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The map label for this gene is rpoN [H]

Identifier: 29347931

GI number: 29347931

Start: 3144919

End: 3146403

Strand: Reverse

Name: rpoN [H]

Synonym: BT_2521

Alternate gene names: 29347931

Gene position: 3146403-3144919 (Counterclockwise)

Preceding gene: 29347936

Following gene: 29347930

Centisome position: 50.26

GC content: 48.89

Gene sequence:

>1485_bases
ATGGCACAAGGTTCCCGTCAAATACAATCGCAGGCGCAACAGCAGATACAAACGCTATCGCCACAGCAGATTCTGGTCGT
GAAGTTGTTGGAGCTTCCCGCAGTTGAGCTGGAAGACCGTATCCATGCTGAACTATTGGAAAATCCGGCGCTTGAAGAAG
GCAAAGAAGACGCTGCTACAGATGAATATGCCGGTGGCGTCAGTCCGGAAGAAGGCATGGATAACGATGCCAATGATTAT
GATTCTTTAAGTGATTATCTCACCGAAGACGATATCCCCGACTATAAATTGCAGGAGAACAACCGCTCGCGGGACGAGCA
GGCGGAAGATATTCCTTTCTCCGACGCCACCTCTTTTTATGAAATCCTGAAAGAACAACTCCGTGAACGTAACCTGACCG
AACACCAATGCGAACTGGTGGAATATCTGATCGGTTCGCTGGACGATGACGGACTGCTCCGTAAATCGCTGGAAAGTATC
TGTGACGAACTGGCTATCTACGCCGGTATCGAATCTACGGAAGAAGAACTGGAAGAGGCTCTGTGCATCCTGCAGGACTT
TGACCCGGCAGGTATCGGAGCACGCAACCTTCAGGAATGTCTGATGATACAGATTCTCCGCAAGAAGAGCGAAGAGAAGA
AGCCGTCGCCGATATTGAACCTGGAAGAGCGCATCATCAGTGACTGTTATGAAGAATTTACCCGGAAGCACTGGGAGAAA
ATCATTAAGAAACTGGATGTCAGAGAAGAGACTTTCAATGAGGCGATTGCCGAAATAACCAAGTTGAATCCCCGTCCGGG
CGCTTCGCTCGGAGAGACCATCGGAAGAAACCTACAGCAGATTGTTCCCGATTTTCTGGTGGATACCTACGATGACGGAA
CGATTAACGTAAGCCTGAATAACCGCAATGTTCCCGAACTCCGGATGAGCCGCGACTTTACCGAGATGGTGGAGGAGCAT
ACCAAAAATAAGGCGAACCAGTCCAAAGAGTCGAAAGAAGCCATGATGTTCCTGAAACAGAAGATGGATGCGGCACAAGG
ATTTATCGATGCGGTGAAGCAGCGGCAGAATACATTGATGACTACCATGCAGGCGATTATCGACTTGCAACGTCCTTTCT
TCCTCGAAGGCGACGAGTCATTGCTGAAACCGATGATTCTCAAAGATGTGGCAGAACGTACCGGACTGGATATCTCTACG
ATTTCCCGTGTCAGCAACAGCAAATACGTACAGACCAACTTCGGCATCTATCCGCTTAAATTCTTCTTCAGCGATGGCTA
TACGACGGAAGACGGAGAGGAAATGTCCGTACGCGAGATACGTAAGATTCTGAAAGAATGCATTGACGGAGAAGACAAAA
AGAAACCGCTGACAGATGACGAACTGGCGGATATCCTGAAGGAAAAAGGCTATCCTATCGCCCGCCGTACGGTGGCGAAA
TACCGTCAGCAGTTGAATATACCGGTGGCAAGACTGAGAAAGTGA

Upstream 100 bases:

>100_bases
AATTTTGTGTTATAGGATTAAAAATAATCCCACTAATCTGTGTAATCTCTCGTTAAAAAGTTACTTTTGTAGCGAGAAAC
AGTAGAAAGCAAAAACGAAT

Downstream 100 bases:

>100_bases
GTTTAAAGTATTAACTATAAAGTATTAAGTATTAGGTGCTGATGGATAAAGAAAAAGAGCATATCATAGCGGATAAAACA
ATGTTGCGGGTGGCACGGAT

Product: RNA polymerase sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MAQGSRQIQSQAQQQIQTLSPQQILVVKLLELPAVELEDRIHAELLENPALEEGKEDAATDEYAGGVSPEEGMDNDANDY
DSLSDYLTEDDIPDYKLQENNRSRDEQAEDIPFSDATSFYEILKEQLRERNLTEHQCELVEYLIGSLDDDGLLRKSLESI
CDELAIYAGIESTEEELEEALCILQDFDPAGIGARNLQECLMIQILRKKSEEKKPSPILNLEERIISDCYEEFTRKHWEK
IIKKLDVREETFNEAIAEITKLNPRPGASLGETIGRNLQQIVPDFLVDTYDDGTINVSLNNRNVPELRMSRDFTEMVEEH
TKNKANQSKESKEAMMFLKQKMDAAQGFIDAVKQRQNTLMTTMQAIIDLQRPFFLEGDESLLKPMILKDVAERTGLDIST
ISRVSNSKYVQTNFGIYPLKFFFSDGYTTEDGEEMSVREIRKILKECIDGEDKKKPLTDDELADILKEKGYPIARRTVAK
YRQQLNIPVARLRK

Sequences:

>Translated_494_residues
MAQGSRQIQSQAQQQIQTLSPQQILVVKLLELPAVELEDRIHAELLENPALEEGKEDAATDEYAGGVSPEEGMDNDANDY
DSLSDYLTEDDIPDYKLQENNRSRDEQAEDIPFSDATSFYEILKEQLRERNLTEHQCELVEYLIGSLDDDGLLRKSLESI
CDELAIYAGIESTEEELEEALCILQDFDPAGIGARNLQECLMIQILRKKSEEKKPSPILNLEERIISDCYEEFTRKHWEK
IIKKLDVREETFNEAIAEITKLNPRPGASLGETIGRNLQQIVPDFLVDTYDDGTINVSLNNRNVPELRMSRDFTEMVEEH
TKNKANQSKESKEAMMFLKQKMDAAQGFIDAVKQRQNTLMTTMQAIIDLQRPFFLEGDESLLKPMILKDVAERTGLDIST
ISRVSNSKYVQTNFGIYPLKFFFSDGYTTEDGEEMSVREIRKILKECIDGEDKKKPLTDDELADILKEKGYPIARRTVAK
YRQQLNIPVARLRK
>Mature_493_residues
AQGSRQIQSQAQQQIQTLSPQQILVVKLLELPAVELEDRIHAELLENPALEEGKEDAATDEYAGGVSPEEGMDNDANDYD
SLSDYLTEDDIPDYKLQENNRSRDEQAEDIPFSDATSFYEILKEQLRERNLTEHQCELVEYLIGSLDDDGLLRKSLESIC
DELAIYAGIESTEEELEEALCILQDFDPAGIGARNLQECLMIQILRKKSEEKKPSPILNLEERIISDCYEEFTRKHWEKI
IKKLDVREETFNEAIAEITKLNPRPGASLGETIGRNLQQIVPDFLVDTYDDGTINVSLNNRNVPELRMSRDFTEMVEEHT
KNKANQSKESKEAMMFLKQKMDAAQGFIDAVKQRQNTLMTTMQAIIDLQRPFFLEGDESLLKPMILKDVAERTGLDISTI
SRVSNSKYVQTNFGIYPLKFFFSDGYTTEDGEEMSVREIRKILKECIDGEDKKKPLTDDELADILKEKGYPIARRTVAKY
RQQLNIPVARLRK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=528, Percent_Identity=31.6287878787879, Blast_Score=211, Evalue=1e-55,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 56569; Mature: 56438

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQGSRQIQSQAQQQIQTLSPQQILVVKLLELPAVELEDRIHAELLENPALEEGKEDAAT
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHCCCCCCCC
DEYAGGVSPEEGMDNDANDYDSLSDYLTEDDIPDYKLQENNRSRDEQAEDIPFSDATSFY
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHH
EILKEQLRERNLTEHQCELVEYLIGSLDDDGLLRKSLESICDELAIYAGIESTEEELEEA
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LCILQDFDPAGIGARNLQECLMIQILRKKSEEKKPSPILNLEERIISDCYEEFTRKHWEK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IIKKLDVREETFNEAIAEITKLNPRPGASLGETIGRNLQQIVPDFLVDTYDDGTINVSLN
HHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
NRNVPELRMSRDFTEMVEEHTKNKANQSKESKEAMMFLKQKMDAAQGFIDAVKQRQNTLM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTMQAIIDLQRPFFLEGDESLLKPMILKDVAERTGLDISTISRVSNSKYVQTNFGIYPLK
HHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEECCCCEEHH
FFFSDGYTTEDGEEMSVREIRKILKECIDGEDKKKPLTDDELADILKEKGYPIARRTVAK
HEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
YRQQLNIPVARLRK
HHHHCCCCHHHHCC
>Mature Secondary Structure 
AQGSRQIQSQAQQQIQTLSPQQILVVKLLELPAVELEDRIHAELLENPALEEGKEDAAT
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHCCCCCCCC
DEYAGGVSPEEGMDNDANDYDSLSDYLTEDDIPDYKLQENNRSRDEQAEDIPFSDATSFY
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHH
EILKEQLRERNLTEHQCELVEYLIGSLDDDGLLRKSLESICDELAIYAGIESTEEELEEA
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LCILQDFDPAGIGARNLQECLMIQILRKKSEEKKPSPILNLEERIISDCYEEFTRKHWEK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IIKKLDVREETFNEAIAEITKLNPRPGASLGETIGRNLQQIVPDFLVDTYDDGTINVSLN
HHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
NRNVPELRMSRDFTEMVEEHTKNKANQSKESKEAMMFLKQKMDAAQGFIDAVKQRQNTLM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTMQAIIDLQRPFFLEGDESLLKPMILKDVAERTGLDISTISRVSNSKYVQTNFGIYPLK
HHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEECCCCEEHH
FFFSDGYTTEDGEEMSVREIRKILKECIDGEDKKKPLTDDELADILKEKGYPIARRTVAK
HEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
YRQQLNIPVARLRK
HHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2198257 [H]