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The map label for this gene is hemC

Identifier: 32267226

GI number: 32267226

Start: 1653201

End: 1654166

Strand: Direct

Name: hemC

Synonym: HH1727

Alternate gene names: 32267226

Gene position: 1653201-1654166 (Clockwise)

Preceding gene: 32267225

Following gene: 32267228

Centisome position: 91.89

GC content: 38.72

Gene sequence:

>966_bases
ATGGCAAAGCAGAGCGGAGCAGATAGAGAGCTAGTTATTGGCTCACGTGGAAGTATATTGGCTTTATGGCAAGCTGAATA
TATCAAGTCTTGCCTTAAAGCTCAATGTGGTTTGCAATCTCGCATACAAATTATTAAAACTCGTGGTGATAAAATTCTTG
ATGTGCCTTTGGCAAAAATAGGTGGAAAGGGGCTTTTTACTAAAGAGTTAGAAGAAATGCTTTTAAGCAAAGATATTGAT
TTGGCTGTGCATAGCCTCAAAGATGTGCCTGTAGAATTTGTGCCTGAACTCGATTTAGCAGCTATTACTCAACGTGAGAG
TGCAAATGATTGTTTTTTGAGTGTAAATTATCCCAATCTTAATGCTTTACCACAAGGAGCAAAAGTAGGGACAACTTCCC
TACGCCGCTCAATGCAGATTAAAAAATATCGCTCTGATTTAGATACATTAAGTTTGCGTGGCAATGTGCAAACACGACTA
GAGAAACTCCATAATGGTGCATTTGATGCAATTATCCTCGCTCAAGCAGGAGTGAATAGGCTTAAAATCAATACACAAGA
TGTGCGTTATATCGTGCCCTTAGATTTTATGATACCTGCGATGGGGCAGGGTGCGCTTGGAATAGAAATGCGTAAGGATT
CTATATTTTTTGATAGAATTGCTGTGCTTAATGATAAGCAGAGTGCGCTTTGTGTGAGTGCAGAAAGGGCTTTTGTGAGG
ACATTAGAGGGTGGGTGTCAAGTGCCAATAGGCGTGTATGCACAATTTAGCAATAATAAACTCACACTTCAAGCTATAGT
AGGATTGCCTGATGGAAGTGAAGTATTACAAGATAGTGTCGAGGATTCTATAAATATAGATGATATAAATGCAAGCGAGA
ATCTAGGTATAGCATTTGCACAAAAATTTATTGATAAAGGTGCAAAAGAACTTCTTGAGCGAGCAGCAAAAATAGCATTT
GCATAA

Upstream 100 bases:

>100_bases
GTTGATTAAACGCGAAGGCTTAAAAAAATATGAGCTTTGTGCCACTGATAAAGAAATATTGCTTGAAGAGATTCTCAAAA
TCATAGCATAAGGAGTGGAT

Downstream 100 bases:

>100_bases
AAATTAAAACCAAAATCCCAAAAATAGTGTAATGTTACAATTTTAAATATAACTTAGCTTTTCTGTTCAAAATTTTTTGC
TTTTAAGCAAAAATCGGTGG

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MAKQSGADRELVIGSRGSILALWQAEYIKSCLKAQCGLQSRIQIIKTRGDKILDVPLAKIGGKGLFTKELEEMLLSKDID
LAVHSLKDVPVEFVPELDLAAITQRESANDCFLSVNYPNLNALPQGAKVGTTSLRRSMQIKKYRSDLDTLSLRGNVQTRL
EKLHNGAFDAIILAQAGVNRLKINTQDVRYIVPLDFMIPAMGQGALGIEMRKDSIFFDRIAVLNDKQSALCVSAERAFVR
TLEGGCQVPIGVYAQFSNNKLTLQAIVGLPDGSEVLQDSVEDSINIDDINASENLGIAFAQKFIDKGAKELLERAAKIAF
A

Sequences:

>Translated_321_residues
MAKQSGADRELVIGSRGSILALWQAEYIKSCLKAQCGLQSRIQIIKTRGDKILDVPLAKIGGKGLFTKELEEMLLSKDID
LAVHSLKDVPVEFVPELDLAAITQRESANDCFLSVNYPNLNALPQGAKVGTTSLRRSMQIKKYRSDLDTLSLRGNVQTRL
EKLHNGAFDAIILAQAGVNRLKINTQDVRYIVPLDFMIPAMGQGALGIEMRKDSIFFDRIAVLNDKQSALCVSAERAFVR
TLEGGCQVPIGVYAQFSNNKLTLQAIVGLPDGSEVLQDSVEDSINIDDINASENLGIAFAQKFIDKGAKELLERAAKIAF
A
>Mature_320_residues
AKQSGADRELVIGSRGSILALWQAEYIKSCLKAQCGLQSRIQIIKTRGDKILDVPLAKIGGKGLFTKELEEMLLSKDIDL
AVHSLKDVPVEFVPELDLAAITQRESANDCFLSVNYPNLNALPQGAKVGTTSLRRSMQIKKYRSDLDTLSLRGNVQTRLE
KLHNGAFDAIILAQAGVNRLKINTQDVRYIVPLDFMIPAMGQGALGIEMRKDSIFFDRIAVLNDKQSALCVSAERAFVRT
LEGGCQVPIGVYAQFSNNKLTLQAIVGLPDGSEVLQDSVEDSINIDDINASENLGIAFAQKFIDKGAKELLERAAKIAFA

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI66933009, Length=342, Percent_Identity=39.4736842105263, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI20149500, Length=342, Percent_Identity=39.4736842105263, Blast_Score=209, Evalue=2e-54,
Organism=Escherichia coli, GI48994974, Length=307, Percent_Identity=49.8371335504886, Blast_Score=276, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6319996, Length=320, Percent_Identity=39.375, Blast_Score=186, Evalue=4e-48,
Organism=Drosophila melanogaster, GI20130425, Length=267, Percent_Identity=36.3295880149813, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_HELHP (Q7VFE9)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_861258.1
- ProteinModelPortal:   Q7VFE9
- GeneID:   1492274
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1727
- NMPDR:   fig|235279.1.peg.1727
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BioCyc:   HHEP235279:HH_1727-MONOMER
- BRENDA:   2.5.1.61
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 35108; Mature: 34977

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQSGADRELVIGSRGSILALWQAEYIKSCLKAQCGLQSRIQIIKTRGDKILDVPLAKI
CCCCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCHHHH
GGKGLFTKELEEMLLSKDIDLAVHSLKDVPVEFVPELDLAAITQRESANDCFLSVNYPNL
CCCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHCCCCCHHHHHCCCCCCCEEEEECCCCC
NALPQGAKVGTTSLRRSMQIKKYRSDLDTLSLRGNVQTRLEKLHNGAFDAIILAQAGVNR
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHCCCHHEEEEECCCCCE
LKINTQDVRYIVPLDFMIPAMGQGALGIEMRKDSIFFDRIAVLNDKQSALCVSAERAFVR
EEEECCCEEEEEEHHHHHCCCCCCCEEEEEECCCCHHHHHEEECCCCCEEEEHHHHHHHH
TLEGGCQVPIGVYAQFSNNKLTLQAIVGLPDGSEVLQDSVEDSINIDDINASENLGIAFA
HHCCCCCCCEEEEEEECCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHH
QKFIDKGAKELLERAAKIAFA
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKQSGADRELVIGSRGSILALWQAEYIKSCLKAQCGLQSRIQIIKTRGDKILDVPLAKI
CCCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCHHHH
GGKGLFTKELEEMLLSKDIDLAVHSLKDVPVEFVPELDLAAITQRESANDCFLSVNYPNL
CCCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHCCCCCHHHHHCCCCCCCEEEEECCCCC
NALPQGAKVGTTSLRRSMQIKKYRSDLDTLSLRGNVQTRLEKLHNGAFDAIILAQAGVNR
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHCCCHHEEEEECCCCCE
LKINTQDVRYIVPLDFMIPAMGQGALGIEMRKDSIFFDRIAVLNDKQSALCVSAERAFVR
EEEECCCEEEEEEHHHHHCCCCCCCEEEEEECCCCHHHHHEEECCCCCEEEEHHHHHHHH
TLEGGCQVPIGVYAQFSNNKLTLQAIVGLPDGSEVLQDSVEDSINIDDINASENLGIAFA
HHCCCCCCCEEEEEEECCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHH
QKFIDKGAKELLERAAKIAFA
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954