Click here to switch to the map view.

The map label for this gene is rstB [H]

Identifier: 29141796

GI number: 29141796

Start: 1400397

End: 1401701

Strand: Reverse

Name: rstB [H]

Synonym: t1339

Alternate gene names: 29141796

Gene position: 1401701-1400397 (Counterclockwise)

Preceding gene: 29141797

Following gene: 29141795

Centisome position: 29.25

GC content: 53.64

Gene sequence:

>1305_bases
TTGATGAAAAAGCTGTTTGTGCAGTTTTACCTTTTGCTGTTCGTCTGTTTTCTGGTCATGACCTTATTGGTCGGACTGGT
GTACAAATTCACTGCCGAACGCGCGGGCAGGCAATCGCTCGACGATCTGATGAAAAGTTCGCTCTATCTGATGCGCAGCG
AACTGCGGGAAATTCCGCCGCGTGAATGGGGGAAAACGTTAAAAGAGATGGACCTGAACCTCTCTTTCGATTTACGCGTT
GAACCGTTAAATCACTATAAGCTTGATGCCGCCACCACGCAGCGACTGCGTGAAGGCGATATTGTCGCGCTTGACGATCA
ATACACCTTTATTCAGCGTATCCCGCGCAGCCATTACGTCCTGGCGGTTGGCCCGGTTCCCTATCTCTATTTCCTGCATC
AAATGCGACTGCTGGATATCGCCTTAATGGCGCTCATTGCCTTTTCTCTCGCCTTTCCTGTGTTTATCTGGATGCGCCCG
CACTGGCAAGAAATGCTCAGGCTCGAATCCGCCGCGCAGCGCTTTGGCGAGGGTCATTTGACTGAACGCCTGCATTTCGA
TAATGGTTCCAGTTTCGAACGTCTGGGCGTCGCGTTCAACCAGATGGCGGATAACATTAATGCTCTGATCGCCAGTAAAA
AACAGCTGATTGACGGTATCGCCCATGAGCTTCGCACGCCGCTGGTACGTTTACGCTATCGGCTTGAAATGAGTGAAAAT
CTGACTCCGCCGGAATCACAGGCGCTCAATCGCGATATTGGTCAACTGGAAGCGCTGATTGAAGAACTGCTCACCTATGC
GCGCCTTGACCGACCACAAAATGAACTGATGTTGACCAAACCCGACCTCCCCGCCTGGTTGTTCGCGCATTTGCAGGATG
TGCAGAGCGTGAACCCGGAACGCGCCGTAAATCTTCTCACATGTGTAATCGGCGATTATGGCGCGCTTGATATGCGGTTA
ATGTCGCGAGTGCTGGATAACCTGCTGAATAATGCGTTGCGCTACAGCCGTACCACCGTTCAGGTCAGTCTGTTACTTGA
CGGCAGTCAGGCAACGCTGATAGTCGAAGATGATGGCCCCGGCATTGAAGCGGATGCGCGAGAGCGCGTATTTGAACCTT
TTGTCAGACTCGACCCCAGTAGAGACCGGGCGACAGGCGGCTGCGGGCTTGGACTCGCCATCGTACACTCTATCGCTCTG
GCGATGGGCGGTAGCGTCGTCTGTGACGATAGCGAACTGGGCGGCGCAAAATTTAGCTTTCGCTGGCCGGTCTGGCACGC
GATGCCCGACATGACCGCGGCCTGA

Upstream 100 bases:

>100_bases
AGTTGACGCAAAGCCTGCTTTTATAACCCACCAGCGGCGCTGACGGGAAATCTTTTCCGTCAGCGCGCGATAGAGCTAAT
GTATTGAGATCCGGTGGGCG

Downstream 100 bases:

>100_bases
CGTATTTTGCACGCTGGTTGAGCCGCGGGAATTGTGCTATACAGATAGTATGTTGTAACTAAAGTGAGTGATACCATGTC
CCGCTACGATCTCGTTGAAC

Product: sensor protein RstB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MMKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPREWGKTLKEMDLNLSFDLRV
EPLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYVLAVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRP
HWQEMLRLESAAQRFGEGHLTERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSEN
LTPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTKPDLPAWLFAHLQDVQSVNPERAVNLLTCVIGDYGALDMRL
MSRVLDNLLNNALRYSRTTVQVSLLLDGSQATLIVEDDGPGIEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIAL
AMGGSVVCDDSELGGAKFSFRWPVWHAMPDMTAA

Sequences:

>Translated_434_residues
MMKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPREWGKTLKEMDLNLSFDLRV
EPLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYVLAVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRP
HWQEMLRLESAAQRFGEGHLTERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSEN
LTPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTKPDLPAWLFAHLQDVQSVNPERAVNLLTCVIGDYGALDMRL
MSRVLDNLLNNALRYSRTTVQVSLLLDGSQATLIVEDDGPGIEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIAL
AMGGSVVCDDSELGGAKFSFRWPVWHAMPDMTAA
>Mature_434_residues
MMKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPREWGKTLKEMDLNLSFDLRV
EPLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYVLAVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRP
HWQEMLRLESAAQRFGEGHLTERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSEN
LTPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTKPDLPAWLFAHLQDVQSVNPERAVNLLTCVIGDYGALDMRL
MSRVLDNLLNNALRYSRTTVQVSLLLDGSQATLIVEDDGPGIEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIAL
AMGGSVVCDDSELGGAKFSFRWPVWHAMPDMTAA

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=433, Percent_Identity=81.5242494226328, Blast_Score=739, Evalue=0.0,
Organism=Escherichia coli, GI1790346, Length=276, Percent_Identity=32.2463768115942, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789808, Length=274, Percent_Identity=30.6569343065693, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=29.8892988929889, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786783, Length=247, Percent_Identity=31.1740890688259, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786600, Length=228, Percent_Identity=28.5087719298246, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790861, Length=216, Percent_Identity=31.9444444444444, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693157, Length=256, Percent_Identity=27.34375, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789403, Length=236, Percent_Identity=30.9322033898305, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082128, Length=268, Percent_Identity=29.4776119402985, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081816, Length=224, Percent_Identity=27.2321428571429, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788279, Length=292, Percent_Identity=27.0547945205479, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789149, Length=228, Percent_Identity=27.1929824561404, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790551, Length=232, Percent_Identity=29.3103448275862, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787374, Length=243, Percent_Identity=25.1028806584362, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786912, Length=225, Percent_Identity=27.5555555555556, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788549, Length=225, Percent_Identity=25.7777777777778, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49372; Mature: 49372

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
REWGKTLKEMDLNLSFDLRVEPLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYV
HHHHHHHHHHCCCCEEEEEECCCCCEEECHHHHHHHCCCCEEEECCCHHHHHHCCCCCEE
LAVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRPHWQEMLRLESAAQRFGEGHL
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
TERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSEN
HHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTKPDLPAWLFAHLQDVQSVNPE
CCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCHH
RAVNLLTCVIGDYGALDMRLMSRVLDNLLNNALRYSRTTVQVSLLLDGSQATLIVEDDGP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCC
GIEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIALAMGGSVVCDDSELGGAKFSF
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEEE
RWPVWHAMPDMTAA
ECCHHHCCCCCCCC
>Mature Secondary Structure
MMKKLFVQFYLLLFVCFLVMTLLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
REWGKTLKEMDLNLSFDLRVEPLNHYKLDAATTQRLREGDIVALDDQYTFIQRIPRSHYV
HHHHHHHHHHCCCCEEEEEECCCCCEEECHHHHHHHCCCCEEEECCCHHHHHHCCCCCEE
LAVGPVPYLYFLHQMRLLDIALMALIAFSLAFPVFIWMRPHWQEMLRLESAAQRFGEGHL
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
TERLHFDNGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSEN
HHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTPPESQALNRDIGQLEALIEELLTYARLDRPQNELMLTKPDLPAWLFAHLQDVQSVNPE
CCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCHH
RAVNLLTCVIGDYGALDMRLMSRVLDNLLNNALRYSRTTVQVSLLLDGSQATLIVEDDGP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCC
GIEADARERVFEPFVRLDPSRDRATGGCGLGLAIVHSIALAMGGSVVCDDSELGGAKFSF
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEEE
RWPVWHAMPDMTAA
ECCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]