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The map label for this gene is paaK [H]

Identifier: 29345838

GI number: 29345838

Start: 514550

End: 515857

Strand: Reverse

Name: paaK [H]

Synonym: BT_0428

Alternate gene names: 29345838

Gene position: 515857-514550 (Counterclockwise)

Preceding gene: 29345839

Following gene: 29345837

Centisome position: 8.24

GC content: 47.4

Gene sequence:

>1308_bases
ATGAGTACACAATACTGGGAAGAAGAAATTGAAATCATGAGTCGCGAGAAGTTGCAGGAACTGCAACTTCAGCGACTCAA
AAAAACGATTAACATAGCCGCAAACTCACCTTATTACAAAGAGGTTTTCAGCAAAAACGGCATCACCGGAGATAGTATCC
AGTCACTGGACGACATCCGCAAAATTCCTTTCACCACTAAATCGGACATGCGTGCCAACTATCCCTTCGGGCTAGTGGCC
GGCGACATGAAAAGAGACGGTGTACGTATCCACTCTTCCAGTGGAACTACAGGAAACCCTACAGTCATCGTACATTCACA
ACATGACCTCGACTCATGGGCAAATCTGGTAGCCCGTTGCCTTTACATGGTGGGGATCCGCAAGACAGATGTCTTCCAGA
ACAGCTCCGGATATGGAATGTTTACAGGTGGATTAGGTTTTCAATATGGTGCAGAACGCCTTGGCTGCCTTACCGTACCT
GCCGCAGCCGGAAACAGTAAACGGCAAATTAAATTCATCAGTGACTTTAAGACTACCGCCTTACACGCCATCCCAAGCTA
CGCTATCCGTCTTGCCGAAGTATTTCAGGAAGAAGGTATCGACCCGAGAGAAACAACCCTGAAAACCTTGGTGATCGGCG
CCGAACCGCATACTGACGAACAGCGCCGTAAAATAGAACGGATGCTCAATGTGAAAGCATATAATAGTTTCGGTATGACG
GAAATGAACGGCCCCGGTGTTGCCTTCGAATGCCAGGAGCAGAACGGAATGCACTTCTGGGAAGATTGCTATCTGGTAGA
AATCATCGATCCTGAAACAGGAGAACCCGTTCCCGAAGGAGAAATCGGTGAATTGGTACTCACTACACTTGATCGTGAAA
TGATGCCGCTTATCCGCTACCGCACCCGCGACCTTACCCGTATACTACCCGGAAAATGTCCGTGCGGCCGCACCCATCTC
CGCATCGACCGGATCAAGGGACGCAGCGACGATATGTTTATCATCAAGGGAGTCAACATCTTCCCCATGCAAGTCGAAAA
GATTCTGGTTCAGTTCCCCGAATTAGGCAGCAATTATCTGATTACGCTGGAGACAGTCAACAATCAGGATGAGATGATCG
TCGAAGTGGAATTAAGCGATCTTTCTACAGACAACTATATCGAACTGGAAAAGATCCGCAGAGATATTATCCGTCAATTA
AAAGATGAAATACTGGTTACCCCGAAAGTAAAACTAGTAAAGAAAGGGTCTCTCCCGCAGAGCGAGGGAAAAGCCGTCAG
AGTGAAGGACTTAAGAGACAACAAATAA

Upstream 100 bases:

>100_bases
TCTTCCTGCGCAAAGGAGAAGCTATTGTCGAAATGAATCTTAAAGCACTGGCTGCCGGCAAGGAAATTGCGGAAAAGCTA
ATGCAATAACTATCACAATC

Downstream 100 bases:

>100_bases
ATATTCAAATGCCCGATGTGCTAATATGCCAACCCAATACTGTATGATATCAGTAACAAATGGCATATTAGCACATTATT
TAATTATCAGATTATAATGC

Product: phenylacetate-coenzyme A ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSTQYWEEEIEIMSREKLQELQLQRLKKTINIAANSPYYKEVFSKNGITGDSIQSLDDIRKIPFTTKSDMRANYPFGLVA
GDMKRDGVRIHSSSGTTGNPTVIVHSQHDLDSWANLVARCLYMVGIRKTDVFQNSSGYGMFTGGLGFQYGAERLGCLTVP
AAAGNSKRQIKFISDFKTTALHAIPSYAIRLAEVFQEEGIDPRETTLKTLVIGAEPHTDEQRRKIERMLNVKAYNSFGMT
EMNGPGVAFECQEQNGMHFWEDCYLVEIIDPETGEPVPEGEIGELVLTTLDREMMPLIRYRTRDLTRILPGKCPCGRTHL
RIDRIKGRSDDMFIIKGVNIFPMQVEKILVQFPELGSNYLITLETVNNQDEMIVEVELSDLSTDNYIELEKIRRDIIRQL
KDEILVTPKVKLVKKGSLPQSEGKAVRVKDLRDNK

Sequences:

>Translated_435_residues
MSTQYWEEEIEIMSREKLQELQLQRLKKTINIAANSPYYKEVFSKNGITGDSIQSLDDIRKIPFTTKSDMRANYPFGLVA
GDMKRDGVRIHSSSGTTGNPTVIVHSQHDLDSWANLVARCLYMVGIRKTDVFQNSSGYGMFTGGLGFQYGAERLGCLTVP
AAAGNSKRQIKFISDFKTTALHAIPSYAIRLAEVFQEEGIDPRETTLKTLVIGAEPHTDEQRRKIERMLNVKAYNSFGMT
EMNGPGVAFECQEQNGMHFWEDCYLVEIIDPETGEPVPEGEIGELVLTTLDREMMPLIRYRTRDLTRILPGKCPCGRTHL
RIDRIKGRSDDMFIIKGVNIFPMQVEKILVQFPELGSNYLITLETVNNQDEMIVEVELSDLSTDNYIELEKIRRDIIRQL
KDEILVTPKVKLVKKGSLPQSEGKAVRVKDLRDNK
>Mature_434_residues
STQYWEEEIEIMSREKLQELQLQRLKKTINIAANSPYYKEVFSKNGITGDSIQSLDDIRKIPFTTKSDMRANYPFGLVAG
DMKRDGVRIHSSSGTTGNPTVIVHSQHDLDSWANLVARCLYMVGIRKTDVFQNSSGYGMFTGGLGFQYGAERLGCLTVPA
AAGNSKRQIKFISDFKTTALHAIPSYAIRLAEVFQEEGIDPRETTLKTLVIGAEPHTDEQRRKIERMLNVKAYNSFGMTE
MNGPGVAFECQEQNGMHFWEDCYLVEIIDPETGEPVPEGEIGELVLTTLDREMMPLIRYRTRDLTRILPGKCPCGRTHLR
IDRIKGRSDDMFIIKGVNIFPMQVEKILVQFPELGSNYLITLETVNNQDEMIVEVELSDLSTDNYIELEKIRRDIIRQLK
DEILVTPKVKLVKKGSLPQSEGKAVRVKDLRDNK

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=430, Percent_Identity=44.6511627906977, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 49380; Mature: 49249

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00697 DNA_LIGASE_A1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQYWEEEIEIMSREKLQELQLQRLKKTINIAANSPYYKEVFSKNGITGDSIQSLDDIR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCCCCCHHHHHHHHH
KIPFTTKSDMRANYPFGLVAGDMKRDGVRIHSSSGTTGNPTVIVHSQHDLDSWANLVARC
HCCCCCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHH
LYMVGIRKTDVFQNSSGYGMFTGGLGFQYGAERLGCLTVPAAAGNSKRQIKFISDFKTTA
HHHHCCCHHHEEECCCCCEEEECCCCCHHCHHHCCEEEECCCCCCCCCEEEEEHHHHHHH
LHAIPSYAIRLAEVFQEEGIDPRETTLKTLVIGAEPHTDEQRRKIERMLNVKAYNSFGMT
HHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHEECCCCCE
EMNGPGVAFECQEQNGMHFWEDCYLVEIIDPETGEPVPEGEIGELVLTTLDREMMPLIRY
ECCCCCEEEEECCCCCCEEECCEEEEEEECCCCCCCCCCCCHHHHHHHHHCHHHHHHHHH
RTRDLTRILPGKCPCGRTHLRIDRIKGRSDDMFIIKGVNIFPMQVEKILVQFPELGSNYL
HHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEE
ITLETVNNQDEMIVEVELSDLSTDNYIELEKIRRDIIRQLKDEILVTPKVKLVKKGSLPQ
EEEEEECCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHEEECCCEEEEECCCCCC
SEGKAVRVKDLRDNK
CCCCEEEEECCCCCC
>Mature Secondary Structure 
STQYWEEEIEIMSREKLQELQLQRLKKTINIAANSPYYKEVFSKNGITGDSIQSLDDIR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCCCCCHHHHHHHHH
KIPFTTKSDMRANYPFGLVAGDMKRDGVRIHSSSGTTGNPTVIVHSQHDLDSWANLVARC
HCCCCCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHH
LYMVGIRKTDVFQNSSGYGMFTGGLGFQYGAERLGCLTVPAAAGNSKRQIKFISDFKTTA
HHHHCCCHHHEEECCCCCEEEECCCCCHHCHHHCCEEEECCCCCCCCCEEEEEHHHHHHH
LHAIPSYAIRLAEVFQEEGIDPRETTLKTLVIGAEPHTDEQRRKIERMLNVKAYNSFGMT
HHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHEECCCCCE
EMNGPGVAFECQEQNGMHFWEDCYLVEIIDPETGEPVPEGEIGELVLTTLDREMMPLIRY
ECCCCCEEEEECCCCCCEEECCEEEEEEECCCCCCCCCCCCHHHHHHHHHCHHHHHHHHH
RTRDLTRILPGKCPCGRTHLRIDRIKGRSDDMFIIKGVNIFPMQVEKILVQFPELGSNYL
HHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEE
ITLETVNNQDEMIVEVELSDLSTDNYIELEKIRRDIIRQLKDEILVTPKVKLVKKGSLPQ
EEEEEECCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHEEECCCEEEEECCCCCC
SEGKAVRVKDLRDNK
CCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]