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The map label for this gene is groEL

Identifier: 32266700

GI number: 32266700

Start: 1168662

End: 1170299

Strand: Direct

Name: groEL

Synonym: HH1201

Alternate gene names: 32266700

Gene position: 1168662-1170299 (Clockwise)

Preceding gene: 32266699

Following gene: 32266703

Centisome position: 64.96

GC content: 38.95

Gene sequence:

>1638_bases
ATGGCAAGCAAAGAAATTAACTTTTCAGATTCGGCAAGGAATAAGCTCTATGAGGGCATTAAGCAATTAAGTGATGCAGT
AAAAGTTACAATGGGACCAAAAGGGCGAAATGTGCTCATTCAAAAAAGTTATGGAGCACCTACGATTACAAAAGATGGTG
TATCTGTGGCAAAAGAAATTGAGCTTGCAGACCCTATTGCAAATATGGGTGCACAGCTTGTAAAAGAAGTGGCAAGCAAA
ACTGCTGATGCAGCAGGAGATGGGACAACAACGGCAACCGTTCTTGCTTATAGCATTTATAAGGAAGGATTAAGAAATAT
TACTGCAGGTGCGAATCCTATTGAAGTAAAACGTGGTATGGATAAAGCAAGTGCAGCAATTATTGAAGAGCTTAAAAAAT
CAAGTAAAAAAATTGGTGGCAAAAGTGATATTGCACAAGTTGCAACTATTTCGGCAAACTCTGATGAAAACATCGGAGCA
TTAATTGCTGAAGCAATGGAAAAAGTTGGTAAAGATGGTGTTATTACCGTTGAGGAAGCAAAAGGTATTAATGATGAGCT
AAGTGTAGTTGAGGGTATGCAATTTGATAGAGGCTATCTTTCTGCGTATTTTGTAACAAATACTGATAAAATGACAGCAC
AGCTTGAAAATGCTTATGTGCTTTTGACAGATAAGAAAATTTCAAATATGAAAGAGATTTTGCCATTGCTTGAGGCAACT
ATGCAAAGTGGCAAACCGCTTTTAATCATTGCTGAAGATATTGAGGGTGAGGCTTTGACAACATTGGTTGTGAATAAATT
GCGTGGAGTGCTTAATGTTTCTGCTGTTAAGGCACCTGGATTTGGCGATAGACGCAAAGCAATGCTTCAAGATATCGCTA
TTCTCACAGGTGGACAAGTTATTAGCGAAGAGCTTGGCAAAACACTTGAAGCAGCAACACTTGCAGATCTAGGAAGTGCA
GCTCGTATCGTGATTGATAAAGATAATACAACAATCGTTGATGGTAAGGGCAAAACAAAAGATGTTAAAGATAGAATCGC
ACAAATCAAAACTGAAATTGAAAACACTACAAGCGATTATGATAGAGAAAAGCTCCAAGAGAGATTAGCAAAATTAAGCG
GTGGTGTGGCAGTTATTAAGGTTGGTGCAGCAAGTGAAGTAGAGATGAAAGAGAAAAAAGACCGCGTAGATGATGCACTT
TCTGCAACAAAAGCAGCGGTTGATGAGGGAATCGTTATTGGCGGTGGTTCTGCCCTTATTCGTGCTTCACAAAAAGTTAA
ACTTAAGCTTGAAGGTGATGAAGCAATTGGTTATGATATTATCAAACGCGCAATTAAAGCTCCTCTTGCACAAATTGCGA
CAAATGCAGGATATGATGCAGGTGTGGTTGTGAATGAGGTAGAGAAAAATTCTAAAGATGGCTTTGGGTTCAATGCGACT
ACAGGTGAATATGTAGATATGTTTAAAGAGGGCATTATTGACCCACTCAAAGTTACACGTGTGGCGTTACAAAATGCAGT
ATCAGTTTCAAGTCTGCTTTTAACAACTGAAGCAACAATCAATGAAATCAAAGAAGATAAACCAGCTCCAGCAATGCCAG
ATATGGGTGGAATGGGCGGTATGGGTGGAATGATGTAA

Upstream 100 bases:

>100_bases
CAAGCTAGATTCAAAGGAGTTTATTGTCCTAGAGCTAGATGATATTTTGGGCGTTATTGAAAAATAAATTTTAGAATCTT
AAACATTAAAGGAGTAAAAA

Downstream 100 bases:

>100_bases
TCCCCCTTGCTTTTTGAGTTTATAGAATCTGTGTAGATTCTATAAACTCCTTTTTTACTAGCTTAAACCTTTCTCAAATA
GAGCATTTGCTTCCTGCATA

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MASKEINFSDSARNKLYEGIKQLSDAVKVTMGPKGRNVLIQKSYGAPTITKDGVSVAKEIELADPIANMGAQLVKEVASK
TADAAGDGTTTATVLAYSIYKEGLRNITAGANPIEVKRGMDKASAAIIEELKKSSKKIGGKSDIAQVATISANSDENIGA
LIAEAMEKVGKDGVITVEEAKGINDELSVVEGMQFDRGYLSAYFVTNTDKMTAQLENAYVLLTDKKISNMKEILPLLEAT
MQSGKPLLIIAEDIEGEALTTLVVNKLRGVLNVSAVKAPGFGDRRKAMLQDIAILTGGQVISEELGKTLEAATLADLGSA
ARIVIDKDNTTIVDGKGKTKDVKDRIAQIKTEIENTTSDYDREKLQERLAKLSGGVAVIKVGAASEVEMKEKKDRVDDAL
SATKAAVDEGIVIGGGSALIRASQKVKLKLEGDEAIGYDIIKRAIKAPLAQIATNAGYDAGVVVNEVEKNSKDGFGFNAT
TGEYVDMFKEGIIDPLKVTRVALQNAVSVSSLLLTTEATINEIKEDKPAPAMPDMGGMGGMGGMM

Sequences:

>Translated_545_residues
MASKEINFSDSARNKLYEGIKQLSDAVKVTMGPKGRNVLIQKSYGAPTITKDGVSVAKEIELADPIANMGAQLVKEVASK
TADAAGDGTTTATVLAYSIYKEGLRNITAGANPIEVKRGMDKASAAIIEELKKSSKKIGGKSDIAQVATISANSDENIGA
LIAEAMEKVGKDGVITVEEAKGINDELSVVEGMQFDRGYLSAYFVTNTDKMTAQLENAYVLLTDKKISNMKEILPLLEAT
MQSGKPLLIIAEDIEGEALTTLVVNKLRGVLNVSAVKAPGFGDRRKAMLQDIAILTGGQVISEELGKTLEAATLADLGSA
ARIVIDKDNTTIVDGKGKTKDVKDRIAQIKTEIENTTSDYDREKLQERLAKLSGGVAVIKVGAASEVEMKEKKDRVDDAL
SATKAAVDEGIVIGGGSALIRASQKVKLKLEGDEAIGYDIIKRAIKAPLAQIATNAGYDAGVVVNEVEKNSKDGFGFNAT
TGEYVDMFKEGIIDPLKVTRVALQNAVSVSSLLLTTEATINEIKEDKPAPAMPDMGGMGGMGGMM
>Mature_544_residues
ASKEINFSDSARNKLYEGIKQLSDAVKVTMGPKGRNVLIQKSYGAPTITKDGVSVAKEIELADPIANMGAQLVKEVASKT
ADAAGDGTTTATVLAYSIYKEGLRNITAGANPIEVKRGMDKASAAIIEELKKSSKKIGGKSDIAQVATISANSDENIGAL
IAEAMEKVGKDGVITVEEAKGINDELSVVEGMQFDRGYLSAYFVTNTDKMTAQLENAYVLLTDKKISNMKEILPLLEATM
QSGKPLLIIAEDIEGEALTTLVVNKLRGVLNVSAVKAPGFGDRRKAMLQDIAILTGGQVISEELGKTLEAATLADLGSAA
RIVIDKDNTTIVDGKGKTKDVKDRIAQIKTEIENTTSDYDREKLQERLAKLSGGVAVIKVGAASEVEMKEKKDRVDDALS
ATKAAVDEGIVIGGGSALIRASQKVKLKLEGDEAIGYDIIKRAIKAPLAQIATNAGYDAGVVVNEVEKNSKDGFGFNATT
GEYVDMFKEGIIDPLKVTRVALQNAVSVSSLLLTTEATINEIKEDKPAPAMPDMGGMGGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=531, Percent_Identity=51.0357815442561, Blast_Score=518, Evalue=1e-147,
Organism=Homo sapiens, GI31542947, Length=531, Percent_Identity=51.0357815442561, Blast_Score=518, Evalue=1e-147,
Organism=Escherichia coli, GI1790586, Length=532, Percent_Identity=61.8421052631579, Blast_Score=637, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=528, Percent_Identity=50.7575757575758, Blast_Score=514, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI193210679, Length=208, Percent_Identity=50.4807692307692, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17564182, Length=561, Percent_Identity=23.8859180035651, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=52.0992366412214, Blast_Score=524, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6322350, Length=559, Percent_Identity=22.1824686940966, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322524, Length=123, Percent_Identity=30.8943089430894, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322446, Length=533, Percent_Identity=21.9512195121951, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24641193, Length=532, Percent_Identity=52.6315789473684, Blast_Score=530, Evalue=1e-151,
Organism=Drosophila melanogaster, GI24641191, Length=532, Percent_Identity=52.6315789473684, Blast_Score=530, Evalue=1e-151,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=49.5238095238095, Blast_Score=501, Evalue=1e-142,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=49.5238095238095, Blast_Score=501, Evalue=1e-142,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=49.5238095238095, Blast_Score=501, Evalue=1e-142,
Organism=Drosophila melanogaster, GI17864606, Length=541, Percent_Identity=45.1016635859519, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24584129, Length=535, Percent_Identity=34.5794392523364, Blast_Score=301, Evalue=6e-82,
Organism=Drosophila melanogaster, GI19921262, Length=535, Percent_Identity=34.5794392523364, Blast_Score=301, Evalue=6e-82,
Organism=Drosophila melanogaster, GI24645179, Length=517, Percent_Identity=20.5029013539652, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_HELHP (Q7U317)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860732.1
- ProteinModelPortal:   Q7U317
- SMR:   Q7U317
- GeneID:   1492889
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1201
- NMPDR:   fig|235279.1.peg.1201
- HOGENOM:   HBG625289
- OMA:   DKPAAGM
- ProtClustDB:   PRK00013
- BioCyc:   HHEP235279:HH_1201-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57652; Mature: 57521

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKEINFSDSARNKLYEGIKQLSDAVKVTMGPKGRNVLIQKSYGAPTITKDGVSVAKEI
CCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHH
ELADPIANMGAQLVKEVASKTADAAGDGTTTATVLAYSIYKEGLRNITAGANPIEVKRGM
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCC
DKASAAIIEELKKSSKKIGGKSDIAQVATISANSDENIGALIAEAMEKVGKDGVITVEEA
CHHHHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCC
KGINDELSVVEGMQFDRGYLSAYFVTNTDKMTAQLENAYVLLTDKKISNMKEILPLLEAT
CCCCHHHHHHHCCCCCCCCEEEEEEECCHHHHHEECCEEEEEECHHHHHHHHHHHHHHHH
MQSGKPLLIIAEDIEGEALTTLVVNKLRGVLNVSAVKAPGFGDRRKAMLQDIAILTGGQV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEHEEECCCCCHHHHHHHHHHHHHCCCHH
ISEELGKTLEAATLADLGSAARIVIDKDNTTIVDGKGKTKDVKDRIAQIKTEIENTTSDY
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCHH
DREKLQERLAKLSGGVAVIKVGAASEVEMKEKKDRVDDALSATKAAVDEGIVIGGGSALI
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHE
RASQKVKLKLEGDEAIGYDIIKRAIKAPLAQIATNAGYDAGVVVNEVEKNSKDGFGFNAT
ECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCCCCCCCCCC
TGEYVDMFKEGIIDPLKVTRVALQNAVSVSSLLLTTEATINEIKEDKPAPAMPDMGGMGG
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
MGGMM
CCCCC
>Mature Secondary Structure 
ASKEINFSDSARNKLYEGIKQLSDAVKVTMGPKGRNVLIQKSYGAPTITKDGVSVAKEI
CCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHH
ELADPIANMGAQLVKEVASKTADAAGDGTTTATVLAYSIYKEGLRNITAGANPIEVKRGM
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCC
DKASAAIIEELKKSSKKIGGKSDIAQVATISANSDENIGALIAEAMEKVGKDGVITVEEA
CHHHHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCC
KGINDELSVVEGMQFDRGYLSAYFVTNTDKMTAQLENAYVLLTDKKISNMKEILPLLEAT
CCCCHHHHHHHCCCCCCCCEEEEEEECCHHHHHEECCEEEEEECHHHHHHHHHHHHHHHH
MQSGKPLLIIAEDIEGEALTTLVVNKLRGVLNVSAVKAPGFGDRRKAMLQDIAILTGGQV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEHEEECCCCCHHHHHHHHHHHHHCCCHH
ISEELGKTLEAATLADLGSAARIVIDKDNTTIVDGKGKTKDVKDRIAQIKTEIENTTSDY
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCHH
DREKLQERLAKLSGGVAVIKVGAASEVEMKEKKDRVDDALSATKAAVDEGIVIGGGSALI
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHE
RASQKVKLKLEGDEAIGYDIIKRAIKAPLAQIATNAGYDAGVVVNEVEKNSKDGFGFNAT
ECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCCCCCCCCCC
TGEYVDMFKEGIIDPLKVTRVALQNAVSVSSLLLTTEATINEIKEDKPAPAMPDMGGMGG
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
MGGMM
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954