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The map label for this gene is cdtB

Identifier: 32266946

GI number: 32266946

Start: 1395895

End: 1396716

Strand: Direct

Name: cdtB

Synonym: HH1447

Alternate gene names: 32266946

Gene position: 1395895-1396716 (Clockwise)

Preceding gene: 32266945

Following gene: 32266947

Centisome position: 77.59

GC content: 40.75

Gene sequence:

>822_bases
ATGAGAATACTATTATGCTTTTTAATGAGTTTTACCTTTGCTTTAGCCAATCTTGAAGATTATAGAGTAAGCACTTGGAA
TCTACAAGGTTCTTCAGCAAATACTGAGAGTAAATGGAATATAAGTGTGAGACAACTCATCACAGGAGATAATCCTGCTA
ATATTCTTATGGTGCAAGAGGCTGGCGCTATACCTGCTTCAGCGAGAAGAACAGGTAGAATGGTTCAGCCCGGTGGTACA
CCTGTAGAGGAGTTTACGTGGGAACTTGGAACTTATTCTAGACCAAATACAGTTTATATCTATTATGCTCCTCTAGATGT
GGGAGCAAGAAGGGTGAATCTCGCCATAGTTTCTGATAGAAGAGCTGATGAAGTTCTTGTAGTGCATCAAAATGTTGTTG
CTACAGAGGCATCGCGTCCAGCTATTGGTATTCGTATCGGCAATGATGTGTTTTTTAATATTCACGCTTTAGCAAGTGGA
GGTGGTGATGCACCTGCTTTAGTTACAGCTGTGCACGATAATTTTATTAATATGCCACAAATAAATTGGCTTATCGCTGG
AGATTTTAATCGCGATCCAGCGCTCTTGCAATCAGGGCTAGATACAAGAATCGCTAATCATATCCGTATTACTGCTCCAA
ACTCTGCTACACATTTTAGCTCTAGAGGAACAAATAGAACACTTGATTATGCGGTAGTAGGTAGATCAAGCCCTTCTCGT
TCCACTATAGTATTGCCGCAAATTGCAGCAATACTTATGGCAGCAAATATACGCGCACACCTCTCATCTGACCATTCACC
TGTGCATTTTGGACGATTTTAG

Upstream 100 bases:

>100_bases
GCGACGAGTTCTAACCTTGACCAACAATGGTATATTACCCCTGTGGCACTAGAACCAGAGCCTATTTTTTTCATTAAATA
ATCAATATTAAGGAGTAAAA

Downstream 100 bases:

>100_bases
GAGGCATCAATGAAAAAATTTACTTTATTATTCATTTTAATCTCAAGCTTTGTATATGGAGGTGGTCCTGAAGATTTACC
AGATTTTACTGCACCATTTT

Product: cytolethal distending toxin CdtB

Products: NA

Alternate protein names: CDT B; Deoxyribonuclease CdtB [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MRILLCFLMSFTFALANLEDYRVSTWNLQGSSANTESKWNISVRQLITGDNPANILMVQEAGAIPASARRTGRMVQPGGT
PVEEFTWELGTYSRPNTVYIYYAPLDVGARRVNLAIVSDRRADEVLVVHQNVVATEASRPAIGIRIGNDVFFNIHALASG
GGDAPALVTAVHDNFINMPQINWLIAGDFNRDPALLQSGLDTRIANHIRITAPNSATHFSSRGTNRTLDYAVVGRSSPSR
STIVLPQIAAILMAANIRAHLSSDHSPVHFGRF

Sequences:

>Translated_273_residues
MRILLCFLMSFTFALANLEDYRVSTWNLQGSSANTESKWNISVRQLITGDNPANILMVQEAGAIPASARRTGRMVQPGGT
PVEEFTWELGTYSRPNTVYIYYAPLDVGARRVNLAIVSDRRADEVLVVHQNVVATEASRPAIGIRIGNDVFFNIHALASG
GGDAPALVTAVHDNFINMPQINWLIAGDFNRDPALLQSGLDTRIANHIRITAPNSATHFSSRGTNRTLDYAVVGRSSPSR
STIVLPQIAAILMAANIRAHLSSDHSPVHFGRF
>Mature_273_residues
MRILLCFLMSFTFALANLEDYRVSTWNLQGSSANTESKWNISVRQLITGDNPANILMVQEAGAIPASARRTGRMVQPGGT
PVEEFTWELGTYSRPNTVYIYYAPLDVGARRVNLAIVSDRRADEVLVVHQNVVATEASRPAIGIRIGNDVFFNIHALASG
GGDAPALVTAVHDNFINMPQINWLIAGDFNRDPALLQSGLDTRIANHIRITAPNSATHFSSRGTNRTLDYAVVGRSSPSR
STIVLPQIAAILMAANIRAHLSSDHSPVHFGRF

Specific function: Part of the tripartite complex that is required for the CDT activity. CdtB exhibits a DNA-nicking endonuclease activity, and very probably causes DNA damage in intoxicated cells. This damage induces G2/M cell cycle arrest, chromatin fragmentation, cell di

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Localized to the nucleus of the infected cells [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003539
- InterPro:   IPR005135 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: NA

Molecular weight: Translated: 29807; Mature: 29807

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILLCFLMSFTFALANLEDYRVSTWNLQGSSANTESKWNISVRQLITGDNPANILMVQE
CHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEEEEEEEECCCCCCEEEEEEC
AGAIPASARRTGRMVQPGGTPVEEFTWELGTYSRPNTVYIYYAPLDVGARRVNLAIVSDR
CCCCCCCHHHCCCEECCCCCCHHHHEECCCCCCCCCEEEEEEEECCCCCEEEEEEEEECC
RADEVLVVHQNVVATEASRPAIGIRIGNDVFFNIHALASGGGDAPALVTAVHDNFINMPQ
CCCEEEEEECCEEEECCCCCEEEEEECCEEEEEEEEEECCCCCCCEEEEEEECCCCCCCC
INWLIAGDFNRDPALLQSGLDTRIANHIRITAPNSATHFSSRGTNRTLDYAVVGRSSPSR
EEEEEEECCCCCHHHHHCCCCHHCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCC
STIVLPQIAAILMAANIRAHLSSDHSPVHFGRF
CEEEHHHHHHHHHHHHHHEECCCCCCCEECCCC
>Mature Secondary Structure
MRILLCFLMSFTFALANLEDYRVSTWNLQGSSANTESKWNISVRQLITGDNPANILMVQE
CHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEEEEEEEECCCCCCEEEEEEC
AGAIPASARRTGRMVQPGGTPVEEFTWELGTYSRPNTVYIYYAPLDVGARRVNLAIVSDR
CCCCCCCHHHCCCEECCCCCCHHHHEECCCCCCCCCEEEEEEEECCCCCEEEEEEEEECC
RADEVLVVHQNVVATEASRPAIGIRIGNDVFFNIHALASGGGDAPALVTAVHDNFINMPQ
CCCEEEEEECCEEEECCCCCEEEEEECCEEEEEEEEEECCCCCCCEEEEEEECCCCCCCC
INWLIAGDFNRDPALLQSGLDTRIANHIRITAPNSATHFSSRGTNRTLDYAVVGRSSPSR
EEEEEEECCCCCHHHHHCCCCHHCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCC
STIVLPQIAAILMAANIRAHLSSDHSPVHFGRF
CEEEHHHHHHHHHHHHHHEECCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8112838 [H]