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The map label for this gene is menE [H]

Identifier: 29141073

GI number: 29141073

Start: 634081

End: 635448

Strand: Direct

Name: menE [H]

Synonym: t0558

Alternate gene names: 29141073

Gene position: 634081-635448 (Clockwise)

Preceding gene: 29141072

Following gene: 29141074

Centisome position: 13.23

GC content: 60.31

Gene sequence:

>1368_bases
ATGACATTCACGGACTGGCCGTGGCGCCACTGGCGTCAGGTTCGCAGTCAGGCGCCCGCTCTGCGCCTGAACGATGAAGT
GCTAAGCTGGCGCGCCCTCTGTGAGCGTATTGATGCGCTGGCGGGCGGGTTTGCCGCGCAGGGCGTACGGGAGGGCGACG
GTGTCCTGCTGCGCGCCGGGAATCAGCCGCGCACTCTGTTGGCATGGCTTGCGCTGATGCAGTGCGGCGCGCGGGTTCTG
CCCGTCAACCCGCAGTTGCCCCAGACGTTGCTGGAGGCGCTGGTGCCGAAGTTAACGCTGCGTTTTGCGCTGACACTGGA
AGGCGAAAACGCGTTTTCCGGATTAACCGTGTTGCAGTTGCGGAAAAGTACAGTGGCGTATGCTGTGGCCTGGCAACCGC
AGCGTCTGGTTTCCATGACGCTGACATCGGGTTCCACAGGATTGCCAAAAGCGGCTGTACATACGTGCCAGGCGCATCTT
GCCAGCGCGCAGGGGGTGCTTTCGCTAATGCCGTTTGGCCCTCAGGATGACTGGCTGCTATCGCTGCCGCTGTTTCATGT
ATCCGGGCAGGGTATTATGTGGCGTTGGCTCTTCGCCGGGGCGCGCATGACGGTACGCGATAAGCAACCGCTGGAGCAGA
TGCTGGCAGGTTGTACTCATGCTTCGTTGGTGCCGACGCAGCTGTGGCGGCTGTTGGCGAATCGGGCGGCGGTGACGCTG
AAAGCCGTTTTGCTCGGCGGCGCGGTGATTCCGGTTGAACTCACCGACCAGGCCAGCAAACAAGGGATTCGCTGCTGGTG
CGGGTATGGGCTCACGGAGTTCGCGTCTACGGTATGCGCCAAAGAGGCCGATGGTTCGGATGATGTGGGCGCGCCGCTTC
CCGGCAGAGAGATCAGAATCGTCGATAATGAAGTCTGGCTGCGCGCCGCCAGTATGGCGGAAGGTTACTGGCGTGATGGC
AAACTGATCCCGCTGGTTAATGACGAAGGCTGGTTTGCCACGCGCGATCGCGGCGAGCTGAACCACGGCAGGCTAACCAT
CGCCGGGCGGCTGGACAACTTGTTCTTTAGCGGCGGCGAAGGCATTCAGCCGGAGGAAGTCGAACGCGTTATTAACGCGC
ATCCGCTGGTGCAACAAGCCTTTGTCGTGCCGGTAGAAGATAAAGAGTTTGGCCATCGTCCCGTTGCGGTTGTGGAGTAT
GCATCGCAGGCCGGAGACGTGAACCTCGCTGAATGGGTTCGCGATAAGCTGGCGCGTTTTCAACAGCCGGTTCGCTGGTT
GACGCTGCCGTCAGAACTGAAAAACGGAGGGATTAAAATATCTCGCCGTGCGCTCCAGCAGTGGGTGTGTAAGATCTGTA
AAAATTAA

Upstream 100 bases:

>100_bases
CCGGACTGGATACCTTGCATCTGATGCAGGCGCAACAGGTTCGCCCCTGGCCTGGTAACGCGTTGCCTTGTCTGAAGCGT
GATGAGCTGGAACGACTGTT

Downstream 100 bases:

>100_bases
TGTTAGGTTAATATACAACCATTCCATCGCTATTGCCGTTTTGTTTATCCGTTGATGTGTAACGTGTTATGAACAATCAG
CGTGAAACGGGGGCGCTATG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDALAGGFAAQGVREGDGVLLRAGNQPRTLLAWLALMQCGARVL
PVNPQLPQTLLEALVPKLTLRFALTLEGENAFSGLTVLQLRKSTVAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHL
ASAQGVLSLMPFGPQDDWLLSLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTL
KAVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRIVDNEVWLRAASMAEGYWRDG
KLIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGEGIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEY
ASQAGDVNLAEWVRDKLARFQQPVRWLTLPSELKNGGIKISRRALQQWVCKICKN

Sequences:

>Translated_455_residues
MTFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDALAGGFAAQGVREGDGVLLRAGNQPRTLLAWLALMQCGARVL
PVNPQLPQTLLEALVPKLTLRFALTLEGENAFSGLTVLQLRKSTVAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHL
ASAQGVLSLMPFGPQDDWLLSLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTL
KAVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRIVDNEVWLRAASMAEGYWRDG
KLIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGEGIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEY
ASQAGDVNLAEWVRDKLARFQQPVRWLTLPSELKNGGIKISRRALQQWVCKICKN
>Mature_454_residues
TFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDALAGGFAAQGVREGDGVLLRAGNQPRTLLAWLALMQCGARVLP
VNPQLPQTLLEALVPKLTLRFALTLEGENAFSGLTVLQLRKSTVAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHLA
SAQGVLSLMPFGPQDDWLLSLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTLK
AVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRIVDNEVWLRAASMAEGYWRDGK
LIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGEGIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEYA
SQAGDVNLAEWVRDKLARFQQPVRWLTLPSELKNGGIKISRRALQQWVCKICKN

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=449, Percent_Identity=78.8418708240534, Blast_Score=700, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=495, Percent_Identity=24.040404040404, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788107, Length=516, Percent_Identity=23.8372093023256, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786801, Length=416, Percent_Identity=25.9615384615385, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=455, Percent_Identity=25.4945054945055, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=434, Percent_Identity=25.5760368663594, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=494, Percent_Identity=22.0647773279352, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538037, Length=488, Percent_Identity=24.1803278688525, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=502, Percent_Identity=25.0996015936255, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560308, Length=297, Percent_Identity=26.2626262626263, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24581924, Length=511, Percent_Identity=23.8747553816047, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50281; Mature: 50149

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDALAGGFAAQGVREGDGVLLRAG
CCCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEECC
NQPRTLLAWLALMQCGARVLPVNPQLPQTLLEALVPKLTLRFALTLEGENAFSGLTVLQL
CCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHEEE
RKSTVAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHLASAQGVLSLMPFGPQDDWLL
HHHHEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCCCEE
SLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTL
EEEEEEECCCCEEEEEEHHCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KAVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRI
HHHHHCCEEEEEEECCCHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
VDNEVWLRAASMAEGYWRDGKLIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGE
ECCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCCCEEEEEEEECCCEECCCC
GIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEYASQAGDVNLAEWVRDKLARF
CCCHHHHHHHHHCCCHHHHHEEEEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
QQPVRWLTLPSELKNGGIKISRRALQQWVCKICKN
HCCCEEEECHHHHCCCCEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TFTDWPWRHWRQVRSQAPALRLNDEVLSWRALCERIDALAGGFAAQGVREGDGVLLRAG
CCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEECC
NQPRTLLAWLALMQCGARVLPVNPQLPQTLLEALVPKLTLRFALTLEGENAFSGLTVLQL
CCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHEEE
RKSTVAYAVAWQPQRLVSMTLTSGSTGLPKAAVHTCQAHLASAQGVLSLMPFGPQDDWLL
HHHHEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCCCEE
SLPLFHVSGQGIMWRWLFAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLANRAAVTL
EEEEEEECCCCEEEEEEHHCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KAVLLGGAVIPVELTDQASKQGIRCWCGYGLTEFASTVCAKEADGSDDVGAPLPGREIRI
HHHHHCCEEEEEEECCCHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
VDNEVWLRAASMAEGYWRDGKLIPLVNDEGWFATRDRGELNHGRLTIAGRLDNLFFSGGE
ECCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCCCEEEEEEEECCCEECCCC
GIQPEEVERVINAHPLVQQAFVVPVEDKEFGHRPVAVVEYASQAGDVNLAEWVRDKLARF
CCCHHHHHHHHHCCCHHHHHEEEEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
QQPVRWLTLPSELKNGGIKISRRALQQWVCKICKN
HCCCEEEECHHHHCCCCEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]