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The map label for this gene is purD [H]

Identifier: 29348662

GI number: 29348662

Start: 4152166

End: 4153440

Strand: Reverse

Name: purD [H]

Synonym: BT_3253

Alternate gene names: 29348662

Gene position: 4153440-4152166 (Counterclockwise)

Preceding gene: 29348663

Following gene: 29348661

Centisome position: 66.35

GC content: 51.76

Gene sequence:

>1275_bases
ATGAAGATATTATTACTAGGCTCGGGCGGCCGCGAACATGCACTGGCATGGAAAATTGCCCAAAGTCCGAAAGTTGAAAA
GCTGTACATCGCTCCGGGAAACGCCGGAACAAACGCCGTAGGCGAGAATGTCAACATCAAAGCTACCGACTTTGAAGCGA
TCAGCGCTTTCGCTCTGAAAGAAAACATCGAAATGGTAGTAGTAGGTCCCGAAGACCCGTTGGTAGAAGGCATCTACGAT
TACTTCCAGAATCGTCCCGAACTGAAACATATCGCCGTTATCGGTCCGTCCGCCAACGGAGCACAGCTGGAGGGCAGCAA
GGAGTTTGCCAAAGGCTTCATGATGCGTCATCAAATCCCGACAGCCCGGTATAAAAGTATCACAGCAGAAAATCTCGAAG
AAGGTCTGGCTTTCCTCGAAACGCTGGAAGCCCCATACGTGCTGAAAGCCGACGGACTCTGTGCCGGAAAAGGCGTTCTG
ATCCTCCCCACACTGGACGAGGCAAAGAAAGAACTCAAGGAAATGCTTGGCGGTATGTTCGGCTCCGCATCGGCAACAGT
CGTTATTGAAGAGTTCCTGAGCGGTATCGAATGTTCCGTATTCGTGCTTACCGACGGCGAACACTACAAAGTCCTCCCCG
TAGCGAAAGACTACAAGCGTATCGGTGAAGGCGACAAAGGACTCAACACAGGCGGTATGGGCAGCGTGACTCCGGTTCCG
TTTGCCGATGAAGAATACATGGAAAAAGTACGTACCCGCATCATCGAACCTACCATCAACGGACTGAAAGAAGAAAACAT
CGTCTACAAAGGCTTCATCTTCCTCGGACTGATCCGGGTAAAAGGCGAACCGATGGTGATTGAATATAACGTCCGCATGG
GCGACCCGGAAACGGAAAGCGTGATGCTCCGCATCCAAAGCGACCTCGTGGAATTGCTGGAAGGCACAGCCGCAGGCAAT
CTCGATGAAAAGACACTGGTCATGGACCCGCGCTCGGCAGGTTGTGTGATCCTTGTGAGCGGCGGTTATCCTGAAGCATA
CGAAAAAGGCTTCCCTATCAGCGGCCTGGAACAGGCCGCCGCTACCGAAAGCATCATTTTCCATGCCGGAACAGCCATGA
AGGACGGACAAATCGTAACGAACGGGGGACGTGTGATCGCTGTCTGCTCTTATGGCGCAACGAAGGAAGAAGCGCTGGCA
CAGAGCTATAAAGTGGCAGATATGATCGACTTCGACAAGAAATATTTCCGTCGCGATATCGGATTCGACTTATAA

Upstream 100 bases:

>100_bases
ACCCGATACTTTGAGCAGAACCTATAGCAATCTATAGTTGACAGCTCACAGCATCATACCGAAAAACTCATAACTGAAAA
CTCATAACTTAATACTCATT

Downstream 100 bases:

>100_bases
TGAGAAACAAGAGACTACAAGGTCTGGTCACAGCAGGGCGGTGGACACTCCCTGCTGCGATTTTCATCTGCACCCTTTGT
TGGGTGCTGACCTCTGTCCT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKILLLGSGGREHALAWKIAQSPKVEKLYIAPGNAGTNAVGENVNIKATDFEAISAFALKENIEMVVVGPEDPLVEGIYD
YFQNRPELKHIAVIGPSANGAQLEGSKEFAKGFMMRHQIPTARYKSITAENLEEGLAFLETLEAPYVLKADGLCAGKGVL
ILPTLDEAKKELKEMLGGMFGSASATVVIEEFLSGIECSVFVLTDGEHYKVLPVAKDYKRIGEGDKGLNTGGMGSVTPVP
FADEEYMEKVRTRIIEPTINGLKEENIVYKGFIFLGLIRVKGEPMVIEYNVRMGDPETESVMLRIQSDLVELLEGTAAGN
LDEKTLVMDPRSAGCVILVSGGYPEAYEKGFPISGLEQAAATESIIFHAGTAMKDGQIVTNGGRVIAVCSYGATKEEALA
QSYKVADMIDFDKKYFRRDIGFDL

Sequences:

>Translated_424_residues
MKILLLGSGGREHALAWKIAQSPKVEKLYIAPGNAGTNAVGENVNIKATDFEAISAFALKENIEMVVVGPEDPLVEGIYD
YFQNRPELKHIAVIGPSANGAQLEGSKEFAKGFMMRHQIPTARYKSITAENLEEGLAFLETLEAPYVLKADGLCAGKGVL
ILPTLDEAKKELKEMLGGMFGSASATVVIEEFLSGIECSVFVLTDGEHYKVLPVAKDYKRIGEGDKGLNTGGMGSVTPVP
FADEEYMEKVRTRIIEPTINGLKEENIVYKGFIFLGLIRVKGEPMVIEYNVRMGDPETESVMLRIQSDLVELLEGTAAGN
LDEKTLVMDPRSAGCVILVSGGYPEAYEKGFPISGLEQAAATESIIFHAGTAMKDGQIVTNGGRVIAVCSYGATKEEALA
QSYKVADMIDFDKKYFRRDIGFDL
>Mature_424_residues
MKILLLGSGGREHALAWKIAQSPKVEKLYIAPGNAGTNAVGENVNIKATDFEAISAFALKENIEMVVVGPEDPLVEGIYD
YFQNRPELKHIAVIGPSANGAQLEGSKEFAKGFMMRHQIPTARYKSITAENLEEGLAFLETLEAPYVLKADGLCAGKGVL
ILPTLDEAKKELKEMLGGMFGSASATVVIEEFLSGIECSVFVLTDGEHYKVLPVAKDYKRIGEGDKGLNTGGMGSVTPVP
FADEEYMEKVRTRIIEPTINGLKEENIVYKGFIFLGLIRVKGEPMVIEYNVRMGDPETESVMLRIQSDLVELLEGTAAGN
LDEKTLVMDPRSAGCVILVSGGYPEAYEKGFPISGLEQAAATESIIFHAGTAMKDGQIVTNGGRVIAVCSYGATKEEALA
QSYKVADMIDFDKKYFRRDIGFDL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=427, Percent_Identity=41.4519906323185, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI209869995, Length=427, Percent_Identity=41.4519906323185, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI209869993, Length=427, Percent_Identity=41.4519906323185, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI28416899, Length=427, Percent_Identity=41.4519906323185, Blast_Score=322, Evalue=3e-88,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=46.8384074941452, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=41.6470588235294, Blast_Score=322, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6321203, Length=442, Percent_Identity=40.7239819004525, Blast_Score=317, Evalue=3e-87,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=41.8981481481481, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=41.8981481481481, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46012; Mature: 46012

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLLGSGGREHALAWKIAQSPKVEKLYIAPGNAGTNAVGENVNIKATDFEAISAFALK
CEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEECCHHHHHHHEEC
ENIEMVVVGPEDPLVEGIYDYFQNRPELKHIAVIGPSANGAQLEGSKEFAKGFMMRHQIP
CCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
TARYKSITAENLEEGLAFLETLEAPYVLKADGLCAGKGVLILPTLDEAKKELKEMLGGMF
CHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCEECCCCEEEEECHHHHHHHHHHHHHHHC
GSASATVVIEEFLSGIECSVFVLTDGEHYKVLPVAKDYKRIGEGDKGLNTGGMGSVTPVP
CCCCHHEEHHHHHCCCCEEEEEEECCCCEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCC
FADEEYMEKVRTRIIEPTINGLKEENIVYKGFIFLGLIRVKGEPMVIEYNVRMGDPETES
CCCHHHHHHHHHHHHCHHHCCCCCCCEEEEEEEEEEEEEECCCCEEEEEEEECCCCCCHH
VMLRIQSDLVELLEGTAAGNLDEKTLVMDPRSAGCVILVSGGYPEAYEKGFPISGLEQAA
HHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEEECCCCHHHHCCCCCCCHHHHH
ATESIIFHAGTAMKDGQIVTNGGRVIAVCSYGATKEEALAQSYKVADMIDFDKKYFRRDI
HHCEEEEECCCCCCCCEEEECCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHC
GFDL
CCCC
>Mature Secondary Structure
MKILLLGSGGREHALAWKIAQSPKVEKLYIAPGNAGTNAVGENVNIKATDFEAISAFALK
CEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEECCHHHHHHHEEC
ENIEMVVVGPEDPLVEGIYDYFQNRPELKHIAVIGPSANGAQLEGSKEFAKGFMMRHQIP
CCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
TARYKSITAENLEEGLAFLETLEAPYVLKADGLCAGKGVLILPTLDEAKKELKEMLGGMF
CHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCEECCCCEEEEECHHHHHHHHHHHHHHHC
GSASATVVIEEFLSGIECSVFVLTDGEHYKVLPVAKDYKRIGEGDKGLNTGGMGSVTPVP
CCCCHHEEHHHHHCCCCEEEEEEECCCCEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCC
FADEEYMEKVRTRIIEPTINGLKEENIVYKGFIFLGLIRVKGEPMVIEYNVRMGDPETES
CCCHHHHHHHHHHHHCHHHCCCCCCCEEEEEEEEEEEEEECCCCEEEEEEEECCCCCCHH
VMLRIQSDLVELLEGTAAGNLDEKTLVMDPRSAGCVILVSGGYPEAYEKGFPISGLEQAA
HHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEEECCCCHHHHCCCCCCCHHHHH
ATESIIFHAGTAMKDGQIVTNGGRVIAVCSYGATKEEALAQSYKVADMIDFDKKYFRRDI
HHCEEEEECCCCCCCCEEEECCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHC
GFDL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]