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The map label for this gene is gidB [H]

Identifier: 33863763

GI number: 33863763

Start: 1579977

End: 1580720

Strand: Direct

Name: gidB [H]

Synonym: PMT1496

Alternate gene names: 33863763

Gene position: 1579977-1580720 (Clockwise)

Preceding gene: 33863760

Following gene: 33863777

Centisome position: 65.54

GC content: 58.47

Gene sequence:

>744_bases
ATGTCAAATCAGGCCTGCTTTAGCAACCCAGGCCCGGAGCTCTGGAATGCCCTTGGATGGCATCCCTCCAGTGAACAACT
GGAGCAAATGATCGCCCTACAAGCCCTCCTACGCCAATGGAATGCCCGGGTCAACCTCACCCGCCTGGTCGAAGGTGGTG
ACTACTGGATCATGCAGGTGTTCGACAGTCTCTGGCCTCTGCAGAGCGAATTGCAGAACGCCCAACAACCACGTCGATGC
ATCGATATCGGCAGCGGCGGTGGCTTTCCCGGTCTGGTGCTAGCCATCGCACTACCAGGCGCAAGGATCACCTTGGTGGA
TTCGGTAGGCCGCAAGACAGCTGCCCTCAAGGCAATGGCGGCAAAGCTAGGCCTAACATCCCGCGTCACGGTACGTAGCG
AACGGGCAGAATTCACCGGGCAGGATCCCTGCTGCCGCGGCTTATTTGATCTCGCGATGGCTCGCGCAGTGAGCACAGCA
CCGGTTGTCGCCGAATACCTCGTCCCCCTTCTCAAGCCAAGCGGTGAAGCCCTGCTATTTCGTGGCCATTGGAGCCCCAA
CGATGCAAAGGATCTCGCTAAGGCTCTCAGATTGCTACAAGCCGATCTAATCAAGATGGAACGTCGCGAACTTCCTGACA
ATCGCGGGGTGCGTCACCAATTACGCCTGCGCGCAACGCTCCCCTGCCCAGCAACCTTTCCACGTCCAATCGGTGTACCT
GCCAAGAACCCGCTCGGGTCTTAA

Upstream 100 bases:

>100_bases
CGCAAGAGTCAAAACCAGAAGTTGATCGGCAACTCGCAGAACATCATCTCCTTTCCCCGTGAACAATTCCATCCTAGAGA
GAACTATGTTCTGATTGCCC

Downstream 100 bases:

>100_bases
ACACTGGTCAGTCGCTGCTGAGGGTGACCACCAAGACGGTTGCGCAACCTACGCAGCAAGCCTGGGATCTGATCACACCA
GGGGCTGTTCACCAACACCT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSNQACFSNPGPELWNALGWHPSSEQLEQMIALQALLRQWNARVNLTRLVEGGDYWIMQVFDSLWPLQSELQNAQQPRRC
IDIGSGGGFPGLVLAIALPGARITLVDSVGRKTAALKAMAAKLGLTSRVTVRSERAEFTGQDPCCRGLFDLAMARAVSTA
PVVAEYLVPLLKPSGEALLFRGHWSPNDAKDLAKALRLLQADLIKMERRELPDNRGVRHQLRLRATLPCPATFPRPIGVP
AKNPLGS

Sequences:

>Translated_247_residues
MSNQACFSNPGPELWNALGWHPSSEQLEQMIALQALLRQWNARVNLTRLVEGGDYWIMQVFDSLWPLQSELQNAQQPRRC
IDIGSGGGFPGLVLAIALPGARITLVDSVGRKTAALKAMAAKLGLTSRVTVRSERAEFTGQDPCCRGLFDLAMARAVSTA
PVVAEYLVPLLKPSGEALLFRGHWSPNDAKDLAKALRLLQADLIKMERRELPDNRGVRHQLRLRATLPCPATFPRPIGVP
AKNPLGS
>Mature_246_residues
SNQACFSNPGPELWNALGWHPSSEQLEQMIALQALLRQWNARVNLTRLVEGGDYWIMQVFDSLWPLQSELQNAQQPRRCI
DIGSGGGFPGLVLAIALPGARITLVDSVGRKTAALKAMAAKLGLTSRVTVRSERAEFTGQDPCCRGLFDLAMARAVSTAP
VVAEYLVPLLKPSGEALLFRGHWSPNDAKDLAKALRLLQADLIKMERRELPDNRGVRHQLRLRATLPCPATFPRPIGVPA
KNPLGS

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=137, Percent_Identity=30.6569343065693, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: 2.1.-.-

Molecular weight: Translated: 27078; Mature: 26947

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQACFSNPGPELWNALGWHPSSEQLEQMIALQALLRQWNARVNLTRLVEGGDYWIMQV
CCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHH
FDSLWPLQSELQNAQQPRRCIDIGSGGGFPGLVLAIALPGARITLVDSVGRKTAALKAMA
HHHHCCHHHHHHHHHCCHHHEECCCCCCHHHHHHHHHCCCCEEEEEHHHCHHHHHHHHHH
AKLGLTSRVTVRSERAEFTGQDPCCRGLFDLAMARAVSTAPVVAEYLVPLLKPSGEALLF
HHHCCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
RGHWSPNDAKDLAKALRLLQADLIKMERRELPDNRGVRHQLRLRATLPCPATFPRPIGVP
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCC
AKNPLGS
CCCCCCC
>Mature Secondary Structure 
SNQACFSNPGPELWNALGWHPSSEQLEQMIALQALLRQWNARVNLTRLVEGGDYWIMQV
CCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHH
FDSLWPLQSELQNAQQPRRCIDIGSGGGFPGLVLAIALPGARITLVDSVGRKTAALKAMA
HHHHCCHHHHHHHHHCCHHHEECCCCCCHHHHHHHHHCCCCEEEEEHHHCHHHHHHHHHH
AKLGLTSRVTVRSERAEFTGQDPCCRGLFDLAMARAVSTAPVVAEYLVPLLKPSGEALLF
HHHCCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
RGHWSPNDAKDLAKALRLLQADLIKMERRELPDNRGVRHQLRLRATLPCPATFPRPIGVP
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCC
AKNPLGS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA