Click here to switch to the map view.

The map label for this gene is hemA

Identifier: 32267224

GI number: 32267224

Start: 1650093

End: 1651415

Strand: Direct

Name: hemA

Synonym: HH1725

Alternate gene names: 32267224

Gene position: 1650093-1651415 (Clockwise)

Preceding gene: 32267223

Following gene: 32267225

Centisome position: 91.72

GC content: 36.13

Gene sequence:

>1323_bases
ATGCAAGAAAAAATGGAAGTGCAATATATGGTGGTAAGTTTTTCACACAAAAATGTAGATATTGCTACGCGTGAGAAACT
GAGTTTTAGCCAAGAAGAGATTGTGCCATTTTTGCAAGAGATTAATGTATGCGATAGTATTAGGGAGAGTATTTTACTTT
GCACTTGTAATCGTGTGGAGCTTTATGTAAGTATGATTGATAAAAAAAGAGCAAGAGAACATATCTATGAATGTTTTTGC
ACACATAAGAATATAGCATTAGAGGATATAAAAAATATTGCACTTATGCGTTTAAACCAATATGCGATTTATCATATTTT
TAGTGTGGCTTCAAGCCTTGATAGTTTGGTTATAGGTGAGACACAGATTACAGGACAACTTAAGCTTGCTTATAAACTTG
CCTTTGAGAATGCGCTTTGTGCCAAAGATATGACTCGTCTTATGCACTTTGCCTTTAAGTGTGCTGCAAGCGTGCGAAAA
GAGACAGATATTTCAGCTCATAGTGTATCGGTAGCTTCTACGGCAGTGCGTATGGCAGAACAAAAACTTGCTTTGTGTGA
TAAAACTTTAGAGAATCTGCCTGTTTTAGTTATCGGCAGTGGCGAAATGGGGCGTCTTGCGTGTAAGCATCTCCAAAATG
CTAACGCTCAAATTACACTTGTTAGTCGCACAAAAGAAAATGCACGCAAACTTGCCTTAGAACTTGACTCTAGTATAAAT
ATAGAATCTTGGGAGCATTTAGAGCAACTTTTAGGGCAATATGAAGTGCTTTTTAGCGCTACGAGTGCTCCAAATTGTAT
TATTCAATCAAAGATGGTGCAAGTGAGCCAAAAGCAAAGATGGTGGTTTGATTTGGCTTTGCCGCGTGATATAGAAAATA
TACAAATGGAAAATTTACATATTTTTTGTGTTGATGATTTGGAAGAAATTGTGCAAGAGCATAAAAATGCGCGTGAAGAT
AGTGCAAAAAAAGCACAAAAGATTCTTGAACGTTATAGTGTAGAATTTTTTAAATGGCTTCAAACTTTGGGGATTGACCC
TATCATTAAACATATTCGTTATCTTGCCAAACAATCTGCCCTCAAAGAGCTTGATAGAGCGGTAAAAAAAGGATTTTTAC
CTGCTTCGTATCAACAAAATGTAGAGAAGATTTTGCACGGAGCTTTTAATACCTTTTTGCATCAACCAACAATACGGCTC
AAGCAAGCAAGTGAGAATCCTCAAGGCGACCCTATTATTGAAGCAATGAAAAATGTGTTTGATATTAGTGATGATGTTGT
TATGCTTAATGGCTATAAATGTGAGAAAGACACCATTTTTTAA

Upstream 100 bases:

>100_bases
TATGCTAATAAAGCCATTCAATCTATTGTAAATGAGGGCAATGAGAAGCTTAAAGATATTGTTACACAAATGATTGAGAG
GACATTTTAGGGAGGATAGA

Downstream 100 bases:

>100_bases
AGGAATACTATGAGGTTTTCACAACTTTTTGTAAATACTCTTAAAGAATCTCCTAAAGATGCAGTGCTTAAAAGTCATCA
ATATCTTATTCGTGGGGGCT

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MQEKMEVQYMVVSFSHKNVDIATREKLSFSQEEIVPFLQEINVCDSIRESILLCTCNRVELYVSMIDKKRAREHIYECFC
THKNIALEDIKNIALMRLNQYAIYHIFSVASSLDSLVIGETQITGQLKLAYKLAFENALCAKDMTRLMHFAFKCAASVRK
ETDISAHSVSVASTAVRMAEQKLALCDKTLENLPVLVIGSGEMGRLACKHLQNANAQITLVSRTKENARKLALELDSSIN
IESWEHLEQLLGQYEVLFSATSAPNCIIQSKMVQVSQKQRWWFDLALPRDIENIQMENLHIFCVDDLEEIVQEHKNARED
SAKKAQKILERYSVEFFKWLQTLGIDPIIKHIRYLAKQSALKELDRAVKKGFLPASYQQNVEKILHGAFNTFLHQPTIRL
KQASENPQGDPIIEAMKNVFDISDDVVMLNGYKCEKDTIF

Sequences:

>Translated_440_residues
MQEKMEVQYMVVSFSHKNVDIATREKLSFSQEEIVPFLQEINVCDSIRESILLCTCNRVELYVSMIDKKRAREHIYECFC
THKNIALEDIKNIALMRLNQYAIYHIFSVASSLDSLVIGETQITGQLKLAYKLAFENALCAKDMTRLMHFAFKCAASVRK
ETDISAHSVSVASTAVRMAEQKLALCDKTLENLPVLVIGSGEMGRLACKHLQNANAQITLVSRTKENARKLALELDSSIN
IESWEHLEQLLGQYEVLFSATSAPNCIIQSKMVQVSQKQRWWFDLALPRDIENIQMENLHIFCVDDLEEIVQEHKNARED
SAKKAQKILERYSVEFFKWLQTLGIDPIIKHIRYLAKQSALKELDRAVKKGFLPASYQQNVEKILHGAFNTFLHQPTIRL
KQASENPQGDPIIEAMKNVFDISDDVVMLNGYKCEKDTIF
>Mature_440_residues
MQEKMEVQYMVVSFSHKNVDIATREKLSFSQEEIVPFLQEINVCDSIRESILLCTCNRVELYVSMIDKKRAREHIYECFC
THKNIALEDIKNIALMRLNQYAIYHIFSVASSLDSLVIGETQITGQLKLAYKLAFENALCAKDMTRLMHFAFKCAASVRK
ETDISAHSVSVASTAVRMAEQKLALCDKTLENLPVLVIGSGEMGRLACKHLQNANAQITLVSRTKENARKLALELDSSIN
IESWEHLEQLLGQYEVLFSATSAPNCIIQSKMVQVSQKQRWWFDLALPRDIENIQMENLHIFCVDDLEEIVQEHKNARED
SAKKAQKILERYSVEFFKWLQTLGIDPIIKHIRYLAKQSALKELDRAVKKGFLPASYQQNVEKILHGAFNTFLHQPTIRL
KQASENPQGDPIIEAMKNVFDISDDVVMLNGYKCEKDTIF

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=409, Percent_Identity=29.5843520782396, Blast_Score=172, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_HELHP (Q7VFF1)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_861256.1
- ProteinModelPortal:   Q7VFF1
- SMR:   Q7VFF1
- GeneID:   1492272
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1725
- NMPDR:   fig|235279.1.peg.1725
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- ProtClustDB:   PRK00045
- BioCyc:   HHEP235279:HH_1725-MONOMER
- BRENDA:   1.2.1.70
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 50371; Mature: 50371

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 56-56 BINDING 115-115 BINDING 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEKMEVQYMVVSFSHKNVDIATREKLSFSQEEIVPFLQEINVCDSIRESILLCTCNRVE
CCCCCEEEEEEEEECCCCCCEEHHHHHCCCHHHHHHHHHHCCHHHHHHHHHEEEECCHHH
LYVSMIDKKRAREHIYECFCTHKNIALEDIKNIALMRLNQYAIYHIFSVASSLDSLVIGE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
TQITGQLKLAYKLAFENALCAKDMTRLMHFAFKCAASVRKETDISAHSVSVASTAVRMAE
EEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QKLALCDKTLENLPVLVIGSGEMGRLACKHLQNANAQITLVSRTKENARKLALELDSSIN
HHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCC
IESWEHLEQLLGQYEVLFSATSAPNCIIQSKMVQVSQKQRWWFDLALPRDIENIQMENLH
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCEECCEE
IFCVDDLEEIVQEHKNAREDSAKKAQKILERYSVEFFKWLQTLGIDPIIKHIRYLAKQSA
EEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LKELDRAVKKGFLPASYQQNVEKILHGAFNTFLHQPTIRLKQASENPQGDPIIEAMKNVF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHH
DISDDVVMLNGYKCEKDTIF
CCCCCEEEEECEECCCCCCC
>Mature Secondary Structure
MQEKMEVQYMVVSFSHKNVDIATREKLSFSQEEIVPFLQEINVCDSIRESILLCTCNRVE
CCCCCEEEEEEEEECCCCCCEEHHHHHCCCHHHHHHHHHHCCHHHHHHHHHEEEECCHHH
LYVSMIDKKRAREHIYECFCTHKNIALEDIKNIALMRLNQYAIYHIFSVASSLDSLVIGE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
TQITGQLKLAYKLAFENALCAKDMTRLMHFAFKCAASVRKETDISAHSVSVASTAVRMAE
EEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QKLALCDKTLENLPVLVIGSGEMGRLACKHLQNANAQITLVSRTKENARKLALELDSSIN
HHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCC
IESWEHLEQLLGQYEVLFSATSAPNCIIQSKMVQVSQKQRWWFDLALPRDIENIQMENLH
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCEECCEE
IFCVDDLEEIVQEHKNAREDSAKKAQKILERYSVEFFKWLQTLGIDPIIKHIRYLAKQSA
EEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LKELDRAVKKGFLPASYQQNVEKILHGAFNTFLHQPTIRLKQASENPQGDPIIEAMKNVF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHH
DISDDVVMLNGYKCEKDTIF
CCCCCEEEEECEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954