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The map label for this gene is smpB [H]

Identifier: 33862428

GI number: 33862428

Start: 174397

End: 174894

Strand: Reverse

Name: smpB [H]

Synonym: PMT0155

Alternate gene names: 33862428

Gene position: 174894-174397 (Counterclockwise)

Preceding gene: 33862434

Following gene: 33862425

Centisome position: 7.25

GC content: 55.42

Gene sequence:

>498_bases
ATGGCCAAGGGAGGAAACAAAAAAGCGACTGCTGCTGCTCGAGCCGCGGCCAACCGTCTACTCGCAGACAACCGACTGGC
AAGGCATCAATACGAGATCCTCGACACCCTCGAAACTGGCATTGAGTTGGTGGGAACCGAAGTCAAGTCAATCCGGGCCG
GCCAAGCCAATCTCCGCGATGGCTTCTGCCTGATTCGCAAGGGCGAACTCCAGCTCCACAATGTTCATATCTCCCCTCAT
AGCCATGCCGGCAGCTACTTCAATCACGATCCCCTACGAACACGCAAACTGCTCGCGCACCGCCGTGAGATCGATAAACT
TCGCGGCCAACTCGACAGAAAAGGGCTCACACTGATTCCCCTCAACCTTCATCTCAAGGGTTCATGGATCAAACTCACGA
TTGGGCTGGGCAAAGGTCGCAAACTGCACGACAAACGCCAAGACGAGAAACGCAAACAAGCCGATCGAGAAGTGAAGTCG
GCTCTGGCGCGCTACTGA

Upstream 100 bases:

>100_bases
CTGGCGGCTGGGAATCAGCTCGGCCAGCACTGGAAGACACAATCGCCATGTTGGAAGAGTACCGATCTCTGGATAGTGTC
GAAAGCATCGGTAAACGCAG

Downstream 100 bases:

>100_bases
TCAGCCAAGGGGGTCAAGGAGGCTGAAAAAGTTTTCTTTCGCCGAAGCACGAGGCGAGGCAATCTCGGTATTCTAAAAAG
GTGTTCTGACCGACCAAAGA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MAKGGNKKATAAARAAANRLLADNRLARHQYEILDTLETGIELVGTEVKSIRAGQANLRDGFCLIRKGELQLHNVHISPH
SHAGSYFNHDPLRTRKLLAHRREIDKLRGQLDRKGLTLIPLNLHLKGSWIKLTIGLGKGRKLHDKRQDEKRKQADREVKS
ALARY

Sequences:

>Translated_165_residues
MAKGGNKKATAAARAAANRLLADNRLARHQYEILDTLETGIELVGTEVKSIRAGQANLRDGFCLIRKGELQLHNVHISPH
SHAGSYFNHDPLRTRKLLAHRREIDKLRGQLDRKGLTLIPLNLHLKGSWIKLTIGLGKGRKLHDKRQDEKRKQADREVKS
ALARY
>Mature_164_residues
AKGGNKKATAAARAAANRLLADNRLARHQYEILDTLETGIELVGTEVKSIRAGQANLRDGFCLIRKGELQLHNVHISPHS
HAGSYFNHDPLRTRKLLAHRREIDKLRGQLDRKGLTLIPLNLHLKGSWIKLTIGLGKGRKLHDKRQDEKRKQADREVKSA
LARY

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=138, Percent_Identity=45.6521739130435, Blast_Score=130, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18615; Mature: 18484

Theoretical pI: Translated: 11.19; Mature: 11.19

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGGNKKATAAARAAANRLLADNRLARHQYEILDTLETGIELVGTEVKSIRAGQANLRD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GFCLIRKGELQLHNVHISPHSHAGSYFNHDPLRTRKLLAHRREIDKLRGQLDRKGLTLIP
CEEEEEECCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
LNLHLKGSWIKLTIGLGKGRKLHDKRQDEKRKQADREVKSALARY
EEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKGGNKKATAAARAAANRLLADNRLARHQYEILDTLETGIELVGTEVKSIRAGQANLRD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GFCLIRKGELQLHNVHISPHSHAGSYFNHDPLRTRKLLAHRREIDKLRGQLDRKGLTLIP
CEEEEEECCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
LNLHLKGSWIKLTIGLGKGRKLHDKRQDEKRKQADREVKSALARY
EEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA