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The map label for this gene is proS

Identifier: 32267225

GI number: 32267225

Start: 1651425

End: 1653191

Strand: Direct

Name: proS

Synonym: HH1726

Alternate gene names: 32267225

Gene position: 1651425-1653191 (Clockwise)

Preceding gene: 32267224

Following gene: 32267226

Centisome position: 91.79

GC content: 36.96

Gene sequence:

>1767_bases
ATGAGGTTTTCACAACTTTTTGTAAATACTCTTAAAGAATCTCCTAAAGATGCAGTGCTTAAAAGTCATCAATATCTTAT
TCGTGGGGGCTTTATACAGCAAATCGGCAGTGGTATTTATAATTTTTTACCCCTTGGCAAAAAACTCCTTGATAAAGTGC
GCTTTATTGTTAAAGAAGAAATGGACAAGAGTGGTGCACAAGAGATTCTTATGGGATTTGTTACTCCTGCAGAGCTATGG
AGAGAATCTGGGCGTTATGAACAATATGGCAGGGAGCTTTTGCGTTTTGTAGATAGAAAAGAGAATGAATTTGTGCTTGG
ACCTACGCACGAGGAAGTCATAACGCATATTGCAAAAAATACGATTAAAAGCTATAAGCAACTCCCGCTTCATCTTTATC
AGATTCATAGTAAATTTCGTGATGAACTGCGTCCGCGATTTGGACTTATGCGTGGGCGTGAGTTTATTATGAAAGATGGA
TATAGTTTTCATAGTAATTATGCAGATTTGAATAGAGAGTTTGATGTTATGGAGGCGACATATAAAAGGATTTTGCAAAG
AATGGGCTTAGAGTTTAAAGTTGTAGAGGCAGATTCTGGAGCAATTGGTGGAAGCGGAAGTAAAGAATTTATGGTATTAG
CGCCTTGCGGAGAGGATACTATTGTCGTATGTAAGGGTTGTGAATATGGTGCAAATATTGAAGCAAGTAAAAGAGCGCCA
CGCACTGCTCCGCGTCCAAATGAAATAAAATATGATAGCAATGCACCCCAGGCAGCTTTTGCACGATTTTTTACTCCTGA
TATAAAGAATATAGAATCTTTAAGCGCATTTTTTAAGGTTGATAAGTTTTGGACAATCAAAGCAATAGTAAAAAAAGCAA
TCAAAGCAAATAATGAAAGTGAATTGGTGTATTTTTTTGTGCGTGGTGATGATGAGGGTGAAGAAACAAAAATGCTCAAT
GCCATAAATAAACATACAAATTGTTATCTTGCCTTAGAAGATGCGAGTGTAGAGGAGATTCAAGCAGCTGGATTGGAAGT
AGGATTTATTGGGGCTTATGGGTTGCGTCATATTACTCAAGCTACACATATCTATTTTGATGAGAGTTTAAGGGACGCCT
CAAATCTCATCTGCGGAGCAAATGAGAAGGATTATCATTTCGTGGGTGTAGATTTAAGCACATTTGAAGGCTTAGAATAT
GCCGATATAGCTCAAAGTAAAGAGGGAGATTTATGTCCTAAGTGTGCCCAAGAGCTTTATTATACTAAAGGTATAGAAGT
TGGACATATTTTTAAATTAGGTGATAAATATTCGCGTGCAATGAATGCGCAATTTTTGGATAACGATGGCAAGACACAAC
CGCTTATTATGGGCTGCTATGGCTTTGGAATCTCGCGTATTTTGCCTGCGATATTAGAGCAAAAAAGCGATGATTTAGGC
TGTATATGGAGCAAAGAAGTGAGTGTTTTTGATATAGCCATTATTATTTCAAACACTAAAGATTCTGTGCAAAATGATTT
TGGTAGTGCTTTATATGAGATATTGAGTGCGTGTGGCATTGATGTTTTGTTAGATGAGCGAGATGAGCGATTTGGTGTGA
AGATGAAAGATTTTGAATTGCTTGGTTTTCATTCTGCTTTGATTGTAGGTAAGGGTTTAAATGAGGGCAAAGTAGAGTTG
ATTAAACGCGAAGGCTTAAAAAAATATGAGCTTTGTGCCACTGATAAAGAAATATTGCTTGAAGAGATTCTCAAAATCAT
AGCATAA

Upstream 100 bases:

>100_bases
TATTATTGAAGCAATGAAAAATGTGTTTGATATTAGTGATGATGTTGTTATGCTTAATGGCTATAAATGTGAGAAAGACA
CCATTTTTTAAAGGAATACT

Downstream 100 bases:

>100_bases
GGAGTGGATATGGCAAAGCAGAGCGGAGCAGATAGAGAGCTAGTTATTGGCTCACGTGGAAGTATATTGGCTTTATGGCA
AGCTGAATATATCAAGTCTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MRFSQLFVNTLKESPKDAVLKSHQYLIRGGFIQQIGSGIYNFLPLGKKLLDKVRFIVKEEMDKSGAQEILMGFVTPAELW
RESGRYEQYGRELLRFVDRKENEFVLGPTHEEVITHIAKNTIKSYKQLPLHLYQIHSKFRDELRPRFGLMRGREFIMKDG
YSFHSNYADLNREFDVMEATYKRILQRMGLEFKVVEADSGAIGGSGSKEFMVLAPCGEDTIVVCKGCEYGANIEASKRAP
RTAPRPNEIKYDSNAPQAAFARFFTPDIKNIESLSAFFKVDKFWTIKAIVKKAIKANNESELVYFFVRGDDEGEETKMLN
AINKHTNCYLALEDASVEEIQAAGLEVGFIGAYGLRHITQATHIYFDESLRDASNLICGANEKDYHFVGVDLSTFEGLEY
ADIAQSKEGDLCPKCAQELYYTKGIEVGHIFKLGDKYSRAMNAQFLDNDGKTQPLIMGCYGFGISRILPAILEQKSDDLG
CIWSKEVSVFDIAIIISNTKDSVQNDFGSALYEILSACGIDVLLDERDERFGVKMKDFELLGFHSALIVGKGLNEGKVEL
IKREGLKKYELCATDKEILLEEILKIIA

Sequences:

>Translated_588_residues
MRFSQLFVNTLKESPKDAVLKSHQYLIRGGFIQQIGSGIYNFLPLGKKLLDKVRFIVKEEMDKSGAQEILMGFVTPAELW
RESGRYEQYGRELLRFVDRKENEFVLGPTHEEVITHIAKNTIKSYKQLPLHLYQIHSKFRDELRPRFGLMRGREFIMKDG
YSFHSNYADLNREFDVMEATYKRILQRMGLEFKVVEADSGAIGGSGSKEFMVLAPCGEDTIVVCKGCEYGANIEASKRAP
RTAPRPNEIKYDSNAPQAAFARFFTPDIKNIESLSAFFKVDKFWTIKAIVKKAIKANNESELVYFFVRGDDEGEETKMLN
AINKHTNCYLALEDASVEEIQAAGLEVGFIGAYGLRHITQATHIYFDESLRDASNLICGANEKDYHFVGVDLSTFEGLEY
ADIAQSKEGDLCPKCAQELYYTKGIEVGHIFKLGDKYSRAMNAQFLDNDGKTQPLIMGCYGFGISRILPAILEQKSDDLG
CIWSKEVSVFDIAIIISNTKDSVQNDFGSALYEILSACGIDVLLDERDERFGVKMKDFELLGFHSALIVGKGLNEGKVEL
IKREGLKKYELCATDKEILLEEILKIIA
>Mature_588_residues
MRFSQLFVNTLKESPKDAVLKSHQYLIRGGFIQQIGSGIYNFLPLGKKLLDKVRFIVKEEMDKSGAQEILMGFVTPAELW
RESGRYEQYGRELLRFVDRKENEFVLGPTHEEVITHIAKNTIKSYKQLPLHLYQIHSKFRDELRPRFGLMRGREFIMKDG
YSFHSNYADLNREFDVMEATYKRILQRMGLEFKVVEADSGAIGGSGSKEFMVLAPCGEDTIVVCKGCEYGANIEASKRAP
RTAPRPNEIKYDSNAPQAAFARFFTPDIKNIESLSAFFKVDKFWTIKAIVKKAIKANNESELVYFFVRGDDEGEETKMLN
AINKHTNCYLALEDASVEEIQAAGLEVGFIGAYGLRHITQATHIYFDESLRDASNLICGANEKDYHFVGVDLSTFEGLEY
ADIAQSKEGDLCPKCAQELYYTKGIEVGHIFKLGDKYSRAMNAQFLDNDGKTQPLIMGCYGFGISRILPAILEQKSDDLG
CIWSKEVSVFDIAIIISNTKDSVQNDFGSALYEILSACGIDVLLDERDERFGVKMKDFELLGFHSALIVGKGLNEGKVEL
IKREGLKKYELCATDKEILLEEILKIIA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=229, Percent_Identity=41.4847161572052, Blast_Score=194, Evalue=3e-49,
Organism=Escherichia coli, GI1786392, Length=590, Percent_Identity=42.2033898305085, Blast_Score=445, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI115532348, Length=262, Percent_Identity=37.7862595419847, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI193203271, Length=106, Percent_Identity=36.7924528301887, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=552, Percent_Identity=29.3478260869565, Blast_Score=219, Evalue=8e-58,
Organism=Drosophila melanogaster, GI24656200, Length=254, Percent_Identity=36.2204724409449, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_HELHP (Q7VFF0)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_861257.1
- ProteinModelPortal:   Q7VFF0
- SMR:   Q7VFF0
- GeneID:   1492273
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1726
- NMPDR:   fig|235279.1.peg.1726
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   HHEP235279:HH_1726-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 66484; Mature: 66484

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSQLFVNTLKESPKDAVLKSHQYLIRGGFIQQIGSGIYNFLPLGKKLLDKVRFIVKEE
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MDKSGAQEILMGFVTPAELWRESGRYEQYGRELLRFVDRKENEFVLGPTHEEVITHIAKN
HCCCHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
TIKSYKQLPLHLYQIHSKFRDELRPRFGLMRGREFIMKDGYSFHSNYADLNREFDVMEAT
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHCCCCHHHHHHH
YKRILQRMGLEFKVVEADSGAIGGSGSKEFMVLAPCGEDTIVVCKGCEYGANIEASKRAP
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCC
RTAPRPNEIKYDSNAPQAAFARFFTPDIKNIESLSAFFKVDKFWTIKAIVKKAIKANNES
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ELVYFFVRGDDEGEETKMLNAINKHTNCYLALEDASVEEIQAAGLEVGFIGAYGLRHITQ
CEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCEEEEHHHHHHHHHH
ATHIYFDESLRDASNLICGANEKDYHFVGVDLSTFEGLEYADIAQSKEGDLCPKCAQELY
HHHEEECHHHHHHHHEEECCCCCCCEEEEEEHHHHCCCCHHHHHCCCCCCCCHHHHHHHH
YTKGIEVGHIFKLGDKYSRAMNAQFLDNDGKTQPLIMGCYGFGISRILPAILEQKSDDLG
HHCCCCCCCEEECCHHHHHHCCCCEECCCCCCCCEEEEHHHHCHHHHHHHHHHCCCCCCC
CIWSKEVSVFDIAIIISNTKDSVQNDFGSALYEILSACGIDVLLDERDERFGVKMKDFEL
EEEECCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHCCCEEECEEE
LGFHSALIVGKGLNEGKVELIKREGLKKYELCATDKEILLEEILKIIA
ECHHEEEEEECCCCCCHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MRFSQLFVNTLKESPKDAVLKSHQYLIRGGFIQQIGSGIYNFLPLGKKLLDKVRFIVKEE
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MDKSGAQEILMGFVTPAELWRESGRYEQYGRELLRFVDRKENEFVLGPTHEEVITHIAKN
HCCCHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
TIKSYKQLPLHLYQIHSKFRDELRPRFGLMRGREFIMKDGYSFHSNYADLNREFDVMEAT
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHCCCCHHHHHHH
YKRILQRMGLEFKVVEADSGAIGGSGSKEFMVLAPCGEDTIVVCKGCEYGANIEASKRAP
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCC
RTAPRPNEIKYDSNAPQAAFARFFTPDIKNIESLSAFFKVDKFWTIKAIVKKAIKANNES
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ELVYFFVRGDDEGEETKMLNAINKHTNCYLALEDASVEEIQAAGLEVGFIGAYGLRHITQ
CEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCEEEEHHHHHHHHHH
ATHIYFDESLRDASNLICGANEKDYHFVGVDLSTFEGLEYADIAQSKEGDLCPKCAQELY
HHHEEECHHHHHHHHEEECCCCCCCEEEEEEHHHHCCCCHHHHHCCCCCCCCHHHHHHHH
YTKGIEVGHIFKLGDKYSRAMNAQFLDNDGKTQPLIMGCYGFGISRILPAILEQKSDDLG
HHCCCCCCCEEECCHHHHHHCCCCEECCCCCCCCEEEEHHHHCHHHHHHHHHHCCCCCCC
CIWSKEVSVFDIAIIISNTKDSVQNDFGSALYEILSACGIDVLLDERDERFGVKMKDFEL
EEEECCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHCCCEEECEEE
LGFHSALIVGKGLNEGKVELIKREGLKKYELCATDKEILLEEILKIIA
ECHHEEEEEECCCCCCHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954