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The map label for this gene is pgk

Identifier: 32265826

GI number: 32265826

Start: 324933

End: 326141

Strand: Direct

Name: pgk

Synonym: HH0327

Alternate gene names: 32265826

Gene position: 324933-326141 (Clockwise)

Preceding gene: 32265818

Following gene: 32265827

Centisome position: 18.06

GC content: 39.95

Gene sequence:

>1209_bases
ATGAAGGAAACTGGAATCGAATTAATGAAAAATACAAAAAGTGTGCGTGATATTGACGTCAAAGACAAAAGAGTGCTTAT
TCGCGTGGATTTTAATGTGCCAATGGACGAGGATTTTGATATTTCAGATGATACGCGTATCCGCGAAGCACTACCCACTA
TTAATTATTGCATTGATAATCACGCACAAAATATAGTGCTTGTCAGCCATTTAGGGCGTCCCAAAGGACGCAATGCAGAG
TTTTCACTCAAACACGTGCTTAAACGTGTAGAGCGACTTTTAGGACGCGATATAGCTTTCGCAGAGACAATAGAAAATGT
AGAAAATCTTCAACAACAAAGCAAGAGTGGAAGTGTGATTTTACTTGAAAATATTAGATTCTATGAGGGAGAGGAAAAAA
ATGATGAGGAACTCTCTACTAAACTCGCCTCTATTTGTGATGTGTATATCAATGATGCCTTTGGCACAAGCCATCGCGCA
CATTCAAGCACCTGTGGCATTGCTAAATATGCCAAAGAGTGTGTAGCAGGATTATTGCTCAAAAAAGAAATTGATTCATT
TGCTAAAGCAATGGCAAATCCACTTAAACCGGTGTTACTTATCGTTGGCGGAAGCAAGGTAAGCTCTAAACTCGCACTCT
TATATAATATCCTTGATGTGGTGGATAAAATCATCATTGGTGGTGCGATGAGCAATACTTTCCTTAAAGCACGAGGTTTT
GATATGCAAAAATCCCTTGTGGAAGATGATTTAGTGAGTGAAGCACGTAAGATTTTGGATAAAGCAAAAGAAAAAAAAGT
CAAAATCTACCTTCCTGTAGATGTTGTAAGCACTGATGATATTAAAGAACATCGCCATATCAAAATCACGCCTGCACAAG
ATATACCAGAGGGCTTTATGGCAGTAGATATGGGACCTGCAACAAGTAAGCTCTTTAGTGAAGTGGTAAGGGATTCTCAA
ACAATTATTTGGAATGGACCGCTTGGCATTTATGAAATACAAGCTTTTTCACGCGGCACTTTTAACCTTGCCCACGCTGT
GAGCGATACTTATGCCTTTAGTCTTATTGGTGGTGGCGATACTGCTGATGCAATAGACAAGGCAGGAGAGCGAGACAATA
TGAGCTTTATTTCCACGGGTGGTGGAGCAAGTTTGGAGCTACTTGAAGGGAAAATTCTTCCTGCATTTGAGGTGCTTGAG
CGCAAATAA

Upstream 100 bases:

>100_bases
ATAACATTGTTTATTAAGGATAATACAAAGCACTCATAACGCATATATAAAAATATGATAAAATACCGCATTTATTTACA
GAATCTAATAAGGAGCGTAG

Downstream 100 bases:

>100_bases
TGAATCTCTTTGCTCTTAGTCTTGAAGCTCTCCACGCTCCAAGCATAGAATCTAAACTTGCTCTCCTTGAAGTAGTTGCA
TCTCAAAAACATCTGCTCTG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKETGIELMKNTKSVRDIDVKDKRVLIRVDFNVPMDEDFDISDDTRIREALPTINYCIDNHAQNIVLVSHLGRPKGRNAE
FSLKHVLKRVERLLGRDIAFAETIENVENLQQQSKSGSVILLENIRFYEGEEKNDEELSTKLASICDVYINDAFGTSHRA
HSSTCGIAKYAKECVAGLLLKKEIDSFAKAMANPLKPVLLIVGGSKVSSKLALLYNILDVVDKIIIGGAMSNTFLKARGF
DMQKSLVEDDLVSEARKILDKAKEKKVKIYLPVDVVSTDDIKEHRHIKITPAQDIPEGFMAVDMGPATSKLFSEVVRDSQ
TIIWNGPLGIYEIQAFSRGTFNLAHAVSDTYAFSLIGGGDTADAIDKAGERDNMSFISTGGGASLELLEGKILPAFEVLE
RK

Sequences:

>Translated_402_residues
MKETGIELMKNTKSVRDIDVKDKRVLIRVDFNVPMDEDFDISDDTRIREALPTINYCIDNHAQNIVLVSHLGRPKGRNAE
FSLKHVLKRVERLLGRDIAFAETIENVENLQQQSKSGSVILLENIRFYEGEEKNDEELSTKLASICDVYINDAFGTSHRA
HSSTCGIAKYAKECVAGLLLKKEIDSFAKAMANPLKPVLLIVGGSKVSSKLALLYNILDVVDKIIIGGAMSNTFLKARGF
DMQKSLVEDDLVSEARKILDKAKEKKVKIYLPVDVVSTDDIKEHRHIKITPAQDIPEGFMAVDMGPATSKLFSEVVRDSQ
TIIWNGPLGIYEIQAFSRGTFNLAHAVSDTYAFSLIGGGDTADAIDKAGERDNMSFISTGGGASLELLEGKILPAFEVLE
RK
>Mature_402_residues
MKETGIELMKNTKSVRDIDVKDKRVLIRVDFNVPMDEDFDISDDTRIREALPTINYCIDNHAQNIVLVSHLGRPKGRNAE
FSLKHVLKRVERLLGRDIAFAETIENVENLQQQSKSGSVILLENIRFYEGEEKNDEELSTKLASICDVYINDAFGTSHRA
HSSTCGIAKYAKECVAGLLLKKEIDSFAKAMANPLKPVLLIVGGSKVSSKLALLYNILDVVDKIIIGGAMSNTFLKARGF
DMQKSLVEDDLVSEARKILDKAKEKKVKIYLPVDVVSTDDIKEHRHIKITPAQDIPEGFMAVDMGPATSKLFSEVVRDSQ
TIIWNGPLGIYEIQAFSRGTFNLAHAVSDTYAFSLIGGGDTADAIDKAGERDNMSFISTGGGASLELLEGKILPAFEVLE
RK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=415, Percent_Identity=42.4096385542169, Blast_Score=318, Evalue=6e-87,
Organism=Homo sapiens, GI31543397, Length=406, Percent_Identity=43.1034482758621, Blast_Score=317, Evalue=2e-86,
Organism=Escherichia coli, GI1789294, Length=388, Percent_Identity=42.0103092783505, Blast_Score=309, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17508823, Length=412, Percent_Identity=42.2330097087379, Blast_Score=311, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6319857, Length=415, Percent_Identity=44.578313253012, Blast_Score=320, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24581203, Length=412, Percent_Identity=42.4757281553398, Blast_Score=304, Evalue=8e-83,
Organism=Drosophila melanogaster, GI17136394, Length=412, Percent_Identity=41.2621359223301, Blast_Score=288, Evalue=6e-78,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_HELHP (Q7VJB6)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_859858.1
- ProteinModelPortal:   Q7VJB6
- SMR:   Q7VJB6
- GeneID:   1493381
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH0327
- NMPDR:   fig|235279.1.peg.327
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   HHEP235279:HH_0327-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 44465; Mature: 44465

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 46-46 BINDING 126-126 BINDING 159-159 BINDING 210-210 BINDING 332-332

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETGIELMKNTKSVRDIDVKDKRVLIRVDFNVPMDEDFDISDDTRIREALPTINYCIDN
CCCCHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
HAQNIVLVSHLGRPKGRNAEFSLKHVLKRVERLLGRDIAFAETIENVENLQQQSKSGSVI
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEE
LLENIRFYEGEEKNDEELSTKLASICDVYINDAFGTSHRAHSSTCGIAKYAKECVAGLLL
EEECCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KKEIDSFAKAMANPLKPVLLIVGGSKVSSKLALLYNILDVVDKIIIGGAMSNTFLKARGF
HHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHCCC
DMQKSLVEDDLVSEARKILDKAKEKKVKIYLPVDVVSTDDIKEHRHIKITPAQDIPEGFM
CHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCE
AVDMGPATSKLFSEVVRDSQTIIWNGPLGIYEIQAFSRGTFNLAHAVSDTYAFSLIGGGD
EEECCCHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCHHHHHHHCCCEEEEEECCCC
TADAIDKAGERDNMSFISTGGGASLELLEGKILPAFEVLERK
CHHHHHHCCCCCCCEEEECCCCCEEEEECCCCCCHHHHHCCC
>Mature Secondary Structure
MKETGIELMKNTKSVRDIDVKDKRVLIRVDFNVPMDEDFDISDDTRIREALPTINYCIDN
CCCCHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
HAQNIVLVSHLGRPKGRNAEFSLKHVLKRVERLLGRDIAFAETIENVENLQQQSKSGSVI
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEE
LLENIRFYEGEEKNDEELSTKLASICDVYINDAFGTSHRAHSSTCGIAKYAKECVAGLLL
EEECCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KKEIDSFAKAMANPLKPVLLIVGGSKVSSKLALLYNILDVVDKIIIGGAMSNTFLKARGF
HHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHCCC
DMQKSLVEDDLVSEARKILDKAKEKKVKIYLPVDVVSTDDIKEHRHIKITPAQDIPEGFM
CHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCE
AVDMGPATSKLFSEVVRDSQTIIWNGPLGIYEIQAFSRGTFNLAHAVSDTYAFSLIGGGD
EEECCCHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCHHHHHHHCCCEEEEEECCCC
TADAIDKAGERDNMSFISTGGGASLELLEGKILPAFEVLERK
CHHHHHHCCCCCCCEEEECCCCCEEEEECCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954