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The map label for this gene is pucK [H]

Identifier: 28379206

GI number: 28379206

Start: 2410636

End: 2411976

Strand: Direct

Name: pucK [H]

Synonym: lp_2710

Alternate gene names: 28379206

Gene position: 2410636-2411976 (Clockwise)

Preceding gene: 28379205

Following gene: 28379207

Centisome position: 72.87

GC content: 46.53

Gene sequence:

>1341_bases
TTGAAGGAAGTATCCGCAGCACCTAAAATTACCAATGTTAAGGCGGCCATTTTAGGGTTTCAACATCTGTTAGCTATGTA
TTCGGGTGATGTGCTCGTGCCATTATTGATTGGCGCGGCCTTGCATTTCAGCCAAGAACAATTAACTTACCTAGTCTCAA
TCGACATTTTCATGTGTGGGATCGCAACGTTCCTACAATTAAAACGTACACCACTAACAGGAATCGGGCTTCCCGTCGTG
CTTGGCTGTGCCGTTCAAGCCGTCAATCCGTTGATTCAAATCGGTAAAACATATGGGCTGGGGACCATGTATGGCTCAAT
CATTGGTGCTGGTATTTTTATTTTTCTGATTGCCGGCTTATTTTCAAAGATCAAGAACCTCTTTCCACCAGTCGTTACGG
GATCGCTAATCACAATTATTGGATTCACGCTGATTCCGGTCGCCTTCCAAAATCTTGGTGGCGGCGACACCAGTGCCAAG
GATTTCGGTAACTTGCAAGCACTTGGCATTGGTTTTCTCACCATCGCAATTATTTTATTAATTAGCGTGTTTGCACGTGG
CTTCATGAAATCCGTTTCGATTCTGATTGGTATTTTAGTTGGGACTTTGATTGCCGGGGCAATGGGGATGGTTTCTTTAA
AACCCGTCGCTGAAGCTAGCTGGTTCCATTTACCGACCCTCTTTTACTTTGGGACCCCGCACTTTGAATGGTCATCCATT
TTGACCATGATCCTCGTCTCCTTAACAACGATGGTTGAATCGACCGGGGTCTTCTTTGCCCTTGGCGACATTACCGGTCG
TAAAATTGAAGGCGACGATTTAAAACGTGGTTACCGGGCAGAAGGAATTGCCGTCATCCTCGGTGGCTTGTTCAGTACCT
TCCCCTATTCAACGTTTTCTGAAAATGTCGGCGTCGTTCAATTATCTGGTGTCAAGACGCGAAAGCCAATTTACTTTTCC
GCTGCGTTCTTAGTTGTATTGGGCCTACTGCCTAAGATTGGTGCGCTAGCGACGATTATCCCCGACCCAGTACTTGGTGG
CGCCATGGTCGTCATGTTCGGGATCGTTGGCATTCAAGGGATTCGAATGTTAGCTCAAGTCGATTTCCGCAATAACAATA
ATTTACTCGTGGCAGCCGTTTCGATTGGTCTTGGTTTGGGCGTCACGGTGCAAACCAATATCTTCCAATTCTTACCTGGT
GCACTTCAAATCATGTTGAGCAACGGGGTGGTCGTTGGTAGTGTAGCCGCAGTTGGCCTTAACTTACTCTTCAACCGCCA
CGCAGCCGAAAACACGGTTGATGATGAATCAGTCGGTCTCAATGAAACAGAACAACCTTAA

Upstream 100 bases:

>100_bases
AACCCCGTAAAGGTTGGACTATAAGCAAACGAGGTCATCCCGTTTGCCGGGGTGACTTTTTTTATAGGCAAATCAGCTTA
ATCGAGAGAGGAAGATTGAT

Downstream 100 bases:

>100_bases
TTATGACGCTTAAAAGCAGCGGTTCACAGTCCTAATCTGGCAGTGAACCGCTGTTTTTTTGTTCTGCTACTTAATAGCCG
CTCTCATTCTATTGATTGCT

Product: xanthine / uracil transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKEVSAAPKITNVKAAILGFQHLLAMYSGDVLVPLLIGAALHFSQEQLTYLVSIDIFMCGIATFLQLKRTPLTGIGLPVV
LGCAVQAVNPLIQIGKTYGLGTMYGSIIGAGIFIFLIAGLFSKIKNLFPPVVTGSLITIIGFTLIPVAFQNLGGGDTSAK
DFGNLQALGIGFLTIAIILLISVFARGFMKSVSILIGILVGTLIAGAMGMVSLKPVAEASWFHLPTLFYFGTPHFEWSSI
LTMILVSLTTMVESTGVFFALGDITGRKIEGDDLKRGYRAEGIAVILGGLFSTFPYSTFSENVGVVQLSGVKTRKPIYFS
AAFLVVLGLLPKIGALATIIPDPVLGGAMVVMFGIVGIQGIRMLAQVDFRNNNNLLVAAVSIGLGLGVTVQTNIFQFLPG
ALQIMLSNGVVVGSVAAVGLNLLFNRHAAENTVDDESVGLNETEQP

Sequences:

>Translated_446_residues
MKEVSAAPKITNVKAAILGFQHLLAMYSGDVLVPLLIGAALHFSQEQLTYLVSIDIFMCGIATFLQLKRTPLTGIGLPVV
LGCAVQAVNPLIQIGKTYGLGTMYGSIIGAGIFIFLIAGLFSKIKNLFPPVVTGSLITIIGFTLIPVAFQNLGGGDTSAK
DFGNLQALGIGFLTIAIILLISVFARGFMKSVSILIGILVGTLIAGAMGMVSLKPVAEASWFHLPTLFYFGTPHFEWSSI
LTMILVSLTTMVESTGVFFALGDITGRKIEGDDLKRGYRAEGIAVILGGLFSTFPYSTFSENVGVVQLSGVKTRKPIYFS
AAFLVVLGLLPKIGALATIIPDPVLGGAMVVMFGIVGIQGIRMLAQVDFRNNNNLLVAAVSIGLGLGVTVQTNIFQFLPG
ALQIMLSNGVVVGSVAAVGLNLLFNRHAAENTVDDESVGLNETEQP
>Mature_446_residues
MKEVSAAPKITNVKAAILGFQHLLAMYSGDVLVPLLIGAALHFSQEQLTYLVSIDIFMCGIATFLQLKRTPLTGIGLPVV
LGCAVQAVNPLIQIGKTYGLGTMYGSIIGAGIFIFLIAGLFSKIKNLFPPVVTGSLITIIGFTLIPVAFQNLGGGDTSAK
DFGNLQALGIGFLTIAIILLISVFARGFMKSVSILIGILVGTLIAGAMGMVSLKPVAEASWFHLPTLFYFGTPHFEWSSI
LTMILVSLTTMVESTGVFFALGDITGRKIEGDDLKRGYRAEGIAVILGGLFSTFPYSTFSENVGVVQLSGVKTRKPIYFS
AAFLVVLGLLPKIGALATIIPDPVLGGAMVVMFGIVGIQGIRMLAQVDFRNNNNLLVAAVSIGLGLGVTVQTNIFQFLPG
ALQIMLSNGVVVGSVAAVGLNLLFNRHAAENTVDDESVGLNETEQP

Specific function: Transport of xanthine in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680148, Length=430, Percent_Identity=25.3488372093023, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI40316845, Length=430, Percent_Identity=25.3488372093023, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI44680145, Length=459, Percent_Identity=25.2723311546841, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI44680143, Length=190, Percent_Identity=32.1052631578947, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI87082181, Length=435, Percent_Identity=40.4597701149425, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1790087, Length=421, Percent_Identity=33.0166270783848, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI87082178, Length=426, Percent_Identity=30.5164319248826, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1788843, Length=437, Percent_Identity=29.2906178489703, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI87081818, Length=413, Percent_Identity=30.2663438256659, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17558856, Length=219, Percent_Identity=29.2237442922374, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17541904, Length=225, Percent_Identity=26.2222222222222, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17542262, Length=178, Percent_Identity=26.4044943820225, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356175, Length=445, Percent_Identity=27.1910112359551, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017588
- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 47016; Mature: 47016

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9098051; 9384377 [H]