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The map label for this gene is metF [H]

Identifier: 29349229

GI number: 29349229

Start: 4973330

End: 4974283

Strand: Reverse

Name: metF [H]

Synonym: BT_3821

Alternate gene names: 29349229

Gene position: 4974283-4973330 (Counterclockwise)

Preceding gene: 29349230

Following gene: 29349228

Centisome position: 79.46

GC content: 46.23

Gene sequence:

>954_bases
ATGAGAGTAATTGATTTAATACATAACAGCGAAAAAACAGCTTTCTCTTTCGAAATCCTTCCTCCACTGAAAGGTACAGG
CATCGAAAAGCTGTATCAGACTGTCGATACCCTTCGGGAGTTTGATCCGAAATATATCAATATCACCACGCATCGCAGCG
AATACGTATATAAGGATCTGGGCAACGGACTTTTCCAGCGAAACCGGCTGAGAAGACGCCCGGGGACTGTTGCCGTAGCT
GCCGCTATCCAGAACAAGTATAATATAACGGTGGTTCCGCACATACTGTGCAGCGGCTTCACCCGTGAAGAAACCGAATA
TGTACTGCTCGACCTGCAATTCCTCAATATCACCGATCTGCTGGTACTTCGGGGAGATAAGGCCAAACATGAATCAGTAT
TCACTCCCGAGGGCGACGGATACCATCATGCTATCGAGTTGCAGGAGCAAATCAATAACTTCAATAAGGGAATCTTTGTA
GACGGTTCGGAGATGAAAGTAACCAACAGCCCGTTCTCCTACGGTGTGGCTTGCTATCCGGAGAAGCACGAGGAGGCTCC
CAACATCGAATCCGATCTCTTCTGGCTGAAAAAGAAAGTAGAAGCAGGTGCGGAATATGCTGTGACACAGTTATTCTACG
ACAATAAGAAATACTTCGAATTTGTAGAGCAAGCGAAGGCAGCAGGTATCAATGTACCTATCATTCCGGGCATCAAGCCA
TTCAAGAAGTTATCTCAACTCAGCATGGTGCCCAAGACATTCAAAGTAGACTTGCCGGAAGATTTGGTCAAGGAGGCTTT
GAAATGCAAGAACGACAAGGAAGCCGAACAGGTTGGTATCGAATGGTGTGTCGCCCAATGCAAAGAGTTAATGGCACATG
GAGTGCCAAGCATCCACTTCTACTCCATCGGAGCAGTGGACAGTATCAAAGAAGTGGCTAAAATCATTTATTGA

Upstream 100 bases:

>100_bases
ACTATTTTCAAACAAATCAAGAAGAAATCTTTCTTTTTTCGTATTTTTGTCCCAATATGCACTTTTAATTCAAGTAGACA
ACGAACATCAACGAAAAAGA

Downstream 100 bases:

>100_bases
TATGTTTTTCAGAGACGTAATCGGACAAGAAGAAATCAAGCAACGACTCATTCAGGAAGTGAGTGAAGGGCGCATACCGC
ATGCCCAACTGATCTGTGGC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRVIDLIHNSEKTAFSFEILPPLKGTGIEKLYQTVDTLREFDPKYINITTHRSEYVYKDLGNGLFQRNRLRRRPGTVAVA
AAIQNKYNITVVPHILCSGFTREETEYVLLDLQFLNITDLLVLRGDKAKHESVFTPEGDGYHHAIELQEQINNFNKGIFV
DGSEMKVTNSPFSYGVACYPEKHEEAPNIESDLFWLKKKVEAGAEYAVTQLFYDNKKYFEFVEQAKAAGINVPIIPGIKP
FKKLSQLSMVPKTFKVDLPEDLVKEALKCKNDKEAEQVGIEWCVAQCKELMAHGVPSIHFYSIGAVDSIKEVAKIIY

Sequences:

>Translated_317_residues
MRVIDLIHNSEKTAFSFEILPPLKGTGIEKLYQTVDTLREFDPKYINITTHRSEYVYKDLGNGLFQRNRLRRRPGTVAVA
AAIQNKYNITVVPHILCSGFTREETEYVLLDLQFLNITDLLVLRGDKAKHESVFTPEGDGYHHAIELQEQINNFNKGIFV
DGSEMKVTNSPFSYGVACYPEKHEEAPNIESDLFWLKKKVEAGAEYAVTQLFYDNKKYFEFVEQAKAAGINVPIIPGIKP
FKKLSQLSMVPKTFKVDLPEDLVKEALKCKNDKEAEQVGIEWCVAQCKELMAHGVPSIHFYSIGAVDSIKEVAKIIY
>Mature_317_residues
MRVIDLIHNSEKTAFSFEILPPLKGTGIEKLYQTVDTLREFDPKYINITTHRSEYVYKDLGNGLFQRNRLRRRPGTVAVA
AAIQNKYNITVVPHILCSGFTREETEYVLLDLQFLNITDLLVLRGDKAKHESVFTPEGDGYHHAIELQEQINNFNKGIFV
DGSEMKVTNSPFSYGVACYPEKHEEAPNIESDLFWLKKKVEAGAEYAVTQLFYDNKKYFEFVEQAKAAGINVPIIPGIKP
FKKLSQLSMVPKTFKVDLPEDLVKEALKCKNDKEAEQVGIEWCVAQCKELMAHGVPSIHFYSIGAVDSIKEVAKIIY

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=303, Percent_Identity=30.6930693069307, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1790377, Length=294, Percent_Identity=27.2108843537415, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25147441, Length=313, Percent_Identity=26.8370607028754, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25147436, Length=317, Percent_Identity=25.5520504731861, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321313, Length=299, Percent_Identity=26.7558528428094, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6325234, Length=334, Percent_Identity=24.8502994011976, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24662658, Length=243, Percent_Identity=24.6913580246914, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 36042; Mature: 36042

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVIDLIHNSEKTAFSFEILPPLKGTGIEKLYQTVDTLREFDPKYINITTHRSEYVYKDL
CEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
GNGLFQRNRLRRRPGTVAVAAAIQNKYNITVVPHILCSGFTREETEYVLLDLQFLNITDL
CCHHHHHHHHHCCCCCEEEEEECCCCCEEEEEHHHHHCCCCCCCCEEEEEEEEECCCEEE
LVLRGDKAKHESVFTPEGDGYHHAIELQEQINNFNKGIFVDGSEMKVTNSPFSYGVACYP
EEECCCCCCCCCCCCCCCCCCCHHEEHHHHHCCCCCCEEEECCCEEEECCCCCCCEEECC
EKHEEAPNIESDLFWLKKKVEAGAEYAVTQLFYDNKKYFEFVEQAKAAGINVPIIPGIKP
HHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHH
FKKLSQLSMVPKTFKVDLPEDLVKEALKCKNDKEAEQVGIEWCVAQCKELMAHGVPSIHF
HHHHHHHHCCCCCEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
YSIGAVDSIKEVAKIIY
EECCCHHHHHHHHHHCC
>Mature Secondary Structure
MRVIDLIHNSEKTAFSFEILPPLKGTGIEKLYQTVDTLREFDPKYINITTHRSEYVYKDL
CEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
GNGLFQRNRLRRRPGTVAVAAAIQNKYNITVVPHILCSGFTREETEYVLLDLQFLNITDL
CCHHHHHHHHHCCCCCEEEEEECCCCCEEEEEHHHHHCCCCCCCCEEEEEEEEECCCEEE
LVLRGDKAKHESVFTPEGDGYHHAIELQEQINNFNKGIFVDGSEMKVTNSPFSYGVACYP
EEECCCCCCCCCCCCCCCCCCCHHEEHHHHHCCCCCCEEEECCCEEEECCCCCCCEEECC
EKHEEAPNIESDLFWLKKKVEAGAEYAVTQLFYDNKKYFEFVEQAKAAGINVPIIPGIKP
HHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHH
FKKLSQLSMVPKTFKVDLPEDLVKEALKCKNDKEAEQVGIEWCVAQCKELMAHGVPSIHF
HHHHHHHHCCCCCEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
YSIGAVDSIKEVAKIIY
EECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]