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The map label for this gene is dus2 [H]

Identifier: 33862288

GI number: 33862288

Start: 17842

End: 18861

Strand: Reverse

Name: dus2 [H]

Synonym: PMT0015

Alternate gene names: 33862288

Gene position: 18861-17842 (Counterclockwise)

Preceding gene: 33862291

Following gene: 33862280

Centisome position: 0.78

GC content: 53.04

Gene sequence:

>1020_bases
ATGCAGCATGAATCAATGATTGCTCCTTTCACGCCACAGGTTGATGGTGCTTATCGGTTCAGCGTGGCTCCAATGCTCGA
CTGCACAGATCGACACTTCAGGGTACTAATGCGACAAATCAGTCGCCGGGCGTTGCTGTACACGGAAATGTTGGTGGCCC
AAGCTCTGCATCACAGCAACCGTCTTGATCATCTGCTCGATTTCGACATCATCGAGCATCCCTTGTCTCTACAAGTAGGA
GGCGATGATCCAAAAATACTTGCAGAAGCAGCGCGTCTGGCCGATGCCTGGGGCTACGACGAAATCAACCTCAACGTGGG
ATGTCCCAGCTCAAGAGCAAAAGCAGGCAACTTCGGTGCCTGCCTAATGGCTAAACCTGATCAAGTCGCACGTTGTGTTG
AAGCGATGGCGATGGCGAGCCCTCTTCCAGTCACTGTGAAACACCGTCTAGGAATTGATAATTTCGATAGCGACGCTCTA
CTCATGACCTTTGTCGACCGAGTGTCCATAGCAGGAGCCACTCGCTTTACTGTGCATGCACGAAAAGCCTGGCTAGAAGG
GCTTGACCCCAAACAAAACCGCACGATTCCACCACTTCAACATCAACGAGTCACCCATCTCAAGCAACAACGCCCGCAGC
TCACTATTGAAATCAATGGAGGGCTAGAACACCCTGCCGACTGCCTAACAGCGCTGCAAACCTGTGATGGGGCAATGGTG
GGGCGAGCAGCGTATGCGCATCCGCTCCGCTGGCAGAGCATGGATGAGCTGGTCTATGGAGAACAACCACGCTCAATCAA
TGCTTCTCAAGTCATAGGAGGATTACTCCCTCATGCCGAAACCCACCTAAGCCGAGGTGGCCGGCTATGGGATCTTTGCC
GACATCTTTTACAACTCGTTGAAGGGGTACCGGGCGCCAAAGCCTGGCGGCGAGACCTTGGCATCAAGGCTCAAAAAGTC
GATGCCGATCTAAGAGTGCTGCAAAAAGCAGCCCAGCAACTTGAAGATGCCGGGCTTTAA

Upstream 100 bases:

>100_bases
CTATCAGCAGAGAACGACGACTCAATAGCAAAGCAGTAATGGGCATAAGCGCAGCCAAAATCAATGACCTTCTTTCTAGG
AAAGCGCAAAACAACTATGA

Downstream 100 bases:

>100_bases
GGACTTAACTCAGCCCTCCGCTCCCCCATATCCGCTGTAGCTGTCACCACCTTCACCCTCAGGTGCAGCGCCTCCCCTAC
GACGACGAGTGCGACCCTCG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MQHESMIAPFTPQVDGAYRFSVAPMLDCTDRHFRVLMRQISRRALLYTEMLVAQALHHSNRLDHLLDFDIIEHPLSLQVG
GDDPKILAEAARLADAWGYDEINLNVGCPSSRAKAGNFGACLMAKPDQVARCVEAMAMASPLPVTVKHRLGIDNFDSDAL
LMTFVDRVSIAGATRFTVHARKAWLEGLDPKQNRTIPPLQHQRVTHLKQQRPQLTIEINGGLEHPADCLTALQTCDGAMV
GRAAYAHPLRWQSMDELVYGEQPRSINASQVIGGLLPHAETHLSRGGRLWDLCRHLLQLVEGVPGAKAWRRDLGIKAQKV
DADLRVLQKAAQQLEDAGL

Sequences:

>Translated_339_residues
MQHESMIAPFTPQVDGAYRFSVAPMLDCTDRHFRVLMRQISRRALLYTEMLVAQALHHSNRLDHLLDFDIIEHPLSLQVG
GDDPKILAEAARLADAWGYDEINLNVGCPSSRAKAGNFGACLMAKPDQVARCVEAMAMASPLPVTVKHRLGIDNFDSDAL
LMTFVDRVSIAGATRFTVHARKAWLEGLDPKQNRTIPPLQHQRVTHLKQQRPQLTIEINGGLEHPADCLTALQTCDGAMV
GRAAYAHPLRWQSMDELVYGEQPRSINASQVIGGLLPHAETHLSRGGRLWDLCRHLLQLVEGVPGAKAWRRDLGIKAQKV
DADLRVLQKAAQQLEDAGL
>Mature_339_residues
MQHESMIAPFTPQVDGAYRFSVAPMLDCTDRHFRVLMRQISRRALLYTEMLVAQALHHSNRLDHLLDFDIIEHPLSLQVG
GDDPKILAEAARLADAWGYDEINLNVGCPSSRAKAGNFGACLMAKPDQVARCVEAMAMASPLPVTVKHRLGIDNFDSDAL
LMTFVDRVSIAGATRFTVHARKAWLEGLDPKQNRTIPPLQHQRVTHLKQQRPQLTIEINGGLEHPADCLTALQTCDGAMV
GRAAYAHPLRWQSMDELVYGEQPRSINASQVIGGLLPHAETHLSRGGRLWDLCRHLLQLVEGVPGAKAWRRDLGIKAQKV
DADLRVLQKAAQQLEDAGL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=247, Percent_Identity=27.1255060728745, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI40807366, Length=166, Percent_Identity=32.5301204819277, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI145693211, Length=329, Percent_Identity=47.112462006079, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI1788462, Length=266, Percent_Identity=27.4436090225564, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789660, Length=300, Percent_Identity=27, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=211, Percent_Identity=28.9099526066351, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19921524, Length=256, Percent_Identity=26.5625, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37594; Mature: 37594

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHESMIAPFTPQVDGAYRFSVAPMLDCTDRHFRVLMRQISRRALLYTEMLVAQALHHSN
CCCCCCCCCCCCCCCCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RLDHLLDFDIIEHPLSLQVGGDDPKILAEAARLADAWGYDEINLNVGCPSSRAKAGNFGA
HHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCE
CLMAKPDQVARCVEAMAMASPLPVTVKHRLGIDNFDSDALLMTFVDRVSIAGATRFTVHA
EEEECHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHH
RKAWLEGLDPKQNRTIPPLQHQRVTHLKQQRPQLTIEINGGLEHPADCLTALQTCDGAMV
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCHHH
GRAAYAHPLRWQSMDELVYGEQPRSINASQVIGGLLPHAETHLSRGGRLWDLCRHLLQLV
HHHHHCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHH
EGVPGAKAWRRDLGIKAQKVDADLRVLQKAAQQLEDAGL
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQHESMIAPFTPQVDGAYRFSVAPMLDCTDRHFRVLMRQISRRALLYTEMLVAQALHHSN
CCCCCCCCCCCCCCCCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RLDHLLDFDIIEHPLSLQVGGDDPKILAEAARLADAWGYDEINLNVGCPSSRAKAGNFGA
HHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCE
CLMAKPDQVARCVEAMAMASPLPVTVKHRLGIDNFDSDALLMTFVDRVSIAGATRFTVHA
EEEECHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHH
RKAWLEGLDPKQNRTIPPLQHQRVTHLKQQRPQLTIEINGGLEHPADCLTALQTCDGAMV
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCHHH
GRAAYAHPLRWQSMDELVYGEQPRSINASQVIGGLLPHAETHLSRGGRLWDLCRHLLQLV
HHHHHCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHH
EGVPGAKAWRRDLGIKAQKVDADLRVLQKAAQQLEDAGL
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]