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The map label for this gene is yaaF [H]

Identifier: 29140595

GI number: 29140595

Start: 60161

End: 61081

Strand: Direct

Name: yaaF [H]

Synonym: t0053

Alternate gene names: 29140595

Gene position: 60161-61081 (Clockwise)

Preceding gene: 29140594

Following gene: 29140602

Centisome position: 1.26

GC content: 56.35

Gene sequence:

>921_bases
ATGACAGCATCCCTACACATTATTCTTGATACCGATCCGGGCATTGATGACGCGGCTGCGATTGCCGCTGCGCTGTTCGC
CCCCCAGCTTGATCTGCAACTAATAACCACCGTCGCAGGCAATGTTTCCGTTGAAAAAACGACCCGTAATGCGTTGCAGC
TTCTGCATTTCTGGAACAGCGATATCCCACTTGCCCAGGGGGCCGCGACGCCGCTGCTGCGTCCGTTACGCGATGCGGCA
TACGTCCACGGCGAATCCGGTATGGAAGGCTATGATTTTGTCGACCATCAGCGTCAACCGTTGGCGAAGCCTGCGTTTAT
CGCTATCCGGGATGTGTTGATGAACGCCCCGGAGCCCATGACATTGGTGGCGATTGGGCCATTGACCAATATTGCGTTGC
TACTGATGCACTATCCGGAGTGTGCGTGTAACATTCGCCGTCTGGTGTTGATGGGCGGTTCCGCAGGGCGCGGCAATTTT
ACGCCTAATGCTGAATTTAACATTGCTGTCGATCCGGAGGCCGCGGCGCTGGTGTTCCGCAGCGGTCTGGAGATTGTGAT
GTGTGGGCTTGATGTCACGAATCAGGCAATGCTCTCGCCAGATTTTCTGAATAAACTGCCTGCGCTGAATCGCACCGGTA
AGATGTTGCACAGCCTGTTCAACCATTATCGCAGCGGTAGTATGCGCACTGGCGTCCGGATGCACGATCTCTGTGCGATC
GCCTGGCTGGTGCGTCCGGAGCTTTTTACCCTGCAATCCTGTTTTGTGGCCGTAGAAACGCAGGGGGAATATACCGCAGG
CACGACGGTCGTTGATATTGAAGGGCGTCTGGGGCAGCCCGCCAACGCGCAGGTGGCGCTGGCGCTGGATGTGGACGGTT
TTCGTCAGTGGGTGGCTGAGGTGTTTGCCTATGTGCCGTAA

Upstream 100 bases:

>100_bases
TAAGCTAAGTCCGCGTCTGGTGACGGATGACGATGTGCCTGATACCTGTATCAGTCGCGCTGAGTATGAAGATATAAAAC
AATAGGTCAGGAGAGCGTTA

Downstream 100 bases:

>100_bases
TTACATGTTGGCGCCATAATCTCATACTTTGCCGCGATAGAGTCTCTCCGGCCTGCCCACTTTCCCGTAGCTGATCTCCG
CTTCGAGAAAGTTATTTTTC

Product: ribonucleoside hydrolase RihC

Products: uracil; ribose; xanthine; hypoxanthine; adenine; cytosine; guanine [C]

Alternate protein names: Purine/pyrimidine ribonucleoside hydrolase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTASLHIILDTDPGIDDAAAIAAALFAPQLDLQLITTVAGNVSVEKTTRNALQLLHFWNSDIPLAQGAATPLLRPLRDAA
YVHGESGMEGYDFVDHQRQPLAKPAFIAIRDVLMNAPEPMTLVAIGPLTNIALLLMHYPECACNIRRLVLMGGSAGRGNF
TPNAEFNIAVDPEAAALVFRSGLEIVMCGLDVTNQAMLSPDFLNKLPALNRTGKMLHSLFNHYRSGSMRTGVRMHDLCAI
AWLVRPELFTLQSCFVAVETQGEYTAGTTVVDIEGRLGQPANAQVALALDVDGFRQWVAEVFAYVP

Sequences:

>Translated_306_residues
MTASLHIILDTDPGIDDAAAIAAALFAPQLDLQLITTVAGNVSVEKTTRNALQLLHFWNSDIPLAQGAATPLLRPLRDAA
YVHGESGMEGYDFVDHQRQPLAKPAFIAIRDVLMNAPEPMTLVAIGPLTNIALLLMHYPECACNIRRLVLMGGSAGRGNF
TPNAEFNIAVDPEAAALVFRSGLEIVMCGLDVTNQAMLSPDFLNKLPALNRTGKMLHSLFNHYRSGSMRTGVRMHDLCAI
AWLVRPELFTLQSCFVAVETQGEYTAGTTVVDIEGRLGQPANAQVALALDVDGFRQWVAEVFAYVP
>Mature_305_residues
TASLHIILDTDPGIDDAAAIAAALFAPQLDLQLITTVAGNVSVEKTTRNALQLLHFWNSDIPLAQGAATPLLRPLRDAAY
VHGESGMEGYDFVDHQRQPLAKPAFIAIRDVLMNAPEPMTLVAIGPLTNIALLLMHYPECACNIRRLVLMGGSAGRGNFT
PNAEFNIAVDPEAAALVFRSGLEIVMCGLDVTNQAMLSPDFLNKLPALNRTGKMLHSLFNHYRSGSMRTGVRMHDLCAIA
WLVRPELFTLQSCFVAVETQGEYTAGTTVVDIEGRLGQPANAQVALALDVDGFRQWVAEVFAYVP

Specific function: Hydrolyzes both purine and pyrimidine ribonucleosides with a broad-substrate specificity [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786213, Length=299, Percent_Identity=80.6020066889632, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1786871, Length=308, Percent_Identity=43.8311688311688, Blast_Score=237, Evalue=9e-64,
Organism=Escherichia coli, GI1788486, Length=309, Percent_Identity=39.8058252427184, Blast_Score=203, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI32565474, Length=233, Percent_Identity=28.755364806867, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17565698, Length=189, Percent_Identity=32.8042328042328, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320608, Length=241, Percent_Identity=32.7800829875519, Blast_Score=93, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24641837, Length=328, Percent_Identity=31.7073170731707, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24641839, Length=193, Percent_Identity=33.6787564766839, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583915, Length=185, Percent_Identity=35.1351351351351, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022976 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33152; Mature: 33020

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASLHIILDTDPGIDDAAAIAAALFAPQLDLQLITTVAGNVSVEKTTRNALQLLHFWNS
CCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEHHCCCEEEHHHHHHHHHHHHHCC
DIPLAQGAATPLLRPLRDAAYVHGESGMEGYDFVDHQRQPLAKPAFIAIRDVLMNAPEPM
CCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCE
TLVAIGPLTNIALLLMHYPECACNIRRLVLMGGSAGRGNFTPNAEFNIAVDPEAAALVFR
EEEEECCHHHHHHHHHHCCHHHHCCEEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHH
SGLEIVMCGLDVTNQAMLSPDFLNKLPALNRTGKMLHSLFNHYRSGSMRTGVRMHDLCAI
CCCEEEEECCCCCCCHHCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
AWLVRPELFTLQSCFVAVETQGEYTAGTTVVDIEGRLGQPANAQVALALDVDGFRQWVAE
HHHHCCHHHHHEEEEEEEECCCCEECCCEEEEECCCCCCCCCCEEEEEEEHHHHHHHHHH
VFAYVP
HHHCCC
>Mature Secondary Structure 
TASLHIILDTDPGIDDAAAIAAALFAPQLDLQLITTVAGNVSVEKTTRNALQLLHFWNS
CCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEHHCCCEEEHHHHHHHHHHHHHCC
DIPLAQGAATPLLRPLRDAAYVHGESGMEGYDFVDHQRQPLAKPAFIAIRDVLMNAPEPM
CCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCE
TLVAIGPLTNIALLLMHYPECACNIRRLVLMGGSAGRGNFTPNAEFNIAVDPEAAALVFR
EEEEECCHHHHHHHHHHCCHHHHCCEEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHH
SGLEIVMCGLDVTNQAMLSPDFLNKLPALNRTGKMLHSLFNHYRSGSMRTGVRMHDLCAI
CCCEEEEECCCCCCCHHCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
AWLVRPELFTLQSCFVAVETQGEYTAGTTVVDIEGRLGQPANAQVALALDVDGFRQWVAE
HHHHCCHHHHHEEEEEEEECCCCEECCCEEEEECCCCCCCCCCEEEEEEEHHHHHHHHHH
VFAYVP
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; xanthosine; inosine; adenosine; cytidine; guanosine [C]

Specific reaction: uridine + H2O = uracil + ribose xanthosine + H2O = xanthine + ribose inosine + H2O = hypoxanthine + ribose adenosine + H2O = adenine + ribose cytidine + H2O = cytosine + ribose guanosine + H2O = guanine + ribose [C]

General reaction: Nonspecific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA