTCGT

Product: CRP family global nitrogen regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MANSPAPPQKTLLEVIRELDGASNEMVERSKTIFFPGDPAEKVYLIRRGAVRLSRVYESGEEITVALLRENSLFGVLSLL
TGHRSDRFYHSVAFTRVEMITAPATSVRQAIENDTSVGLLLLQGLSSRILQTETMIETLTHRDMSSRLVSFLLVLCRDFG
VPGEQGITIDLRLSHQSIAEAIGSTRVTITRLLGDLRNSGLVQIDRKKITVLDPIALAKKFN

Sequences:

>Translated_222_residues
MANSPAPPQKTLLEVIRELDGASNEMVERSKTIFFPGDPAEKVYLIRRGAVRLSRVYESGEEITVALLRENSLFGVLSLL
TGHRSDRFYHSVAFTRVEMITAPATSVRQAIENDTSVGLLLLQGLSSRILQTETMIETLTHRDMSSRLVSFLLVLCRDFG
VPGEQGITIDLRLSHQSIAEAIGSTRVTITRLLGDLRNSGLVQIDRKKITVLDPIALAKKFN
>Mature_221_residues
ANSPAPPQKTLLEVIRELDGASNEMVERSKTIFFPGDPAEKVYLIRRGAVRLSRVYESGEEITVALLRENSLFGVLSLLT
GHRSDRFYHSVAFTRVEMITAPATSVRQAIENDTSVGLLLLQGLSSRILQTETMIETLTHRDMSSRLVSFLLVLCRDFGV
PGEQGITIDLRLSHQSIAEAIGSTRVTITRLLGDLRNSGLVQIDRKKITVLDPIALAKKFN

Specific function: Required for full expression of proteins subject to ammonium repression. Transcriptional activator of genes subject to nitrogen control [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=200, Percent_Identity=24, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24605; Mature: 24474

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSPAPPQKTLLEVIRELDGASNEMVERSKTIFFPGDPAEKVYLIRRGAVRLSRVYESG
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCEEEECCCCHHHEEEEHHHHHHHHHHHHCC
EEITVALLRENSLFGVLSLLTGHRSDRFYHSVAFTRVEMITAPATSVRQAIENDTSVGLL
CEEEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHH
LLQGLSSRILQTETMIETLTHRDMSSRLVSFLLVLCRDFGVPGEQGITIDLRLSHQSIAE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHH
AIGSTRVTITRLLGDLRNSGLVQIDRKKITVLDPIALAKKFN
HHCCCHHHHHHHHHHHCCCCEEEECCCEEEEECHHHHHHCCC
>Mature Secondary Structure 
ANSPAPPQKTLLEVIRELDGASNEMVERSKTIFFPGDPAEKVYLIRRGAVRLSRVYESG
CCCCCCCHHHHHHHHHHHCCCCHHHHHHCCEEEECCCCHHHEEEEHHHHHHHHHHHHCC
EEITVALLRENSLFGVLSLLTGHRSDRFYHSVAFTRVEMITAPATSVRQAIENDTSVGLL
CEEEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHH
LLQGLSSRILQTETMIETLTHRDMSSRLVSFLLVLCRDFGVPGEQGITIDLRLSHQSIAE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHH
AIGSTRVTITRLLGDLRNSGLVQIDRKKITVLDPIALAKKFN
HHCCCHHHHHHHHHHHCCCCEEEECCCEEEEECHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8366058; 8905231 [H]