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The map label for this gene is cdtA

Identifier: 32266945

GI number: 32266945

Start: 1395180

End: 1395875

Strand: Direct

Name: cdtA

Synonym: HH1446

Alternate gene names: 32266945

Gene position: 1395180-1395875 (Clockwise)

Preceding gene: 32266944

Following gene: 32266946

Centisome position: 77.55

GC content: 35.92

Gene sequence:

>696_bases
ATGAGACTTTTATTTTTTCTCTTAATAACTCTTTTGTTTGCAGCGTGTTCTTCAACACCAAAGGTTCATCAGCCTAAGCA
TTCGTCTAAGACTGAAAAAGATTTGGGTATAGGATTAAGTCCAACGCCTCCACCTAATGAACGAATACCAGGTGAAAAAT
CAAGAGCAATGTTGATGGAAATGGAAGGGCGTGAAATTCCTGTTAGGATTCCTGATGCAAATGCATCAAAAATAGATAAA
AATGTTTCTAATCCTATATCAATTATGTCTTCATCGGGAGGTCTTTTAACACTTTGGGCGCTTAAGCCAAGAAATTGGGT
ATGGGGATATACGCCTATTGATAGTTTTGAGTTTGGCAGAGCAAAATTTTGGCGTATTATAAGTTTTTCAAATGGGCAAG
TGATAATTAAAAATATGCAAGAAGGTACTTGTCTTCAGGCTTACGGAAATGGTGTGATTCACGATATATGCGATAGTAAA
AATCAAGCACAATTATGGAATCTCAACTTTTTTGACAATCAAGCCATACAAATTCAAAGTGTGAGTGCAAAAACTTGTTT
GCAAACACCAACTGTGCGAACAACGACATACTATAGTATTTATTTAACAAAATGTGCGACGAGTTCTAACCTTGACCAAC
AATGGTATATTACCCCTGTGGCACTAGAACCAGAGCCTATTTTTTTCATTAAATAA

Upstream 100 bases:

>100_bases
TCTTATTTACATTAATGTTTAAAATGTTTAAGTATTTAACTTTTGGTTATAGACTGCTATTGAGTGTATTTAATCAATTT
ATTTTACAAAGGATATAACA

Downstream 100 bases:

>100_bases
TCAATATTAAGGAGTAAAAATGAGAATACTATTATGCTTTTTAATGAGTTTTACCTTTGCTTTAGCCAATCTTGAAGATT
ATAGAGTAAGCACTTGGAAT

Product: cytolethal distending toxin CdtA

Products: NA

Alternate protein names: CDT A

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MRLLFFLLITLLFAACSSTPKVHQPKHSSKTEKDLGIGLSPTPPPNERIPGEKSRAMLMEMEGREIPVRIPDANASKIDK
NVSNPISIMSSSGGLLTLWALKPRNWVWGYTPIDSFEFGRAKFWRIISFSNGQVIIKNMQEGTCLQAYGNGVIHDICDSK
NQAQLWNLNFFDNQAIQIQSVSAKTCLQTPTVRTTTYYSIYLTKCATSSNLDQQWYITPVALEPEPIFFIK

Sequences:

>Translated_231_residues
MRLLFFLLITLLFAACSSTPKVHQPKHSSKTEKDLGIGLSPTPPPNERIPGEKSRAMLMEMEGREIPVRIPDANASKIDK
NVSNPISIMSSSGGLLTLWALKPRNWVWGYTPIDSFEFGRAKFWRIISFSNGQVIIKNMQEGTCLQAYGNGVIHDICDSK
NQAQLWNLNFFDNQAIQIQSVSAKTCLQTPTVRTTTYYSIYLTKCATSSNLDQQWYITPVALEPEPIFFIK
>Mature_231_residues
MRLLFFLLITLLFAACSSTPKVHQPKHSSKTEKDLGIGLSPTPPPNERIPGEKSRAMLMEMEGREIPVRIPDANASKIDK
NVSNPISIMSSSGGLLTLWALKPRNWVWGYTPIDSFEFGRAKFWRIISFSNGQVIIKNMQEGTCLQAYGNGVIHDICDSK
NQAQLWNLNFFDNQAIQIQSVSAKTCLQTPTVRTTTYYSIYLTKCATSSNLDQQWYITPVALEPEPIFFIK

Specific function: CDTs are cytotoxins which induce cell distension, growth arrest in G2/M phase, nucleus swelling, and chromatin fragmentation in HeLa cells

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ricin B-type lectin domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CDTA_HELHP (Q9RFY6)

Other databases:

- EMBL:   AF163667
- EMBL:   AE017125
- RefSeq:   NP_860977.1
- ProteinModelPortal:   Q9RFY6
- SMR:   Q9RFY6
- GeneID:   1493134
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1446
- NMPDR:   fig|235279.1.peg.1446
- HOGENOM:   HBG395212
- OMA:   MEMEGRE
- BioCyc:   HHEP235279:HH_1446-MONOMER
- GO:   GO:0009405
- InterPro:   IPR015957
- InterPro:   IPR003558
- InterPro:   IPR008997
- InterPro:   IPR000772
- PIRSF:   PIRSF036516
- PRINTS:   PR01387

Pfam domain/function: PF03498 CDtoxinA; SSF50370 RicinB_like

EC number: NA

Molecular weight: Translated: 26021; Mature: 26021

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS50231 RICIN_B_LECTIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLFFLLITLLFAACSSTPKVHQPKHSSKTEKDLGIGLSPTPPPNERIPGEKSRAMLME
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEE
MEGREIPVRIPDANASKIDKNVSNPISIMSSSGGLLTLWALKPRNWVWGYTPIDSFEFGR
ECCCEEEEECCCCCHHHHHCCCCCCEEEEECCCCEEEEEEECCCCEEEECCCCCCCCCCC
AKFWRIISFSNGQVIIKNMQEGTCLQAYGNGVIHDICDSKNQAQLWNLNFFDNQAIQIQS
EEEEEEEEECCCEEEEEECCCCCEEEECCCCEEEHHHCCCCCCEEEEEEECCCCEEEEEE
VSAKTCLQTPTVRTTTYYSIYLTKCATSSNLDQQWYITPVALEPEPIFFIK
CCHHHHHCCCCEEEEEEEEEEEEEECCCCCCCCEEEEEEEEECCCCEEEEC
>Mature Secondary Structure
MRLLFFLLITLLFAACSSTPKVHQPKHSSKTEKDLGIGLSPTPPPNERIPGEKSRAMLME
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEE
MEGREIPVRIPDANASKIDKNVSNPISIMSSSGGLLTLWALKPRNWVWGYTPIDSFEFGR
ECCCEEEEECCCCCHHHHHCCCCCCEEEEECCCCEEEEEEECCCCEEEECCCCCCCCCCC
AKFWRIISFSNGQVIIKNMQEGTCLQAYGNGVIHDICDSKNQAQLWNLNFFDNQAIQIQS
EEEEEEEEECCCEEEEEECCCCCEEEECCCCEEEHHHCCCCCCEEEEEEECCCCEEEEEE
VSAKTCLQTPTVRTTTYYSIYLTKCATSSNLDQQWYITPVALEPEPIFFIK
CCHHHHHCCCCEEEEEEEEEEEEEECCCCCCCCEEEEEEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10603386; 12810954