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The map label for this gene is galE [C]

Identifier: 29346752

GI number: 29346752

Start: 1667563

End: 1668624

Strand: Reverse

Name: galE [C]

Synonym: BT_1342

Alternate gene names: 29346752

Gene position: 1668624-1667563 (Counterclockwise)

Preceding gene: 29346753

Following gene: 29346751

Centisome position: 26.65

GC content: 35.22

Gene sequence:

>1062_bases
ATGAAAGTATTAGTTACAGGGGCTGCTGGATTTATTGGTAGTTTTACTTCAAAAAAGCTGTTAGAGCAAGGACATGAAGT
TGTGGGTTTGGATAATCTGAATGATTATTATGATACTAGTTTGAAATATGGACGTTTGGCGAATTTAGGGATTAATAAAG
ATGAAATAGGTTGGTATAAATATACTCGAAGTTCTTATTATACAGCCTTCAACTTTATTCGTATGAATCTTGAAGATAGG
CAAGCAGTACAAATGTTGTTTGCAAATGGCGATTTTGATTGTGTATGTAATTTGGCTGCTCAAGCAGGGGTGCGCTATTC
TATTGAGAATCCTTATGCATATATTGAAAGTAACATAGATGGCTTTTTGAATATATTGGAAGGGTGCCGAAATTCAAAGG
TTAGCCATCTTATTTATGCCAGCTCAAGTAGTGTTTATGGTTTAAATGGTAATGTACCTTTTTCAGAGAAAGATAGTATT
GCTCATCCTGTTAGTTTGTACGCTGCTTCAAAGAAAGCTGATGAATTAATGGCTCATGCATATAGCCATTTATATAATAT
TCCGACTACTGGATTGCGCTTCTTTACCGTTTATGGTCCATGGGGACGGCCAGATATGTCTCCATTCTTATTTGCTGATG
CAATTCTTCATCATCGGGCTATTAAAGTTTTTAATAACGGAAATATGTTTCGTGACTTTACGTATATTGATGATATAATC
GAAGGAATAATCAGGGTTATGCAACATGTTCCAATAGGTAATAAGAATTGGAAGCCTGAAAACCCTTCCCCTGATTCTTC
TTCAGCACCTTATAAAATATATAATATTGGAAACTCTCGTCCTGTGAAACTAATGGATTTTATCCAAGCTATTGAAAATG
CGATAGGTAGAGAGGCGGAAAAAATATATCTTCCGATGCAGCCTGGAGATGTATATCAAACGTATGCCGATATAACTAGT
CTTGAGCATGATACAGGATTTAAGCCAGATACATCTTTGCTTGAGGGAGTGAGGAAAACAATAGATTGGTATTGTGATTT
TTATAAATTAGATAGTAAATAG

Upstream 100 bases:

>100_bases
ATTATGGATGTAAATCTTCATACAAAGAATCTTGGTGAGTTCAAATTATATCCATTAACTTCTTTGTTTTGAGATAGAGT
TTTTATAGATAAATTTTATA

Downstream 100 bases:

>100_bases
TTGTATTATGAATATTGCAGTAGTAGGAACCGGATATGTTGGTTTAGTCTCAGGAGCATGTTTCTCGGAAATAGGAGTAA
ATGTTACATGCATAGATGTT

Product: putative UDP-glucuronic acid epimerase

Products: UDPglucoseal [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKVLVTGAAGFIGSFTSKKLLEQGHEVVGLDNLNDYYDTSLKYGRLANLGINKDEIGWYKYTRSSYYTAFNFIRMNLEDR
QAVQMLFANGDFDCVCNLAAQAGVRYSIENPYAYIESNIDGFLNILEGCRNSKVSHLIYASSSSVYGLNGNVPFSEKDSI
AHPVSLYAASKKADELMAHAYSHLYNIPTTGLRFFTVYGPWGRPDMSPFLFADAILHHRAIKVFNNGNMFRDFTYIDDII
EGIIRVMQHVPIGNKNWKPENPSPDSSSAPYKIYNIGNSRPVKLMDFIQAIENAIGREAEKIYLPMQPGDVYQTYADITS
LEHDTGFKPDTSLLEGVRKTIDWYCDFYKLDSK

Sequences:

>Translated_353_residues
MKVLVTGAAGFIGSFTSKKLLEQGHEVVGLDNLNDYYDTSLKYGRLANLGINKDEIGWYKYTRSSYYTAFNFIRMNLEDR
QAVQMLFANGDFDCVCNLAAQAGVRYSIENPYAYIESNIDGFLNILEGCRNSKVSHLIYASSSSVYGLNGNVPFSEKDSI
AHPVSLYAASKKADELMAHAYSHLYNIPTTGLRFFTVYGPWGRPDMSPFLFADAILHHRAIKVFNNGNMFRDFTYIDDII
EGIIRVMQHVPIGNKNWKPENPSPDSSSAPYKIYNIGNSRPVKLMDFIQAIENAIGREAEKIYLPMQPGDVYQTYADITS
LEHDTGFKPDTSLLEGVRKTIDWYCDFYKLDSK
>Mature_353_residues
MKVLVTGAAGFIGSFTSKKLLEQGHEVVGLDNLNDYYDTSLKYGRLANLGINKDEIGWYKYTRSSYYTAFNFIRMNLEDR
QAVQMLFANGDFDCVCNLAAQAGVRYSIENPYAYIESNIDGFLNILEGCRNSKVSHLIYASSSSVYGLNGNVPFSEKDSI
AHPVSLYAASKKADELMAHAYSHLYNIPTTGLRFFTVYGPWGRPDMSPFLFADAILHHRAIKVFNNGNMFRDFTYIDDII
EGIIRVMQHVPIGNKNWKPENPSPDSSSAPYKIYNIGNSRPVKLMDFIQAIENAIGREAEKIYLPMQPGDVYQTYADITS
LEHDTGFKPDTSLLEGVRKTIDWYCDFYKLDSK

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=352, Percent_Identity=27.8409090909091, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI56237023, Length=350, Percent_Identity=25.1428571428571, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI56118217, Length=350, Percent_Identity=25.1428571428571, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI189083684, Length=350, Percent_Identity=25.1428571428571, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI42516563, Length=353, Percent_Identity=26.9121813031161, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1786974, Length=361, Percent_Identity=22.9916897506925, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI48994969, Length=368, Percent_Identity=25.5434782608696, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788353, Length=371, Percent_Identity=26.1455525606469, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788365, Length=286, Percent_Identity=23.7762237762238, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71982035, Length=368, Percent_Identity=24.7282608695652, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71982038, Length=366, Percent_Identity=24.3169398907104, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17539532, Length=354, Percent_Identity=27.4011299435028, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17568069, Length=364, Percent_Identity=26.3736263736264, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI115532424, Length=356, Percent_Identity=21.6292134831461, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=281, Percent_Identity=28.1138790035587, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19923002, Length=359, Percent_Identity=25.3481894150418, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356223, Length=353, Percent_Identity=25.2124645892351, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 39917; Mature: 39917

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVTGAAGFIGSFTSKKLLEQGHEVVGLDNLNDYYDTSLKYGRLANLGINKDEIGWYK
CEEEEEECCHHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCCCCCEECCCCCCCCCCCEE
YTRSSYYTAFNFIRMNLEDRQAVQMLFANGDFDCVCNLAAQAGVRYSIENPYAYIESNID
EECCCHHEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCEEEECCCHH
GFLNILEGCRNSKVSHLIYASSSSVYGLNGNVPFSEKDSIAHPVSLYAASKKADELMAHA
HHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
YSHLYNIPTTGLRFFTVYGPWGRPDMSPFLFADAILHHRAIKVFNNGNMFRDFTYIDDII
HHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHHH
EGIIRVMQHVPIGNKNWKPENPSPDSSSAPYKIYNIGNSRPVKLMDFIQAIENAIGREAE
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCC
KIYLPMQPGDVYQTYADITSLEHDTGFKPDTSLLEGVRKTIDWYCDFYKLDSK
EEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCC
>Mature Secondary Structure
MKVLVTGAAGFIGSFTSKKLLEQGHEVVGLDNLNDYYDTSLKYGRLANLGINKDEIGWYK
CEEEEEECCHHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCCCCCEECCCCCCCCCCCEE
YTRSSYYTAFNFIRMNLEDRQAVQMLFANGDFDCVCNLAAQAGVRYSIENPYAYIESNID
EECCCHHEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCEEEECCCHH
GFLNILEGCRNSKVSHLIYASSSSVYGLNGNVPFSEKDSIAHPVSLYAASKKADELMAHA
HHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
YSHLYNIPTTGLRFFTVYGPWGRPDMSPFLFADAILHHRAIKVFNNGNMFRDFTYIDDII
HHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHHH
EGIIRVMQHVPIGNKNWKPENPSPDSSSAPYKIYNIGNSRPVKLMDFIQAIENAIGREAE
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCC
KIYLPMQPGDVYQTYADITSLEHDTGFKPDTSLLEGVRKTIDWYCDFYKLDSK
EEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]