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The map label for this gene is lacZ [H]

Identifier: 29350092

GI number: 29350092

Start: 6145069

End: 6147756

Strand: Reverse

Name: lacZ [H]

Synonym: BT_4684

Alternate gene names: 29350092

Gene position: 6147756-6145069 (Counterclockwise)

Preceding gene: 29350093

Following gene: 29350091

Centisome position: 98.2

GC content: 45.5

Gene sequence:

>2688_bases
ATGAAAAGTACTTCAACATGGTGGAAAATCTTACTCAGTGGTCTGTTATTGGCTGGAACACTGGTAGCTAACGCCGACAC
TTACCAGCCATCTTACTCCACAGCAGGCTTTTATCAGCTTTCCAACACAGGCCGCACAGTGTATAACATGAATCCTGCCT
GGCGATTTTATAAAGGCAATATCGAAGGAGCGGAACAGCCGAGATTCAATGATAAAGACTGGAACATCGTATCATTACCC
GATGGCATAGAATATCTCCCCACCGAAGCGAGCGGATGCATCAATTATCAGGGGGAAATCTGGTATCGCAAACACTTCAC
TCCCAAGGAGAGTTGGAAAGGAAAACAACAATTCCTCCACTTCGAAGCCATTATGGGAAAATCAAAAATATGGGTGAACG
GAAAGTTGCTGAAAGAGCATTTCGGTGGTTTTCTACCTGTCATCGTAGATGTCACTTCCAGTCTGAAGTATGGAGAAGAC
AATGTTATCACCGTATGGGCAGACAACAGTGACGATCCCTCCTACCCTCCCGGCAAACCGCAAGATGCACTGGACTTTGC
ATATTTTGGAGGAATTTACCGAGATTGCTGGATGATAGTTCACAACAAAGTATTCATTACCGATCCGAATTATGAGAATG
AGACAGCCGGAGGCGGATTATTTGTCTCTTTCGACAAAGTTTCCGAGCAATCCGCACAGTTGAAACTGGATGCACATATA
CGCAACACTTCCGGAAAATCCTTCAGTGGTAAAATAATCTATGAACTCTATGACCGCGACGACAAACGTGTTCTCTCTAA
GGAGACCGGTTTGTCGGTAAGCAAGGATCTTGCGAAACAAATTTCTTGCAAAGTGACCGTAGAAACACCTCATTTATGGT
CACCCGACAGCCCCTATCTTTACAAACTACACATTTATATAAAAGACAAAGCCGGTAACACCATCGACGGATATTACCGC
CGTATCGGCATTCGCAGCATTGAATTTAAAGGAAAAGACGGATTCTGGCTAAATGGCAAACCTTACCCCTATCCTCTGAT
GGGAGCCAACCGTCATCAAGATTTTGCAGTGATTGGTAACGCCTTACCCAACAGCCTTCATTGGCGGGATGCGAAGAAGT
TGCGTGATGCCGGAATGCGTGTTATCCGTAACGCCCATTATCCGCAAGATCCGGCGTTTATGGATGCTTGTGATGAATTA
GGACTGTTTGTTATTGTCAACACACCGGGATGGCAATTCTGGAATGATCAGCCGATATTTGCTCAGAGAGTATATAGTGA
TATCCGCAATATGGTTCGCCGCGACCGCAATCACCCCAGTGTATGGATGTGGGAACCGATTCTGAACGAAACATGGTATC
CGGCTGACTTTGCTAAAAATGTAGTGGACATTCTCAATGAAGAATATCCGTATCCTTATTGCTATGCCGGATGTGATGTA
ACCGCACGTGGACACGAATACTTTCCGATACACTTCACCCATCCGATGAACGGAGGTGGAGGAGCCTTTAATACCGAAAA
TCTGGATCCGAAAATCAGTTATTTCACCCGCGAATGGGGAGATAATGTGGATGACTGGAACTCTCACAACTCACCAAGCC
GGGTCAATCGCGGCTGGGGAGAAGTACCGATGCTGATTCAGGCACAGGGATATGCCAAAAACGATTACCAACTCACCAGT
TATGATGGACTATACAGAACTTCAAGACAACATATGGGCGGTTGCCTGTGGCACTCGTTCGATCACCAGAGAGGATATCA
TCCTGACCCGTTTTACGGAGGTATCATGGATGCCTTCCGACAACCTAAACTATCTTATTATATGTTCTGTTCCCAGCGTC
CTGCCGAACCCAACAAAGAATTGATCGCAGATAATGGACCGATGATTTACATAGCCAATGCCATGACCCCATTCTCTCCG
AAAGATGTAACGATATATAGCAACTGTGACGAAGTTCGCCTCACGTATTGCAAGGGAGGCAAAGAATATACTTATCATAA
GCCGGCAAATGAAAGCGGAATGCCCTCACCCGTCATCACATTCAAGGATGTATTTGATGTCATGTATGATAAAAAGCTGT
CACGCCAAAAGAAACAGGCAGATTCTTACCTTCTGGCAGAAGGATTAATGGACGGAAAAGTGGTTGCGACACATAAAGTA
ACACCCACACGCCGCCCGTCAAAACTGTTGCTTTGGGCAGATGACGAGAAAGTACAGATGAAAGCGGACGGATCGGACAT
CGTGACGGTCATTGCAGCCATCGCAGATGAAAACGGAAACATCAAGCGTTTGAATAATTATGAAGTAAAGTTCGAAATCG
AAGGGCAGGGCCAACTAGTTGCCAATGAAGAAACATTCACCAATCCACGTCCGGTACTTTGGGGAACTGCTCCGGTGCTG
GTAAGGTCCACCACTACACCGGGAAAAATTAAAATCCGTGCATCAGTGGTATGGCAAGGCAAACATACGCCGGTTCCGGC
AGAACTGATTATTCCTACTTTTCCATCAGAACATACGCTGGTAGCAGACAAAGATGAACTGACACAAGCACAATCCGCAA
GCAAAGATGCCGGAAATAAAATAAATGCCGCTCCATCCGATTGTGAGAAACGTGTACTTGAATTACAACAGGAACTGAAC
CGCCTGAAACTGAAAGAAGTCGAAAAACAACAGAGTGACTTTGAATAA

Upstream 100 bases:

>100_bases
TTGGAAAATTTGTACCTTGGCGGGACGAAGTTCTAATGCAAAACAAAACAGTCTGACATTAATATATTAAAGATAGATCA
ATCAAGCAAATCTAAGAATT

Downstream 100 bases:

>100_bases
TGCGCATAGTTCTATCTATAAACAGAAAAAACACAAACTTATTAAAATACAGTTATGATAAATAGAATAGTATATACTTC
ATTTTTATTAGGAGCGAGTT

Product: beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 895; Mature: 895

Protein sequence:

>895_residues
MKSTSTWWKILLSGLLLAGTLVANADTYQPSYSTAGFYQLSNTGRTVYNMNPAWRFYKGNIEGAEQPRFNDKDWNIVSLP
DGIEYLPTEASGCINYQGEIWYRKHFTPKESWKGKQQFLHFEAIMGKSKIWVNGKLLKEHFGGFLPVIVDVTSSLKYGED
NVITVWADNSDDPSYPPGKPQDALDFAYFGGIYRDCWMIVHNKVFITDPNYENETAGGGLFVSFDKVSEQSAQLKLDAHI
RNTSGKSFSGKIIYELYDRDDKRVLSKETGLSVSKDLAKQISCKVTVETPHLWSPDSPYLYKLHIYIKDKAGNTIDGYYR
RIGIRSIEFKGKDGFWLNGKPYPYPLMGANRHQDFAVIGNALPNSLHWRDAKKLRDAGMRVIRNAHYPQDPAFMDACDEL
GLFVIVNTPGWQFWNDQPIFAQRVYSDIRNMVRRDRNHPSVWMWEPILNETWYPADFAKNVVDILNEEYPYPYCYAGCDV
TARGHEYFPIHFTHPMNGGGGAFNTENLDPKISYFTREWGDNVDDWNSHNSPSRVNRGWGEVPMLIQAQGYAKNDYQLTS
YDGLYRTSRQHMGGCLWHSFDHQRGYHPDPFYGGIMDAFRQPKLSYYMFCSQRPAEPNKELIADNGPMIYIANAMTPFSP
KDVTIYSNCDEVRLTYCKGGKEYTYHKPANESGMPSPVITFKDVFDVMYDKKLSRQKKQADSYLLAEGLMDGKVVATHKV
TPTRRPSKLLLWADDEKVQMKADGSDIVTVIAAIADENGNIKRLNNYEVKFEIEGQGQLVANEETFTNPRPVLWGTAPVL
VRSTTTPGKIKIRASVVWQGKHTPVPAELIIPTFPSEHTLVADKDELTQAQSASKDAGNKINAAPSDCEKRVLELQQELN
RLKLKEVEKQQSDFE

Sequences:

>Translated_895_residues
MKSTSTWWKILLSGLLLAGTLVANADTYQPSYSTAGFYQLSNTGRTVYNMNPAWRFYKGNIEGAEQPRFNDKDWNIVSLP
DGIEYLPTEASGCINYQGEIWYRKHFTPKESWKGKQQFLHFEAIMGKSKIWVNGKLLKEHFGGFLPVIVDVTSSLKYGED
NVITVWADNSDDPSYPPGKPQDALDFAYFGGIYRDCWMIVHNKVFITDPNYENETAGGGLFVSFDKVSEQSAQLKLDAHI
RNTSGKSFSGKIIYELYDRDDKRVLSKETGLSVSKDLAKQISCKVTVETPHLWSPDSPYLYKLHIYIKDKAGNTIDGYYR
RIGIRSIEFKGKDGFWLNGKPYPYPLMGANRHQDFAVIGNALPNSLHWRDAKKLRDAGMRVIRNAHYPQDPAFMDACDEL
GLFVIVNTPGWQFWNDQPIFAQRVYSDIRNMVRRDRNHPSVWMWEPILNETWYPADFAKNVVDILNEEYPYPYCYAGCDV
TARGHEYFPIHFTHPMNGGGGAFNTENLDPKISYFTREWGDNVDDWNSHNSPSRVNRGWGEVPMLIQAQGYAKNDYQLTS
YDGLYRTSRQHMGGCLWHSFDHQRGYHPDPFYGGIMDAFRQPKLSYYMFCSQRPAEPNKELIADNGPMIYIANAMTPFSP
KDVTIYSNCDEVRLTYCKGGKEYTYHKPANESGMPSPVITFKDVFDVMYDKKLSRQKKQADSYLLAEGLMDGKVVATHKV
TPTRRPSKLLLWADDEKVQMKADGSDIVTVIAAIADENGNIKRLNNYEVKFEIEGQGQLVANEETFTNPRPVLWGTAPVL
VRSTTTPGKIKIRASVVWQGKHTPVPAELIIPTFPSEHTLVADKDELTQAQSASKDAGNKINAAPSDCEKRVLELQQELN
RLKLKEVEKQQSDFE
>Mature_895_residues
MKSTSTWWKILLSGLLLAGTLVANADTYQPSYSTAGFYQLSNTGRTVYNMNPAWRFYKGNIEGAEQPRFNDKDWNIVSLP
DGIEYLPTEASGCINYQGEIWYRKHFTPKESWKGKQQFLHFEAIMGKSKIWVNGKLLKEHFGGFLPVIVDVTSSLKYGED
NVITVWADNSDDPSYPPGKPQDALDFAYFGGIYRDCWMIVHNKVFITDPNYENETAGGGLFVSFDKVSEQSAQLKLDAHI
RNTSGKSFSGKIIYELYDRDDKRVLSKETGLSVSKDLAKQISCKVTVETPHLWSPDSPYLYKLHIYIKDKAGNTIDGYYR
RIGIRSIEFKGKDGFWLNGKPYPYPLMGANRHQDFAVIGNALPNSLHWRDAKKLRDAGMRVIRNAHYPQDPAFMDACDEL
GLFVIVNTPGWQFWNDQPIFAQRVYSDIRNMVRRDRNHPSVWMWEPILNETWYPADFAKNVVDILNEEYPYPYCYAGCDV
TARGHEYFPIHFTHPMNGGGGAFNTENLDPKISYFTREWGDNVDDWNSHNSPSRVNRGWGEVPMLIQAQGYAKNDYQLTS
YDGLYRTSRQHMGGCLWHSFDHQRGYHPDPFYGGIMDAFRQPKLSYYMFCSQRPAEPNKELIADNGPMIYIANAMTPFSP
KDVTIYSNCDEVRLTYCKGGKEYTYHKPANESGMPSPVITFKDVFDVMYDKKLSRQKKQADSYLLAEGLMDGKVVATHKV
TPTRRPSKLLLWADDEKVQMKADGSDIVTVIAAIADENGNIKRLNNYEVKFEIEGQGQLVANEETFTNPRPVLWGTAPVL
VRSTTTPGKIKIRASVVWQGKHTPVPAELIIPTFPSEHTLVADKDELTQAQSASKDAGNKINAAPSDCEKRVLELQQELN
RLKLKEVEKQQSDFE

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=386, Percent_Identity=24.8704663212435, Blast_Score=103, Evalue=9e-22,
Organism=Escherichia coli, GI48994920, Length=363, Percent_Identity=27.8236914600551, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1787903, Length=388, Percent_Identity=29.1237113402062, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1786539, Length=413, Percent_Identity=23.0024213075061, Blast_Score=94, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17510825, Length=472, Percent_Identity=23.0932203389831, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI62471735, Length=446, Percent_Identity=26.2331838565022, Blast_Score=132, Evalue=9e-31,
Organism=Drosophila melanogaster, GI19922582, Length=399, Percent_Identity=27.5689223057644, Blast_Score=132, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24655438, Length=473, Percent_Identity=26.0042283298097, Blast_Score=132, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24651745, Length=453, Percent_Identity=24.5033112582781, Blast_Score=124, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 101988; Mature: 101988

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTSTWWKILLSGLLLAGTLVANADTYQPSYSTAGFYQLSNTGRTVYNMNPAWRFYKGN
CCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCEEEECCCCCEEEECCCCEEEEECC
IEGAEQPRFNDKDWNIVSLPDGIEYLPTEASGCINYQGEIWYRKHFTPKESWKGKQQFLH
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEECCCCEEEEECCCCHHHCCCHHHHEE
FEAIMGKSKIWVNGKLLKEHFGGFLPVIVDVTSSLKYGEDNVITVWADNSDDPSYPPGKP
HHHHCCCCEEEECCEEHHHHCCCCEEEEEEHHHCCCCCCCCEEEEEECCCCCCCCCCCCC
QDALDFAYFGGIYRDCWMIVHNKVFITDPNYENETAGGGLFVSFDKVSEQSAQLKLDAHI
CHHHHHHHHHHHHHHHEEEEECEEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEE
RNTSGKSFSGKIIYELYDRDDKRVLSKETGLSVSKDLAKQISCKVTVETPHLWSPDSPYL
ECCCCCCCCCEEEEEEECCCCHHHHHHHCCCCHHHHHHHCCEEEEEEECCCCCCCCCCEE
YKLHIYIKDKAGNTIDGYYRRIGIRSIEFKGKDGFWLNGKPYPYPLMGANRHQDFAVIGN
EEEEEEEECCCCCCHHHHHHHHCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEECC
ALPNSLHWRDAKKLRDAGMRVIRNAHYPQDPAFMDACDELGLFVIVNTPGWQFWNDQPIF
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCHH
AQRVYSDIRNMVRRDRNHPSVWMWEPILNETWYPADFAKNVVDILNEEYPYPYCYAGCDV
HHHHHHHHHHHHHHCCCCCCEEEECHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCE
TARGHEYFPIHFTHPMNGGGGAFNTENLDPKISYFTREWGDNVDDWNSHNSPSRVNRGWG
EECCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCC
EVPMLIQAQGYAKNDYQLTSYDGLYRTSRQHMGGCLWHSFDHQRGYHPDPFYGGIMDAFR
CCCEEEEECCCCCCCCEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHC
QPKLSYYMFCSQRPAEPNKELIADNGPMIYIANAMTPFSPKDVTIYSNCDEVRLTYCKGG
CCCCEEEEEECCCCCCCCHHEEECCCCEEEEECCCCCCCCCCEEEECCCCCEEEEEECCC
KEYTYHKPANESGMPSPVITFKDVFDVMYDKKLSRQKKQADSYLLAEGLMDGKVVATHKV
CEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
TPTRRPSKLLLWADDEKVQMKADGSDIVTVIAAIADENGNIKRLNNYEVKFEIEGQGQLV
CCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHCCCCCEEEECCEEEEEEECCCCEEE
ANEETFTNPRPVLWGTAPVLVRSTTTPGKIKIRASVVWQGKHTPVPAELIIPTFPSEHTL
ECCCCCCCCCCEEEECCCEEEEECCCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCEE
VADKDELTQAQSASKDAGNKINAAPSDCEKRVLELQQELNRLKLKEVEKQQSDFE
EECHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSTSTWWKILLSGLLLAGTLVANADTYQPSYSTAGFYQLSNTGRTVYNMNPAWRFYKGN
CCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCEEEECCCCCEEEECCCCEEEEECC
IEGAEQPRFNDKDWNIVSLPDGIEYLPTEASGCINYQGEIWYRKHFTPKESWKGKQQFLH
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEECCCCEEEEECCCCHHHCCCHHHHEE
FEAIMGKSKIWVNGKLLKEHFGGFLPVIVDVTSSLKYGEDNVITVWADNSDDPSYPPGKP
HHHHCCCCEEEECCEEHHHHCCCCEEEEEEHHHCCCCCCCCEEEEEECCCCCCCCCCCCC
QDALDFAYFGGIYRDCWMIVHNKVFITDPNYENETAGGGLFVSFDKVSEQSAQLKLDAHI
CHHHHHHHHHHHHHHHEEEEECEEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEE
RNTSGKSFSGKIIYELYDRDDKRVLSKETGLSVSKDLAKQISCKVTVETPHLWSPDSPYL
ECCCCCCCCCEEEEEEECCCCHHHHHHHCCCCHHHHHHHCCEEEEEEECCCCCCCCCCEE
YKLHIYIKDKAGNTIDGYYRRIGIRSIEFKGKDGFWLNGKPYPYPLMGANRHQDFAVIGN
EEEEEEEECCCCCCHHHHHHHHCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEECC
ALPNSLHWRDAKKLRDAGMRVIRNAHYPQDPAFMDACDELGLFVIVNTPGWQFWNDQPIF
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCHH
AQRVYSDIRNMVRRDRNHPSVWMWEPILNETWYPADFAKNVVDILNEEYPYPYCYAGCDV
HHHHHHHHHHHHHHCCCCCCEEEECHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCE
TARGHEYFPIHFTHPMNGGGGAFNTENLDPKISYFTREWGDNVDDWNSHNSPSRVNRGWG
EECCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCC
EVPMLIQAQGYAKNDYQLTSYDGLYRTSRQHMGGCLWHSFDHQRGYHPDPFYGGIMDAFR
CCCEEEEECCCCCCCCEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHC
QPKLSYYMFCSQRPAEPNKELIADNGPMIYIANAMTPFSPKDVTIYSNCDEVRLTYCKGG
CCCCEEEEEECCCCCCCCHHEEECCCCEEEEECCCCCCCCCCEEEECCCCCEEEEEECCC
KEYTYHKPANESGMPSPVITFKDVFDVMYDKKLSRQKKQADSYLLAEGLMDGKVVATHKV
CEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
TPTRRPSKLLLWADDEKVQMKADGSDIVTVIAAIADENGNIKRLNNYEVKFEIEGQGQLV
CCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHCCCCCEEEECCEEEEEEECCCCEEE
ANEETFTNPRPVLWGTAPVLVRSTTTPGKIKIRASVVWQGKHTPVPAELIIPTFPSEHTL
ECCCCCCCCCCEEEECCCEEEEECCCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCEE
VADKDELTQAQSASKDAGNKINAAPSDCEKRVLELQQELNRLKLKEVEKQQSDFE
EECHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA