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The map label for this gene is rlmL [H]

Identifier: 29348664

GI number: 29348664

Start: 4155921

End: 4157390

Strand: Reverse

Name: rlmL [H]

Synonym: BT_3255

Alternate gene names: 29348664

Gene position: 4157390-4155921 (Counterclockwise)

Preceding gene: 29348665

Following gene: 29348663

Centisome position: 66.41

GC content: 47.41

Gene sequence:

>1470_bases
ATGAGCGAACAATTCGAGATGATAGCCAAGACCTTCCAGGGACTGGAAGAGATACTTGCTGAAGAACTTACAACATTGGG
AGCCAACGATATCCAGATAGGACGTCGCATGGTTTCATTTACAGGAGACAAGCGTATGATGTACAAGGCAAATTTCTGCC
TCCGTACTGCTATCCGCATTCTAAAACCGATCAAGAATTTTACGGCAAAGGATGCTGATGAAGTTTACAACCAGATACAG
GCCATTCCATGGGAAGAGTATCTTGATGTAAACAAGACGTTTGCGATAGACGCGGTTGTATTCAGCGAAGAATTCCGCCA
TTCAAAGTTCGTCTCATATAAGGTGAAGGACGCCATCGTGGACTATTTCCGTAAAAAGACAGGCAAACGCCCGTCAGTAC
GCATCAATAATCCGGATGTGCTGCTCAATATTCACATTGCACAAACGACTTGTACGTTGTCTCTGGACTCTTCCGGAGAG
TCACTTCATCGTCGCGGATATCGCCAGGAACAGGTAGATGCTCCGTTGAACGAAGTATTGGCTGCCGGTATGCTTCTGAT
GACAGGATGGCGCGGTGAATGTGACCTGATTGACCCGATGTGTGGTTCGGGAACGATTCCTATAGAAGCTGCGCTGATCG
CAAGAAACATCGCTCCGGGAGTATTCCGCAAAGGATTCGCATTCGAGAAATGGGTGGACTTCGATTCGGAGATGTTCGAT
GAGATTTACAATGACGATAGTCAGGAGCGCGAGTTTACTCACAAGATATACGGATACGACAACAATCCGAAAGCAAATGA
AATCGCAACCCATAATATAAAGGCAGCGGGTGTATCCAAAGATGTGACGCTGAAACTGCAACCGTTCCAACAATTCGAAC
AGCCGCAGGAGAAGTCCATCATTGTGATGAATCCTCCTTATGGCGAGCGTATCTCCACCAATGACCTGCTCGGACTGTAC
CAGATGATTGGCGAACGTCTGAAGCACGCATTTGTAGGAAACGAGGCTTGGGTACTCTCTTATAGAGAAGAATGTTTCGA
TCAGATCGGGCTGAAACCAAGCAAGAAAGTGCCGTTGTTCAATGGTGCACTGGAATGCGAGTTCCGCAAATACGAGATTT
TCGACGGGAAATATAAGGAGTTCAAGAGTCAGGAGGGAGAAGGAGAAGAGAAGAAAGAGACTGAAGGAAACTATGATACT
TCCAGACCCAGAGAAAGAAAGGAATTCAAACCGCGCGGGGAAGGAGAATTTAAACCCAGACGGGAAGGCGAATTCAAACC
TCGTCGCGAAGGGGAGTTCAAACCTCGTAAAGAAGGAGACTTCAAGCCGAGACGGGAAGAAGGAAGCTTTACCCGGGATG
ACAGGGACAGAAAGCCTAGAGGCGAGTTCCGAGGGGAAAGAGATTCGAGAGCTCCGAGAGAATTCAGAGGAAACCGTGAG
CCTAGGATACCAAAGAAGGAAGAAGAGTAA

Upstream 100 bases:

>100_bases
GACGCAACCGTAGGAGGAAATATCTGGGTAACGGAAAACATTCCTGCCGGAGCAAAGATTGTACAAACAAAAGCAAAGAA
ATAAAATCAATAAACTATAT

Downstream 100 bases:

>100_bases
TTTCATAGATAGTTAATGGCATCTCACTAAAAAATGCCATTAACTTATTGATAGACAATATAAGGTCGCATCTTGCGATC
TTATCATTTGATGAGTAAGA

Product: putative N6-adenine-specific DNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSEQFEMIAKTFQGLEEILAEELTTLGANDIQIGRRMVSFTGDKRMMYKANFCLRTAIRILKPIKNFTAKDADEVYNQIQ
AIPWEEYLDVNKTFAIDAVVFSEEFRHSKFVSYKVKDAIVDYFRKKTGKRPSVRINNPDVLLNIHIAQTTCTLSLDSSGE
SLHRRGYRQEQVDAPLNEVLAAGMLLMTGWRGECDLIDPMCGSGTIPIEAALIARNIAPGVFRKGFAFEKWVDFDSEMFD
EIYNDDSQEREFTHKIYGYDNNPKANEIATHNIKAAGVSKDVTLKLQPFQQFEQPQEKSIIVMNPPYGERISTNDLLGLY
QMIGERLKHAFVGNEAWVLSYREECFDQIGLKPSKKVPLFNGALECEFRKYEIFDGKYKEFKSQEGEGEEKKETEGNYDT
SRPRERKEFKPRGEGEFKPRREGEFKPRREGEFKPRKEGDFKPRREEGSFTRDDRDRKPRGEFRGERDSRAPREFRGNRE
PRIPKKEEE

Sequences:

>Translated_489_residues
MSEQFEMIAKTFQGLEEILAEELTTLGANDIQIGRRMVSFTGDKRMMYKANFCLRTAIRILKPIKNFTAKDADEVYNQIQ
AIPWEEYLDVNKTFAIDAVVFSEEFRHSKFVSYKVKDAIVDYFRKKTGKRPSVRINNPDVLLNIHIAQTTCTLSLDSSGE
SLHRRGYRQEQVDAPLNEVLAAGMLLMTGWRGECDLIDPMCGSGTIPIEAALIARNIAPGVFRKGFAFEKWVDFDSEMFD
EIYNDDSQEREFTHKIYGYDNNPKANEIATHNIKAAGVSKDVTLKLQPFQQFEQPQEKSIIVMNPPYGERISTNDLLGLY
QMIGERLKHAFVGNEAWVLSYREECFDQIGLKPSKKVPLFNGALECEFRKYEIFDGKYKEFKSQEGEGEEKKETEGNYDT
SRPRERKEFKPRGEGEFKPRREGEFKPRREGEFKPRKEGDFKPRREEGSFTRDDRDRKPRGEFRGERDSRAPREFRGNRE
PRIPKKEEE
>Mature_488_residues
SEQFEMIAKTFQGLEEILAEELTTLGANDIQIGRRMVSFTGDKRMMYKANFCLRTAIRILKPIKNFTAKDADEVYNQIQA
IPWEEYLDVNKTFAIDAVVFSEEFRHSKFVSYKVKDAIVDYFRKKTGKRPSVRINNPDVLLNIHIAQTTCTLSLDSSGES
LHRRGYRQEQVDAPLNEVLAAGMLLMTGWRGECDLIDPMCGSGTIPIEAALIARNIAPGVFRKGFAFEKWVDFDSEMFDE
IYNDDSQEREFTHKIYGYDNNPKANEIATHNIKAAGVSKDVTLKLQPFQQFEQPQEKSIIVMNPPYGERISTNDLLGLYQ
MIGERLKHAFVGNEAWVLSYREECFDQIGLKPSKKVPLFNGALECEFRKYEIFDGKYKEFKSQEGEGEEKKETEGNYDTS
RPRERKEFKPRGEGEFKPRREGEFKPRREGEFKPRKEGDFKPRREEGSFTRDDRDRKPRGEFRGERDSRAPREFRGNREP
RIPKKEEE

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=378, Percent_Identity=33.5978835978836, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 56791; Mature: 56660

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQFEMIAKTFQGLEEILAEELTTLGANDIQIGRRMVSFTGDKRMMYKANFCLRTAIRI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHH
LKPIKNFTAKDADEVYNQIQAIPWEEYLDVNKTFAIDAVVFSEEFRHSKFVSYKVKDAIV
HHHHHHHCCCCHHHHHHHHHCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
DYFRKKTGKRPSVRINNPDVLLNIHIAQTTCTLSLDSSGESLHRRGYRQEQVDAPLNEVL
HHHHHHCCCCCCEEECCCCEEEEEEEEEEEEEEEECCCCHHHHHCCCCHHHHCCCHHHHH
AAGMLLMTGWRGECDLIDPMCGSGTIPIEAALIARNIAPGVFRKGFAFEKWVDFDSEMFD
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCCHHHHHCCCHHHHH
EIYNDDSQEREFTHKIYGYDNNPKANEIATHNIKAAGVSKDVTLKLQPFQQFEQPQEKSI
HHHCCCCHHHHHHHHHCCCCCCCCCCCHHHCCEEECCCCCCEEEEECCHHHHCCCCCCEE
IVMNPPYGERISTNDLLGLYQMIGERLKHAFVGNEAWVLSYREECFDQIGLKPSKKVPLF
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCCCC
NGALECEFRKYEIFDGKYKEFKSQEGEGEEKKETEGNYDTSRPRERKEFKPRGEGEFKPR
CCCEEEEEEEEEECCCHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHCCCCCCCCCCCC
REGEFKPRREGEFKPRKEGDFKPRREEGSFTRDDRDRKPRGEFRGERDSRAPREFRGNRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
PRIPKKEEE
CCCCCCCCC
>Mature Secondary Structure 
SEQFEMIAKTFQGLEEILAEELTTLGANDIQIGRRMVSFTGDKRMMYKANFCLRTAIRI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHH
LKPIKNFTAKDADEVYNQIQAIPWEEYLDVNKTFAIDAVVFSEEFRHSKFVSYKVKDAIV
HHHHHHHCCCCHHHHHHHHHCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
DYFRKKTGKRPSVRINNPDVLLNIHIAQTTCTLSLDSSGESLHRRGYRQEQVDAPLNEVL
HHHHHHCCCCCCEEECCCCEEEEEEEEEEEEEEEECCCCHHHHHCCCCHHHHCCCHHHHH
AAGMLLMTGWRGECDLIDPMCGSGTIPIEAALIARNIAPGVFRKGFAFEKWVDFDSEMFD
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCCHHHHHCCCHHHHH
EIYNDDSQEREFTHKIYGYDNNPKANEIATHNIKAAGVSKDVTLKLQPFQQFEQPQEKSI
HHHCCCCHHHHHHHHHCCCCCCCCCCCHHHCCEEECCCCCCEEEEECCHHHHCCCCCCEE
IVMNPPYGERISTNDLLGLYQMIGERLKHAFVGNEAWVLSYREECFDQIGLKPSKKVPLF
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCCCC
NGALECEFRKYEIFDGKYKEFKSQEGEGEEKKETEGNYDTSRPRERKEFKPRGEGEFKPR
CCCEEEEEEEEEECCCHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHCCCCCCCCCCCC
REGEFKPRREGEFKPRKEGDFKPRREEGSFTRDDRDRKPRGEFRGERDSRAPREFRGNRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
PRIPKKEEE
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA