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The map label for this gene is pyrG

Identifier: 32266849

GI number: 32266849

Start: 1305554

End: 1307176

Strand: Direct

Name: pyrG

Synonym: HH1350

Alternate gene names: 32266849

Gene position: 1305554-1307176 (Clockwise)

Preceding gene: 32266846

Following gene: 32266850

Centisome position: 72.57

GC content: 37.65

Gene sequence:

>1623_bases
ATGGCAGTAAAATATATTTTTGTAACAGGAGGTGTCTTAAGTTCCCTTGGTAAGGGTATTACTTCTTCTTCCATCGCAAC
TTTACTTCAACAGAGCGGATATAGTGTATCAATTTTAAAAATTGACCCTTATATTAATGTTGATCCGGGCACGATGAGTC
CATTAGAACACGGAGAAGTTTTTGTAACCTGTGATGGAGCAGAAACAGATTTGGATATAGGGCATTATGAGCGGTTTTTG
AATAAAGATTTCCATAAAAAAAATAATTTTACCACAGGACAAATTTATATGTCTGTGATTGAAAATGAGCGCAAGGGTAA
ATATTTAGGCAAAACCATTCAGATTGTGCCGCATATTGTTGATGAAATTAAATCTCGTATCAAGTTTGCAGGAGAGGGAA
GTGATTTTCTTGTTGTAGAACTTGGTGGAACAGTTGGTGATATAGAGGGTATGCCTTATCTTGAAGCTATGCGACAGATG
AAGCACGAGCTAGGAGGCAAACAAGTTATTTCTATTCATATTACGCTTATTCCTCTTATGCGTGCAGCAGGAGAGCTAAA
GACAAAGCCTACCCAACATTCCGTTCAAGAACTTAGACGCATTGGTATTTCTCCACAAATTCTTGTAGCGCGATGTGAGA
AAAATCTTGACAAAGAGTTAAAGCGCAAACTTGCTTTGAGTTGTGATGTTGATAATGATAGTGTGATAGTGGCTCAAGAT
ACGCAGAGTATTTATAAATGTCCTCTTAATTTCTTAGAAGAAGGCATACTTACTCCTATTGCAAGGCATTTGGAGTTGGG
CGAATTAAAACCCAAAATGGATAATTGGGATATGCTTGTAAAAAAAATCATTGCACCCAAATCAAACATAACCATTGGTT
TTGTCGGCAAATATTTAAGCCTCAAAGAATCTTACAAGTCTTTAATAGAATCTCTTATACACGCAGGAGCAAACACCGAC
ACACGTGTAAATATCAAATGGATTGATAGTGAGATTTTAGTAGATAATCTTGCGCTTTTATATGATGTAGATTCTCTTCT
TATACCGGGAGGATTTGGCGAGCGGGGCATTGAAGGTAAATTAGAGGCAATAAAATATGCGCGTATCCAGCAGATTCCAT
TACTTGGTATTTGTTTAGGTATGCAGCTCTCACTCATTGAATTTGCGCGCAATGTATTAGGAATGAGCGAAGCAAACTCT
ATTGAATTTGATCCACAAACAAAAGAGCCTGTGATATATTTAATTGAAAATTTTATCGATTCGCAAGGTGGGGTGCAATT
ACGCACGCATACTTCACCTATGGGTGGAACAATGCGTTTGGGTGAGTATGAATGCCATATCAAAAAAGGCACAAAGCTTT
ATGAGGCTTATGGCAAACAGACACTTATTAAAGAGCGTCATCGTCATAGATACGAGGCAAATCCTCACTATCGCGCACTT
TTTGAAAAAAATGGTATGATTGTAAGTGGAGAGAGCAATGGACTTATAGAATCTATTGAGCTTGCAAATCACCCGTGGTT
TGTTGGAGTGCAGTTTCACCCTGAATTTACTTCGCGATTGCAAAACCCTAATCCTGTCATTTTGGCTTTTGTGAAAGAAA
CTTTAGCACATAAAAAGACATAA

Upstream 100 bases:

>100_bases
AAAACATATTATGGAATATAAAATACAAAAAAAAGTAAATTAACAAGCTTTTAACTACTCATTTATTATGATTACATCAA
AATTTATGAAGGATTAGCTA

Downstream 100 bases:

>100_bases
ATAAATTGGTATAAAAATGCGTCAGATTGATAAAGATACCCTTTTACAGATGCTCTTAGAGCGTGATTTAGAGCAAGGTA
TAGCGACCTTACGTGATTTG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MAVKYIFVTGGVLSSLGKGITSSSIATLLQQSGYSVSILKIDPYINVDPGTMSPLEHGEVFVTCDGAETDLDIGHYERFL
NKDFHKKNNFTTGQIYMSVIENERKGKYLGKTIQIVPHIVDEIKSRIKFAGEGSDFLVVELGGTVGDIEGMPYLEAMRQM
KHELGGKQVISIHITLIPLMRAAGELKTKPTQHSVQELRRIGISPQILVARCEKNLDKELKRKLALSCDVDNDSVIVAQD
TQSIYKCPLNFLEEGILTPIARHLELGELKPKMDNWDMLVKKIIAPKSNITIGFVGKYLSLKESYKSLIESLIHAGANTD
TRVNIKWIDSEILVDNLALLYDVDSLLIPGGFGERGIEGKLEAIKYARIQQIPLLGICLGMQLSLIEFARNVLGMSEANS
IEFDPQTKEPVIYLIENFIDSQGGVQLRTHTSPMGGTMRLGEYECHIKKGTKLYEAYGKQTLIKERHRHRYEANPHYRAL
FEKNGMIVSGESNGLIESIELANHPWFVGVQFHPEFTSRLQNPNPVILAFVKETLAHKKT

Sequences:

>Translated_540_residues
MAVKYIFVTGGVLSSLGKGITSSSIATLLQQSGYSVSILKIDPYINVDPGTMSPLEHGEVFVTCDGAETDLDIGHYERFL
NKDFHKKNNFTTGQIYMSVIENERKGKYLGKTIQIVPHIVDEIKSRIKFAGEGSDFLVVELGGTVGDIEGMPYLEAMRQM
KHELGGKQVISIHITLIPLMRAAGELKTKPTQHSVQELRRIGISPQILVARCEKNLDKELKRKLALSCDVDNDSVIVAQD
TQSIYKCPLNFLEEGILTPIARHLELGELKPKMDNWDMLVKKIIAPKSNITIGFVGKYLSLKESYKSLIESLIHAGANTD
TRVNIKWIDSEILVDNLALLYDVDSLLIPGGFGERGIEGKLEAIKYARIQQIPLLGICLGMQLSLIEFARNVLGMSEANS
IEFDPQTKEPVIYLIENFIDSQGGVQLRTHTSPMGGTMRLGEYECHIKKGTKLYEAYGKQTLIKERHRHRYEANPHYRAL
FEKNGMIVSGESNGLIESIELANHPWFVGVQFHPEFTSRLQNPNPVILAFVKETLAHKKT
>Mature_539_residues
AVKYIFVTGGVLSSLGKGITSSSIATLLQQSGYSVSILKIDPYINVDPGTMSPLEHGEVFVTCDGAETDLDIGHYERFLN
KDFHKKNNFTTGQIYMSVIENERKGKYLGKTIQIVPHIVDEIKSRIKFAGEGSDFLVVELGGTVGDIEGMPYLEAMRQMK
HELGGKQVISIHITLIPLMRAAGELKTKPTQHSVQELRRIGISPQILVARCEKNLDKELKRKLALSCDVDNDSVIVAQDT
QSIYKCPLNFLEEGILTPIARHLELGELKPKMDNWDMLVKKIIAPKSNITIGFVGKYLSLKESYKSLIESLIHAGANTDT
RVNIKWIDSEILVDNLALLYDVDSLLIPGGFGERGIEGKLEAIKYARIQQIPLLGICLGMQLSLIEFARNVLGMSEANSI
EFDPQTKEPVIYLIENFIDSQGGVQLRTHTSPMGGTMRLGEYECHIKKGTKLYEAYGKQTLIKERHRHRYEANPHYRALF
EKNGMIVSGESNGLIESIELANHPWFVGVQFHPEFTSRLQNPNPVILAFVKETLAHKKT

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=550, Percent_Identity=44, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI28559085, Length=556, Percent_Identity=42.4460431654676, Blast_Score=444, Evalue=1e-125,
Organism=Homo sapiens, GI28559083, Length=556, Percent_Identity=42.4460431654676, Blast_Score=444, Evalue=1e-125,
Organism=Homo sapiens, GI221316689, Length=556, Percent_Identity=42.4460431654676, Blast_Score=444, Evalue=1e-125,
Organism=Escherichia coli, GI1789142, Length=542, Percent_Identity=48.7084870848708, Blast_Score=532, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=37.7668308702791, Blast_Score=398, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322563, Length=575, Percent_Identity=39.6521739130435, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6319432, Length=567, Percent_Identity=39.1534391534392, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24664469, Length=555, Percent_Identity=43.0630630630631, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI21357815, Length=499, Percent_Identity=41.0821643286573, Blast_Score=377, Evalue=1e-104,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_HELHP (Q7VGH1)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860881.1
- HSSP:   P17812
- ProteinModelPortal:   Q7VGH1
- SMR:   Q7VGH1
- GeneID:   1493038
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1350
- NMPDR:   fig|235279.1.peg.1350
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- BioCyc:   HHEP235279:HH_1350-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60364; Mature: 60233

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 378-378 ACT_SITE 513-513 ACT_SITE 515-515

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKYIFVTGGVLSSLGKGITSSSIATLLQQSGYSVSILKIDPYINVDPGTMSPLEHGEV
CEEEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCCE
FVTCDGAETDLDIGHYERFLNKDFHKKNNFTTGQIYMSVIENERKGKYLGKTIQIVPHIV
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DEIKSRIKFAGEGSDFLVVELGGTVGDIEGMPYLEAMRQMKHELGGKQVISIHITLIPLM
HHHHHHHEECCCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCEEEEEEEHHHHHH
RAAGELKTKPTQHSVQELRRIGISPQILVARCEKNLDKELKRKLALSCDVDNDSVIVAQD
HHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEC
TQSIYKCPLNFLEEGILTPIARHLELGELKPKMDNWDMLVKKIIAPKSNITIGFVGKYLS
CHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHH
LKESYKSLIESLIHAGANTDTRVNIKWIDSEILVDNLALLYDVDSLLIPGGFGERGIEGK
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHEEECCHHEECCCCCCCCCCCH
LEAIKYARIQQIPLLGICLGMQLSLIEFARNVLGMSEANSIEFDPQTKEPVIYLIENFID
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHC
SQGGVQLRTHTSPMGGTMRLGEYECHIKKGTKLYEAYGKQTLIKERHRHRYEANPHYRAL
CCCCEEEEEECCCCCCCEEECCEEEEECCCCHHHHHHCHHHHHHHHHHCCCCCCCCEEEE
FEKNGMIVSGESNGLIESIELANHPWFVGVQFHPEFTSRLQNPNPVILAFVKETLAHKKT
EECCCEEEECCCCCCEEEHEECCCCEEEEEEECCHHHHHCCCCCCEEEHHHHHHHHCCCC
>Mature Secondary Structure 
AVKYIFVTGGVLSSLGKGITSSSIATLLQQSGYSVSILKIDPYINVDPGTMSPLEHGEV
EEEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCCE
FVTCDGAETDLDIGHYERFLNKDFHKKNNFTTGQIYMSVIENERKGKYLGKTIQIVPHIV
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DEIKSRIKFAGEGSDFLVVELGGTVGDIEGMPYLEAMRQMKHELGGKQVISIHITLIPLM
HHHHHHHEECCCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCEEEEEEEHHHHHH
RAAGELKTKPTQHSVQELRRIGISPQILVARCEKNLDKELKRKLALSCDVDNDSVIVAQD
HHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEC
TQSIYKCPLNFLEEGILTPIARHLELGELKPKMDNWDMLVKKIIAPKSNITIGFVGKYLS
CHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHH
LKESYKSLIESLIHAGANTDTRVNIKWIDSEILVDNLALLYDVDSLLIPGGFGERGIEGK
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHEEECCHHEECCCCCCCCCCCH
LEAIKYARIQQIPLLGICLGMQLSLIEFARNVLGMSEANSIEFDPQTKEPVIYLIENFID
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHC
SQGGVQLRTHTSPMGGTMRLGEYECHIKKGTKLYEAYGKQTLIKERHRHRYEANPHYRAL
CCCCEEEEEECCCCCCCEEECCEEEEECCCCHHHHHHCHHHHHHHHHHCCCCCCCCEEEE
FEKNGMIVSGESNGLIESIELANHPWFVGVQFHPEFTSRLQNPNPVILAFVKETLAHKKT
EECCCEEEECCCCCCEEEHEECCCCEEEEEEECCHHHHHCCCCCCEEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954