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The map label for this gene is holB [H]

Identifier: 29349228

GI number: 29349228

Start: 4972204

End: 4973328

Strand: Reverse

Name: holB [H]

Synonym: BT_3820

Alternate gene names: 29349228

Gene position: 4973328-4972204 (Counterclockwise)

Preceding gene: 29349229

Following gene: 29349227

Centisome position: 79.44

GC content: 46.84

Gene sequence:

>1125_bases
ATGTTTTTCAGAGACGTAATCGGACAAGAAGAAATCAAGCAACGACTCATTCAGGAAGTGAGTGAAGGGCGCATACCGCA
TGCCCAACTGATCTGTGGCCCCGAAGGAGTCGGTAAGATGCCGCTGGCTATCGCTTATGCCCGCTACATCAGTTGCACCA
ACCGGGGTGAGACGGATGCCTGCGGAGTATGTCCGTCGTGTGTGAAATTCAATAAACTGGTACATCCGGACGTACATTTC
GTATTCCCCATCGTGAAAAGTTCTAAAGGAAAACGGGAAGTGTGCGACGACTATATCGCCGACTGGAGACCATTCGTTCT
GAAGAATCCGTACTTCAACCTGAATCACTGGCTGAGAGAAATGGATGCCGAGAATGCACAGGCCATCATCTACGCAAAAG
AGAGCGATGAAATCCTGAAGAAACTCAGCCTCAAATCGAGCGAAGGAGGATTCAAGATCACGATTCTCTGGTTGCCCGAA
AAGATGTATCCGGTATGTGCCAACAAATTATTGAAATTGCTCGAAGAGCCACCTGAGAAAACTATCTTCCTGCTCGTATC
GGAAGCTCCGGAAATGATTCTGCCTACTATTCTGAGCCGTACTCAACGCATGAACGTGCGAAAAATAGACGAACCGGCCA
TCGACCGCGTATTGCAGTCCAAATACGGTATCCAGCCTGCCGACAGCCTTTCCATTGCCCATCTGGCAAACGGGAACTTT
ATCAAAGCACTGGAAACTATTCATCTGAATGAAGAGAATCAGCTCTTCTTCGACTTATTTGTCAGCCTGATGCGATTGTC
CTATCAACGGAAAATCAGGGAAATGAAGCTCTGGAGCGAACAGGTTGCCGGCATGGGACGTGAACGCCAGAAGAACTTTC
TGGAATACTGCCAGCGCATGATCCGCGAAAACTTTATATTCAATCTCCATCAGCGTGATCTGACATACATGACAATCAAC
GAACAGAATTTCGCCTCTCGCTTTGCTCCTTTCGTCAACGAAAGGAACGTAATAGGCATCATGGATGAACTAAGCGAAGC
GCAACTGCATATAGAACAAAATGTAAATGCCAAGATGGTATTTTTCGACTTCTCTCTGAAGATGATCGTACTGTTGAAGC
AATAA

Upstream 100 bases:

>100_bases
CAATGCAAAGAGTTAATGGCACATGGAGTGCCAAGCATCCACTTCTACTCCATCGGAGCAGTGGACAGTATCAAAGAAGT
GGCTAAAATCATTTATTGAT

Downstream 100 bases:

>100_bases
TAAATATATAAACTATGGAATATAAGCTTCATAATGGGAGCGGCGGTCTTTGCTGCAAAGGATGCTCCCGACAAGATAAG
AAACTGAACACTTACGATTG

Product: putative DNA polymerase III, delta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MFFRDVIGQEEIKQRLIQEVSEGRIPHAQLICGPEGVGKMPLAIAYARYISCTNRGETDACGVCPSCVKFNKLVHPDVHF
VFPIVKSSKGKREVCDDYIADWRPFVLKNPYFNLNHWLREMDAENAQAIIYAKESDEILKKLSLKSSEGGFKITILWLPE
KMYPVCANKLLKLLEEPPEKTIFLLVSEAPEMILPTILSRTQRMNVRKIDEPAIDRVLQSKYGIQPADSLSIAHLANGNF
IKALETIHLNEENQLFFDLFVSLMRLSYQRKIREMKLWSEQVAGMGRERQKNFLEYCQRMIRENFIFNLHQRDLTYMTIN
EQNFASRFAPFVNERNVIGIMDELSEAQLHIEQNVNAKMVFFDFSLKMIVLLKQ

Sequences:

>Translated_374_residues
MFFRDVIGQEEIKQRLIQEVSEGRIPHAQLICGPEGVGKMPLAIAYARYISCTNRGETDACGVCPSCVKFNKLVHPDVHF
VFPIVKSSKGKREVCDDYIADWRPFVLKNPYFNLNHWLREMDAENAQAIIYAKESDEILKKLSLKSSEGGFKITILWLPE
KMYPVCANKLLKLLEEPPEKTIFLLVSEAPEMILPTILSRTQRMNVRKIDEPAIDRVLQSKYGIQPADSLSIAHLANGNF
IKALETIHLNEENQLFFDLFVSLMRLSYQRKIREMKLWSEQVAGMGRERQKNFLEYCQRMIRENFIFNLHQRDLTYMTIN
EQNFASRFAPFVNERNVIGIMDELSEAQLHIEQNVNAKMVFFDFSLKMIVLLKQ
>Mature_374_residues
MFFRDVIGQEEIKQRLIQEVSEGRIPHAQLICGPEGVGKMPLAIAYARYISCTNRGETDACGVCPSCVKFNKLVHPDVHF
VFPIVKSSKGKREVCDDYIADWRPFVLKNPYFNLNHWLREMDAENAQAIIYAKESDEILKKLSLKSSEGGFKITILWLPE
KMYPVCANKLLKLLEEPPEKTIFLLVSEAPEMILPTILSRTQRMNVRKIDEPAIDRVLQSKYGIQPADSLSIAHLANGNF
IKALETIHLNEENQLFFDLFVSLMRLSYQRKIREMKLWSEQVAGMGRERQKNFLEYCQRMIRENFIFNLHQRDLTYMTIN
EQNFASRFAPFVNERNVIGIMDELSEAQLHIEQNVNAKMVFFDFSLKMIVLLKQ

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 43432; Mature: 43432

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFRDVIGQEEIKQRLIQEVSEGRIPHAQLICGPEGVGKMPLAIAYARYISCTNRGETDA
CCCHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCC
CGVCPSCVKFNKLVHPDVHFVFPIVKSSKGKREVCDDYIADWRPFVLKNPYFNLNHWLRE
CCCCHHHHHHHHCCCCCHHEEEEHHHCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHH
MDAENAQAIIYAKESDEILKKLSLKSSEGGFKITILWLPEKMYPVCANKLLKLLEEPPEK
HCCCCCEEEEEECCHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
TIFLLVSEAPEMILPTILSRTQRMNVRKIDEPAIDRVLQSKYGIQPADSLSIAHLANGNF
EEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCC
IKALETIHLNEENQLFFDLFVSLMRLSYQRKIREMKLWSEQVAGMGRERQKNFLEYCQRM
EEHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IRENFIFNLHQRDLTYMTINEQNFASRFAPFVNERNVIGIMDELSEAQLHIEQNVNAKMV
HHHHHEEEEECCCEEEEEECCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCEEEE
FFDFSLKMIVLLKQ
EEECCEEEEEEECC
>Mature Secondary Structure
MFFRDVIGQEEIKQRLIQEVSEGRIPHAQLICGPEGVGKMPLAIAYARYISCTNRGETDA
CCCHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCC
CGVCPSCVKFNKLVHPDVHFVFPIVKSSKGKREVCDDYIADWRPFVLKNPYFNLNHWLRE
CCCCHHHHHHHHCCCCCHHEEEEHHHCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHH
MDAENAQAIIYAKESDEILKKLSLKSSEGGFKITILWLPEKMYPVCANKLLKLLEEPPEK
HCCCCCEEEEEECCHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
TIFLLVSEAPEMILPTILSRTQRMNVRKIDEPAIDRVLQSKYGIQPADSLSIAHLANGNF
EEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCC
IKALETIHLNEENQLFFDLFVSLMRLSYQRKIREMKLWSEQVAGMGRERQKNFLEYCQRM
EEHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IRENFIFNLHQRDLTYMTINEQNFASRFAPFVNERNVIGIMDELSEAQLHIEQNVNAKMV
HHHHHEEEEECCCEEEEEECCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCEEEE
FFDFSLKMIVLLKQ
EEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]