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The map label for this gene is recJ

Identifier: 32266850

GI number: 32266850

Start: 1307193

End: 1308761

Strand: Direct

Name: recJ

Synonym: HH1351

Alternate gene names: 32266850

Gene position: 1307193-1308761 (Clockwise)

Preceding gene: 32266849

Following gene: 32266852

Centisome position: 72.66

GC content: 38.88

Gene sequence:

>1569_bases
ATGCGTCAGATTGATAAAGATACCCTTTTACAGATGCTCTTAGAGCGTGATTTAGAGCAAGGTATAGCGACCTTACGTGA
TTTGCCCAAGCCTGATTCACTTACACACGCACTTACAGGCGCACAAAAAGTGGTAGAATGTATGAATAAAGGGCAAGAGA
TTCTTGTGGTAGGGGATTATGATGCTGATGGAATATGTGCAAGTGCGCTAATGTGCCGATTTTTTGAGCGTTTAGGTTAT
GAAAAATTTAGGCTTGTTATTCCTCATCGTTTTATTGATGGCTATGGAGTGAGTGCTGCACTTTTGGAAAAATACGCAAA
TAATGCTTCTATTGTGGTAAGTGTAGATAATGGTATAAACGCATTTTGTGCAGGAGATTGGTGCAAAGAGCACAATATTG
TATTTATCATTACAGACCATCATACACCCGCAAATACATTGCCTCAAGCTGATGTGATTATTGACCCTTATATGGCTGAT
TGCAGCTTTATACAAAAATCAATTTGTGGAGCAGTGGTAGCGTGGTATTTTTGCGCTGCGATAAAACAGATTCTTGGCGC
CTCAATAGATATGGGCGAATTTTTAGAATATCTTGCTTTGGCAAGTATTGCAGATATTATGCCGCTTGTGGGGATTAATC
GCATACTTGTAAAAAAAGGCATAGAGCGACTTCGCAACTCTTCAAGCCCTCTTGCTTTGTTGATACGCTCTAAGCTTAAA
ACTATCAATGCAGAGAATCTTGCATTTTCCATTGTGCCATTGCTTAATTGTGCTGGGCGTATGGCAAATGCAAATTTAGC
TTTGGATTTTTTGCTTGCTCCAAGTGTTGTAGATGCACTTAGAATCTATGATAAACTCTTTGAACTCAATGTTCAGCGCA
AAAGTGTGCAGAGTGAGATTCTATCTCTTGCACAAATGCCAAATATAGAATCTGAACATATTATTGTGAGTTATGGAGAG
GATTGGCACGAAGGCGTTCTTGGTATTGTTGCTTCTTCACTTGCTCAAGAAAAAGGTAAGAGCGCATTTGTATTGAGTTA
TAGCAATGGCATACTTAAGGGGAGTGGGCGCAGTTTTGGAGGGCTTAATCTTATTGCAAGTATCACGCCTTTAAGTAAGC
ATCTTTTGCGTTTTGGTGGGCATAGTGGCGCAGTGGGGATAAGTTTAGAGTTAGAACAATTAGAGGGATTCATAAATGCT
TTAGAATCTGCTCTCATATTTGATGATATAAAAGAAAATGCAATACTTGGTGTGATTGAGAGTGCGAGTATTAATGAGGA
GCTTTTGGCGATTTGTGAGCGCTTTGAACCTTTTGGCGAGGGAAATCCAAAGCCTATATTTTTGTGTGAGAATCTTCAAA
TCCAATCTCTTAAGCCTCTTGGCAGAGATAAAAAGCATTTGAGCTACATATTGAACGATAAAAGCAATGGCGTGTTGCTT
GAAGCCATAGAGTTTTTTGTGCCACAAATGCGTGAAATAGGACAAAGCGGTAATGTGCAATTTGAACTTATGCGCGATGA
TTATAACAATAAAGTCAAAATGAAAATTTTGCAATTTGATGTTTCGTAA

Upstream 100 bases:

>100_bases
CCTGAATTTACTTCGCGATTGCAAAACCCTAATCCTGTCATTTTGGCTTTTGTGAAAGAAACTTTAGCACATAAAAAGAC
ATAAATAAATTGGTATAAAA

Downstream 100 bases:

>100_bases
GGATTCTAAACAATTATTCTCTAAACTGCCATTCTTTTTGTATTTGATGAATATGTGTTAAATCAAGCCCAACAAGCATC
ATTTCTTCTTTATCACCTAA

Product: single-stranded DNA-specific exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MRQIDKDTLLQMLLERDLEQGIATLRDLPKPDSLTHALTGAQKVVECMNKGQEILVVGDYDADGICASALMCRFFERLGY
EKFRLVIPHRFIDGYGVSAALLEKYANNASIVVSVDNGINAFCAGDWCKEHNIVFIITDHHTPANTLPQADVIIDPYMAD
CSFIQKSICGAVVAWYFCAAIKQILGASIDMGEFLEYLALASIADIMPLVGINRILVKKGIERLRNSSSPLALLIRSKLK
TINAENLAFSIVPLLNCAGRMANANLALDFLLAPSVVDALRIYDKLFELNVQRKSVQSEILSLAQMPNIESEHIIVSYGE
DWHEGVLGIVASSLAQEKGKSAFVLSYSNGILKGSGRSFGGLNLIASITPLSKHLLRFGGHSGAVGISLELEQLEGFINA
LESALIFDDIKENAILGVIESASINEELLAICERFEPFGEGNPKPIFLCENLQIQSLKPLGRDKKHLSYILNDKSNGVLL
EAIEFFVPQMREIGQSGNVQFELMRDDYNNKVKMKILQFDVS

Sequences:

>Translated_522_residues
MRQIDKDTLLQMLLERDLEQGIATLRDLPKPDSLTHALTGAQKVVECMNKGQEILVVGDYDADGICASALMCRFFERLGY
EKFRLVIPHRFIDGYGVSAALLEKYANNASIVVSVDNGINAFCAGDWCKEHNIVFIITDHHTPANTLPQADVIIDPYMAD
CSFIQKSICGAVVAWYFCAAIKQILGASIDMGEFLEYLALASIADIMPLVGINRILVKKGIERLRNSSSPLALLIRSKLK
TINAENLAFSIVPLLNCAGRMANANLALDFLLAPSVVDALRIYDKLFELNVQRKSVQSEILSLAQMPNIESEHIIVSYGE
DWHEGVLGIVASSLAQEKGKSAFVLSYSNGILKGSGRSFGGLNLIASITPLSKHLLRFGGHSGAVGISLELEQLEGFINA
LESALIFDDIKENAILGVIESASINEELLAICERFEPFGEGNPKPIFLCENLQIQSLKPLGRDKKHLSYILNDKSNGVLL
EAIEFFVPQMREIGQSGNVQFELMRDDYNNKVKMKILQFDVS
>Mature_522_residues
MRQIDKDTLLQMLLERDLEQGIATLRDLPKPDSLTHALTGAQKVVECMNKGQEILVVGDYDADGICASALMCRFFERLGY
EKFRLVIPHRFIDGYGVSAALLEKYANNASIVVSVDNGINAFCAGDWCKEHNIVFIITDHHTPANTLPQADVIIDPYMAD
CSFIQKSICGAVVAWYFCAAIKQILGASIDMGEFLEYLALASIADIMPLVGINRILVKKGIERLRNSSSPLALLIRSKLK
TINAENLAFSIVPLLNCAGRMANANLALDFLLAPSVVDALRIYDKLFELNVQRKSVQSEILSLAQMPNIESEHIIVSYGE
DWHEGVLGIVASSLAQEKGKSAFVLSYSNGILKGSGRSFGGLNLIASITPLSKHLLRFGGHSGAVGISLELEQLEGFINA
LESALIFDDIKENAILGVIESASINEELLAICERFEPFGEGNPKPIFLCENLQIQSLKPLGRDKKHLSYILNDKSNGVLL
EAIEFFVPQMREIGQSGNVQFELMRDDYNNKVKMKILQFDVS

Specific function: Single-stranded-DNA-specific exonuclease. Required for many types of recombinational events, although the stringency of the requirement for recJ appears to vary with the type of recombinational event monitored and the other recombination gene products whi

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recJ family [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=490, Percent_Identity=30.2040816326531, Blast_Score=223, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 57513; Mature: 57513

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQIDKDTLLQMLLERDLEQGIATLRDLPKPDSLTHALTGAQKVVECMNKGQEILVVGDY
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
DADGICASALMCRFFERLGYEKFRLVIPHRFIDGYGVSAALLEKYANNASIVVSVDNGIN
CCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCC
AFCAGDWCKEHNIVFIITDHHTPANTLPQADVIIDPYMADCSFIQKSICGAVVAWYFCAA
EEECCCCCCCCCEEEEEECCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHH
IKQILGASIDMGEFLEYLALASIADIMPLVGINRILVKKGIERLRNSSSPLALLIRSKLK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
TINAENLAFSIVPLLNCAGRMANANLALDFLLAPSVVDALRIYDKLFELNVQRKSVQSEI
HCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LSLAQMPNIESEHIIVSYGEDWHEGVLGIVASSLAQEKGKSAFVLSYSNGILKGSGRSFG
HHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCC
GLNLIASITPLSKHLLRFGGHSGAVGISLELEQLEGFINALESALIFDDIKENAILGVIE
CHHHHHHCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
SASINEELLAICERFEPFGEGNPKPIFLCENLQIQSLKPLGRDKKHLSYILNDKSNGVLL
CCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCCCCCCHHHHHEEECCCCCCHHH
EAIEFFVPQMREIGQSGNVQFELMRDDYNNKVKMKILQFDVS
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MRQIDKDTLLQMLLERDLEQGIATLRDLPKPDSLTHALTGAQKVVECMNKGQEILVVGDY
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
DADGICASALMCRFFERLGYEKFRLVIPHRFIDGYGVSAALLEKYANNASIVVSVDNGIN
CCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCC
AFCAGDWCKEHNIVFIITDHHTPANTLPQADVIIDPYMADCSFIQKSICGAVVAWYFCAA
EEECCCCCCCCCEEEEEECCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHH
IKQILGASIDMGEFLEYLALASIADIMPLVGINRILVKKGIERLRNSSSPLALLIRSKLK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
TINAENLAFSIVPLLNCAGRMANANLALDFLLAPSVVDALRIYDKLFELNVQRKSVQSEI
HCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LSLAQMPNIESEHIIVSYGEDWHEGVLGIVASSLAQEKGKSAFVLSYSNGILKGSGRSFG
HHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCC
GLNLIASITPLSKHLLRFGGHSGAVGISLELEQLEGFINALESALIFDDIKENAILGVIE
CHHHHHHCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
SASINEELLAICERFEPFGEGNPKPIFLCENLQIQSLKPLGRDKKHLSYILNDKSNGVLL
CCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCCCCCCHHHHHEEECCCCCCHHH
EAIEFFVPQMREIGQSGNVQFELMRDDYNNKVKMKILQFDVS
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]