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The map label for this gene is priA [H]

Identifier: 28378315

GI number: 28378315

Start: 1467824

End: 1470241

Strand: Direct

Name: priA [H]

Synonym: lp_1615

Alternate gene names: 28378315

Gene position: 1467824-1470241 (Clockwise)

Preceding gene: 28378314

Following gene: 28378316

Centisome position: 44.37

GC content: 50.45

Gene sequence:

>2418_bases
GTGGCACAAATTGCCCAAGTATTAGTCGATGTGCCCACTATGCAGACAAATCGACCTTATAGTTATCGAATTCCAACCGC
CTGGCAATCACAAGTGCAACCCGGCATGCGCGTCGTGGTGCCGTTCGGCCGTGGTAAACGGCGGGTGCAGGGGTTTGTGT
TAGCTCTTGATGACGTTACTAACTTTGATGGTGAGCTGAAACCAATCGACGCGGTCGTTGACCTAGCACCGGTTCTGAAT
ACTGAAGCCTTAGCACTGACCAAGTGGTTGGCTGCCAGCACGTTTTCATTTCAGATTACCTGTGCACAGACCATGCTGCC
GGCGGTCATGCGTGCAAAGTATGAAAAACAGTTGCGAACAACAGCAACAACGCCCGCGACGATTCGAACGACGCTGTTTG
GGGACCAATCGATGGTGCCGTTTGATGACGTCGCCACGACGCCGGCAATCGTCAGCCAGTTATTACGGCTGCAGGCGGCT
GGGCAAGTCGATGTGTACTACCAAGTTAAAAATCAAGCACGGCGTAAAACGCAAACGGCGGTGAAAGCGGCCTTAACTAA
CGAAGCGCTACAGGCTGCAGTGGACCAGGTACGCGCCGGGGCTACACAACAATTGGCGGTGCTCCAGGGGTTGCTCACTT
TAAATGGTGAACAGCTCTCGCAAAAGGCGTTTTGTACTCGTTTTGATGTCACGGACGGTGCCATTCGTACAGCAGTACAG
AAAGGCTGGCTTAAAAAAGTGGCCGTCGAGGTTTATCGTGATCCCTACGCAACTAGTGATATTAAGACTACCAAGGCGTT
ACAATTGAATGATGAACAGCAAGTGGCCGTCGATCAGATTACGCAAGCCGTTGACCAAGCCGCAACCACCACGTTCTTGT
TAGAGGGTGTGACGGGGAGCGGTAAGACTGAGGTGTACTTGCAATCGATGGCGGCCGCATTGGTACAAGGAAAAACGGCG
TTGATGTTAGTGCCGGAGATTTCACTGACACCCCAAATGGTTCAACGCGTGAAGGGTCGCTTTGGTAAAGCTGTTGCCGT
TTTGCACAGTGGATTGTCCAGTGGTGAGAAGTATGATGAGTGGCGGCGAATCCAACGAGGTGAGGCTCAGGTGGTGGTTG
GCGCACGCTCAGCGGTGTTTGCCCCGCTAAAAAATCTTGGCTTAATCGTGATGGATGAAGAACATGAGAGCACGTATAAG
CAGGATGATGCCCCGCGTTATCATGCTCGGCAAGTGGCTTTATGGCGTAGTCGTTACCATAACTGTCCAGTCGTACTGGG
TTCGGCGACCCCATCGTTAGAAACGCGCGCCCGCGCAGAAAAGGGCGTTTATCAGCGCTTACTGCTTGCGGATCGGGTCA
ATCGACGGCCCCTTCCTAAGGTTTCAATTATTGATATGAAAAAGGAAATGCAACAACACGCTGAAAGCAATTTCTCAGCG
CCACTGTTAGTTGCCTTGCAGGATCGGCTTGAACGTCATGAGCAGAGTGTTCTAATGCTCAATCGGCGGGGCTATTCATC
GTTTGTGTTGTGCCGAGATTGTGGTTTTGTCTTGAAGTGTCCTAATTGTGATATTTCACTCACGCTACACATGGATACGC
ACACGATGAAGTGCCATTACTGCGGTCACGAAGAAGCCATTCCGCGGAATTGTCCGAACTGTCATAGTCGTCAGATCCGT
TATTATGGGACGGGAACTGAAAAAGTCGAAGAAGAATTGCAAGACTTGCTTCCGGACGCTAACATTATTCGGATGGACAA
TGATACGACCCGTAAAAAGGGCGCCCATGCCAAGCTTTTAGCACAGTTTGGGAGTGGTGAAGCTGATATTTTGATTGGTA
CCCAGATGATTGCGAAGGGACTGGATTTTCCTAACGTGACCTTGGTCGGTGTCTTGAATGCTGATACGGCCCTAGGATTG
CCAGATTTTCGCGCCAGTGAACGGACCTTCCAACTATTAACCCAAGTGAGTGGTCGAGCTGGACGGGCGGAAAAGAGTGG
TCACGTGTATATTCAGACATTTAATCCGGATCATTATGCGATTCGGTTTGCACAACACCACGATTACGAAGGCTTTTTCA
AGTATGAGATGCAGATGCGGCACCGGGGGGGCTATCCGCCGTACTACTTTACGGTTCAAATTACAGCGAGTGACTTAGAT
GAGGGGGTAGCGGCCAAGCGCATGTATCAGTTATTGCAATGGTTACGCCCGCGGTTGGCACCGTCAACGATTCTATTAGG
TCCAACGCCCAAGCCGATCGCACGAGTCAATCGCCGTTATTATTATCAAATCGTGATTAAATATAAACAAGAACCCAATT
TAGCTCAGACGCTGACAACGCTACTGCATGAGACGCAGGCGCAACAACGACAAGGGCTACAGATTGGCATTGACGTGGAA
CCCTTACATTTCATGTAA

Upstream 100 bases:

>100_bases
GAATTGACAACGAAAACTGCAATTGCCAAACAGATCTTGATGACGGCAATTGCGGATGGTGCAGGAACACAGGCATGATT
AAGTAGAGAGGAAGAGTTGT

Downstream 100 bases:

>100_bases
TGGGATTAGGAGGAAGTTTGACAATGACATCAATCGTTTTTATGGGAACGCCCGCGTTTGCGGCACCGATTCTCGAAAGT
TTAATTAAAGAACAATACCA

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MAQIAQVLVDVPTMQTNRPYSYRIPTAWQSQVQPGMRVVVPFGRGKRRVQGFVLALDDVTNFDGELKPIDAVVDLAPVLN
TEALALTKWLAASTFSFQITCAQTMLPAVMRAKYEKQLRTTATTPATIRTTLFGDQSMVPFDDVATTPAIVSQLLRLQAA
GQVDVYYQVKNQARRKTQTAVKAALTNEALQAAVDQVRAGATQQLAVLQGLLTLNGEQLSQKAFCTRFDVTDGAIRTAVQ
KGWLKKVAVEVYRDPYATSDIKTTKALQLNDEQQVAVDQITQAVDQAATTTFLLEGVTGSGKTEVYLQSMAAALVQGKTA
LMLVPEISLTPQMVQRVKGRFGKAVAVLHSGLSSGEKYDEWRRIQRGEAQVVVGARSAVFAPLKNLGLIVMDEEHESTYK
QDDAPRYHARQVALWRSRYHNCPVVLGSATPSLETRARAEKGVYQRLLLADRVNRRPLPKVSIIDMKKEMQQHAESNFSA
PLLVALQDRLERHEQSVLMLNRRGYSSFVLCRDCGFVLKCPNCDISLTLHMDTHTMKCHYCGHEEAIPRNCPNCHSRQIR
YYGTGTEKVEEELQDLLPDANIIRMDNDTTRKKGAHAKLLAQFGSGEADILIGTQMIAKGLDFPNVTLVGVLNADTALGL
PDFRASERTFQLLTQVSGRAGRAEKSGHVYIQTFNPDHYAIRFAQHHDYEGFFKYEMQMRHRGGYPPYYFTVQITASDLD
EGVAAKRMYQLLQWLRPRLAPSTILLGPTPKPIARVNRRYYYQIVIKYKQEPNLAQTLTTLLHETQAQQRQGLQIGIDVE
PLHFM

Sequences:

>Translated_805_residues
MAQIAQVLVDVPTMQTNRPYSYRIPTAWQSQVQPGMRVVVPFGRGKRRVQGFVLALDDVTNFDGELKPIDAVVDLAPVLN
TEALALTKWLAASTFSFQITCAQTMLPAVMRAKYEKQLRTTATTPATIRTTLFGDQSMVPFDDVATTPAIVSQLLRLQAA
GQVDVYYQVKNQARRKTQTAVKAALTNEALQAAVDQVRAGATQQLAVLQGLLTLNGEQLSQKAFCTRFDVTDGAIRTAVQ
KGWLKKVAVEVYRDPYATSDIKTTKALQLNDEQQVAVDQITQAVDQAATTTFLLEGVTGSGKTEVYLQSMAAALVQGKTA
LMLVPEISLTPQMVQRVKGRFGKAVAVLHSGLSSGEKYDEWRRIQRGEAQVVVGARSAVFAPLKNLGLIVMDEEHESTYK
QDDAPRYHARQVALWRSRYHNCPVVLGSATPSLETRARAEKGVYQRLLLADRVNRRPLPKVSIIDMKKEMQQHAESNFSA
PLLVALQDRLERHEQSVLMLNRRGYSSFVLCRDCGFVLKCPNCDISLTLHMDTHTMKCHYCGHEEAIPRNCPNCHSRQIR
YYGTGTEKVEEELQDLLPDANIIRMDNDTTRKKGAHAKLLAQFGSGEADILIGTQMIAKGLDFPNVTLVGVLNADTALGL
PDFRASERTFQLLTQVSGRAGRAEKSGHVYIQTFNPDHYAIRFAQHHDYEGFFKYEMQMRHRGGYPPYYFTVQITASDLD
EGVAAKRMYQLLQWLRPRLAPSTILLGPTPKPIARVNRRYYYQIVIKYKQEPNLAQTLTTLLHETQAQQRQGLQIGIDVE
PLHFM
>Mature_804_residues
AQIAQVLVDVPTMQTNRPYSYRIPTAWQSQVQPGMRVVVPFGRGKRRVQGFVLALDDVTNFDGELKPIDAVVDLAPVLNT
EALALTKWLAASTFSFQITCAQTMLPAVMRAKYEKQLRTTATTPATIRTTLFGDQSMVPFDDVATTPAIVSQLLRLQAAG
QVDVYYQVKNQARRKTQTAVKAALTNEALQAAVDQVRAGATQQLAVLQGLLTLNGEQLSQKAFCTRFDVTDGAIRTAVQK
GWLKKVAVEVYRDPYATSDIKTTKALQLNDEQQVAVDQITQAVDQAATTTFLLEGVTGSGKTEVYLQSMAAALVQGKTAL
MLVPEISLTPQMVQRVKGRFGKAVAVLHSGLSSGEKYDEWRRIQRGEAQVVVGARSAVFAPLKNLGLIVMDEEHESTYKQ
DDAPRYHARQVALWRSRYHNCPVVLGSATPSLETRARAEKGVYQRLLLADRVNRRPLPKVSIIDMKKEMQQHAESNFSAP
LLVALQDRLERHEQSVLMLNRRGYSSFVLCRDCGFVLKCPNCDISLTLHMDTHTMKCHYCGHEEAIPRNCPNCHSRQIRY
YGTGTEKVEEELQDLLPDANIIRMDNDTTRKKGAHAKLLAQFGSGEADILIGTQMIAKGLDFPNVTLVGVLNADTALGLP
DFRASERTFQLLTQVSGRAGRAEKSGHVYIQTFNPDHYAIRFAQHHDYEGFFKYEMQMRHRGGYPPYYFTVQITASDLDE
GVAAKRMYQLLQWLRPRLAPSTILLGPTPKPIARVNRRYYYQIVIKYKQEPNLAQTLTTLLHETQAQQRQGLQIGIDVEP
LHFM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=585, Percent_Identity=40, Blast_Score=410, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 90086; Mature: 89954

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQIAQVLVDVPTMQTNRPYSYRIPTAWQSQVQPGMRVVVPFGRGKRRVQGFVLALDDVT
CCHHHHHHHCCCCCCCCCCEEEECCCCHHHHCCCCCEEEEECCCCCCEEEEEEEEEECCC
NFDGELKPIDAVVDLAPVLNTEALALTKWLAASTFSFQITCAQTMLPAVMRAKYEKQLRT
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHH
TATTPATIRTTLFGDQSMVPFDDVATTPAIVSQLLRLQAAGQVDVYYQVKNQARRKTQTA
CCCCCCEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHH
VKAALTNEALQAAVDQVRAGATQQLAVLQGLLTLNGEQLSQKAFCTRFDVTDGAIRTAVQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCHHHHHHHH
KGWLKKVAVEVYRDPYATSDIKTTKALQLNDEQQVAVDQITQAVDQAATTTFLLEGVTGS
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEEECCCCC
GKTEVYLQSMAAALVQGKTALMLVPEISLTPQMVQRVKGRFGKAVAVLHSGLSSGEKYDE
CCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
WRRIQRGEAQVVVGARSAVFAPLKNLGLIVMDEEHESTYKQDDAPRYHARQVALWRSRYH
HHHHHCCCEEEEEECCHHHHHHHHHCCEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHCC
NCPVVLGSATPSLETRARAEKGVYQRLLLADRVNRRPLPKVSIIDMKKEMQQHAESNFSA
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCCC
PLLVALQDRLERHEQSVLMLNRRGYSSFVLCRDCGFVLKCPNCDISLTLHMDTHTMKCHY
HHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCEEECCCCCEEEEEEECCCEEEEEE
CGHEEAIPRNCPNCHSRQIRYYGTGTEKVEEELQDLLPDANIIRMDNDTTRKKGAHAKLL
CCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCEEEECCCCCHHCCCHHHHH
AQFGSGEADILIGTQMIAKGLDFPNVTLVGVLNADTALGLPDFRASERTFQLLTQVSGRA
HHHCCCCCEEEEEHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCC
GRAEKSGHVYIQTFNPDHYAIRFAQHHDYEGFFKYEMQMRHRGGYPPYYFTVQITASDLD
CCCCCCCEEEEEEECCCCEEEEEECCCCCCCHHHEEHHHHHCCCCCCEEEEEEEECCCCC
EGVAAKRMYQLLQWLRPRLAPSTILLGPTPKPIARVNRRYYYQIVIKYKQEPNLAQTLTT
CHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCEEEEEEEEEECCCCCHHHHHHH
LLHETQAQQRQGLQIGIDVEPLHFM
HHHHHHHHHHCCCEEEEECCCCCCC
>Mature Secondary Structure 
AQIAQVLVDVPTMQTNRPYSYRIPTAWQSQVQPGMRVVVPFGRGKRRVQGFVLALDDVT
CHHHHHHHCCCCCCCCCCEEEECCCCHHHHCCCCCEEEEECCCCCCEEEEEEEEEECCC
NFDGELKPIDAVVDLAPVLNTEALALTKWLAASTFSFQITCAQTMLPAVMRAKYEKQLRT
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHH
TATTPATIRTTLFGDQSMVPFDDVATTPAIVSQLLRLQAAGQVDVYYQVKNQARRKTQTA
CCCCCCEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHH
VKAALTNEALQAAVDQVRAGATQQLAVLQGLLTLNGEQLSQKAFCTRFDVTDGAIRTAVQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCHHHHHHHH
KGWLKKVAVEVYRDPYATSDIKTTKALQLNDEQQVAVDQITQAVDQAATTTFLLEGVTGS
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEEECCCCC
GKTEVYLQSMAAALVQGKTALMLVPEISLTPQMVQRVKGRFGKAVAVLHSGLSSGEKYDE
CCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
WRRIQRGEAQVVVGARSAVFAPLKNLGLIVMDEEHESTYKQDDAPRYHARQVALWRSRYH
HHHHHCCCEEEEEECCHHHHHHHHHCCEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHCC
NCPVVLGSATPSLETRARAEKGVYQRLLLADRVNRRPLPKVSIIDMKKEMQQHAESNFSA
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCCC
PLLVALQDRLERHEQSVLMLNRRGYSSFVLCRDCGFVLKCPNCDISLTLHMDTHTMKCHY
HHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCEEECCCCCEEEEEEECCCEEEEEE
CGHEEAIPRNCPNCHSRQIRYYGTGTEKVEEELQDLLPDANIIRMDNDTTRKKGAHAKLL
CCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCEEEECCCCCHHCCCHHHHH
AQFGSGEADILIGTQMIAKGLDFPNVTLVGVLNADTALGLPDFRASERTFQLLTQVSGRA
HHHCCCCCEEEEEHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCC
GRAEKSGHVYIQTFNPDHYAIRFAQHHDYEGFFKYEMQMRHRGGYPPYYFTVQITASDLD
CCCCCCCEEEEEEECCCCEEEEEECCCCCCCHHHEEHHHHHCCCCCCEEEEEEEECCCCC
EGVAAKRMYQLLQWLRPRLAPSTILLGPTPKPIARVNRRYYYQIVIKYKQEPNLAQTLTT
CHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCEEEEEEEEEECCCCCHHHHHHH
LLHETQAQQRQGLQIGIDVEPLHFM
HHHHHHHHHHCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]