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The map label for this gene is rfbB [C]

Identifier: 29348289

GI number: 29348289

Start: 3600203

End: 3601237

Strand: Reverse

Name: rfbB [C]

Synonym: BT_2880

Alternate gene names: 29348289

Gene position: 3601237-3600203 (Counterclockwise)

Preceding gene: 29348290

Following gene: 29348288

Centisome position: 57.52

GC content: 40.0

Gene sequence:

>1035_bases
ATGATGACTTATTATGACGATATCAGAAAAGTGCAGGATTTGCATCTGCCGTGGGAAATTCTGACTGATATAAATATTCT
GATTGTGGGGGCCTCAGGTCTGATAGGAAGGGCGTTGGTGGATACTCTGATGCAGTTACCCGATAAGACGTTTCATCTAT
ATGCAGGTGTACGGGATTTGGTTTATGCGCAAAGTTGTTTCATGCGATACAAGGATGATGAATCTTTCACCTTGATACAA
TGTGATGTGACAGCATTGATTCCATTTGATATTGATTTTCACTATATTATTCATGCAGCGAGCTATGCGGGGCCGGCCGC
CTTTCATAATGATCCGGTCGGGGTTATGAAAGCGAATATATTGGGAGTTGACAATCTGTTTTCATACGGAGAGCAACACA
ATCTAAGAAGGTTGTTATATGTTTCTTCCGGTGAAGTGTATGGAGAGGGGAATGGAATTCCTTTTCGCGAAGAAGACAGT
GGTTCTCTCGATTGGGCTTCCTTACGCGCATGTTACCCTGCGGCAAAACGGACTGCTGAGACTTTGTGTATTGCTTATGC
AGCTCAATTTCAGATAGAAACAGTTATAGCACGTCCTTGCCATATTTATGGACCTTTTTACACTTCAAAAGATGATCGTG
CCTATGCGCAGTTTATACGAAATGTATTGGCAGGTGAAAATATAATATTGAGAAGTTCTGGTTTGCAACAGCGTTCCTGG
TGTTATGTGGTTGATTGTGTGTTTGCACTTCTTTACGTTTTGCTAAAAGGAAAAACAAAGAATGCTTATAATATAGCTGA
CGTCCGGTCGAATGTCAGTATCCGCGAATTTGCGGAAATGATAGCACTGAAAAGTGAACGAGAAGTAATTTTCGATTTAC
CGGATAATACCGGGAAGAATAAACCTATTATATCACAGGCGATATTTAATACTGAGAAAATAAATAAAATAGGCTGGTTC
CCCCAATGGAAGCTTGAAGAAGGCGTTTCACATACACTTAATACTTTAATATCTGTTGATGGAACATATATATAA

Upstream 100 bases:

>100_bases
ATATAGTTCCGGGAGAGGAGCGAAATATAAAGATTACGACTACGGAAGACCTGGAGATTTTCAAGGTTTTGAGGCAAACG
TCAAAAGAAGACTGGCTGAA

Downstream 100 bases:

>100_bases
GTTGCTGCGCTCTTTTAAATGGGACTGTGCTAAATATCTCTACAAGAATACTCTGAACTTTAAAGTTAAGAGATTAAGAC
GAAAGAAAAATATAAGAGTT

Product: putative nucleotide-sugar dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MMTYYDDIRKVQDLHLPWEILTDINILIVGASGLIGRALVDTLMQLPDKTFHLYAGVRDLVYAQSCFMRYKDDESFTLIQ
CDVTALIPFDIDFHYIIHAASYAGPAAFHNDPVGVMKANILGVDNLFSYGEQHNLRRLLYVSSGEVYGEGNGIPFREEDS
GSLDWASLRACYPAAKRTAETLCIAYAAQFQIETVIARPCHIYGPFYTSKDDRAYAQFIRNVLAGENIILRSSGLQQRSW
CYVVDCVFALLYVLLKGKTKNAYNIADVRSNVSIREFAEMIALKSEREVIFDLPDNTGKNKPIISQAIFNTEKINKIGWF
PQWKLEEGVSHTLNTLISVDGTYI

Sequences:

>Translated_344_residues
MMTYYDDIRKVQDLHLPWEILTDINILIVGASGLIGRALVDTLMQLPDKTFHLYAGVRDLVYAQSCFMRYKDDESFTLIQ
CDVTALIPFDIDFHYIIHAASYAGPAAFHNDPVGVMKANILGVDNLFSYGEQHNLRRLLYVSSGEVYGEGNGIPFREEDS
GSLDWASLRACYPAAKRTAETLCIAYAAQFQIETVIARPCHIYGPFYTSKDDRAYAQFIRNVLAGENIILRSSGLQQRSW
CYVVDCVFALLYVLLKGKTKNAYNIADVRSNVSIREFAEMIALKSEREVIFDLPDNTGKNKPIISQAIFNTEKINKIGWF
PQWKLEEGVSHTLNTLISVDGTYI
>Mature_344_residues
MMTYYDDIRKVQDLHLPWEILTDINILIVGASGLIGRALVDTLMQLPDKTFHLYAGVRDLVYAQSCFMRYKDDESFTLIQ
CDVTALIPFDIDFHYIIHAASYAGPAAFHNDPVGVMKANILGVDNLFSYGEQHNLRRLLYVSSGEVYGEGNGIPFREEDS
GSLDWASLRACYPAAKRTAETLCIAYAAQFQIETVIARPCHIYGPFYTSKDDRAYAQFIRNVLAGENIILRSSGLQQRSW
CYVVDCVFALLYVLLKGKTKNAYNIADVRSNVSIREFAEMIALKSEREVIFDLPDNTGKNKPIISQAIFNTEKINKIGWF
PQWKLEEGVSHTLNTLISVDGTYI

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=314, Percent_Identity=31.2101910828025, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI7657641, Length=321, Percent_Identity=24.6105919003115, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=24.5070422535211, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48994969, Length=339, Percent_Identity=24.4837758112094, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17539532, Length=317, Percent_Identity=29.3375394321767, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI115532424, Length=338, Percent_Identity=24.8520710059172, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=259, Percent_Identity=24.7104247104247, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI21356223, Length=320, Percent_Identity=30, Blast_Score=118, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38888; Mature: 38888

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTYYDDIRKVQDLHLPWEILTDINILIVGASGLIGRALVDTLMQLPDKTFHLYAGVRDL
CCCHHHHHHHHHHCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHH
VYAQSCFMRYKDDESFTLIQCDVTALIPFDIDFHYIIHAASYAGPAAFHNDPVGVMKANI
HHHHHHHHEECCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEEEE
LGVDNLFSYGEQHNLRRLLYVSSGEVYGEGNGIPFREEDSGSLDWASLRACYPAAKRTAE
ECHHHHHHCCCCCCCEEEEEEECCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TLCIAYAAQFQIETVIARPCHIYGPFYTSKDDRAYAQFIRNVLAGENIILRSSGLQQRSW
HHHHHHHHHHEEEEHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCC
CYVVDCVFALLYVLLKGKTKNAYNIADVRSNVSIREFAEMIALKSEREVIFDLPDNTGKN
HHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCCCC
KPIISQAIFNTEKINKIGWFPQWKLEEGVSHTLNTLISVDGTYI
CCHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMTYYDDIRKVQDLHLPWEILTDINILIVGASGLIGRALVDTLMQLPDKTFHLYAGVRDL
CCCHHHHHHHHHHCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHH
VYAQSCFMRYKDDESFTLIQCDVTALIPFDIDFHYIIHAASYAGPAAFHNDPVGVMKANI
HHHHHHHHEECCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEEEE
LGVDNLFSYGEQHNLRRLLYVSSGEVYGEGNGIPFREEDSGSLDWASLRACYPAAKRTAE
ECHHHHHHCCCCCCCEEEEEEECCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TLCIAYAAQFQIETVIARPCHIYGPFYTSKDDRAYAQFIRNVLAGENIILRSSGLQQRSW
HHHHHHHHHHEEEEHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCC
CYVVDCVFALLYVLLKGKTKNAYNIADVRSNVSIREFAEMIALKSEREVIFDLPDNTGKN
HHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCCCC
KPIISQAIFNTEKINKIGWFPQWKLEEGVSHTLNTLISVDGTYI
CCHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]