Click here to switch to the map view.

The map label for this gene is trpA

Identifier: 28378349

GI number: 28378349

Start: 1511226

End: 1512425

Strand: Direct

Name: trpA

Synonym: lp_1657

Alternate gene names: 28378349

Gene position: 1511226-1512425 (Clockwise)

Preceding gene: 28378348

Following gene: 28378350

Centisome position: 45.68

GC content: 49.83

Gene sequence:

>1200_bases
ATGATTAAAAATCAATCACAGGCGGGGCGTTACGGCGACTTTGGTGGTCAGTACGTTCCAGAAACATTAATGACCGAGCT
ACAGCGGTTGGATCGCGCCTTTCAACATTACCGACAGGACGCGCATTTTCAAGAAGAGTTGACTGACTTGTTGAATAACT
ATGCGAACCGGCCATCGTTGTTGTACCATGCACAACGATTATCAGATCAATTAGGTGGTGCTCAGATTTATTTTAAACGT
GAGGACTTGAACCATACGGGTGCGCACAAGATTAACAATGTCTTGGGGCAGGCACTGTTGGCAAAGAAGATGGGCAAAAA
GCGATTGATTGCTGAAACTGGGGCTGGACAGCACGGGGTCGCAACTGCAACTATTGCTGCTTTGTTCGGGATGGAATGCG
ACGTCTTTATGGGTAAGAAAGACACGAATCGTCAAGCATTGAATGTTTACCGGATGCAACTATTAGGGGCCAAGGTCCAC
CCGGTCACGACTGGTTCAATGGTGCTAAAGGATGCGATCAATGCGGCCTTACAAGAGTGGACGCGGCGCTGTGATGATAC
AGCTTATATCATGGGCTCTGCAACGGGACCACACCCATTTCCAATGATGGTACATGAATTTCAAAGTGTCATCAGTGTAG
AAGCCCGACAGCAGATCCTGGACCAAACAGGTCATTTGCCGGATGCCGTCGTGGCTTGTGTTGGGGGCGGCAGTAACGCC
ATTGGAAGTTTTGCGGCTTTTCTTGATGATACGTCAGTTGAGCTGATTGGCTGTGAGGCAGCGGGTAAGGGTGTTCAAAC
GCCGTTGACGGCTGCGACGATTGAACGTGGTCGGACTGGTATCTTCCATGGCATGAAGTCACTCTTTTTACAAGATAAAG
CCGGACAAATTGCCCCGGTCTATTCAATCTCGGCTGGGCTGGATTACCCGGGCGTCGGACCGGAACATGCCTACCTGGCT
GCGAGTGGCCGGGCGCAATATGTGGGGATCAACGATGACCAAGCAGTACAAGCATTTGAACTGATTGCTAAGGTCGAAGG
GGTGATCTGTGCGATTGAGAGTGCCCATGCAGTCGCCTACGTACAACAATTAGCACCAACGATGCGACGTGACCAAACTA
TTATTTGTACGTTATCCGGTCGTGGAGATAAAGATGTGGCGGCAATTGCAAAGTATCGAGGGGTGACAATTGATGACTGA

Upstream 100 bases:

>100_bases
GCGGGCAATAAAGACCGTGAAAAAATCAATCTAATGGTCCAACGGGCGCACCAAACGGGCGTGTCAACACCATTATTAAC
AGAAATTAGGAGGAATTAGT

Downstream 100 bases:

>100_bases
TTTAACAACAATTTTTAAAAATCATAAGGCGTTTATTGCTTTTGTAGTGGCCGGTGATCCTAATTTTGAAGCGACAGTTG
ACCAAGTTGTTGCGTTAGCA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MIKNQSQAGRYGDFGGQYVPETLMTELQRLDRAFQHYRQDAHFQEELTDLLNNYANRPSLLYHAQRLSDQLGGAQIYFKR
EDLNHTGAHKINNVLGQALLAKKMGKKRLIAETGAGQHGVATATIAALFGMECDVFMGKKDTNRQALNVYRMQLLGAKVH
PVTTGSMVLKDAINAALQEWTRRCDDTAYIMGSATGPHPFPMMVHEFQSVISVEARQQILDQTGHLPDAVVACVGGGSNA
IGSFAAFLDDTSVELIGCEAAGKGVQTPLTAATIERGRTGIFHGMKSLFLQDKAGQIAPVYSISAGLDYPGVGPEHAYLA
ASGRAQYVGINDDQAVQAFELIAKVEGVICAIESAHAVAYVQQLAPTMRRDQTIICTLSGRGDKDVAAIAKYRGVTIDD

Sequences:

>Translated_399_residues
MIKNQSQAGRYGDFGGQYVPETLMTELQRLDRAFQHYRQDAHFQEELTDLLNNYANRPSLLYHAQRLSDQLGGAQIYFKR
EDLNHTGAHKINNVLGQALLAKKMGKKRLIAETGAGQHGVATATIAALFGMECDVFMGKKDTNRQALNVYRMQLLGAKVH
PVTTGSMVLKDAINAALQEWTRRCDDTAYIMGSATGPHPFPMMVHEFQSVISVEARQQILDQTGHLPDAVVACVGGGSNA
IGSFAAFLDDTSVELIGCEAAGKGVQTPLTAATIERGRTGIFHGMKSLFLQDKAGQIAPVYSISAGLDYPGVGPEHAYLA
ASGRAQYVGINDDQAVQAFELIAKVEGVICAIESAHAVAYVQQLAPTMRRDQTIICTLSGRGDKDVAAIAKYRGVTIDD
>Mature_399_residues
MIKNQSQAGRYGDFGGQYVPETLMTELQRLDRAFQHYRQDAHFQEELTDLLNNYANRPSLLYHAQRLSDQLGGAQIYFKR
EDLNHTGAHKINNVLGQALLAKKMGKKRLIAETGAGQHGVATATIAALFGMECDVFMGKKDTNRQALNVYRMQLLGAKVH
PVTTGSMVLKDAINAALQEWTRRCDDTAYIMGSATGPHPFPMMVHEFQSVISVEARQQILDQTGHLPDAVVACVGGGSNA
IGSFAAFLDDTSVELIGCEAAGKGVQTPLTAATIERGRTGIFHGMKSLFLQDKAGQIAPVYSISAGLDYPGVGPEHAYLA
ASGRAQYVGINDDQAVQAFELIAKVEGVICAIESAHAVAYVQQLAPTMRRDQTIICTLSGRGDKDVAAIAKYRGVTIDD

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=388, Percent_Identity=51.5463917525773, Blast_Score=410, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6321412, Length=390, Percent_Identity=53.8461538461538, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_LACPL (Q88WI0)

Other databases:

- EMBL:   AL935256
- RefSeq:   NP_785241.1
- ProteinModelPortal:   Q88WI0
- SMR:   Q88WI0
- GeneID:   1061519
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1657
- NMPDR:   fig|220668.1.peg.1375
- HOGENOM:   HBG303148
- OMA:   IFMGKED
- ProtClustDB:   PRK04346
- BioCyc:   LPLA220668:LP_1657-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43173; Mature: 43173

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNQSQAGRYGDFGGQYVPETLMTELQRLDRAFQHYRQDAHFQEELTDLLNNYANRPSL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHH
LYHAQRLSDQLGGAQIYFKREDLNHTGAHKINNVLGQALLAKKMGKKRLIAETGAGQHGV
HHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCH
ATATIAALFGMECDVFMGKKDTNRQALNVYRMQLLGAKVHPVTTGSMVLKDAINAALQEW
HHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
TRRCDDTAYIMGSATGPHPFPMMVHEFQSVISVEARQQILDQTGHLPDAVVACVGGGSNA
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCHH
IGSFAAFLDDTSVELIGCEAAGKGVQTPLTAATIERGRTGIFHGMKSLFLQDKAGQIAPV
HHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEE
YSISAGLDYPGVGPEHAYLAASGRAQYVGINDDQAVQAFELIAKVEGVICAIESAHAVAY
EEEECCCCCCCCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHEEHHHHHHHHH
VQQLAPTMRRDQTIICTLSGRGDKDVAAIAKYRGVTIDD
HHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIKNQSQAGRYGDFGGQYVPETLMTELQRLDRAFQHYRQDAHFQEELTDLLNNYANRPSL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHH
LYHAQRLSDQLGGAQIYFKREDLNHTGAHKINNVLGQALLAKKMGKKRLIAETGAGQHGV
HHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCH
ATATIAALFGMECDVFMGKKDTNRQALNVYRMQLLGAKVHPVTTGSMVLKDAINAALQEW
HHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
TRRCDDTAYIMGSATGPHPFPMMVHEFQSVISVEARQQILDQTGHLPDAVVACVGGGSNA
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCHH
IGSFAAFLDDTSVELIGCEAAGKGVQTPLTAATIERGRTGIFHGMKSLFLQDKAGQIAPV
HHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEE
YSISAGLDYPGVGPEHAYLAASGRAQYVGINDDQAVQAFELIAKVEGVICAIESAHAVAY
EEEECCCCCCCCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHEEHHHHHHHHH
VQQLAPTMRRDQTIICTLSGRGDKDVAAIAKYRGVTIDD
HHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566