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The map label for this gene is menD [H]

Identifier: 33862679

GI number: 33862679

Start: 456220

End: 457968

Strand: Reverse

Name: menD [H]

Synonym: PMT0406

Alternate gene names: 33862679

Gene position: 457968-456220 (Counterclockwise)

Preceding gene: 33862682

Following gene: 33862678

Centisome position: 19.0

GC content: 55.23

Gene sequence:

>1749_bases
TTGGGGCTGCGCCATCTCGTGCTTTGTCCAGGCAGCCGCTCGGGTCCCCTTGCACTGGCTGCAGGAGGAATGGCACGTAC
CAATCGCTTGCGGCTTAGCACTGCCATTGATGAGCGCTCTGCTGCTTTCTTGGCTTTGGGTTTTAGCACTGCTACAGGCA
CTGCAGCAGCTGTAGTGACCACTTCGGGCACCGCTGTAGCCAACCTTTTGCCTGCAGCGGTGGAGGCTGATCGATCCTGT
CAGCCCCTGTTGCTGCTCACTGCTGACCGCCCCTATCGACTCAAAGACTGCGGTGCCAATCAAACGGTGAATCAGGAGAC
TTTCTTGAGTCCTGCCTGTCGATGGATTGGGCAGGGCCCAAGAGAGGGGTTGCATCTTTTCAGTAAAGAAACGCTGGAAT
CATTCGCAGAGAAGGCCTGGCAACGAGCTCATCACCCCGCAGGGGCCGTTCATCTCAATCTGCCTTTTGAGGAACCTCTA
CATCTCAGTGAAGAAGAGCAGAGGATGATCTGGAAGGGGTGGTCTCCCAAGATTCCACGTTCATCTCCGATCAAGCCAAT
CAATCTGGCAATGGCAGCTGAGGGGACTAATGGGGTTACCGATCAGGCTCCCTTTGCATTGGATCCTTTACGACCCGGCG
TTGTGATAGCTGGTGCTTGGCGAGGCCTAAGCAAAGACCTCTTTGCTTTTCAGCAATCCTTGCGGGAGTGGCAGGCCTTG
AGTGGCTGGCCCGTGCTGGCCGATCCACTATCGGCTCTGCCTTCAGACCAACCTGGTCTAATCCGCTCCTGGGAGCTGCT
TTTGGCTACTGGGCTTTTAGGCTCTCAGGAGCAGCTTCAAGTGCTGCGTCTAGGACCAATGTCAGCCAGTCGGAGCCTTG
AGGCATGGTTGAAAAGCTTTGGTGACGGTCAGCTCCTGATTACAGAGGGAGACTCCCGTTGTCTCGATCCCCTTGGTTTG
TCAGTGCAATGGAATCATGGATTGACGAGCTGGTGGCAACACCATCACCATCGCTGGATCGATGCTGATGCAGCCTCTCA
ACAGGCAACCAAGGCTCTGCTGAGGAAATGGCAAATCAGCGATCGTTTTGCTCAGGAGTGGCTGGATCAGCAGTTACCCC
TGCAGGGTGCCATCACAGAACCAGCCCTGGCTCGCTGGTTATCCCGTCTTCTGCCAACTGAGCTACCAATCATGTTGGCA
GCGAGTAGTCCTGTGAGGGACTGGCTGGCTTATGCGGACAAGAGCTTATTTTCGAGACGATGCTTCAGTTTTCGAGGCGC
GTCTGGTATCGACGGCACCCTTTCTTTAAGCATGGGTTTAGCGATGGCTCTTGGGCCCACTTTGCTTGTAAGCGGCGACC
TGGCTCTCCTTCATGACAGCAATGGCTGGTTGCTTGCTCATCCCCAGCGCCCGCCTTTGGTGGTGGTGTTGATCGACAAT
GGTGGCGGAGGCATCTTCGAGCAACTTCTGGTGAAGACAGCTCCTAGCGAAGCATTTGAGCAGCTGTTTGCCATGCCTCA
GGAGGTTGACCCTCTTGCTCTTGCAGGGGCTCATAACATTCCTCACCGTCAGGTGGCCTGTCTCGAAGACCTACCAGCAG
CCTTGGAGTGGGGGCTCTTCCAGGCTGGACCTGTTTTGATACGTGTGTGCACGCATCGGCGTCAAGATTCATCGATGAGG
CAACAGCTTCGCGAGGGATTGATGATGCATCTGCAGTCGATTTCCCAAAATGGCCACATTGATCTTTGA

Upstream 100 bases:

>100_bases
TCGGTATTGACCGAGAATCGCATTGGCTAAGGCTCAAGCTCTGTCTCTTGCTCACAACAATCTGTTGGTTTGCCTGAGCT
TGCTCGAGGCATTTCATGCC

Downstream 100 bases:

>100_bases
TCCCGATGGCTATGGATTCCAATCGGTTTGATAGGAGGCCAAGACAGGTTTTGCCTGGCAAGCCCACGATCGAATGGGAA
CCCTGGGGTGAATATCAAGA

Product: menaquinone biosynthesis protein; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MGLRHLVLCPGSRSGPLALAAGGMARTNRLRLSTAIDERSAAFLALGFSTATGTAAAVVTTSGTAVANLLPAAVEADRSC
QPLLLLTADRPYRLKDCGANQTVNQETFLSPACRWIGQGPREGLHLFSKETLESFAEKAWQRAHHPAGAVHLNLPFEEPL
HLSEEEQRMIWKGWSPKIPRSSPIKPINLAMAAEGTNGVTDQAPFALDPLRPGVVIAGAWRGLSKDLFAFQQSLREWQAL
SGWPVLADPLSALPSDQPGLIRSWELLLATGLLGSQEQLQVLRLGPMSASRSLEAWLKSFGDGQLLITEGDSRCLDPLGL
SVQWNHGLTSWWQHHHHRWIDADAASQQATKALLRKWQISDRFAQEWLDQQLPLQGAITEPALARWLSRLLPTELPIMLA
ASSPVRDWLAYADKSLFSRRCFSFRGASGIDGTLSLSMGLAMALGPTLLVSGDLALLHDSNGWLLAHPQRPPLVVVLIDN
GGGGIFEQLLVKTAPSEAFEQLFAMPQEVDPLALAGAHNIPHRQVACLEDLPAALEWGLFQAGPVLIRVCTHRRQDSSMR
QQLREGLMMHLQSISQNGHIDL

Sequences:

>Translated_582_residues
MGLRHLVLCPGSRSGPLALAAGGMARTNRLRLSTAIDERSAAFLALGFSTATGTAAAVVTTSGTAVANLLPAAVEADRSC
QPLLLLTADRPYRLKDCGANQTVNQETFLSPACRWIGQGPREGLHLFSKETLESFAEKAWQRAHHPAGAVHLNLPFEEPL
HLSEEEQRMIWKGWSPKIPRSSPIKPINLAMAAEGTNGVTDQAPFALDPLRPGVVIAGAWRGLSKDLFAFQQSLREWQAL
SGWPVLADPLSALPSDQPGLIRSWELLLATGLLGSQEQLQVLRLGPMSASRSLEAWLKSFGDGQLLITEGDSRCLDPLGL
SVQWNHGLTSWWQHHHHRWIDADAASQQATKALLRKWQISDRFAQEWLDQQLPLQGAITEPALARWLSRLLPTELPIMLA
ASSPVRDWLAYADKSLFSRRCFSFRGASGIDGTLSLSMGLAMALGPTLLVSGDLALLHDSNGWLLAHPQRPPLVVVLIDN
GGGGIFEQLLVKTAPSEAFEQLFAMPQEVDPLALAGAHNIPHRQVACLEDLPAALEWGLFQAGPVLIRVCTHRRQDSSMR
QQLREGLMMHLQSISQNGHIDL
>Mature_581_residues
GLRHLVLCPGSRSGPLALAAGGMARTNRLRLSTAIDERSAAFLALGFSTATGTAAAVVTTSGTAVANLLPAAVEADRSCQ
PLLLLTADRPYRLKDCGANQTVNQETFLSPACRWIGQGPREGLHLFSKETLESFAEKAWQRAHHPAGAVHLNLPFEEPLH
LSEEEQRMIWKGWSPKIPRSSPIKPINLAMAAEGTNGVTDQAPFALDPLRPGVVIAGAWRGLSKDLFAFQQSLREWQALS
GWPVLADPLSALPSDQPGLIRSWELLLATGLLGSQEQLQVLRLGPMSASRSLEAWLKSFGDGQLLITEGDSRCLDPLGLS
VQWNHGLTSWWQHHHHRWIDADAASQQATKALLRKWQISDRFAQEWLDQQLPLQGAITEPALARWLSRLLPTELPIMLAA
SSPVRDWLAYADKSLFSRRCFSFRGASGIDGTLSLSMGLAMALGPTLLVSGDLALLHDSNGWLLAHPQRPPLVVVLIDNG
GGGIFEQLLVKTAPSEAFEQLFAMPQEVDPLALAGAHNIPHRQVACLEDLPAALEWGLFQAGPVLIRVCTHRRQDSSMRQ
QLREGLMMHLQSISQNGHIDL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=524, Percent_Identity=31.1068702290076, Blast_Score=162, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 63529; Mature: 63397

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLRHLVLCPGSRSGPLALAAGGMARTNRLRLSTAIDERSAAFLALGFSTATGTAAAVVT
CCCCEEEECCCCCCCCEEEEECCCCCCCEEEEEEHHCCCCCEEEEEECCCCCCCEEEEEE
TSGTAVANLLPAAVEADRSCQPLLLLTADRPYRLKDCGANQTVNQETFLSPACRWIGQGP
ECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCC
REGLHLFSKETLESFAEKAWQRAHHPAGAVHLNLPFEEPLHLSEEEQRMIWKGWSPKIPR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHEECCCCCCCCC
SSPIKPINLAMAAEGTNGVTDQAPFALDPLRPGVVIAGAWRGLSKDLFAFQQSLREWQAL
CCCCCCEEEEEEECCCCCCCCCCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
SGWPVLADPLSALPSDQPGLIRSWELLLATGLLGSQEQLQVLRLGPMSASRSLEAWLKSF
CCCCCHHCHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHEEEECCCCCHHHHHHHHHHC
GDGQLLITEGDSRCLDPLGLSVQWNHGLTSWWQHHHHRWIDADAASQQATKALLRKWQIS
CCCEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHH
DRFAQEWLDQQLPLQGAITEPALARWLSRLLPTELPIMLAASSPVRDWLAYADKSLFSRR
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
CFSFRGASGIDGTLSLSMGLAMALGPTLLVSGDLALLHDSNGWLLAHPQRPPLVVVLIDN
HHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCEEEEECCCCEEEECCCCCCEEEEEEEC
GGGGIFEQLLVKTAPSEAFEQLFAMPQEVDPLALAGAHNIPHRQVACLEDLPAALEWGLF
CCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHCHHHHHCCCC
QAGPVLIRVCTHRRQDSSMRQQLREGLMMHLQSISQNGHIDL
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GLRHLVLCPGSRSGPLALAAGGMARTNRLRLSTAIDERSAAFLALGFSTATGTAAAVVT
CCCEEEECCCCCCCCEEEEECCCCCCCEEEEEEHHCCCCCEEEEEECCCCCCCEEEEEE
TSGTAVANLLPAAVEADRSCQPLLLLTADRPYRLKDCGANQTVNQETFLSPACRWIGQGP
ECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCC
REGLHLFSKETLESFAEKAWQRAHHPAGAVHLNLPFEEPLHLSEEEQRMIWKGWSPKIPR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHEECCCCCCCCC
SSPIKPINLAMAAEGTNGVTDQAPFALDPLRPGVVIAGAWRGLSKDLFAFQQSLREWQAL
CCCCCCEEEEEEECCCCCCCCCCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
SGWPVLADPLSALPSDQPGLIRSWELLLATGLLGSQEQLQVLRLGPMSASRSLEAWLKSF
CCCCCHHCHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHEEEECCCCCHHHHHHHHHHC
GDGQLLITEGDSRCLDPLGLSVQWNHGLTSWWQHHHHRWIDADAASQQATKALLRKWQIS
CCCEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHH
DRFAQEWLDQQLPLQGAITEPALARWLSRLLPTELPIMLAASSPVRDWLAYADKSLFSRR
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
CFSFRGASGIDGTLSLSMGLAMALGPTLLVSGDLALLHDSNGWLLAHPQRPPLVVVLIDN
HHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCEEEEECCCCEEEECCCCCCEEEEEEEC
GGGGIFEQLLVKTAPSEAFEQLFAMPQEVDPLALAGAHNIPHRQVACLEDLPAALEWGLF
CCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHCHHHHHCCCC
QAGPVLIRVCTHRRQDSSMRQQLREGLMMHLQSISQNGHIDL
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA