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The map label for this gene is lacZ [H]

Identifier: 29348501

GI number: 29348501

Start: 3925763

End: 3927967

Strand: Reverse

Name: lacZ [H]

Synonym: BT_3092

Alternate gene names: 29348501

Gene position: 3927967-3925763 (Counterclockwise)

Preceding gene: 29348502

Following gene: 29348500

Centisome position: 62.74

GC content: 43.72

Gene sequence:

>2205_bases
ATGAACAAGAAACTACTAATTGCAGTATTTGTAAGTGCTATGGCACATACGTTCGCTTGCGCAGCAAGCCCGAAAGAAAA
TAGAATGAAAACAGTTTGGGCTGATAAAGTTACGTCGGAAAATGTATGGCAGAGTTATCCGCGTCCTCAGTTACAACGTT
CGCAATGGATGAATCTGAACGGGTTATGGCAGTATGCGGTTACTACTCAGGATACACCTAAAAAAGAAGTGAAGTTCGAG
GGACAGATTCTCGTTCCGTTTGCTATCGAATCTTCCCTTTCGGGGGTGCAGCGTTCTTTTCTGCCTACCGAGAAATTGTG
GTATCAGCGTAACTTTACCCTTGATGACGCATGGAAAGGAAAAACAGTGATTTTGCATTTCGGAGCTGTAGATTATGAAT
GTCAGGTATGGGTAAACAACAAATTGGCGGGCATTCATAAAGGGGGGAATAATCCTTTCTCTTTCGATGTAACCAAATTG
CTGAGAAAAGGTGGTTCGCAACTTGTTGAGGTTGCTGTTATCGACCCTACGGATACGGAGTCAATTTCCCGTGGCAAGCA
ACAGTTGGAGCCGAAAGGTATCTGGTATTCTCCGGTATCCGGTATTTGGCAGACTGTGTGGTTGGAGGCTGTAAATTCAA
CCCATATTCTTCAGGTTCTTCCTACAGCTGATATTCATAAAAAGACGGTTAGTCTGGACATCAGTGTAGCTAAAGCCAAA
GGAGGAGAAAAAGTAAAAGTGGAATTACTGGACGAAGGTAAGGTAGTGGGTACGACAGAGCAAAAATTAGCCTCAAAGAT
AGAAATGGAAGTTCCGGAGGCTGTTCTTTGGAGTCCGTCTTCTCCGAAACTTTACCATCTGAATATTGAGTTGTTAAGTC
AAGGACAAGTAATAGATCAGGTAAAAAGCTATTTTGCGATGCGTGAGGTCAGTATCCGCAAAGATGAATGTGGTTACCAG
CGTATTTGTCTGAATGGTGATCCAATATTCCAGTATGGAACACTGGATCAAGGCTGGTGGCCGGATGGTTTGCTGACACC
TCCTTCCGAAGAAGCAATGCTTTGGGATATGGTTCAGCTCAAAAATATGGGATTTAATACCATACGCAAACACATTAAAG
TAGAACCGGAGCAATATTATTATTATGCCGATTCGTTGGGTTTGATGATGTGGCAGGATATGGTATCCGGCTTCTCTACC
GAAAGAAAAGACGAAGAACATATTAAGCCCAATGCGCAAACGGACTGGAATGCACCCGCATCACATACTAACCAATGGCA
ACAGGAGATGTTCGAGATGATTGACCGTCTGCGTTTTTATTCCTGTATCACAACGTGGGTAATCTTCAATGAAGGATGGG
GACAGCATAATACGGTAGAAATAGTGGAAAAGGTAATGGAATACGATAAAAGCCGTATCATTGACGGAGTAACCGGCTGG
ACAGACAGAGGAGTAGGCCATATGTATGATGTACACAATTATCCGTCTGCTTCCATGATTTTGCCCGCTTGTAATGGCAA
TCGGATTTCTGTCTTAGGAGAATTCGGAGGGTATGGATGGGCCATCAAAGAACATCTGTGGAATCCCGGTATGCGGAACT
GGGGATATAAAAATATTGATGGAGCAATGGCGCTGATGGATAACTATGGTCGTCTGGTCTATGATTTGGAGACATTGATA
GCACAGGGGCTGAGTGCGGCGATTTATACACAAACCACTGATGTCGAAGGTGAAGTAAATGGTCTGATTACTTATGACCG
GAAAGTTATTAAAATTCCGGAAGGCTTGTTGCATATCATGCACGATCGTTTATATCATGTCAATCCGGCGAAAGTCATTA
CTTTGGTGCCCGACGCTCAGAATGGGAGTGCGGTCAGCCGTGCAGTCAGCCTTAACGGGCAGGAGTCGAAAATGACCTCT
TTGCCTTTCGATTGCCCTCCCCGTTCGGAAATTGTGTCGGAAACTTCATTTGATGTCAATGATGAATTCAGCCATTTGTC
TTTGTGGGTGCGTGCTACCGGAGATGTGAAGGTATGGTTGAATGGAGTGGAGGTCTTTTCGCAGGAGGTGAAACAAACCA
GGCAATATAATCAGTACAATATCAGTAATTATTGCCGATATTTGCGAAAAGGAAAGAATGAGTTGAAAATCGAAGTACGG
GAAACTAAAAAGATGAGCTTTGATTTCGGTTTGAGGGCTTACTGA

Upstream 100 bases:

>100_bases
CCCGAAGGTTTGGAATACTTATAATAAAGGACGCCGGAAGGACTTTAAAGAATATAGAGATAAGTAGGAAAACACAGTAG
GAAAGAAAAGATAGGAACTT

Downstream 100 bases:

>100_bases
TCGGACACGTTTTTATTCCCAAGACTTGCAATAATTTCTCAAAGAAGGCTGTCGGTTTCTGAAAGACTTTTATCTTTGCA
GATATAATACATATGGCGCT

Product: beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 734; Mature: 734

Protein sequence:

>734_residues
MNKKLLIAVFVSAMAHTFACAASPKENRMKTVWADKVTSENVWQSYPRPQLQRSQWMNLNGLWQYAVTTQDTPKKEVKFE
GQILVPFAIESSLSGVQRSFLPTEKLWYQRNFTLDDAWKGKTVILHFGAVDYECQVWVNNKLAGIHKGGNNPFSFDVTKL
LRKGGSQLVEVAVIDPTDTESISRGKQQLEPKGIWYSPVSGIWQTVWLEAVNSTHILQVLPTADIHKKTVSLDISVAKAK
GGEKVKVELLDEGKVVGTTEQKLASKIEMEVPEAVLWSPSSPKLYHLNIELLSQGQVIDQVKSYFAMREVSIRKDECGYQ
RICLNGDPIFQYGTLDQGWWPDGLLTPPSEEAMLWDMVQLKNMGFNTIRKHIKVEPEQYYYYADSLGLMMWQDMVSGFST
ERKDEEHIKPNAQTDWNAPASHTNQWQQEMFEMIDRLRFYSCITTWVIFNEGWGQHNTVEIVEKVMEYDKSRIIDGVTGW
TDRGVGHMYDVHNYPSASMILPACNGNRISVLGEFGGYGWAIKEHLWNPGMRNWGYKNIDGAMALMDNYGRLVYDLETLI
AQGLSAAIYTQTTDVEGEVNGLITYDRKVIKIPEGLLHIMHDRLYHVNPAKVITLVPDAQNGSAVSRAVSLNGQESKMTS
LPFDCPPRSEIVSETSFDVNDEFSHLSLWVRATGDVKVWLNGVEVFSQEVKQTRQYNQYNISNYCRYLRKGKNELKIEVR
ETKKMSFDFGLRAY

Sequences:

>Translated_734_residues
MNKKLLIAVFVSAMAHTFACAASPKENRMKTVWADKVTSENVWQSYPRPQLQRSQWMNLNGLWQYAVTTQDTPKKEVKFE
GQILVPFAIESSLSGVQRSFLPTEKLWYQRNFTLDDAWKGKTVILHFGAVDYECQVWVNNKLAGIHKGGNNPFSFDVTKL
LRKGGSQLVEVAVIDPTDTESISRGKQQLEPKGIWYSPVSGIWQTVWLEAVNSTHILQVLPTADIHKKTVSLDISVAKAK
GGEKVKVELLDEGKVVGTTEQKLASKIEMEVPEAVLWSPSSPKLYHLNIELLSQGQVIDQVKSYFAMREVSIRKDECGYQ
RICLNGDPIFQYGTLDQGWWPDGLLTPPSEEAMLWDMVQLKNMGFNTIRKHIKVEPEQYYYYADSLGLMMWQDMVSGFST
ERKDEEHIKPNAQTDWNAPASHTNQWQQEMFEMIDRLRFYSCITTWVIFNEGWGQHNTVEIVEKVMEYDKSRIIDGVTGW
TDRGVGHMYDVHNYPSASMILPACNGNRISVLGEFGGYGWAIKEHLWNPGMRNWGYKNIDGAMALMDNYGRLVYDLETLI
AQGLSAAIYTQTTDVEGEVNGLITYDRKVIKIPEGLLHIMHDRLYHVNPAKVITLVPDAQNGSAVSRAVSLNGQESKMTS
LPFDCPPRSEIVSETSFDVNDEFSHLSLWVRATGDVKVWLNGVEVFSQEVKQTRQYNQYNISNYCRYLRKGKNELKIEVR
ETKKMSFDFGLRAY
>Mature_734_residues
MNKKLLIAVFVSAMAHTFACAASPKENRMKTVWADKVTSENVWQSYPRPQLQRSQWMNLNGLWQYAVTTQDTPKKEVKFE
GQILVPFAIESSLSGVQRSFLPTEKLWYQRNFTLDDAWKGKTVILHFGAVDYECQVWVNNKLAGIHKGGNNPFSFDVTKL
LRKGGSQLVEVAVIDPTDTESISRGKQQLEPKGIWYSPVSGIWQTVWLEAVNSTHILQVLPTADIHKKTVSLDISVAKAK
GGEKVKVELLDEGKVVGTTEQKLASKIEMEVPEAVLWSPSSPKLYHLNIELLSQGQVIDQVKSYFAMREVSIRKDECGYQ
RICLNGDPIFQYGTLDQGWWPDGLLTPPSEEAMLWDMVQLKNMGFNTIRKHIKVEPEQYYYYADSLGLMMWQDMVSGFST
ERKDEEHIKPNAQTDWNAPASHTNQWQQEMFEMIDRLRFYSCITTWVIFNEGWGQHNTVEIVEKVMEYDKSRIIDGVTGW
TDRGVGHMYDVHNYPSASMILPACNGNRISVLGEFGGYGWAIKEHLWNPGMRNWGYKNIDGAMALMDNYGRLVYDLETLI
AQGLSAAIYTQTTDVEGEVNGLITYDRKVIKIPEGLLHIMHDRLYHVNPAKVITLVPDAQNGSAVSRAVSLNGQESKMTS
LPFDCPPRSEIVSETSFDVNDEFSHLSLWVRATGDVKVWLNGVEVFSQEVKQTRQYNQYNISNYCRYLRKGKNELKIEVR
ETKKMSFDFGLRAY

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI48994920, Length=253, Percent_Identity=25.6916996047431, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787903, Length=413, Percent_Identity=23.728813559322, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI62471735, Length=447, Percent_Identity=24.8322147651007, Blast_Score=86, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922582, Length=447, Percent_Identity=24.8322147651007, Blast_Score=86, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24655438, Length=447, Percent_Identity=24.8322147651007, Blast_Score=86, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24651745, Length=448, Percent_Identity=22.9910714285714, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 83722; Mature: 83722

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKLLIAVFVSAMAHTFACAASPKENRMKTVWADKVTSENVWQSYPRPQLQRSQWMNLN
CCCCEEHHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCCCCHHHHHCCCCHHHHHHCCCCC
GLWQYAVTTQDTPKKEVKFEGQILVPFAIESSLSGVQRSFLPTEKLWYQRNFTLDDAWKG
CEEEEEEECCCCCCCCEEECCEEEEEEEHHHHHHHHHHHCCCHHHHHEECCCCCCCCCCC
KTVILHFGAVDYECQVWVNNKLAGIHKGGNNPFSFDVTKLLRKGGSQLVEVAVIDPTDTE
CEEEEEECCCCEEEEEEECCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCH
SISRGKQQLEPKGIWYSPVSGIWQTVWLEAVNSTHILQVLPTADIHKKTVSLDISVAKAK
HHHHHHHHCCCCCCEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEEEEECC
GGEKVKVELLDEGKVVGTTEQKLASKIEMEVPEAVLWSPSSPKLYHLNIELLSQGQVIDQ
CCCEEEEEEECCCCEECCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEECCCHHHHH
VKSYFAMREVSIRKDECGYQRICLNGDPIFQYGTLDQGWWPDGLLTPPSEEAMLWDMVQL
HHHHHHHHHHCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
KNMGFNTIRKHIKVEPEQYYYYADSLGLMMWQDMVSGFSTERKDEEHIKPNAQTDWNAPA
HHCCHHHHHHHEECCCHHEEEEECHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCC
SHTNQWQQEMFEMIDRLRFYSCITTWVIFNEGWGQHNTVEIVEKVMEYDKSRIIDGVTGW
CHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
TDRGVGHMYDVHNYPSASMILPACNGNRISVLGEFGGYGWAIKEHLWNPGMRNWGYKNID
CCCCCCCEEECCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCC
GAMALMDNYGRLVYDLETLIAQGLSAAIYTQTTDVEGEVNGLITYDRKVIKIPEGLLHIM
HHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCEEEECCHHHHHHH
HDRLYHVNPAKVITLVPDAQNGSAVSRAVSLNGQESKMTSLPFDCPPRSEIVSETSFDVN
HHHHEEECCCEEEEEECCCCCCCHHHHEEECCCCCCHHCCCCCCCCCHHHHHCCCCCCCC
DEFSHLSLWVRATGDVKVWLNGVEVFSQEVKQTRQYNQYNISNYCRYLRKGKNELKIEVR
CCCCEEEEEEEECCCEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEE
ETKKMSFDFGLRAY
ECCCEEECCCCCCC
>Mature Secondary Structure
MNKKLLIAVFVSAMAHTFACAASPKENRMKTVWADKVTSENVWQSYPRPQLQRSQWMNLN
CCCCEEHHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCCCCHHHHHCCCCHHHHHHCCCCC
GLWQYAVTTQDTPKKEVKFEGQILVPFAIESSLSGVQRSFLPTEKLWYQRNFTLDDAWKG
CEEEEEEECCCCCCCCEEECCEEEEEEEHHHHHHHHHHHCCCHHHHHEECCCCCCCCCCC
KTVILHFGAVDYECQVWVNNKLAGIHKGGNNPFSFDVTKLLRKGGSQLVEVAVIDPTDTE
CEEEEEECCCCEEEEEEECCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCH
SISRGKQQLEPKGIWYSPVSGIWQTVWLEAVNSTHILQVLPTADIHKKTVSLDISVAKAK
HHHHHHHHCCCCCCEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEEEEECC
GGEKVKVELLDEGKVVGTTEQKLASKIEMEVPEAVLWSPSSPKLYHLNIELLSQGQVIDQ
CCCEEEEEEECCCCEECCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEECCCHHHHH
VKSYFAMREVSIRKDECGYQRICLNGDPIFQYGTLDQGWWPDGLLTPPSEEAMLWDMVQL
HHHHHHHHHHCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
KNMGFNTIRKHIKVEPEQYYYYADSLGLMMWQDMVSGFSTERKDEEHIKPNAQTDWNAPA
HHCCHHHHHHHEECCCHHEEEEECHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCC
SHTNQWQQEMFEMIDRLRFYSCITTWVIFNEGWGQHNTVEIVEKVMEYDKSRIIDGVTGW
CHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
TDRGVGHMYDVHNYPSASMILPACNGNRISVLGEFGGYGWAIKEHLWNPGMRNWGYKNID
CCCCCCCEEECCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCC
GAMALMDNYGRLVYDLETLIAQGLSAAIYTQTTDVEGEVNGLITYDRKVIKIPEGLLHIM
HHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCEEEECCHHHHHHH
HDRLYHVNPAKVITLVPDAQNGSAVSRAVSLNGQESKMTSLPFDCPPRSEIVSETSFDVN
HHHHEEECCCEEEEEECCCCCCCHHHHEEECCCCCCHHCCCCCCCCCHHHHHCCCCCCCC
DEFSHLSLWVRATGDVKVWLNGVEVFSQEVKQTRQYNQYNISNYCRYLRKGKNELKIEVR
CCCCEEEEEEEECCCEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEE
ETKKMSFDFGLRAY
ECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA