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The map label for this gene is trpA

Identifier: 28378350

GI number: 28378350

Start: 1512415

End: 1513212

Strand: Direct

Name: trpA

Synonym: lp_1658

Alternate gene names: 28378350

Gene position: 1512415-1513212 (Clockwise)

Preceding gene: 28378349

Following gene: 28378351

Centisome position: 45.72

GC content: 49.5

Gene sequence:

>798_bases
TTGATGACTGATTTAACAACAATTTTTAAAAATCATAAGGCGTTTATTGCTTTTGTAGTGGCCGGTGATCCTAATTTTGA
AGCGACAGTTGACCAAGTTGTTGCGTTAGCAGAAGCGGGTTGTGATCTCGTTGAGATTGGCATTCCGTTCTCAGACCCTG
TGGCTGATGGTCCCGAAATTCAGGCCGCTGATCTCCGTGCGTTTGACCAACACATCACACCCCAACGCGTTTTTGAATTG
GTGGTGGCAATTCGAGAGAAGACAACTATTCCACTCGTGTTTTTAACCTATGCGAACATTGTTTATCAGTTTGGTTATGC
TGCCTTTGCCCAGCAGTGTCAAAGTTTGAATGTCGCAGGCTTGATTATTCCCGACATGCCACTAGAAGCCTCTGGTGAAC
TTCGACCAACCTTAGACCACTATGGTATTGCTTTGATTCCATTGATTGCGCCAACGAGTGATGATGCACGCATCGCAGCG
ATTGCCCAGCAGGCGCGGGGCTTCATTTATGTCGTATCATCACTAGGTGTCACGGGGACCCGGCGACACATCACGACTGA
TTTAGCAACACTGGTGGCGAAGATTCGCCATGCGACTACGTTACCTGTCGCAATTGGTTTTGGTATCCATGAGCCAGCTC
AGGCCCAGGCCATGGCACAAATTGCGGACGGTGTCATCGTTGGTAGTGCAGTCGTGCATTTGATTGCGACGCACCAACCA
GCGACAGCAGTCTTAAGGGACTATACGCGACGGATGCGGCGCGCATTGGATGGTCGCACGGCTGATACAGTTGACTGA

Upstream 100 bases:

>100_bases
TTAGCACCAACGATGCGACGTGACCAAACTATTATTTGTACGTTATCCGGTCGTGGAGATAAAGATGTGGCGGCAATTGC
AAAGTATCGAGGGGTGACAA

Downstream 100 bases:

>100_bases
TCTTATTTGTTACGATGTTGTTGTGCCGGGCTACTTCGATTAAATGCTGGGAAACGCTCGGATAATGTGCTAGTCTAAAA
ACAGCTTACTGCTTTGTGGG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MMTDLTTIFKNHKAFIAFVVAGDPNFEATVDQVVALAEAGCDLVEIGIPFSDPVADGPEIQAADLRAFDQHITPQRVFEL
VVAIREKTTIPLVFLTYANIVYQFGYAAFAQQCQSLNVAGLIIPDMPLEASGELRPTLDHYGIALIPLIAPTSDDARIAA
IAQQARGFIYVVSSLGVTGTRRHITTDLATLVAKIRHATTLPVAIGFGIHEPAQAQAMAQIADGVIVGSAVVHLIATHQP
ATAVLRDYTRRMRRALDGRTADTVD

Sequences:

>Translated_265_residues
MMTDLTTIFKNHKAFIAFVVAGDPNFEATVDQVVALAEAGCDLVEIGIPFSDPVADGPEIQAADLRAFDQHITPQRVFEL
VVAIREKTTIPLVFLTYANIVYQFGYAAFAQQCQSLNVAGLIIPDMPLEASGELRPTLDHYGIALIPLIAPTSDDARIAA
IAQQARGFIYVVSSLGVTGTRRHITTDLATLVAKIRHATTLPVAIGFGIHEPAQAQAMAQIADGVIVGSAVVHLIATHQP
ATAVLRDYTRRMRRALDGRTADTVD
>Mature_265_residues
MMTDLTTIFKNHKAFIAFVVAGDPNFEATVDQVVALAEAGCDLVEIGIPFSDPVADGPEIQAADLRAFDQHITPQRVFEL
VVAIREKTTIPLVFLTYANIVYQFGYAAFAQQCQSLNVAGLIIPDMPLEASGELRPTLDHYGIALIPLIAPTSDDARIAA
IAQQARGFIYVVSSLGVTGTRRHITTDLATLVAKIRHATTLPVAIGFGIHEPAQAQAMAQIADGVIVGSAVVHLIATHQP
ATAVLRDYTRRMRRALDGRTADTVD

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=252, Percent_Identity=37.6984126984127, Blast_Score=165, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6321412, Length=229, Percent_Identity=34.9344978165939, Blast_Score=164, Evalue=9e-42,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_LACPL (Q88WH9)

Other databases:

- EMBL:   AL935256
- RefSeq:   NP_785242.1
- ProteinModelPortal:   Q88WH9
- SMR:   Q88WH9
- GeneID:   1061790
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1658
- NMPDR:   fig|220668.1.peg.1376
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   LPLA220668:LP_1658-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28483; Mature: 28483

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 45-45 ACT_SITE 56-56

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTDLTTIFKNHKAFIAFVVAGDPNFEATVDQVVALAEAGCDLVEIGIPFSDPVADGPEI
CCCHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
QAADLRAFDQHITPQRVFELVVAIREKTTIPLVFLTYANIVYQFGYAAFAQQCQSLNVAG
CHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LIIPDMPLEASGELRPTLDHYGIALIPLIAPTSDDARIAAIAQQARGFIYVVSSLGVTGT
EEECCCCCCCCCCCCCCHHHCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCC
RRHITTDLATLVAKIRHATTLPVAIGFGIHEPAQAQAMAQIADGVIVGSAVVHLIATHQP
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCC
ATAVLRDYTRRMRRALDGRTADTVD
HHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMTDLTTIFKNHKAFIAFVVAGDPNFEATVDQVVALAEAGCDLVEIGIPFSDPVADGPEI
CCCHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
QAADLRAFDQHITPQRVFELVVAIREKTTIPLVFLTYANIVYQFGYAAFAQQCQSLNVAG
CHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LIIPDMPLEASGELRPTLDHYGIALIPLIAPTSDDARIAAIAQQARGFIYVVSSLGVTGT
EEECCCCCCCCCCCCCCHHHCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCC
RRHITTDLATLVAKIRHATTLPVAIGFGIHEPAQAQAMAQIADGVIVGSAVVHLIATHQP
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCC
ATAVLRDYTRRMRRALDGRTADTVD
HHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566